차나무 callus 의 catechin류 함량분석

오순자,허인옥,한태완 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

본 연구는 차나무의 신초를 이용하여 기내에서 여러가지 배양조건에 따른 캘러스의 catechin류 함량을 분석하여 생산성이 높은 배양조건을 찾고자 실시하였다. 신초배양시 캘러스는 0.5mg/L NAA와 1mg/L BA 조합 처리구에서 가장 잘 유도되었다. 이 조건에서 유도된 캘러스를 가지고 thiamin-HCI, PVP, taxifolin과 quercetin을 농도별로 처리하여 4주간 배양하여 생장 및 catechin류 함량을 조사하였다. 캘러스의 생장은 30mg/L thiamin-HCL, 200mg/L PVP, 1 mM taxifolin, 0.01 mM quercetin처리시 대조구에 비해 2~3배의 생장를 보였다. 차나무 잎의 주성분인 catechin류를 분석하고자 신초와 여러조건에서 배양된 캘러스를 가지고 분석한 결과를 보면, 캘러스에서 높은 함량을 보였다. 또한 신초에서의 catechin류는 EGC, EGCG, ECG등은 검출되었으나, EC는 검출되지 않았다. 그러나 배양된 캘러스에서는 4가지 성분이 모두 검출되었다. 각각의 성분들의 최적 배양조건을 보면, EGC는 500mg/L PVP, EC는 100mM quercetin, EGCG는 10 mg/L thiamin-HCI, ECG는 10mM taxifolin처리구에서 가장 높은 함량을 보였다. This study aimed to measure the growth of callus cultured under MS medium with thiamin-HCI as a vitamine. PVP as the antioxidant. taxifolin and quercetin as precursors of catechins and to select the clone producing catechins highly. Callus induction from Thea sinensis young leaf was great in the MS medium with 0.5mg/L NAA and 1 mg/L BA. When components of callus were compared with those of young leaf. Four kinds of catechins(EC, EGC, EGCG, ECG) were detected in cultured callus whereas EC not in young leaf. Catechins were detected most in the medium with 10mg/L thiamin-HCI. Expecially, EC was most as a 6.39mg/g. The content of catechins in PVP treatment was not different each other but was higher in 100 mg/L and 200 mg/L PVP than in control. When the callus was cultured in the medium with taxifolin and quercetin the caffeine content was lower than in the medium with not them. Also, the content of catechins in taxifolin and quercetin treatment was not different each other but it was highest in 10mM taxifolin and 100mM quercetin treatment.

산채류의보존과 일본식 절임

오영애,하귀현,박인경,김미향,김미정,김미경,정자림,이명숙,김순동 대구효성가톨릭대학교 식품과학연구소 1992 식품과학지 Vol.4 No.-

This paper showed the summarization of the preservative method of wild vegetables in the house and the precessing method of Japanese pickles. The preservative methods of wild vegetables were explained devided into long, short term, fresh state, heat treatment and drying. Salt, soybean fermented sauce, soybean fermented paste, lees of refined rice wine and residue in the preparation of bean curd were used as the materials of Japanese pickles.

한라산 국립공원 지역의 식생과 식물상

오순자,고정군,고석찬 제주대학교 환경연구소 2000 환경연구논문집 : 제주대 Vol.8 No.-

충수염의 임상적 확신도에 따른 초음파의 유용성 : Correlation with Level of Initial Clinical Diagnostic Confidence

송순영,김삼수,구장홍,이은자,이종인,정진호,김진영,오화은,문원진,한헌 대한초음파의학회 2002 ULTRASONOGRAPHY Vol.21 No.3

Atropa belladonna 모상근의 생장량과 tropane alkaloid 생산에 관한 연구

허인옥,오순자,한태완,김철수 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.1

Agrobacterium rhizogenes strain R1000에 의해 유도된 Atropa belladonna 모상근을 배지별, NAA, IBA, IAA, 2,4-D를 농도별(0.01-100 mg/l)로 첨가한 NN액체배지에서 생장량과 tropane alkaloid 함량을 HPLC로 이용하여 정량하였다. 모상근 배양 30일째의 각 auxin의 농도별에 따른 생장율은 고농도보다 저농도일때 대체적으로 양호하였으나, 0.1mg/1 2,4-D농도에서는 callus화 하였고 10mg/l 2,4-D, 1mg/l IBA일때 2.96g, 2.36g으로 대조구보다 2.3배의 생장량을 보였다. 농도별에 따른 Tropane alkaloid 함량은 atropine이 대조구 0.141%, 원식물체가 0.059%로 나타났으며, 0.01mg/l NAA와 0.1mg/l IAA에서 배양한 모상근이 각각 1.378%, 1.814%의 생산을 보여 대조구와 원식물체보다 9-30배의 생산을 보였다. 또한 scopolamine 함량은 대조구와 원식물체는 각각 0.109%. 0.050%에 비해 0.01mg/l NAA에서 배양한 모상근에서 0.505%로 대조구 원식물체보다 4-10배의 생산량을 보였다. The study aimed to measure growth rate of Atropa belladonna hairy root cultured under medium, various auxin(NAA, IBA, IAA, 2.4-D) and auxin concentration(0.01-100mg/l) and investigated tropane alkaloid content using HPLC. The growth rate of hairy root cultured for 30 days in MS and NN liquid medium were higher in NN medium as 3.51g than MS medium, growth rate of belladonna hairy root cultured in NN medium added various auxin and auxin concentration was the most effective in low concentration than high concentration, especially, was best in the medium supplemented with 0.01mg/l 2,4-D. Atropine content of hairy root cultured in 0.01mg/l and 0.1mg/l IAA produced 9-30 times than control and normal plant. scopolamine measured each 0.109%, 0.050% in control and normal plant, hairy root cultured in 0.01mg/l NAA produced 4-10 times than control and normal plant.

Ethyl benzoate 合成에 미치는 觸媒의 影響에 對한 小考

김인숙,오세종,유경희,허순자 梨花女子大學校 藥學硏究所 1967 梨花藥學硏究 Vol.- No.8

The concentrated sulfuric acid and hydrogen chloride gas which are strong acid by the Bronsted concepts, have been between benzoic acid and ethyl alcohol. In this paper we have studied about comparing with the yield and rate of the catalytic reactions on above two catalysers.

여름철 자연조건에서 온주밀감 잎 광계ll의 광억제

고석찬,오순자,박영철 제주대학교 생명과학연구소 1998 제주생명과학연구 Vol.1 No.-

The street effects of light, temperature and relative humidity in natural conditions were studied on Satsuma mandarin plants(citrus unshiu Marc.) growing in the field during the summer season. ChlorophyII fluorescence induction of leaves was used to characterize the stress effects. Photoinhibition of photosystem II (PS II) was caused in mid-day conditions; ratio of variable to maximum fluorescene (Fv/Fm) decreased remarkably with increased initial fluorescene (Fo) and decreased maximum fluorescene (Fm). However, it was fully recovered with the onset of night time, indicating that it is not fatal for growth of these plants. Photochemical efficiency of PS II, Fv/Fm, showed significant correlation with light intensity, temperature and relative humidity under natural conditions. Especially, under the supraoptimal conditions (light intensity above 1,000 μmole/m²/sec, temperature above 30.5℃ or relative humidity below 71.2%) in summer, it showed significant correlation with only relative humidity. This results suggest that relative humidity is a limiting factor for photosynthesis of Satsuma mandarin plants under field conditions in summer.

대구시 하수도 설계를 위한 기본 조사연구

조병하,이상렵,이순탁,함능수,나인엽,오창언,김자홍 경북대학교 1971 生産技術 Vol.5 No.-

This study is a "reconnaissance" of "preliminary" grade survery with the objectives of compiling and analyzing drainage systems, river pollution, hydraulic and hydrologic characteristics of sewage, and conservation of public water supplies for design of sewer systems in Taegu. In addition, it contains basis information for design of new sanitary sewage and drainage systems for Taegu. The results are shown as follows;1) Before attempting to design a sewage system, it was necessary to set up reliable standards for estinating sewage amounts. 2) The results of analysis and examination of Taegu's drainage systems. 3) The investigation of "sewage quality and river pollution in Taegu. 4) In order to maintain the B.O.D. value of the Kumho River below 8 ppm, a sewage treatment plant will be necessary. The basic information necessary in planning a treatment plant is given. This study is a summary of reseach that was conducted jointly by Kyung-Pook National Univ ersity's Insttute of Industrial Sciences and Young-Nam Universty's Institute of Industrial Sciences and Young-Nam University's Department of Civil Engineering. The study was begun on Novemver 4,1969 and completed on November 3,1970. By an agreement between the United States Agency for International Development and the Ministry of Science and Technology of the Republic of Korea, the work was financial out of the MO ST/USAID Trust Fnnd.

Confrontation between the Two Worlds in "The Bear" by William Faulkner

Oh, Soon Ja 한국현대영어영문학회 1991 현대영어영문학 Vol.33 No.1

두릅나무과 식물의 SOD 활성과 광계 2 의 광화학적 효율에 미치는 온도 스트레스와 Paraquat 의 영향

오순자(Soon Ja Oh),고정군(Jung Goon Koh),김응식(Eung Sik Kim),오문유(Moon You Oh),고석찬(Suck Chan Koh) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.2

The effects of temperature stress and paraquat on the superoxide dismutase(SOD) activity and the photochemical efficiency of photosystem Ⅱ were studied in the leaves of Araliaceae plants. The SOD activity of Acanthopanax koreanum leaf discs increased at 4℃ and 28℃, and increased significantly at 4℃, 28℃ and 35℃ in the presence of paraquat. However, the SOD activity of Dendropanax morbifera leaf discs decreased at 4℃, 28℃ and 35℃ regardless of paraquat treatment. The photochemical efficiency of photosystem Ⅱ, Fv/Fm, of leaf discs of A. koreanum and D. morbifera fell remarkable at 35℃. IN the presence of paraquat, the Fv/Fm values fell slightly at 4℃ in A. koreanum leaf discs and at 35℃, in D. morbifera leaf discs. These results indicate that A. koreanum plants are more resistant to temperature stress or oxidative stress than D. morbifera plants although their photochemical efiiciency falls slightly at 4℃ in the presence of paraquat.