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- 저자 : ( Seon Mi Yu ) (검색결과 374 건)
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활성 슬러진에서 분리한 세균 Delftia sp. JK-2에 의한 산업 유기용매의 제거
황선영,유미옥,오계헌 순천향대학교 기초과학연구소 2003 순천향자연과학연구 논문집 Vol.9 No.2
Activated sludge samples were collected from a municipal sewage treatment plant and used for enrichment of microbial consortia with aniline as the sole carbon and nitrogen source. A bacterium which had excellent aniline degradability was isolated for this work. Biodegradation of industrial organic solvents such as aniline, benzoate, or p-hydroxybenzoate by Delftia sp. JK-2 was examined in the media containing the target substrates in Erlenmeyer flasks, respectively. The JK-2 cells degraded 10 mM aniline, 5 mM benzoate or 5 mM p-hydroxybenzoate completely within 36 hours, 48 hours or 40 hours, respectively. Microscopic examination of the strain revealed Gram-negative and short rod cells. Physiological analysis using BIOLOG GN2 MicroPlate™ system was performed to identify the strain JK-2. As the results of this identification processes, the strain JK-2 could be assigned to genus Delftia, and designated as Delftia sp. JK-2
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김명선,손미란,전진호,유병철 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2
Background : This study purposed to propose the fundamental data to develop the proper health promotion program through observation about the current status, lifestyle behaviors and results of health examinations of public personnel in Busan Metropolitan City. Methods : Subjects were 988 public services (683 male, 305 female) who were employed in City Hall of Busan Metropolitan City. We investigated the relation between lifestyle behaviors and self recognized health status using health examination in 2006. Data analysis on multiple logistic regression and multi-dimensional scaling were done using SPSS win(ver 12.0k) program. Results : The proportion of above 50 years old age are 47.1% in male and 59.7% in female. There are 10.2% in male and 15.8% in female with family history of hypertension, and 8.5% in male and 14.5% in female with family history of diabetes mellitus. There are 37.7% in male and 12.1% in female with obesity, and 10.6% in male and 7.7% in female with abnormal liver function. The disease suspicion rate for male was 1.8%, and 4.6% for female. Risk of hypertension in male was 3.7 times greater than in female and risk of diabetes mellitus in males was 5.0 times greater than in female. By questionnaire 71.8% in male and 78.3% in female had been thought themselves to have disease. Both male and female participants were more likely to think themselves with disease according to disease history of diabetes mellitus, liver dysfunction and hypercholesterolemia. Also aging is interfered that self-recognized health status. Conclusion : Self recognized health status was associated with diabetes mellitus, liver dysfunction, hypercholesterolemia, family history of chronic disease and aging in public employee of Busan Metropolitan City. This association point that there is need for continuous education and effort to modify their life style.
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Yu, Seon-Mi,Kim, Hyun-Ah,Kim, Song-Ja Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.7
2-deoxy-D-glucose (2DG) is known as a synthetic inhibitor of glucose. 2DG regulates various cellular responses including proliferation, apoptosis and differentiation by regulation of glucose metabolism in cancer cells. However, the effects of 2DG in normal cells, including chondrocytes, are not clear yet. We examined the effects of 2DG on dedifferentiation with a focus on the ${\beta}$-catenin pathway in rabbit articular chondrocytes. The rabbit articular chondrocytes were treated with 5 mM 2DG for the indicated time periods or with various concentrations of 2DG for 24 h, and the expression of type II collagen, c-jun and ${\beta}$-catenin was determined by Western blot, RT-PCR, immunofluorescence staining and immunohistochemical staining and reduction of sulfated proteoglycan synthesis detected by Alcain blue staining. Luciferase assay using a TCF (T cell factor)/LEF (lymphoid enhancer factor) reporter construct was used to demonstrate the transcriptional activity of ${\beta}$-catenin. We found that 2DG treatment caused a decrease of type II collagen expression. 2DG induced dedifferentiation was dependent on activation of ${\beta}$-catenin, as the 2DG stimulated accumulation of ${\beta}$-catenin, which is characterized by translocation of ${\beta}$-catenin into the nucleus determined by immunofluorescence staining and luciferase assay. Inhibition of ${\beta}$-catenin degradation by inhibition of glycogen synthase kinase 3-${\beta}$ with lithium chloride (LiCl) or inhibition of proteasome with z-Leu-Leu-Leu-CHO (MG132) accelerated the decrease of type II collagen expression in the chondrocytes. 2DG regulated the post-translational level of ${\beta}$-catenin whereas the transcriptional level of ${\beta}$-catenin was not altered. These results collectively showed that 2DG regulates dedifferentiation via ${\beta}$-catenin pathway in rabbit articular chondrocytes.
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YU, SEON-MI,KIM, SONG-JA UNKNOWN 2015 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.35 No.2
<P>Dedifferentiation and inflammation are major features of cartilage degeneration during the pathogenesis of osteoarthritis (OA). Thymoquinone (TQ) is the major compound of black seed oil isolated from <I>Nigella sativa</I> with various beneficial or harmful effects on several diseases; however, its effects on the dedifferentiation and inflammation of chondrocytes have not yet been characterized. In the present study, we investigated whether TQ regulates the dedifferentiation and inflammation of rabbit articular chondrocytes, focusing on the production of reactive oxygen species (ROS) in rabbit articular chondrocytes. TQ induced the generation of ROS in a dose-dependent manner, as shown by staining with the fluorescent probe, 2′–7′-dichlorofluorescein diacetate. We confirmed that TQ induced dedifferentiation by measuring the loss of type II collagen and the reduction in chondroitin sulfate proteoglycan levels. TQ also caused inflammation by inducing the expression of cyclooxygenase-2 (COX-2) and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>). The antioxidant, N-acetyl cysteine (NAC), prevented the dedifferentiation and inflammation which was generated by the TQ-induced production of ROS. Furthermore, TQ caused a dose-dependent increase in p38, phosphorylated extracellular signal-regulated kinase (p-ERK) and phosphoinositide 3-kinase (PI3K) expression. NAC abrogated this effect and attenuated the dedifferentiation and inflammation which was generated by the TQ-induced production of ROS. To identify the ROS-regulated pathways, we treated the chondrocytes with the p38 inhibitor, SB203580, the MEK inhibitor, PD98059, and the PI3K inhibitor, LY294002. PD98059 inhibited the TQ-induced dedifferentiation and SB203580 and LY294002 prevented the TQ-induced inflammation. These findings suggest that the TQ-induced production of ROS causes dedifferentiation through the ERK pathway and inflammation through the PI3K and p38 pathways in rabbit articular chondrocytes.</P>
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Construction of locust-pathogenic fungal library and use as biological control agents
Mi Rong Lee,Jeong Seon Yu,Min Ho Song,Se Jin Lee,Jae Su Kim 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
ocust, Locusta migratoria (Orthoptera: Acrididae) is one of the outbreaking pests worldwide and such big occurrence was recorded in 2014, Korea, however little consideration was given to the management strategy of the pest. Herein we established a indoor locust-rearing system and constructed a locust-pathogenic fungal library to further facilitate the resources to be used as possible biological control agents. A locust colony was provided from the National Institute of Agricultural Science and Technology and reared in corn or barley plants at artificially manipulated rooms. The critical developmental stages, such as oviposition, hatching and mating were successfully proceeded. Entomopathogenic fungal granules were treated to the locust (2 g/rearing box), and in 5~7 days mycosis was observed in the membranous cuticles of head, abdomen and legs. In particular JEF-003 (Metarhizium anisopliae), JEF-186 (M. lepidiotae) and JEF-187 (Clonostachys rogersoniana) showed high virulence against the locust. A population of locust was exposed to the entomopathogenic fungal conidia-incorporated soil to investigate the possibility of the fungal isolation from natural soil, which resulted in the pathogenesis in 7~10 days in laboratory conditions. More than 80% of control efficacy was observed in the greenhouse trial of fungal granular application. This work suggests that locust rearing system was successfully established and entomopathogenic fungi can be used to control the migratory locust.
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Yu, Seon-Mi,Cho, Hongsik,Kim, Gwang-Hoon,Chung, Ki-Wha,Seo, Sung-Yum,Kim, Song-Ja SAGE Publications 2016 Experimental biology and medicine Vol.241 No.8
<P>Osteoarthritis is a nonrheumatologic joint disease characterized by progressive degeneration of the cartilage extracellular matrix. Berberine (BBR) is an isoquinoline alkaloid used in traditional Chinese medicine, the majority of which is extracted from Huang Lian (Coptis chinensis). Although numerous studies have revealed the anticancer activity of BBR, its effects on normal cells, such as chondrocytes, and the molecular mechanisms underlying its actions remain elusive. Therefore, we examined the effects of BBR on rabbit articular chondrocytes, and the underlying molecular mechanisms, focusing on actin cytoskeletal reorganization. BBR induced dedifferentiation by inhibiting activation of phosphoinositide-3(PI3)-kinase/Akt and p38 kinase. Furthermore, inhibition of p38 kinase and PI3-kinase/Akt with SB203580 and LY294002, respectively, accelerated the BBR-induced dedifferentiation. BBR also caused actin cytoskeletal architecture reorganization and, therefore, we investigated if these effects were involved in the dedifferentiation. Disruption of the actin cytoskeleton by cytochalasin D reversed the BBR-induced dedifferentiation by activating PI3-kinase/Akt and p38 kinase. In contrast, the induction of actin filament aggregation by jasplakinolide accelerated the BBR-induced dedifferentiation via PI3-kinase/Akt inhibition and p38 kinase activation. Taken together, these data suggest that BBR strongly induces dedifferentiation, and actin cytoskeletal reorganization is a crucial requirement for this effect. Furthermore, the dedifferentiation activity of BBR appears to be mediated via PI3-kinase/Akt and p38 kinase pathways in rabbit articular chondrocytes.</P>
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Simvastatin induces differentiation in rabbit articular chondrocytes via Wnt/β-catenin pathway
Yu, Seon-Mi,Han, Yohan,Kim, Song Ja North-Holland 2019 European journal of pharmacology Vol.863 No.-
<P><B>Abstract</B></P> <P>Simvastatin is widely used as a specific inhibitor of 3-hydroxy-3-methyl-glutaryl-CoA reductase to reduce the levels of low-density lipoprotein cholesterol. However, its regulatory mechanism in chondrocyte differentiation is unclear. This study was conducted to evaluate the effects and signalling pathway of simvastatin on chondrocyte differentiation. We found that simvastatin induced chondrocyte differentiation, as confirmed by increased type II collagen expression and induced sulphated proteoglycan synthesis. Western blotting results showed that expression of type II collagen increased 6-fold in a dose-dependent manner compared with that in the control. Further, nuclear/cytosol fraction analysis revealed that simvastatin reduced the expression and translocation of β-catenin into the nucleus from the cytoplasm by approximately 50% compared with that in the control. A luciferase assay using a T cell factor/lymphoid enhancer factor reporter construct was performed to test the transcriptional activity of β-catenin. Simvastatin-induced differentiation was dependent on inactivation of β-catenin, as simvastatin inhibited accumulation of β-catenin, which was characterized by translocation of β-catenin to the nucleus as shown by immunofluorescence staining and the luciferase assay. Prevention of β-catenin degradation by inhibition of the proteasome with z-Leu-Leu-Leu-CHO blocked the increase in type II collagen expression. Simvastatin treatment reduced chondrocyte dedifferentiation induced by retinoic acid or serial monolayer culturing by 50% compared to that in the non-treated cells. Our findings demonstrate that simvastatin increases differentiation of rabbit articular chondrocytes via the β-catenin pathway.</P>
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Yu, Seon-Mi,Kim, Song Ja Elsevier 2018 Biochemical and biophysical research communication Vol.501 No.3
<P><B>Abstract</B></P> <P>Simvastatin is a cholesterol-lowing reagent that is derived synthetically from the fermentation of Aspergillus terreus. Recently, SVT has been shown to possess a protective effect of chondrocytes.</P> <P>Kruppel-like factor 4 (KLF-4) is a zinc finger transcription factor that plays crucial roles during the development and maintenance of multiple organs. However, the roles of KLF-4 in chondrocytes have not been well unknown. Here, we investigated whether KLF-4 regulates SVT-caused differentiated phenotype of chondrocytes. A KLF-4 cDNA or KLF-4 siRNA was transfected into SVT-treated chondrocytes. Western blot analysis, RT-PCR and immunofluorescence staining analyzed expression of type II collagen and SOX-9, marker proteins of differentiation. The results showed overexpression of KLF-4 accelerates SVT-induced type II collagen expression, as determined by western blot analysis and causes sulfated-proteoglycan synthesis, as detected by Alcian blue staining. RT-PCR revealed that ectopic expression of KLF-4 induces SVT-caused SOX-9, a transcription factor of type II collagen, expression. Transfection of KLF-4 siRNA reversed SVT-caused type II collagen and SOX-9 expression and inhibited SVT-induced sulfated proteoglycan production. This study indicates that KLF-4 plays critical role in SVT-caused chondrocytes differentiation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SVT was shown to exhibit a protective effect on chondrocytes. </LI> <LI> KLF-4 regulates the SVT-mediated differentiation of chondrocytes. </LI> <LI> KLF-4 plays critical role in SVT-mediated chondrocyte differentiation. </LI> </UL> </P>
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