Korean Red ginseng prevents endothelial senescence by downregulating the HO-1/NF-κB/miRNA-155-5p/eNOS pathway

Tae-Hoon Kim,Ji-Yoon Kim,Jieun Bae,Young-Mi Kim,Moo-Ho Won,Kwon-Soo Ha,Young-Guen Kwon,Young-Myeong Kim 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.2

Background: Korean Red ginseng extract (KRGE) has beneficial effects on the cardiovascular system by improving endothelial cell function. However, its pharmacological effect on endothelial cell senescence has not been clearly elucidated. Therefore, we examined the effect and molecular mechanism of KRGE on the senescence of human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were grown in normal or KRGE-supplemented medium. Furthermore, they were transfected with heme oxygenase-1 (HO-1) gene or treated with its inhibitor, a NF-κB inhibitor, and a miR-155-5p mimic or inhibitor. Senescence-associated characteristics of endothelial cells were determined by biochemical and immunohistochemical analyses. Results: Treatment of HUVECs with KRGE resulted in delayed onset and progression of senescence-associated characteristics, such as increased lysosomal acidic β-galactosidase and decreased telomerase activity, angiogenic dysfunction, and abnormal cell morphology. KRGE preserved the levels of anti-senescent factors, such as eNOS-derived NO, MnSOD, and cyclins D and A: however, it decreased the levels of senescence-promoting factors, such as ROS, activated NF-κB, endothelial cell inflammation, and p21 expression. The beneficial effects of KRGE were due to the induction of HO-1 and the inhibition of NF-κB-dependent biogenesis of miR-155-5p that led to the downregulation of eNOS. Moreover, treatment with inhibitors of HO-1, NF-κB, and miR-155-5p abolished the anti-senescence effects of KRGE. Conclusion: KRGE delayed or prevented HUVEC senescence through a signaling cascade involving the induction of HO-1, the inhibition of NF-κB-dependent miR-155-5p biogenesis, and the maintenance of the eNOS/NO axis activity, suggesting that it may protect against vascular diseases associated with endothelial senescence.

Synthesis of Sr Doped HoCrO3 Solid Solution Systems and Their Electrical Properties

PARK, SUNG HO,Kwon, Tae Yun 한국공업화학회 2000 Journal of Industrial and Engineering Chemistry Vol.6 No.4

Ho_(1-x)SrxCrO₃ systems, with perovskite structures, were synthesized for x=0, 0.05, 0.1, 0.2 at 1300 ℃ under atmospheric pressure. The results of X-ray analysis reveals that these prepared systems forms orthorhombic structures. Also, the HoCrO₃ systems which were doped with Sr cations on the site of Ho cations, exhibited a linear increase in their cell volume. It was found that the lattice parameter and reduced volume increased as the doping level of Sr^(2+) increased. From the measurements of D.C conductivity, the activation energy obtained from the conductivity dependence on temperature was 0.18 eV. The conduction mechanism would appear to be the hopping model of electron conduction.

역행적 골수정 삽입술을 이용한 대퇴골 과상부 골절의 치료

권진우,신승호,조원호,성기호,김기환,손경태,이상훈,박세원,배종문,권태우 대한골절학회 2003 대한골절학회지 Vol.16 No.4

마이크로프로세서를 利用한 溫度制御

權泰夏,鄭信一,禹鐘鎬 釜山水産大學校 1982 釜山水産大學 硏究報告 Vol.22 No.2

本 論文에서는 마이크로프로세서와 溫度符號化器를 利用해서 溫度를 計數的으로 制御할 수 있는 시스템을 보다 간단히 構成하였으며 上昇기울기, 維持溫度, 降下기울기에 따라 多少의 差는 있으나 制御溫度의 變化幅이 ±0.3% 以內로 比較的 良好하게 制御되었다. bit數가 많은 A/D 變換器를 使用한다면 보다 더 精密한 溫度制御가 可能할 것이다. 現在狀態로도 實驗室의 小容量 뿐 만 아니라 産業用의 大容量 電氣爐의 溫度制御에도 利用될 수 있을 것으로 본다. This paper describes the temperature control system of an electric furnace using a SDK-85 microprocessor system as a control unit. The hardware structure of the control system was very simplified by eliminating mechanical drive portions and employing electronic circuits instead. Moreover, by employing a software method in the system desired temperature on time, i.e., rising rate, maintaining temperature and time, falling rate, can be easily controlled and changed by program. Experimental results showed the successful operation with flexibility. Temperature variation was less than ±0.3% of the desired value and was more accurate on the maintaining temperature region.

浸透水에 의한 모래層의 破壞에 관한 硏究

權武男,李相昊,柳泰夏 慶北大學校 1991 論文集 Vol.51 No.-

In order to investigate seepage failure of sand layer and analyze the effect of the loaded filter constructed on the top of the layer, small scale laboratory tests were carried out on 6 kinds of grain sized sand layers with and without loaded filter of variations of thickness. The results obtained are as follows; 1. The relationship between critical velocity and critical gradient had positive correlation and appears to be linear equation regardless of grain size of the sand. 2. The critical velocity had a trend to increase linearly as grain size of the sand get to be bigger. And the critical velocity in the effective size of 10mm appeared to be about 15 times of that of the 0.2㎜ effective size. 3. The critical velocity was increased according to increase the thickness of loaded filter. And the critical velocity in the 8㎝ thick of loaded filter showed to be 2 times as comparison to the case of no loaded filter. In virtue of the conclusion above, the seepage force can fail sand layer seriously but the stability of sand layers will be able to improve remarkably by construction of loaded filter on the top of the sand layer.

付審判節次에 關한 考察

權泰鎬 청주대학교 대학원 1989 우암논총 Vol.5 No.-

As to the application by the accuser who has objected to the disposition not to institure a public prosecution by the public prosecutor, the High Court shall rule or whether the dispositions are proper or not. This system has been derived from the system of compulsory indictment of West Germany. The West Germany compulsory indictment system is under purpose to secure the legal public prosecution of the case and all the crimes are to be the object thereof and that it shall be ordered to institute public prosecution. Our system however, in contrary to West Germany system, has the function restricting the discretionary indistment like Japan and public prosecution shall be deemed to have been instituted in the case. The claimant is an accuser who has been noticed for the disposition not to institute public prosecution from the public prosecutor. The case eligible for an application shall be limited to the climes under Article 123 to Article 125 of the Criminal Code. An application shall be filed to the High Court which corresponds to the Higher Prosecutors' Office to which the public prosecutor belongs through the Chief Prosecutor of a district Prosecutors' Office to which the public prosecutor who has disposed not to institute Public prosecution in writting within 10 days from the date of receiving the notice of that disposition. If the applcation is reasonable, the High Court shall commit the case to the competent district court for trial. And, if such an application is not reasonable, the High Court shall dismiss it. The case as has been committed to the competent district court for trial shall be applied its maintenance of public prosecution by the advocate who has been disignated by the Court as the person in charge for maintenance of the public prosecution not by the public prosecutor. The advocate appointed as maintenance person of the public prosecution shall exercise, ex officio, the duties of a public prosector in order to maintain public prosecution until the Judgement is finally settled. As a legislative theory of this system I propose the following. 1. to expand objects for this system 2. to effectively connect with the public prosecutors' appeal system, and 3. to revise the procedure so that may meet with the spirit of filing only the written indictment.

CAD를 이용한 양수장 및 배수장설비 설계의 표준화

권병철,권경우,김기길,명태식,박찬호,박귀용 한밭대학교 산업과학기술연구소 1995 논문집 Vol.3 No.-

This paper is to be illustrated automation in the design of Pumping Station and Draining Station. Program for design automation applying Auto LISP, DCL can be used to design and draw simultaneously. The study result are as follows. 1. Optimal design has been introduced and precise design has been possible using CAD. 2. Dialog design is possible with introduction the concept of design automation, and design cost can be reduced greatly.

산림자원관리를 위한 산림정보 데이터베이스 구축

권태호,김태균 대구대학교 과학기술연구소 1999 科學技術硏究 Vol.5 No.5

This study was made an attempt to construct the user-interface system provided with the database of various forest resources information, which could be applicable to the Seongju Experimental Forest of Taegu University. This system was regarded as the effective tool capable of the more rapid query, analysis and update of related data for systematic forest management. Moreover, It would be a useful tool of decision-making in devising and operating the forest management plan. But there would be much room for supplementation and improvement to make the more convenient and powerful system, therefore more concerns and efforts in collecting, revising and updating the data is continuously required.

생태건축의 실현을 위한 목조건축의 역할에 관한 고찰

권태호 世明大學校 1998 世明論叢 Vol.7 No.-

Our current efforts for the propulsion of 'Environmentally Sound and Sustainable Development(ESSD)' is seen as the result from global introspection of the excessive encroachment on the ecosystem under the name of so-called economic development through rapid industrialization as well as urbanizaion. Thus, the concept of the ESSD has emerged as one of the central issues in many disciplines including architecture in which the employement of green, reusable and/or recyclable building materials is strongly emphasied. With this in view, the present paper, paying attention to the failure of modernizing and accommodation korean traditional wood structure due to the rapid industrialization over 40 years, attempts to ealborate and justify the potentials and roles of wood materials for the accomplishment of ecological architecture in Korea. In addition, major associated reserch areas and policy directions are included inthe conclusion and suggestions.

Methanol 자화성 효모에 의한 균체 단백질 생산

권태종,이동희,정호권,최태부 건국대학교 1992 學術誌 Vol.36 No.2

SCP producing yeast JAM -3032 that has high productivity potential with methanol as carbon and energy source was isolated from soil, and conditions for SCP production, nutritional values, and alcohol oxidase of the microorganism were investigated. Isolated strain JAM-3032 was identified as Pichina on the morphological, cultural, physiological and biochemical characteristics. The production of cell mass reached at maximal level after 54 hrs cultivation at 28℃, pH 6.0 in the medium containing methanol 3.0%, yeast extract 0.8%, K2HPO4 0.1%, KH2PO4 0.1%. MgSO4ㆍ7H2O 0.05%, alanine 0.01%, nicotinic acid 0.04%, and biotin 0.04%. Dry cells of Pichia sp. JAM -3032 was composed of proteins 53.3%, carbohydrates 31. 5%, nucleic acids 5.6%, lipids 2.9%, ash 6.4% and amino acid composition of the proteins was satisfied in FAO reference. Biosynthesis of the alcohol oxidase of the strain JAM -3032 was induced by methanol. The enzyme was purified by treatment of ammonium sulfate fractionation, DEAE-Sephadex A-50 ion exchange chromatography and Sepharose 6B gel nitration. The purified enzyme was homogeneous on SDS-PAGE, and molecular weight was estimated to be about 520,000 D that was consisted of 8 subunits containing FAD as coenzyme. The optimal pH and temperature for the enzyme reaction was 7.5 and 37℃, respectively. Activation energy was 17.1Kcal/mol and half inactivating temperature was 40℃. The enzyme activity was inhibited by Ag+, Cu2+ and Hg2+ ions remarkably. The enzyme acted on methanol specifically, and its Km value was 0.89 mM.