석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율

임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.

The soluble expression and purification of Receptor activator of nuclear factor-kappa B ligand using E. coli

Sol-Ji PARK,Se-Hoon LEE,Kap Seong CHOI,Young-Jin SON 한국생물공학회 2013 한국생물공학회 학술대회 Vol.2013 No.10

Vitamin D Deficiency in Chronic Hepatitis C Patients: Cause or Result?

( Sol Ji Park ),( Dae Won Jun ),( Sang Bong Ahn ),( Jae Yoon Jung ),( Joo Hyun Sohn ),( Seok-yong Lee ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Tons of reports showed prevalence of vitamin deficiency is high in chronic hepatic C patients (CHC). But there is no clear mechanism of vitamin deficiency in CHC. We investigate to find risk factor affecting serum vitamin D level in CHC treatment population. Methods: Fifty-eight CHC patients treated with pegylated-interferon and ribavirin. Serum vitamin D, amount of ultra violet exposure, and HCV-RNA PCR level were evaluated. Results: Forty three (74.1%) among 58 subjects showed vitamin D deficiency (≤20ng/mL) at the baseline. Serum vitamin D level did not change after treatment (16.9 ng/ml vs 17.6 ng/ml, p=0.524). Sustained viral response (SVR) was achieved by 86.2% (50 patients), while 13.8% (8 patients) failed to SVR. Serum vitamin D level did not different after treatment in both SVR (17.1ng/mL vs 17.5ng/mL, p=0.711) and non-SVR group (16.0ng/mL vs 18.1ng/mL, p=0.419). SVR was not different according to baseline vitamin D level. SVR failure was 33.3% in vitamin D < 10 ng/ml, 11.8% in vitamin D < 20 ng/ml, 6.7% in vitamin D ≥ 20 ng/ml. SVR was not risk factor regarding normalization of serum vitamin D level. Estimated amount of UV exposure was risk factor for normalization of serum vitamin D level in multivariate analysis. Conclusions: Serum vitamin D level did not changed after successful treatment in patients with CHC. Only amount of UV exposure was risk factor for normalization of serum vitamin D level.

Analysis of risk factors associated with survival in human epidermal growth factor receptor 2-positive ductal carcinoma in situ using Korean Breast Cancer Society Database

Sol Ji Ahn,Chang Ik Yoon,Pill Sun Paik,Tae-Kyung Yoo,Namsun Park,Eun Sook Lee,Jung Eun Choi,Joon Jeong,Hyun Jo Youn,Woo-Chan Park 대한외과학회 2021 Annals of Surgical Treatment and Research(ASRT) Vol.101 No.6

Purpose: This study was performed to identify the risk of mortality in patients diagnosed with human epidermal growth factor receptor 2 (HER2)-positive ductal carcinoma in situ (DCIS). Methods: We selected 2,592 patients with HER2-positive DCIS from Korean Breast Cancer Society (KBCS) database between January 1997 and December 2019. Patients who received neoadjuvant chemotherapy were excluded. Logistic regression analysis was used to determine the association between clinical factors and overall death after adjusting for tumor and clinical characteristics. Mortality data were modified using the Statistics Korea data. Results: Thirty deaths (1.2%) were identified out of 2,592 patients in the KBCS database. In the univariate logistic regression analysis, older age, higher body mass index (BMI), type of breast surgery (mastectomy), estrogen receptor-negative, progesterone receptor-negative, and exposure to endocrine therapy were significant clinical factors associated with death. In the multivariate analysis, age (hazard ratio [HR], 1.062; 95% confidence interval [CI], 1.015–1.111; P = 0.006), BMI (HR, 1.179; 95% CI, 1.032–1.347, P = 0.016), breast surgery type (mastectomy vs. lumpectomy; HR, 0.285; 95% CI, 0.096–0.844; P = 0.024), and endocrine therapy (HR, 0.314; 95% CI, 0.099–0.995; P = 0.049) were significant risk factors for mortality. Conclusion: Advanced age, higher BMI, mastectomy, and the absence of endocrine therapy were factors associated with poor survival of patients with HER2-positive DCIS. This finding requires further validation combined with additional analysis of large databases.

Oxamflatin Improves Developmental Competence of Porcine Somatic Cell Nuclear Transfer Embryos

Park, Sol-Ji,Park, Hee-Jung,Koo, Ok-Jae,Choi, Woo-Jae,Moon, Joon-ho,Kwon, Dae-Kee,Kang, Jung-Taek,Kim, Sujin,Choi, Ji-Yei,Jang, Goo,Lee, Byeong-Chun Mary Ann Liebert 2012 Cellular reprogramming Vol.14 No.5

<P>Abstract Aberrant epigenetic nuclear reprogramming of somatic nuclei is a major cause of low success in cloning. It has been demonstrated that treatment of histone deacetylase inhibitors (HDACi) enhances developmental potential of somatic cell nuclear transfer (SCNT) embryos by alteration of epigenetic status. The aim of the present study was to investigate the effect of oxamflatin, a novel HDACi, on the developmental competence of porcine SCNT embryos. Treatment with 1???관M oxamflatin for 9???h after activation of SCNT embryos increased both in vitro and in vivo developmental competence. Treatment of SCNT embryos with 1???관M oxamflatin significantly increased blastocyst rate and total cell number in blastocysts (33.3±6.0 and 73.1±1.6, respectively) than that of controls (10.3±3.7 and 54.1±3.5, respectively) or scriptaid (16.4±4.6 and 64.4±2.1, respectively). Moreover, oxamflatin showed significant higher overall cloning efficiency from 0.9% to 3.2%, whereas scriptaid demonstrated 0% to 1.8%. In conclusion, these results indicate that oxamflatin treatment improves the developmental competence of porcine SCNT embryos.</P>

Short-term treatment with 6-DMAP and demecolcine improves developmental competence of electrically or Thi/DTT-activated porcine parthenogenetic embryos

Park, Sol Ji,Koo, Ok Jae,Kwon, Dae Kee,Gomez, Ma Ninia Limas,Kang, Jung Taek,Atikuzzaman, Mohammad,Kim, Su Jin,Jang, Goo,Lee, Byeong Chun Cambridge University Press 2011 Zygote Vol.19 No.1

<B>Summary</B><P>Treatment with 6-dimethylaminopurine (6-DMAP) or demecolcine (DE) for several (at least 2) hours after artificial activation is known to improve <I>in vitro</I> development of porcine embryos. However, several reports have also shown that treatments with these chemicals induce apoptosis. The aim of this study was to find out whether short-term treatment with 6-DMAP and DE combined with electrical or thimerosal/dithiothreitol (Thi/DTT) activation had a beneficial effect on development of parthenogenetically activated porcine oocytes. We additionally treated embryos with 6-DMAP (2 mM) and/or DE (0.4 μg/ml) for a short time (40 min) after an electrical pulse (EP) or Thi/DTT. As a result, short-term treatment with 6-DMAP and DE successfully induced development of electrically or Thi/DTT-activated porcine parthenogenetic embryos with no significant difference in cleavage rate, blastocyst formation rate and total cell number compared with long-term treatment. To find optimal activation protocol, cleavage rate, blastocyst formation rate and total cell number were compared between EP and Thi/DTT treatments. Thi/DTT + 6-DMAP + DE showed significantly higher blastocyst formation rate (36.1 ± 3.5%) and total cell number (46.9 ± 1.0) than other groups (EP + 6-DMAP + DE, EP + Thi/DTT + 6-DMAP + DE: 23.3 ± 3.0%, 42.2 ± 1.1 and 17.2 ± 2.7%, 36.7 ± 1.5, respectively). In conclusion, this study demonstrates that short-term treatment with 6-DMAP and DE is as effective as the standard long-term treatment and Thi/DTT + 6-DMAP + DE exerts a synergistic effect.</P>

Functional improvement of porcine neonatal pancreatic cell clusters via conformal encapsulation using an air-driven encapsulator

Park, Sol Ji,Shin, Soojeong,Koo, Ok Jae,Moon, Joon Ho,Jang, Goo,Ahn, Curie,Lee, Byeong Chun,Yoo, Young Je Korean Society for Biochemistry and Molecular Bion 2012 Experimental and molecular medicine Vol.44 No.1

Transplantation of islet cells into diabetic patients is a promising therapy, provided that the islet cells are able to evade host immune rejection. With improved islet viability, this strategy may effectively reverse diabetes. We applied 2% calcium alginate to generate small and large capsules to encapsulate porcine neonatal pancreatic cell clusters (NPCCs) using an air-driven encapsulator. After encapsulation, the viability was assessed at 1, 4, 7, 14 and 28 days and secretion of functional insulin in response to glucose stimulation were tested at days 14 and 28. Selective permeability of the small alginate capsules was confirmed using various sizes of isothiocyanate-labeled dextran (FITC-dextran). Encapsulation of NPCCs was performed without islet protrusion in the small and large capsules. The viability of NPCCs in all experimental groups was greater than 90% at day 1 and then gradually decreased after day 7. The NPCCs encapsulated in large capsules showed significantly lower viability ($79.50{\pm}2.88%$) than that of naive NPCCs and NPCCs in small capsule ($86.83{\pm}2.32%$, $87.67{\pm}2.07%$, respectively) at day 7. The viability of naive NPCCs decreased rapidly at day 14 ($75.67{\pm}1.75%$), whereas the NPCCs encapsulated in small capsules maintained ($82.0{\pm}2.19%$). After 14 and 28 days NPCCs' function in small capsules ($2.67{\pm}0.09$ and $2.13{\pm}0.09$) was conserved better compared to that of naive NPCCs ($2.04{\pm}0.25$ and $1.53{\pm}0.32$, respectively) and NPCCs in large capsules ($2.04{\pm}0.34$ and $1.13{\pm}0.10$, respectively), as assessed by a stimulation index. The small capsules also demonstrated selective permeability. With this encapsulation technique, small capsules improved the viability and insulin secretion of NPCCs without islet protrusion.

Effect of Vitamin D on the Treatment of Atopic Dermatitis With Consideration of Heterogeneities: Meta-Analysis of Randomized Controlled Trials

Park Ji Soo,Kim Mina,Sol In Suk,Lee Kyung Suk,Park Suyeon,Yang Hyeon-Jong,Lee Eun 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.2

Various therapeutic approaches, including supplemental nutritional support, have been tried for the treatment of atopic dermatitis (AD). Previous studies have reported the role of vitamin D in the treatment of AD with inconsistent results. The aim of this study was to evaluate the effectiveness of vitamin D in the treatment of AD, with considerations on the heterogeneities of AD. Randomized controlled trials (RCTs) on the efficacy of vitamin D supplementation for AD treatment, published before June 30, 2021 were identified in the PubMed, EMBASE, MEDLINE, and Cochrane Library databases. The quality of evidence was assessed using the Grading of Recommendations, Assessment, Development and Evaluation system. This meta-analysis included 5 RCTs with 304 cases of AD. We found that vitamin D supplementation did not decrease AD severity, even when AD was classified as severe vs non-severe. However, vitamin D supplementation was found to be effective in the treatment of AD in RCTs that included both children and adults, but not in those that included only children. Geographic location was associated with a significant difference in the therapeutic effect of vitamin D supplementation. Moreover, vitamin D supplementation of > 2,000 IU/day decreased AD severity, but supplementation ≤ 2,000 IU/day did not. Vitamin D supplementation, in general, was not effective for the treatment of AD. However, vitamin D supplementation might provide a therapeutic effect depending on the geographic location and dose of supplementation. The results of the present meta-analysis suggest that vitamin D supplementation might be targeted for patients with AD who may benefit from vitamin D supplementation.

Isolation of Megastigmane Sesquiterpenes from the Silkworm (Bombyx mori L.) Droppings and Their Promotion Activity on HO-1 and SIRT1

Ji-Hae Park,Do-Gyeong Lee,Seung-Woo Yeon,Hyuk-Sang Kwon,Jong-Hee Ko,Dong-Jin Shin,Han-Sol Park,Yong-Soon Kim,Myun-Ho Bang,NAM-IN BAEK 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.4

The silkworm (Bombyx mori L.) droppings were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned in succession with EtOAc, n-BuOH, and H_2O. From the EtOAc fraction, five megastigmane sesquiterpenes were isolated through repeated silica gel and ODS column chromatography. According to the results of spectroscopic data, such as NMR, MS, and IR, the chemical structures of the isolated compounds were determined as (3S,5R,8R)-3,5-dihydroxymegastigma-6,7-dien-9-one (1), (S)-dehydrovomifoliol (2), (6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one (3), (3S,5R,6S,7E)-3,5,6-trihydroxy-7-megastigmen-9-one (4), (6R,9R)-9-hydroxy-4-megastigmen-3-one (5). Compounds 2 through 5 were isolated for the first time from silkworm droppings. GC/MS analysis indicated silkworm powder contained compound 3, and mulberry leaves contained compound 4. Compounds 1 and 5 increased the expression of heme oxygenase-1 and SIRT1 in HepG2 and HEK239 cells, respectively. Heme oxygenase-1 is considered to be an antioxidant enzyme that catabolizes heme to carbon monoxide, free iron and biliverdin, while SIRT1 is the mammalian homologue of the yeast silent information regulator (Sir)-2, which are involved in the suppression of inflammatory mediators or factors that may be used to improve atopy-related symptoms.

Pleomorphic Dermal Sarcoma on Burn Scar: A Case Report

Park Jin Sol,Park Ji-Ung 대한창상학회 2023 Journal of Wound Management and Research Vol.19 No.1

Pleomorphic dermal sarcoma (PDS), a cutaneous form of undifferentiated pleomorphic sarcoma, is a rare, aggressive sarcoma that is known to develop on locations exposed to ultraviolet light. Here, we describe a unique case of PDS that developed on a burn scar with little ultraviolet light exposure. Most neoplasms from burn scars have an ectodermal origin, but our case was distinctive in that the neoplasm originated from the mesoderm. The 52-year-old patient, who had sustained a burn injury 40 years earlier, had suffered a small abrasion on his burn scar 3 months prior; the scar underwent rapid exophytic growth after repeated insult. After wide excision of the tumor with a clear resection margin and reconstruction with a skin graft, neither recurrence nor distant metastasis was reported. To our knowledge, this is the first case of diagnosis and successful management of a PDS originating from a burn scar. We discuss the diagnosis and management of this rare type of sarcoma, as well as its probable pathophysiologic mechanism related to burn scars. This case highlights the need for clinicians to promptly investigate patients with chronic wounds on their burn scars and to include PDS as one of the differential diagnoses.