A Comparative Experimental Study of the, In-vitro Efficiency of Hypertonic, Saline-Enhanced Hepatic Bipolar and, Monopolar Radiofrequency Ablation

Lee, Jeong Min,Han, Joon Koo,Kim, Se Hyung,Sohn, Kyu Li,Lee, Kyoung Ho,Ah, Su Kyung,Choi, Byung Ihn Korean Radiological Society 2003 Korean Journal of Radiology Vol.4 No.3

Development of multiplexed analysis for the photocatalytic activities of nanoparticles in aqueous suspension

Lee, No Ah,Kim, Soo Jin,Park, Bong-Jae,Park, Hyun Min,Yoon, Minjoong,Chung, Bong Hyun,Song, Nam Woong Royal Society of Chemistry 2011 PHOTOCHEMICAL AND PHOTOBIOLOGICAL SCIENCES Vol.10 No.12

<P>A multiplexed assay technique to measure the photocatalytic activity (PCA) of nanoparticles (NPs) in aqueous suspension was developed based on the observation of TiO<SUB>2</SUB> NPs-photocatalytic oxidation rate of NADH by monitoring the fluorescence intensities. 96 sample solutions of a small volume (<150 μL) could be assayed in a single run without separation of NPs within 15 min. PCA values can be measured with high sensitivity and low experimental uncertainties through the observation at various concentrations of photocatalyst, substrate, aqueous protons and pH buffer ions in a short measurement time.</P> <P>Graphic Abstract</P><P>A multiplexed assay to measure the photocatalytic activity of nanoparticles in aqueous suspension without separation has been developed based on the fluorescence reading in a 96-well plate platform. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1pp05244f'> </P>

O-deGlcNAcylation is required for <i>Entamoeba histolytica</i>-induced HepG2 cell death

Lee, Young Ah,Min, Arim,Shin, Myeong Heon Elsevier 2018 Microbial pathogenesis Vol.123 No.-

<P><B>Abstract</B></P> <P> <I>Entamoeba histolytica</I> is an enteric tissue-invading protozoan parasite that causes amoebic colitis and occasionally liver abscess in humans. <I>E. histolytica</I> can induce host-cell apoptosis by initiating various intracellular signaling mechanisms closely associated with tissue pathogenesis and parasitic immune evasion. O-GlcNAcylation, similar to phosphorylation, is involved in various cell-signaling processes, including apoptosis and proliferation, with O-GlcNAc addition and removal regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), respectively. However, whether O-GlcNAc alterations in host cells affect <I>E. histolytica</I>-induced cell death and which signal molecules participate in <I>E. histolytica</I>-induced deglycosylation remain unknown. In this study, co-incubation of HepG2 cells with <I>E. histolytica</I> increased DNA fragmentation and LDH release as compared with control cells. Additionally, Gal-lectin-mediated amoebic adherence of live trophozoites to HepG2 cells decreased O-GlcNAcylated protein levels within 5 min. We also observed a rapid decrease in cellular OGT protein level, but not OGA, in HepG2 cells in a contact-dependent manner. Furthermore, HepG2 pretreatment with OGA inhibitors or OGA siRNA prevented <I>E. histolytica</I>-induced O-deGlcNAcylation, DNA fragmentation, and LDH release. Our results suggested that <I>E. histolytica</I>-induced O-deGlcNAcylation in HepG2 cells was an important process required for hepatocyte cell death induced by <I>E. histolytica</I> adherence.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Entamoeba histolytica</I> remarkably induces cell death in HepG2 cells. </LI> <LI> O-GlcNAcylated proteins were rapidly and dramatically reduced in Entamoeba-treated HepG2 cells in a contact-dependent manner. </LI> <LI> Alteration of OGT/OGA expression with OGA inhibitor reduces <I>E. histolytica</I>-induced O-deGlcNAcylation in HepG2 cells. </LI> <LI> Inhibition of OGA activity reduces <I>Entamoeba</I>-induced host cell death in HepG2 cells. </LI> </UL> </P>

부추 추출물에 의한 Escherichia coli 및 Staphylococcus aureus 의 생육 저해효과

이민경(Min Kyung Lee),이정아(Jeong-ah Lee),박인식(Inshik Park) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.1

식품오염의 지표로 이용되는 Escherichia coli 와 식중독을 일으키는 Staphylococcus aureus에 대한 부추(Allium tuberosum) 추출물의 항미생물 활성을 검토하고, 그 활성의 열안정성 및 pH 안정성을 조사하였다. 부추 추출물은 미생물의 생육을 저해하였으며 특히 Staphylococcus aureus에서 생육 저해환의 면적이 크게 나타났다. 그리고 paper disc에 50 μL의 부추 추출물을 흡수시켰을 때는 Escherichia coli와 Staphylococcus aureus는 각각 440 ㎟, 609 ㎟의 저해면적을 나타내었으며 부추 추출물의 농도가 높을수록 생육 저해가 현저하였다. 또한 부추 추출물을 68℃에서 30분 또는 98℃ 20분간 열처리 후에도 부추 추출물에 의하여 미생물의 생육은 저해되었다. 그리고 부추 추출물을 pH 2.0에서 3시간 보관 후에도 항미생물활성은 비교적 안정하였다. 그러나 부추 추출물의 항미생물 활성은 투석에 의하여 완전히 활성이 없어졌으며, 따라서 부추 추출물에 존재하는 항미생물 활성은 저분자 물질인 것으로 사료된다. The growth retardation of Escherichia coli and Staphylococcus aureus by heat or acid treated leek (Allium tuberosum) extract was observed. Antimicrobial activity of the leek was the most effective when fresh leek extract was used, but it was stable after heat treatment at 68℃ for 30 min or 98℃ for 20 min. It was also relatively stable after incubated at pH 2.0 for 3 hrs. The antimicrobial activity in leek was not detected after dialysis with molecular weight cutoff of 12,000. Therefore it seems to be small molecule with molecular weight lower than 12,000.

근적외선 유도 약물방출을 위한 키토산 미세입자의 제조와 평가

김민아 ( Min Ah Kim ),김미리 ( Miri Kim ),이창문 ( Chang-moon Lee ) 한국키틴키토산학회 2016 한국키틴키토산학회지 Vol.21 No.4

본 연구에서는 근적외선 레이저의 감응성 물질인 멜라닌과 5-FU를 함유한 키토산 미세입자를 제조하고 광열효과와 약물방출실험을 진행하고 다음과 같은 결과를 얻었다. 멜라닌과 5-FU를 함유한 키토산 미세입자는 W/O/W 방법을 통해 성공적으로 제조할 수 있었고, 미세입자는 구형이었으며 비교적 균일하였다. 키토산 미세입자를 농도별로(0.55~2.75 mg/mL) 증류수에 분산시켜 레이저(808 nm, 1.5 W/cm², 5 min)를 조사한 결과, 샘플의 농도와 조사시간이 증가할수록 온도의 변화가 크게 나타났다. 키토산 미세입자로부터 5-FU의 방출실험을 진행한 결과, 레이저를 조사하지 않은 그룹에 비해 레이저를 조사한 그룹에서 약물방출 속도가 증가하는 것을 알 수 있었고, pH 5.0에서 보다 큰 차이를 보였다. 위의 결과를 통해 근적외선을 이용하여 멜라닌을 함유한 키토산 미세입자로부터 약물 방출 속도를 조절할 수 있고 더 나아가 in vivo에서의 국소적 약물 전달 및 효과적인 치료방법으로 사용될 수 있을 것으로 기대할 수 있다. In this study, melanin and 5-fluorouracil (5-FU)-loaded chitosan microparticles were prepared and the drug release profiles from the microparticles were investigated under near infrared (NIR) 808 nm laser irradiation at 1.5 W/cm ² for 5 min. The chitosan microparticles were uniform and spherical in shape. After irradiation of NIR 808 nm laser at 1.5 W/cm² for 5 min, the temperature of the chitosan microparticle solution increased. As the concentration of the chitosan microparticles increases from 0.55 mg/mL to 2.75 mg/mL, the temperature of the microparticle solution increased with irradiation time. Under NIR 808 nm laser irradiation, 32.1±2.3% and 53.7±1.1% of 5-FU from the chitosan microparticles were released at pH 7.4 and 5.0, respectively. In other hand, without laser irradiation 23.9±1.6% and 26.9±2.0% of 5-FU were released from the chitosan microparticles at pH 7.4 and 5.0, respectively. All these results indicate that chitosan microparticles containing melanin could be used as a photo-induced drug release system.

한국산 무우 Peroxidase의 열변성 및 재활성화에 미치는 요인

이경아(Kyung-Ah Lee),홍정민(Jung-Min Hong),김기남(Gi-Nahm Kim),박인식(Inshik Park) 한국식품영양과학회 1990 한국식품영양과학회지 Vol.19 No.4

한국산 무우(Rhapanus sativus) peroxidase의 열안정성에 미치는 요인 및 열처리에 의해 불활성화된 peroxidase의 재활성화에 미치는 요인들을 검토하였다. 효소는 60℃이하의 열처리에는 안정하였으나, 80℃에서는 10분 후에 완전히 불활성화하였다. 효소의 불활성화에 미치는 PH의 효과 PH 6.0에서는 매우 안정하였으나, PH 4.0이하 및 PH 8.0이상에서는 매우 불안정하였다. 효소의 열안정성은 당, 염 및 단백질의 첨가에 의하여 증가하였다. 효소의 재활성화는 PH 9.0에서 재활성화율이 가장 높았으며, 그리고 열에 의한 불활성화와 다르게 첨가한 당, 염 및 단백질에 의해 거의 영향을 받지 않았다. 열에 의해 불활성화된 효소는 환원제인 dithiothreitol의 첨가에 의하여 재활성화가 억제되었다. Factors affecting thermal inactivation and reactivation of korean radish peroxidase were investigated. The enzyme was stable below 60℃, but it was completely inactivated by heat treatment at 80℃ for 10 min. The enzyme was stable at pH 6.0, but it was unstable below pH 4.0 and above pH 8.0. The thermostablity of the enzyme was increased by addition of glucose, sodium chloride and albumin. The inactivated enzyme by heat treatment was reactivated at room temperature. The optimal pH for reactivation of the enzyme was pH of 9.0. The reactivation rate of the enzyme was not affected by addition of glucose, sodium chloride and albumin. The reactivation was completely inhibited by addition of sulfhydryl reagent such as dithiothreitol.

단신 : 실리콘의 염소화반응에 의한 사염화규소 제조

박균영 ( Kyun Young Park ),이미선 ( Mi Sun Lee ),김민철 ( Min Cheol Kim ),이찬희 ( Chan Hee Lee ),박회경 ( Hoey Kyung Park ),강태원 ( Tae Won Kang ),정해성 ( Hae Seong Jeong ),한경아 ( Kyoung Ah Han ),허원회 ( Weon Hoe Huh ),유지 한국화학공학회 2013 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.51 No.3

직경 25 mm의 파이렉스 튜브 내에서 실리콘의 유동층 염소화 반응이 수행되었다. 반응기에 공급되는 질소 유량0.8~1.0 L/min, 염소 유량 0.2 L/min, 반응온도 450℃, SiCl4 응축기의 냉매온도는 -5℃로 설정하였다. 반응기에 도입되는 가스 내 염소의 몰분율이 증가하면 SiCl4의 수율이 증가하였다. 반응가스 중 염소의 몰분율 0.2의 조건에서 SiCl4의 수율은 28% 이었다. 염소의 몰분율 증가는 반응열 상승에 의해 반응온도 상승을 가져옴으로써 안전을 고려하여 염소의 몰분율을 0.2 이상으로 올리지 못했다. 실리콘의 유동층 염소화 반응에 의한 사염화실리콘의 제조 가능성이 입증되 었으며, 향후 보다 가혹한 조건에서의 실용화 연구를 위한 기초로 활용될 수 있을 것으로 기대된다. The chlorination of a metallurgical-grade silicon was carried out in a fluidized bed reactor, 25 mm in diameter. The flow rate of the chlorine admitted into the reactor was 0.2 L/min and that of the carrier nitrogen was 0.8~1.0 L/ min. The reactor temperature was maintained at 450℃ and the temperature of the coolant at the SiCl4 condenser was at -5℃. The SiCl4 yield increased with increasing the mole fraction of chlorine in the feed gas, exhibiting 28% at the mole fraction of 0.2. Further increase of the chlorine mole fraction was not attempted in a worry that the reactor might be failed due to the high exothermicity of the reaction. The production of SiCl4 from silicon by fluidized bed chlorination was demonstrated on a laboratory scale, which is a stepping stone for future studies under more severe conditions toward industrial application.

Comparisons of obstetrical outcomes among vaginal, intramuscular progesterone treatment and conservative management for the prevention of preterm birth in twin pregnancies with a short cervix

( Young Li Kim ),( Young Jae Lee ),( Hee Young Cho ),( Eun Ah Kim ),( Min Jung Baek ),( Young Ran Kim ),( Sukho Kang ),( Ji Yeon Lee ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: To determine the differences in pregnancy outcomes among cases treated by vaginal progesterone, intramuscular(IM) progesterone and conservative management in twin pregnancies with a short cervix. 방법: This is a retrospective study of 273 twin pregnancies complicated by a short cervix(<2.5cm) who delivered from 2007 to 2016 in CHA Bundang Medical Center. Women who received cervical cerclage were excluded. Treatment groups included 1) group I; conservative management group without progesterone treatment(n=174), 2) group II; vaginal progesterone suppository group(n=30), 3) group III; IM progesterone injection group(n=69). Primary outcomes were spontaneous birth at <28, 32, 34, or 36 weeks of gestational age(GA). The secondary outcomes included hospitalized for tocolytics or antenatal corticosteroids, small for gestational age and low APGAR score(<7) at 5 min. 결과: Preterm birth before 36 weeks of GA was different among 3 groups(29.3%[51/174] vs.20.0%[6/30] vs.43.5%[30/69], p=0.034). Low APGAR score(<7) at 5min was significantly different among 3 groups(7.8%[27/348] vs.5.0%[3/60] vs.15.2%[21/138], p=0.018). After multivariate analysis, preterm birth(<36 weeks) occurred more frequently in group III than group I(aOR 6.90 95%CI:1.06-45.04, p=0.044). However, there was no significant difference between group II and III. Meanwhile, there were more cases with low APGAR score(<7) at 5 min in group III than group I(aOR 6.58 95%CI:1.43-30.21, p=0.015) and group II(aOR 17.04 95%CI:1.56-185.74, p=0.020) after multivariate analysis. 결론: In twin pregnancies complicated by short cervical length, IM progesterone group showed significantly greater occurrence of preterm birth before 36 weeks of GA in comparison with vaginal progesterone group and conservative management group.

In vivo study for the hemostatic efficacy and foreign body reaction of a new powder-type polysaccharide hemostatic agent

Yoonhyeong Byun,Eun Jin Kim,Areum Lee,Young-Ah Suh,Hee Ju Sohn,Jung Min Lee,Jae Seung Kang,Yoo Jin Choi,Youngmin Han,Hongbeom Kim,Wooil Kwon,Jin-Young Jang 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.102 No.2

Purpose: Various hemostatic agents have been introduced in therapy as postoperative bleeding is a poor prognostic factor for postoperative outcomes. These products can be divided into those that directly promote the hemostatic cascade and those that physically form a barrier by absorbing blood. The latter, powder-type hemostatic agents have the advantages of being inexpensive and more absorbable with less foreign body reactions (FBRs) and are applicable to a relatively wide area. This study was conducted to verify the safety and efficacy of a newly invented polysaccharide product (OOZFIX, Theracion Biomedical), which improves blood absorption and hemostatic effects. Methods: Two separate animal experiments were performed. The first evaluated FBRs histologically at 3 days, 2 weeks, and 4 weeks, after implantation of OOZFIX in rats, and the second compared hemostatic performance of OOZFIX and Arista AH (Bard) in the porcine liver punch biopsy model. Results: We found minimal FBRs in the 3-day group and no reactions in both the 2-week and 4-week groups after implantation of hemostatic agents. The time to hemostasis of OOZFIX was not significantly different from that of Arista AH (median [interquartile range]: 9 [6–10] minutes vs. 8 [6–10] minutes, respectively; P = 0.522). When comparing the serial bleeding grade tendency, there was no statistical difference between OOZFIX and Arista AH (P = 0.656). Conclusion: OOZFIX caused a minimal FBR that disappeared within 2 weeks in vivo, and its hemostatic performance was comparable with that of an existing agent, Arista AH. Further clinical studies are required in the future.

夜間部學生들의 疲勞症狀과 體育授業의 效用性에 關한 考察 : 女子高校生을 中心으로 Centered on the Girls' High School Students

李敏炯,金雅子 慶北大學校 師範大學 1976 敎育硏究誌 Vol.18 No.-

In order to find out the fatigue situations of evening students and the physical training class, we have surveyed and experimented on the 198 exemplary students of evening class attending Sang Seo Girls' High School. And the result of it is as follows: 1. 95% of students have the fatigue symptoms, and most of them have got the mental and nervous sensitive fatigue rather than physical one. 2. The cause of fatigue comes from the lacking of sleep. 3. For the sake of quick recovery from fatigue, the classes of physical education, finf art, and music are far better to be taught at the beginning class. 4. It is recommended that the joyful and interesting contents of text book are to be taught at physical education class. 5. We feel that the physical training classes are needed more to be put in the schedule. In conclusion, the efficiency of physical training class for evening girls high school students seems to be great. This result can be felt true by students themselves. Accordingly, it is highly recommended that the more minute plan and schedule as well as management on physical education are necessary to be considered for the sake of their situations and demands.