지르코니아 clwP 세라믹의 착색 처리조건이 색조변화에 미치는 영향 : Effect of Treatment Condition on Coloring of Zirconia Dental Ceramics

오선미, 이채현 배재대학교 공학연구소 2011 공학논문집 Vol.13 No.1

The Effect of treatment condition on the coloring of zirconia dental ceramics were studied. Four liquid coloring agents and different dipping time, and different sintering condition were chosen. Variety of colors from Al to C4 according to shade guide, were observed by spectrophotometer. Therefore it can be concluded that the color of zirconia core can be controlled by controlling treatment condition such as dipping time, sintering temperature, sintering time, coloring agent, and so on.

Streptococcus oralis 의 인공치태 억제효과에 대한 연구

오종석,정성수,양규호,김선미 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.1

이 연구는 치아우식증이 주요 원인균인 Streptococcus mutans의 치태형성과 증식에 대한 Streptococcus oralis의 영향을 조사하고자 하였다. 이를 위해 S.mutans와 S.oralis 혼합 배양후 형성된 치태 무게와 생균수를 측정비교하였다. 또한 치태형성과 생균수에 영향을 미치는 요소들에 대해 조사하여 다음과 같은 결과를 얻었다. 1. S.mutans와 S.oralis의 혼합배양시 S.mutans 단독배양에 비해 치태무게와 생균수가 감소되었다. 2. S.mutans 단독배양에 10 mM의 표도당을 첨가한 군은 첨가하지 않은 군보다 치태무게가 증가되었으나,S.mutans와 S.oralis의 혼합배양에 10 mM의 포도당을 첨가한 군은 첨가하지 않은 군과 차이가 없었다. 3. S.mutans 단독배양에 10 mM S.mutans와 S.oralis 10 mM의 과당을 첨가한 군도 첨가하지 않은 군보다 치태무게가 증가되었다. 4. S.mutans와 S.oralis의 혼합농도를 달리하여 배양했을때 S.oralis의 혼합농도가 높아질수록 치태무게와 생균수는 감소되었다. 5. 배양기산(6,9,12,15시간)에 따른 치태무게와 생균수는 S.mutans단독배양에 비해 S.mutans와 S.oralis혼합배양시 배양 12시간 이후 급격히 감소되었다. 6. S.mutans와 S.oralis의 혼합배양에 H₂O₂를 소비하는 Staphylococcus epidermidis를 첨가한 군은 첨가히자 않은 군보다 치태무게와 생균수가 증가되었다. 이상의 결과로 S.oralis는 S.mutans와 혼합배양시 S.mutans의 증식과 인공치태의 생성을 억제함을 알 수 있었고, 이는 S.oralis가 H₂O₂를 분비함으로써 얻어진 결과임이 시사되었다. This study was performed to evaluate the effect of Streptococcus oralis on the formation of artificial plaque and the replication of Streptococcus mutans.S.mutans was incubated alone and in the combination with S.oralis in the beaker with wires. The produced plaque weight and the viable cells of S.mutans were compared between those cultures. Various factors were studied about the effect on the formation of plaque and the replication of S.mutans. Followings are the results. 1. Lower amount of plaque was produced and fewer cells of S. mutans were replicated at the mixed culture of S. mutans and S. oralis than S. mutans alone. 2. When 10 mM glucose was added, the plaque weight was increased in the culture of S. mutans alone. But in the mixed culture of S. mutans and S. oralis, the plaque weight was not increased when 10mM of glucose was added. 3. When 10 mM fructose was added, the plaque weight was increased in culture of S. mutans alone or combined S. mutans and S. oralis. 4. In the mixed culture of S.mutans and S. oralis with different concentration, the more S.oralis exist, the less plaque and the fewer viable cells of S. mutans were observed. 5. The plaque weight and the viable cells of S.mutans were more decreased in the mixed culture of S. mutans and S. oralis than S. mutans alone after 12 hours. 6. When Staphylococcus epidermidis consuming hydrogen peroxide was added to the mixed culture of S. mutans and S. oralis, the plaque weight and the viable cells of S. mutans were increased. These results indicate that S. oralis inhibited the formation of plaque and the replication of S. mutans,and this may result from the formation of hydrogen peroxide by S. oralis.

補助動詞「おく」とそれに對應 する韓國語

吳美善 단국대학교 단국일본연구학회 1998 일본의언어와문학 Vol.3 No.-

다운증후군 환자의 치주질환 원인균의 출현율

김선미,양규호,최남기,오종석,강미선 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.4

다운증후군 환자는 치주질환의 진행이 빠르고 치주조직의 파괴가 심하다. 다운증후군 환자의 치주질환 원인균의 출현율을 알아보고자 7~19세 다운증후군 환자 27명과 대조군으로 나이가 비슷한 정신지체자 27명을 대상으로 치태지수와 치은염 정도를 나타내는 치은지수를 측정하고 치은연하 치태에 존재하는 P. gingivalis, T. forsythia, T. denticola, F. nucleatum, A. actinomycetemcomitans균을 중합효소연쇄반응을 이용, 검사하고 다음과 같은 결론을 얻었다. 1. 다운증후군 환자와 대조군의 치태지수와 치은지수간에 통계적 유의성은 없었다(p>0.05). 2. 다운증후군 환자에서 F. nucleatum의 출현율이 96.3%로 가장 높았으며 그 다음이 T. forsythia 74.1%, P. gingivalis는 63.0%였으며 A. actinomycetemcomitans는 55.6%, T. denticola는 40.7%로 출현하였다. 정신지체자는 다운증후군 환자와 비슷한 순서로 치주질환 원인균이 출현하였다. 다운증후군 환자가 대조군에 비해 T. denticola를 제외한 4종의 균에서 놓은 비율로 나타났으나 치주질환 원인균 출현율에서 유의한 차이를 나타내지 않았다(p>0.05). 3. 연령에 따른 연구에서 7~10세군에서 다운증후군환자의 P. gingivalis는 16.7%로 낮은 출현율을 보였으나 연령의 증가에 따라 출현율이 평균 63.0%로 높아졌다. 반면 A. actinomycetemcomitans균은 7~10세군부터 83.3%로 높은 비율을 보였다(p<0.05). 같은 연령대에서 다운증후군 환자는 대조군에 비해 치주질환 원인균의 출현율이 더 높은 경향을 보였으나 A. actinomycetemcomitans를 제외하고는 통계적인 유의차를 보이지 않았다(p>0.05). 4. P. gingivalis, T. forsythia, T. denticola가 함께 나타난 경우는 다운증후군 환자와 대조군 모두 33%를 나타내었다. red complex에 A. actinomycetemcomitans까지가 나타난 경우는 다운증후군 환자에서 22%, 대조군에서 14%로 다운증후군 환자에서 더 높았다. 이상의 결과를 요약해보면 다운증후군 환자군과 정신지체자 대조군 모두에서 치주질환 원인균이 어린 시기부터 매우 높게 출현하였으나 전체적인 두 군간 치태지수, 치은지수, 치주질환 원인균 출현율에서 유의한 차이를 나타내지 않았다. 하지만 A. actinomycetem comitans균은 7~10세군 다운증후군 환자에서 대조군에 비해 높은 비율을 보였다. It is widely known that individuals with Down's syndrome(DS) often develop early onset severe periodontal diseases. In this study. We examined the prevalence of periodontopathic bacteria in DS patients to compare controls with mental disabilities(MD). The subjects were 27 DS patients (7 to 19 years old) and 27 age-matched controls with MD. Plaque index and gingival index were measured. And 5 pathogens, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, were surveyed in subgingival plaque samples using a polymerase chain reaction. No significant difference in plaque index and gingival index were observed between the DS and control group. The prevalence in DS was 96.3% for F. nucleatum, 74.1% for T. forsythia, 63.0% for P. gingivalis, 55.6% for A. actinomycetemcomitans, 40.7% for T. denticola. No significant differences were observed in the prevalence of periodontopathic bacterias between the DS and control. Prevalence of P.g(16.7) at age 7~10 is lower than other age group in DS, but its prevalence increased with age. Prevalence of A.a(83.3%) is peak at age 7~10 in DS. These results suggest that various periodontopathic pathogens can colo nize in the very early childhood of DS and MD patients. But no significant difference was observed in the prevalence of periodontopathic bacterias between the DS and control.

Real-time PCR을 이용한 치주질환 원인균의 정량적 분석

김선미,양규호,최남기,강미선,오종석 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.3

Real-time PCR 기법을 이용하여 구강내 치태에서 특정 세균의 존재여부를 알 수 있을 뿐 아니라 정량적 분석이 가능하게 되었다. 이 연구는 real-time PCR 기법을 이용하여 8-18세의 청소년에서 치주질환 원인균의 조성을 알아보고자 전신질환이 없는 어린이 및 청소년 65명을 대상으로 치은연하의 치태를 채취하여 5종의 치주질환 원인균의 출현율과 그 양을 측정하고,또한 함께 측정한 치태지수 및 치은지수와의 상관관계를 조사하여 다음과 같은 결론을 얻었다. 1. 치태지수의 평균은 1.33이었으며,치은지수의 평균은 0.97이었다. 치태지수는 나이가 증가할수록 낮아졌다(p<0.05). 2. 치주질환 원인균의 출현율은 P. gingivalis 61.5%, T. forsythia 53.8%, T. denticola 29.2%, A. actinomycetemcomitans 15.4%. F. nucleatum은 100%였다. P. gingivalis, T. denticola는 10세 이후 출현율이 증가하였고 A. actinomycetemcomitans는 8-10세 군에서 출현율이 높았다(p<0.05). 3. 정량적 분석에서 치태지수와 치은지수는 F. mucleatum의 양과 유의한 상관성이 있었으며,치태지수는 치은지수와 유의한 상관성이 있었다. T. forsythia는 A. actinomycetemcomitans, T. denticola양과 강한 상관성을 보였으며, T. denticola양과 A. actinomycetemcomitans도 강한 상관관계를 가지고 있었다. The detection of special bacteria of oral plaque and quantitative analysis are possible through the real-time PCR technique. The aim of this study was to investigate the prevalence and the amount of five periodontopathic bacteria using real-time PCR, and evaluate the relationship among amount of bacteria, plaque index and gingival index. Sixty five subjects ranging from 8 to 18 years old were enrolled. Plaque index and gingival index were examined and five pehodontopathic bacteria were surveyed in subgingival plaque samples. The results were analyzed by ANOVA and Pearson's correlation. Results: The prevalence of P. gingivalis was 61.5%, T. forsythia was 53.8%, T. denticola was 29.2%, A.actinomycetemcomitans was 15.4% and F. nucleatum was 100%. Plaque index and gingival index have a significant relationship with the amount of F. nucleatum. The amount of T,forsythia has a strong relationship with the amount of T, denticola and A, actinomycetemcomitans(p<.0.05).

활성 슬러진에서 분리한 세균 Delftia sp. JK-2에 의한 산업 유기용매의 제거

황선영,유미옥,오계헌 순천향대학교 기초과학연구소 2003 순천향자연과학연구 논문집 Vol.9 No.2

Activated sludge samples were collected from a municipal sewage treatment plant and used for enrichment of microbial consortia with aniline as the sole carbon and nitrogen source. A bacterium which had excellent aniline degradability was isolated for this work. Biodegradation of industrial organic solvents such as aniline, benzoate, or p-hydroxybenzoate by Delftia sp. JK-2 was examined in the media containing the target substrates in Erlenmeyer flasks, respectively. The JK-2 cells degraded 10 mM aniline, 5 mM benzoate or 5 mM p-hydroxybenzoate completely within 36 hours, 48 hours or 40 hours, respectively. Microscopic examination of the strain revealed Gram-negative and short rod cells. Physiological analysis using BIOLOG GN2 MicroPlate™ system was performed to identify the strain JK-2. As the results of this identification processes, the strain JK-2 could be assigned to genus Delftia, and designated as Delftia sp. JK-2

가정식 음식의 아크릴아마이드 함량분석

이미선,박재영,오상석 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.6

Swedish research in 2002 indicated that acrylamide formation was particularly associated with traditional high temperature cooking processes of certain carbohydrate-rich foods. Since the Swedish report, similar findings have been reported by researchers in numerous other countries. In 2003, three hundred and twenty seven domestic food products, selected on the basis of annual sales in Korea, were analyzed. The results were similar to those from other countries. In this study, the monitoring of acrylamide was expanded to include Korean traditional food products. Samples were purchased from local markets, prepared and then analyzed using LC/MS/MS methods. The categorized food products analyzed included cooked rices and porridges, soup products, fried products, boiled down products, seasoned products, roasted products and coffee. The acrylamide concentrations of the sarnples were ND~18ppb in cooked rices and porridges, ND ~28ppb in soup products, ND~22ppb in fried products, ND~218ppb in boiled down products, <10ppb in seasoned and roasted products, and <10~11ppb in brewed coffee and coffee drinks.

다운증후군 환자의 치아우식증과 치아우식 원인균 및 타액내 면역항체의 연관성

김선미,양규호,최남기,강미선,임회순,오종석 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

다운증후군 환자의 치아우식증과 구강내 여러 요인들과의 연관성을 평가하고자 9-18세의 다운증후군 환자 25명과 정상인 63명을 대상으로 치아우식경험지수, 치태지수를 조사하고 효소면역분석법을 이용하여 타액의 면역항체 IgA와 뮤탄스 연쇄상구균-특이 IgA 농도를 측정하고, Dentocult 키트를 이용하여 뮤탄스 연쇄상구균과 유산간균의 수, 타액의 완충능을 측정하여 두 군의 각 값들을 독립 t-test와 카이제곱 검정을 시행하였다. 다운증후군 환자에서 유치우식경험은 정상인에 비해 유의하게 낮았으나(p<0.001), 영구치우식경험은 유의한 차이를 보이지 않았다. 치태지수와 타액의 IgA의 농도는 두 군 간에 차이를 보이지 않았으며, 뮤탄스 연쇄상구균 특이-IgA 농도와 타액 완충능은 다운증후군환자에서 낮았다(p<0.05), 뮤탄스 연쇄상구균 수와 유산간균 수는 두 군간에 유의한 차이는 없었다. 9-11세 군에서 뮤탄스 연쇄상구균의 수는 다운증후군 환자에서 정상인보다 낮았으며(p<0.001), 뮤탄스 연쇄상구균 특이-IgA는 다운증후군 환자에서 유의하게 낮았다(p<0.05), 유치의 치아우식경험치면지수는 완충능, 뮤탄스 연쇄상구균의 수와 유의한 상관관계를 보였다. This study investigated the relationship between dental caries and various oral factors in subjects with Down' s syndrome. We compared 25 subjects with Down's syndrome with 63 healthy control. The dental caries index and plaque index were examined, and the total salivary immunoglobulin A and S. mutans specific salivary immunoglobulin A concentration were measured using ELISA. The S. mutans counts, Lactobacillus counts and buffer capacity were measured with Dentocult test medium. The decayed and filled surface index of deciduous teeth in subjects with Down' s syndrome was lower than in controls(p<0.001). The plaque index and total salivary immunoglobulin A concentration showed no difference, S. mutans specific salivary immunoglobulin A concentration and buffer capacity in subjects with Down' s syndrome were lower than in controls(p<0.001). There was no significant difference between two groups in the S. mutans counts and Lactobacillus counts. In 9-11 year age group, S. mutans counts in subjects with Down' s syndrome was lower than in controls(p(0.001) and S. mutans specific salivary immunoglobulin A concentration was lower(p(0.05). There was a high correlation among deciduous dental caries index and buffering capacity and S. mutans counts.

치태 형성과 휘발성 유황화합물 생성을 억제하는 Lactobacillus salivarius의 분리 및 동정

김미형,양규호,최남기,김선미,오종석 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.2

우리 인체에는 의학적으로 유용한 기능을 가진 세금들이 정상적으로 존재하며 구강내에도 독특한 기능을 나타내는 세균들이 상재한다. 본 연구에서는 치아우식증이 없는 소아의 타액에서 분리한 유산균 2주가 Streptococcus mutans에 의한 인공치태 형성과 혐기성 세균에 의한 휘발성 유황화합물 생성을 억제하는 것을 화인하고, API 50 CHL medium kit를 이용한 생화학적 검사와 165 rDNA sequencing으로 동정하여 다음과 같은 결과를 얻었다 1. 분리균주는 2주 모두 그람양성 간균으로 과산화수소를 생성하였다. 2. 인공치태의 무게는 Streptococcus 단독 배양시 124.4土30.4mg이었으나, 분리균주와 병합 배양시에는 각각 5.2±2.0mg, 10.6±6.6mg으로 현저하게 감소하였다(p<0.05). 3. Steptococcus mutans의 생균수는 단독 배양시 ml단 3.4×10^(9)이었으나, 분리균주와 병합 배양시에는 각각 ml당 4.6x1과 2.4×10^(8)으로 감소하였다. 4. Fusobacterium nucleatum을 30분간 진탕한 후 측정한 상청액의 흡광도는 1.286이었으나, Fusobacterium nucleatum과 분리균주를 병합으로 30분간 진탕한 후 측정한 상청액의 흡광 도는 각각 0.628과 0.497로 감소하였으며, 상호결합 정도는 29.4%와 57.8%이었다 5. Fusobacterium nucleatum 단독 배양시 cysteine과 FeSO₄를 첨가한 배지를 가한 후 측정한 침전물의 배지 흡광도는 1.794이었으나, 분리균주와 병합 배양시 측정한 침전물의 배지 흡광도는 각각 1.144와 0.915로 감소하였으며, Porphyromonas gingivalis단독 배양시 침전물의 배지 흡광도는 1.932이었으나 분리균주와 병합 배양시에는 침전물의 배지 흡광도가 각각 1 170과 1.266으로 감소하였다. 6. 분리균주를 API 50 CHL medium kit로 탄수화물 발효검사를 시행한 결과, 분리균주 1주는 Lactobacillus salivarius로, 다른 분리균주는 Lactobacillus delbruekii subsp. salicinius로 동정되었다. 7. 분리균주를 165 rDNA partial sequencing으로 동정 한 결과, 2주 모두 Lactobacillus salivarius subsp. sailcinius와 유전자 유사치가 99.60% 99.73%를 보여 Lactobacillus salivarius subsp. salicinius로 동정되었다. 이상의 결과를 종합하면 치아우식증이 없는 소아의 타액에서 분리된 유산균 중 과산화수소를 분비하여 인공치태 형성과 휘발성 유황화합물 생성을 억제하는 분리균주는 Lactobacillus salivarius subsp. Salicinius로 동정되었다. There are normal inhabitants doing medically useful functions in the body. There are many kinds of bacteria performing specific functions in the oral cavity. Two strains of lactic acid bacteria were isolated from inhabitants of caries-free children's oral cavity, which inhibited the formation of artificial plaque by Streptococcus mutans and the production of volatile sulfur compounds by anaerobic bacteria. The isolates were identified by the test using API 50 CHL medium kit and 16S rDNA partial sequencing. 1. Two isolates were Gram-positive bacilli and produced hydrogen peroxide. 2. When Streptococcus mutans was cultured in the media, the mean weight of formed artificial plaque on the orthodontic wires was 124.4±30.4 mg. whereas being reduced to 5.2±2.0mg and 10.6±6.6mg in the media cultured with Streptococcus mutans and each isolate, respectively (pW.05). 3. The number of viable cells of Streptococcus mutans was 3.4 x 10^(9) per ml in the cultured solution, whereas those of Streptococcus mutans in the combined culture with each of isolates were 4.6x 10^(8) and 2.4x 10^(8) per ml. 4. The optical density was 1.286 in the supernatant of Fusobacteriumn nucleatum after vortexing fol 30minutes, whereas in the supernatant of combined Fusobacterium nucleatum and each isolate, they were reduced to 0.628 and 0.497, which the percentages of coaggregation between them were 29.4% and 57.8%, respectively. 5. The optical density of Fusobacterium nucleatum precipitate was 1.794 in the culture media containing cysteine and FeSO4, being reduced to 1.144 and 0.915 in the coaggregated precipitates of Fusobacterium nucleatum and each isolate. The optical density of Porphyromonas gingivalis precipitate was 1,932 in the culture media, being reduced to 1.170 and 1.266 in the coaggregated precipitates of Porphyromonas gingivalis and each isolate, 6. When two isolates were tested with API 50 CHL medium kit, those were identified as Lactobaciallius salivarius and Lactobacillus delbrueckii subsp. lactis. 7. The similarity values of 16S rDNA sequence between each of isolates and Lactobacillus salivarius subsp. salicinius were 99.60% and 99.73%, respectively, meaning that isolates were Lactobacillus salivarius sub-sp. salicinius. These results indicated that two strains isolated from caries-free children's saliva, which inhibited the formation of artificial plaque and the production of volatile sulfur compounds, were identified as Lactobacillus salivarius subsp. salicinius.

Streptococcus oralis의 인공치태 억제효과에 대한 연구

김선미,양규호,정성수,오종석 전남대학교 치과대학 1999 전남치대논문집 Vol.11 No.1

This study was performed to evaluate the effect of Streptococcus oralis on the formation of artificial plaque and the replication of Streptococcus mutans. S. mutans was incubated alone and in the combination with S. oralis in the beaker with wires. The produced plaque weight and the viable cells of S. mutans were compared between those cultures. Various factors were studied about the effect on the formation of plaque and the replication of S. mutans. Followings are the results. 1. Lower amount of plaque was produced and fewer cells of S. mutans were replicated at the mixed culture of S. mutans and S. oralis than S. mutans alone. 2. When 10 mM glucose was added, the plaque weight was increased in the culture of S. mutans alone. But in the mixed culture of S. mutans and S. oralis, the plaque weight was not increased when 10 mM of glucose was added. 3. When 10 mM fructose was added, the plaque weight was increased in the culture of S. mutans alone or combined S. mutans and S. oralis. 4. In the mixed culture of S. mutnas and S. oralis with different concentration, the more S. oralis exist, the less plaque and the fewer viable cells of S. mutans were observed. 5. The plaque weight and the viable cells of S. mutans were more decreased in the mixed culture of S. mutans and S. oralis than S. mutans alone after 12 hours. 6. When Staphylococcus epidermidis consuming hydrogen peroxide was added to the mixed culture of S. mutans and S. oralis, the plaque weight and the viable cells of S. mutans were increased. 7. When 10,000 units of catalase were added to the mixed culture of S. mutans and S. oralis, the plaque weight and the viable cells of S. mutans were increased. 8. In culture of S. mutans, the more concentration of hydrogen peroxide was added, the less plaque and the fewer viable cells of S. mutans were produced. These results indicate that S. oralis inhibited the formation of plaque and the replication of S. mutans, and this may result from the formation of hydrogen peroxide by S. oralis.