Therapeutic Effects of Recombinant Human Epidermal Growth Factor (rhEGF) in a Murine Model of Concurrent Chemo- and Radiotherapy-Induced Oral Mucositis

RYU, Seung-Hee,KANG, Ki Mun,MOON, Soo Young,CHAI, Gyu Young,HONG, Joon Pio,CHO, Kyoung-Oh,KANG, Mun-Il,CHOI, Eun Kyung,LEE, Sang-wook Journal of Radiation Research Editorial Committee 2010 JOURNAL OF RADIATION RESEARCH Vol.51 No.5

<P>Concurrent chemotherapy with radiotherapy (CCRT) has been applied for the treatment of advanced stage of head and neck cancer patients. However CCRT is associated with several complications including mucositis, dermatitis, stomatitis, etc. This study was conducted to evaluate the therapeutic effect of systemically administrated recombinant human epidermal growth factor (rhEGF) in CCRT-induced oral mucositis in a mouse model. Oral mucositis was induced in male BALB/c mice through combination treatment with cisplatin (11 mg/kg, i.p.) and irradiation (17 Gy) of the head and neck area. rhEGF (1.0 mg/kg/day for consecutive 3 days) was administered systemically, and the therapeutic effect was determined by histological evaluation of the oral mucosa. To elucidate optimal dose of rhEGF on CCRT-induced mucositis, various concentrations (0.04–3 mg/kg) of rhEGF were injected for 3 days. Systemic rhEGF administration accelerated the recovery of body weight. Histologically, rhEGF-treated mice showed significantly increased epithelial cell layer thickness, basal cell number, and expression of Ki-67 compared to control mice. Most effective dose was 1 mg/kg among other doses tested. Systemic administration of 1 mg/kg of rhEGF reduces the severity of oral mucositis induced by CCRT in a mouse model, suggesting that rhEGF can be used for treating CCRT-induced mucositis during the cancer treatment.</P>

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

악골낭종에 대한 임상적 연구

문철,이동근,민승기,오승환,김태성 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.3

This clinical study was made of 214 cases of the jaw cysts which were diagnosed histopathologically in the Department of Oral and Maxillofacial Surgery, Wonkwang University Dental Hospital from Jan. 1, 1991 to Dec. 31, 1998. Medical records, radiographs, histopathological reports of 214 cases with jaw cysts were retrospectively reviewed. The results were as follows : 1.Among the total patient of 214 cases, male were 143 cases, and female are 71 cases, male predominated by the ratio of 2.04. 2.The periapical cysts and the dentigerous cyst are the most common cyst, irrespective of 116 cases(54.2%) and 58 cases(27.1%). 3.The periapical cyst has no gender prediction, male were 70 cases and female were 54 cases, the mean ages were 39.3 years(SD=18.4). The most common involving teeth were maxillary anterior teeth(59.7%) . 4.The dentigerous cyst predominantly occurred in third decade (18 cases : 31%), and the mean ages were 31.9 years(SD= 14.8). Dentigerous cysts predominantly occurred in male by the ratio of 7.29 (male : 51 cases, female : 7 cases). Maxillary anterior teeth(43.1%) and mandibular molars(32.8%) were most frequently involved. 5.The odontogenic keratocyst predominantly occurred in second and third decade and mean ages were 23.5 years(SD= 17.2). The mandibular molars(60%) were most frequently involved. 6.All nasopalatine cyst occurred after fourth decade, mean ages were 49.5years(SD= 10.4). In the gender incidence of nasopalatine cyst, 1 case occurred in female and 10 cases occurred in male. 7.In the surgical intervention of jaw cyst, cyst enucleation and marsupialization performed in 156 cases. Cyst enucleation with bone graft performed in 58 cases and iliac autogenous bone grafts most frequently performed in 29cases. 8.The diameter range of cyst enucleation with bone graft were from 15mm to 120mm with a cyst diameter of 33.3mm. (SD = 15.2). The diameter range of cyst enucleation and marsupialization were from 7mm to 82mm with a cyst diameter of 20.4mm.(SD=9.0) The diameter of cyst enucleation with bone graft were significantly greater than diameter of cyst enucleation. (p<0.05) 9.Post-operative complications occurred in 9 cases(15.5%) in which cyst enucleation with bone grafts performed and occurred in 31 cases(19.9% ) in which cyst enucleation.

불멸화된 악성구상상피에 대한 Retinoic Acid의 세포활성 및 유전자 발현 연구

문철,민승기 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.1

cDNA microarray allows to monitor the expression of thousthand genes simultaneously and has been successfully to explore the gene expression of carcinoma and other diseases. To identify and monitor gene expression profile changes in oral tumorigenesis and exposure to medicine may shed light not only on the cause of these pathological changes, but also provide the opportunity to identify novel targets for disease detection and intervention. Retinoids and interferon are important regulators of human epithelial cell differentiation and have been used successfully in the clinical treatment of HPV-involved cervical cancer. However, there is only a limited information available concerning to the effect or role of retinoic acid in oral precancerous and cancer cells. We studied the effect of retinoic acid on normal human oral keratinocyte(NHOK), HPV-16 E6E7 immortalized human oral keratinocyte(IHOK), and two oral squamous cell line(HNSCC30, HNSCC31) according to the carcinogenesis stage. The RA effect was evaluated by MTT assay and microarray. Complementary DNA microarray including 400 genes was used to systematically characterize the variation in gene expression patterns of RA treated IHOK cells vs. control IHOK cells . The results were as follows: 1. RA has negative effect on growth for all NHOK, IHOK, HNSCC30 and HNSCC31 dependently on the concentration and cultivation time. 2. We identified 18 clones cDNA exhibiting more than 2 fold overexpression in RA treated IHOK probes relative to control probe, 21 cDNA s reveal more than 2 fold overexpression in control IHOK relating to the experimental cells. Pearson correlation coefficients for the set of selected spots from duplicated hybridization was 0.86. 3. Examination of gene expression that differs between RA treated IHOK and control IHOK cells apprear to be related to : cell cycle regulator, cell growth and apoptosis, cell adhesion, signal transduction and miscellanous. 4. Cell cycle regulator uprgulation is noted in MAD2, CDK9, Cdc20, Cdc2, CDK inhibitor 1A, and only HUS-1 is downregulated. 5. Cytochrome P450 and interleukin 1-alpha for cell growth and apoptosis factor was upregualted, and capase 8 & FADD apotosis regualtor gene was decreased expression, so we observed increased apoptosis mecahnism and resistance to apoptosis. 6. Cytokine upregulation in RA treated IHOK was noted in interIeukin 1 alpha, downregualtion was in interleukin 8, interleukin 10, interleukin 10 receptor, interleukin 3 receptor, interleukin 9 receptor, and interleukin 19 7. Signal transducers including JAK binding protein, STAT1, and Burkitt lymphoma receptor were upregulated with RA reated IHOK , suggesting the RA regulates the JAK/STAT signal-transduction pathway. In conclusion, we have demonstrated the high-throughput utility of cDNA array hybridization in parallel to the gene expression analysis to identify genes that are expressed differentially in RA treated with HPV16 immortalized keratinocytes. The differentially expressed genes identified here should be informative in oral epithelial cell carcinogenesis.

飼料作物의 生育에 關한 調査 Ⅱ : 晉州地方을 中心으로 for Chinju Area

文勝式,金基元,鄭鉉承 진주산업대학교 1971 論文集 Vol.8 No.-

Sowing 6 Kinds of leguminous grasses 8 Kinds of forage grasses 6a ward of mixture grasses 20 kinds in total, I had bbserved them for 8 month (Marchs. 1970~ october 30 1970) and acquired the results as follow; 1. The growth of forage crops and mixture was well in good soil with plenty rainfall in moderate atmospherical condition. 2. The summer drought is sure in ladino clover alfalfa, birds foot trefoil, Perennial ryegrass, Timothy and Brome grass, The mixture ward is comparatively little. 3. The respective fresh yield of orchard grass, Red clover O+OL+B+R were 5,500㎏, 5,100㎏, 4,325㎏.

무선 애드 혹 기반 액티브네트워크상에서 QoS지원에 관한 연구

李基源,裵振勝,丁燦赫,金玄郁,李光培,姜景仁,朴京培,文泰洙,郭承郁,河載承 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - In this thesis, we propose a QoS supporting method on wireless ad hoc-based active network for improving reliability. On the loss of route path, the existing DSR routing protocol has several problems with wireless network communication because of decease of data reception rate and increase of network load. The proposed method improves reliability of data transfer by using functions of active network. That is, by using functions of active nodes and active packets, the proposed method supports QoS with reservation transfer method that saves data packets on transfer at intermediate nodes with lost path and then re-transmit the packets after route reconfiguration.

Inconel 718 상향 엔드밀링시 절삭력에 미치는 공구형상오차의 영향

이영문,양승한,장승일,백승기,김선일,이동식 한국공작기계학회 2002 한국생산제조학회지 Vol.11 No.5

In an end milling process, the undeformed chip section and cutting forces vary periodically with the phase change of the tool. However, the real undeformed chip section area deviates from the geometrically ideal one owing to the cutter runout and tool shape error. In the current study, a method of estimating the real undeformed chip section area which reflects the cutter runout and tool shape error is presented during up-end milling processes of Inconel 718. The specific cutting forces, K_r and K_t are defined as the radial and tangential cutting forces divided by the modified chip section area, respectively. Both of the K_r and K_t values become smaller as the helix angle increases from 30˚ to 40˚. Whereas they become larger as the helix angle increases from 40˚ to 50˚. The K_r and K_t values show a tendency to decrease with increase of the modified chip section area.

하악 제3대구치가 하악 우각부골절 정복술후 감염에 미치는 영향에 관한 연구

최문기,민승기,이동근,오승환 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

Any fracture passing through the socket of a teeth is compounded intraorally, even if the fracture is not displaced and the tooth is firm in its socket. Before the advent of antibiotic therapy the danger of infection in a compounded fracture posed severe problems in treatment. Infection is reduced by antibiotic therapy but prolonged use of antibiotics is not justified in an attempt to save a tooth which might eventually be sacrificed. There is still controversy in the management of third molar in mandibualr angle fracture, particularly in regard to their retention or removal at the time of fracture treatment. So we surveyed the 159 patients who were treated with open reduction of mandibular angle fracture containing third molar in fracture line, and compared with the postoperative infection rate depending on time intervals between injury and operation, eruption state of third molar, non-extraction or extraction of third molar related to eruption state, non-extraction or extraction of third molar related to condiition of third molar and its surrounding periodontium and were to propose treatment guidline of third molar in mandibular angle fracture The results obtained were as follows : 1.There were no statistical significance between the time from injury to operation and postoperative infection. 2.There were no statistical significance between eruption state of third molar and postoperative infection. 3.In case of retention of the third molar, there were no statistical significance between eruption state of third molar and postoperative infection, but in case of extraction, postoperative infection was high rate in complete impacted cases. 4.There were no statistical significance between non-extraction or extraction of third molar and postoperative infection depending on condition of third molar. There are no difference in infection rate statistically according to the time from injury to operation, eruption state and condition of third molar, but retention of third molar revealed lowered infection rate in completely impacted cases. By terms of the manegement of third molar, we should extract or preserve third molar in the line of the mandibular angle fracture according to possibility of infection.

Intermediate Driver 기반의 개인 방화벽 구현

최승문,김기철,이정문 강원대학교 정보통신연구소 2002 정보통신논문지 Vol.6 No.-

In this paper, a new technique is proposed for the implementation of personal firewall using the intermediate driver. It meets the Network Driver Interface Specification(NDIS) and Windows Driver Model(WDM) standards. A prototype personal firewall based on this technique shows good performance in packet filtering according to the ruleset against possible attempts of hacking.

당뇨병환자에서 뇨증 Fibronectin농도의 증가에 관한 연구

한승범,조준승,손건영,서성문,박근용,조성래,박규영,박정모,이인규,여준기 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Plasma fibronectin is an α₂-glyoprotein, which is produced by vascular endothelial cells. Raised level of plasma fibronectin has been observed in diabetic patients particularly in the presence of microvascular complications. However, no available data exist about urine level of this glycoprotein in diabetic patients. We measured urine fibronectin level by ELISA methods in 54 diabetic patients who have microvascular comlications or not. The following results were obtained. 1) Urine fibronectin level(㎍/g creatinine) in diabetic group(1740.0±678.0) is increased compaired with those in normal control group(471.0±59.0). but this results were not significantly different among two groups(P>0.05). 2) Urine fibronectin level(㎍/g creatinine) was significantly increased in patients with nephropathy (6188.0±3144.0) compared with those in normal control group(471.0±59.0) and patients without nephropathy(645.0±251.0)(P<0.001). 3) There were a significant correlation between BUN, creatinine, creatinine clearance, 24hr urine total protein and urine fibronectin level in diabetic patients. Our data suggest that urine fibronectin excretion level might be used as a sensitive guide for diabectic nephropathy.