http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
氣體一液體 크로마토그래피에 의한 C_1-C_5 탄화수소류의 同時分析과 그 適用
李善行,李華心,徐幀起 경북대학교 과학교육연구소 1989 科學敎育硏究誌 Vol.13 No.-
The 18 components of C_1-C_5 hydrocarbons were well separated on a single, packed column operated around 40℃ within 20 min. The chromatographic column is 25% sebaconitrile/chromosorb P-AW(10m x 0.3㎝, 60-80 mesh). The optimum flow rate was determined by making a simple Van Deemter plot of the number of theoretical plates VS. Iinear gas velocity. The most efficient flow-rate of N_2 carrier gas was at 35㎖/min, and the mumber of theoretical plates was 5,000-10,000. The linearity of calibration curves were checked ranging ppm to % concentration of CH_4 and C_3 H_8. Peak area was used for quantitating the C_1 hydrocarbon through C_5 hydrocarbons, and relative standard deviations of 10 measures were less than 2.1%.
Expression Patterns of Genes Involved in Carotenoid Biosynthesis in Pepper
Ha, Sun-Hwa,Lee, Shin-Woo,Kim, Jong-Guk,Hwang, Young-Soo 한국응용생명화학회 1999 Journal of Applied Biological Chemistry (J. Appl. Vol.42 No.2
To study the regulatory mechanism of isoprenoid (carotenoid) biosynthesis, we have compared the expression patterns of nine isoprenoid biosynthetic genes in Korean red pepper (Capsicum. annuum cv. NocKaung). The expression of geranylgeranyl pyrophosphate synthase gene was initially induced at early ripening stage (I1) and was rather slightly decreased during pepper fruit ripening. The ex-pression of phytoene synthase gene was strongly induced at semi-ripening stage (I2) and the phytoene desaturase transcript was maximally induced at the fully ripened stage (R). Our results suggest that genes encoding two 3-hydroxy-3-methylglutaryl-CoA reductase isozymes (HMGR1 and HMGR2) and farnesyl pyrophosphate synthase might be not so critical in pepper carotenoid biosynthesis but three genes encoding geranylgeranyl pyrophosphate synthase, phytoene synthase and phytoene desaturase were induced in a sequential manner and coordinately regulated during the ripening of pepper fruit.
Sun-Hyung Lim,Sun-Hwa Ha,MinJi Choi,Da-Hye Kim,SangKyu Park,Jong-Yeol Lee,Young-Mi Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Anthocyanins, providing the bright red-orange to blue-violet colors, flavonoid-derived pigments with strong antioxidant activity that have benefits for human health. We isolated RsMYB1, which encodes an R2R3 MYB transcription factor (TF), from red radish plants (Raphanus sativus L.) that accumulate high levels of anthocyanins. RsMYB1 shows higher expression in red radish than in common white radish, in both leaves and roots, at different growth stages. regulatory genes. Transient expression of RsMYB1 in tobacco showed that RsMYB1 is a positive regulator of anthocyanin production. Also, the synergistic effect of RsMYB1 with B-Peru was larger than the effect of Arabidopsis plants stably expressing RsMYB1 produced red pigmentation throughout the plant, accompanied by up-regulation of the six structural and two regulatory genes for anthocyanin production. This broad transcriptional activation of anthocyanin biosynthetic machinery in Arabidopsis included up-regulation of TRANSPARENT TESTA 8, which encodes a bHLH-type TF. These results suggest that overexpression of RsMYB1 promotes anthocyanin production by triggering the expression of endogenous bHLH genes as potential binding partners for RsMYB1. In addition, RsMYB1-overexpressing Arabidopsis plants had a higher antioxidant capacity than did non-transgenic control plants. Taken together, RsMYB1 is an actively positive regulator for anthocyanins biosynthesis in radish plants and it might be one of the best targets for anthocyanin production by single gene manipulation being applicable in diverse plant species.