심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

위절제술 환자의 표준진료지침 개발 및 적용 효과

김은희,김철규,이순교,김순덕,이혜옥,권정순,이경미,이민미,심순미,유용만,신종식,강은희,이상일,김병식,오성태,육정환,박수길 한국의료QA학회 2003 한국의료질향상학회지 Vol.10 No.2

Background : Gastric cancer is the most common malignant tumor in Korea. surgical operation is one of the major treatment modalities for gastric cancer patients. Therefore, gastrectomy is one of the most common procedures in General Surgery. There were variation in length of hospital stay and medical treatment for gastrectomy between three surgeons at Asan Medical Center. Clinical pathways have received considerable attention as a tool for recucing the medical practice variation, increasing the efficiency of care process, and improving the quality of care. The aim of this study was to evaluate the effect of a clinical pathway for gastrectomy in gastric cancer patients. Methods : The clinical pathway for gastrectomy was developed and implemented by a multidisciplinary group in Asan Medical Center. A computerized clinical pathway program was developed and revised after a pilot test. A total of 145 patients underwent gastrectomy by three surgeons at Asan Medical Center. We compared the length of hospital stay, patient satisfaction, and unplanned readmission rate between the pre-pathway group(n=67) and the post-pathway group(n=78). We also investigated the degree of satisfaction among the physicians and nurses who were main end-users of the clinical pathway. Results : The clinical pathway was applied to all target patients. The average length of hospital stay was shortened from 12.7days to 10.6days(p<0.01). The degree of patient satisfaction with the care process changed from 90.3% to 89.2% after the implementation of the clinical pathway, but the difference was of satistically significant(p=0.761). Unplanned readmission rate was 2.9% in the pre-pathway group. More than 90% of physicians and nurses answered that the clinical pathway had been a useful tool in their medical practice. Conclusions : The findings of the study demonstrated that implementation of the clinical pathway for gastrectomy produced substantial reduction in the length of hospital stay while improving the quality of patient outcomes. The computerized clinical pathway program can be used as one of the powerful patient management tools for reducing the practice variations and increasing the efficiency of care process in Korea hospital settings.

탱고 backward walk 동작시 신발 굽 유형에 따른 하지의 근전도 분석

정미라,서국웅,강영택,염창홍 한국운동역학회 2002 한국운동역학회지 Vol.12 No.1

The purpose of this research is to provide scientific training guides and the basis for preventing injuries in performing tango backward walk by comparing the effects the height and shape of a heel will have on leg muscles and determining the difference between skilled and unskilled dancers through EMG analysis according to different types of shoes Total eight female athletes were selected for this research. Four with six years of professional dancing experience and another four with one year of dance sports experience. Utilizing the EMG system, rectus femoris, biceps femoris, anterior tibialis, gastrocnemius were measured under different heights (0cm, 5cm, 7cm, 9cm) and shapes (flat, fine, wide) of a heel. Experiments were conducted calculating EMG peak value and muscle load ration under different types of shoes in order to analyze the EMG of leg muscles and Erector Spinae when performing tango backward walk. The result was as follows: 1. The EMG peak value of leg muscles showed significant difference under different heights of a heel in anterior tibialis(p<.001) and gastrocnemius(p.<01) in case of skilled dancers, and in biceps femoris(p.<01) in case of unskilled dancers. 2. The EMG peak value of leg muscles showed significant difference under different shapes of a heel in anterior tibialis(p<.001) and gastrocnemius(p<.001) in case of skilled dancers, and showed no significant difference in case of unskilled dancers. 3. The muscle load ration of leg muscles showed different degrees of muscle contribution between skilled and unskilled dancers. The muscle load ration was in the order of anterior tibialis, rectus femoris, biceps femoris, and gastrocnemius in case of skilled dancers and anterior tibialis, gastrocnemius, biceps femoris, and rectus femoris in case of unskilled dancers.

개조개, 백합, 피조개 및 꼬막 발의 조직학적 구조 및 점액질 성상에 관한 조직화학적 연구

강미경,정길남,조운복 釜山大學校 師範大學 科學敎育硏究所 2005 科學敎育硏究報 Vol.32 No.-

개조개, 백합, 피조개 및 꼬막 발의 점액질 성상을 PAS 반응, AB pH 2.5 및 AB pH 2.5-PAS, AB pH 1.0, AF pH 1.7-AB pH 2.5 염색 등의 prelectin법으로 관찰하였다. 개조개, 백합, 피조개 및 꼬막 발은 상피, 상피하결합조직 및 근층의 3층으로 구성되었으며 모든 종에서 발의 표면은 단층섬모원주상피로 덮여 있었다. 백합의 발샘의 점액샘꽈리는 작은 구형이고 소수이며 산재성이었으나 그외 종에서는 큰 구형이고 다수이며 밀집해서 나타났다. 발샘의 도관은 개조개와 피조개가 백합과 꼬막보다 더욱 발달되어 있었으며 피조개와 꼬막은 발의 선단부에 깊이 파인 홈이 나타났으며 흠에 접한 상피하결합조직에 작은 구형의 과립이 밀집해 있었다. 상피세포의 섬모에서 개조개와 백합은 강sulfomucin을, 각각 소량, 소량 내지 중등량 함유하고 있었으며 피조개와 꼬막은 sialomucin과 중성점액질을 중등량 내지 소량 함유하고 있었다. 상피 세포의 세포질은 백합에서만 강 sulfomucin을 미량 내지 소량 함유하고 있었다. 발샘의 도관은 개조개와 백합은 강sulfomucin만을 소량 내지 중등량, 피조개와 꼬막은 소량의 중성점액질과 강 sulfomucin을 중등량 또는 중등량 내지 상당량을 함유하고 있었다. 발샘의 점액샘꽈리에서 개조개 및 백합은 강sulfomucin과 약sulfomucin이 섞여 있었으며 개조개는 중등량 내지 상당량, 백합은 소량 내지 중등량 이었고 피조개와 꼬막은 강sulfomucin만을 함유하였는데 피조개는 중등량 내지 상당량을, 꼬막은 상당량 내지 다량이었다. 발의 결합조직 과립은 피조개에서 상당량 내지 다량의 강sulfomucin을, 꼬막에서 상당량 내지 다량의 중성점액질과 강sulfomucin을 함유하였다. The histochemical properties of mucins of the foot of Saxidomus purpuratus, Meretrix lusoria, Scapharca broughtonii and Tegilltarca granosa were studied prelectin methods, i.e., PAS, AB pH 2.5, Ab pH 2.5-PAS, AB pH 1.0 and AF pH l.7-ABe pH 2.5 stainings. The foots of Saxidomus purpuratus, Meretrix lusoria, Scapharca broughtonii and Tegillarca granosa were composed of three layers, i.e., epithelium, subepithelial connective tissue and muscular layers. The epithelia were lined by simple ciliated columnar cells. The foot gland of Meretrix lusoria consist of multicellular mucous acini were small spheroid in shape and a few in number and scattered, while these acini of other species were large spheroid in shape and were numerous and crowed within connective tissue. The ducts of foot glands were more developed in Saxidomus purpuratus and Scapharca broughtonii than in other species, Particularly, Scapharca broughtonii and Tegillarca granosa showed a deep groove in apical portion of the foot and small spherical granules were found in the subepithielial connective tissue in this portion. The cilia of epithelial cells of the foot gland in Saxidomus purpuratus and Meretrix lusoria were contained only small amounts or small to moderate amounts strong sulfomucins, respectively, while these alia in Scapharca broughtonii and Tegillarca granosa were contained moderate amounts or small to moderate amounts of sialomucins and neutral mucins, respectively. The epihtelial cytoplasm of Meretrix lusoria contained only minimal to small amounts of strong sulfomucins. The ducts of the foot glands in Saxidomus purpuratus and Meretrix lusoria were contained only small to moderate amounts of strong sulfomocins, while these ducts of other spicies were contained small amounts of neutral mucins and moderate or moderate to considerable amounts of strong sulfomucins. The mucous acini of these glands in Saxidomus purpuratus and Meretrix lusoria were contained moderate to considerable and small to moderate amounts of strong and weak sulfomucins, respectively. however, these acini were contained moderate to considerable amounts of strong sulfomucins in Scapharca broughtonii and considerable to large amounts of strong sulfomucins in Tegillarca granosa. The granules within connective tissue of the foot in Scapharca broughtonii were contained considerable to large amounts of strong sulfomucins, but these anules in Tegillarca ganosa were contained considerable to large amounts of neutral mucins and strong sulfomucins.

불량 단감을 이용한 속성 감식초의 제조와 품질 평가

정용진,신승렬,강미정,서지형,원충연,김광수 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.2

This study was performed to analyze general components such as, organic acids, alcohols and minerals in persimmon vinegar made with quick fermented using deteriorated sweet persimmon and four commercial vinegars(rice vinegar, apple vinegar, brewed vinegar, traditional persimmon vinegar) purchased in local markets for quality evaluation. The pH and total acidity of all vinegars were in the range of 2.02~3.02 and 4.62~9.78%, respectively. The pH of quick fermented persimmon vinegar(A) was relatively higher than that of others. Acidity was the highest in brewed vinegar. Total sugar content was in the range of 0.45~6.43%. These contents were high in were high in traditional persimmon vinegar wherase low in brewed vinegar. Total nitrogen and amino-nitrogen were in the range of 0.025~0.046% and 0.015~0.029%. Organic acids were identified as acetic acid, citric acid, tartaric acid, malic acid, succinic acid, formic acid and oxalic acid. While major minerals of all vinegars were Mg, Ca, and Na, minor minerals were Zn, Cu, Mn and Fe.

감과실 알코올 발효를 위한 효모의 분리

정용진,서권일,신승렬,서지형,강미정,김광수 동아시아식생활학회 1997 동아시아식생활학회지 Vol.7 No.4

To utilize abundant poor persimmon fruits effectively, it was studied on alcohol fermentation strains which were isolated and indentified from poor persimmon fruits. The strains which had exellent alcohol production ability were analysized morphogical, cultural characteristics and identified Saccharomyces cerevisiae YJK 20, Sacahroimyces kluveri DJ 97. Alcohol production ability between the isolated strains and industrial alcohol yeasts was compared in the YPD medium, persimmon extract medium. Isolated strains had high alcohol production ability in both of two medium, respectively. So it was expected that the strains, YKJ 20 and DJ 97 had good alcohol fermentation ability of persimmon fruits.

女性의 基本欲求와 衣裳價値觀間의 相關關係 硏究

姜京子,李貞淑,秋美先 慶尙大學校 1987 論文集 Vol.26 No.2

This was designed in order to find out the correlation between the basic needs and values on teh clothes of women, 358 women living in Taegu city and JinJu city were selected as sample for this study. The major findigs of this research were as followings. 1. Women have stronger needs for physiology, self-actualization belongness and affection self-esteem and safety. They regards economic, sensory values most important. 2. Young women tend to regard aesthetic values such as form,color, harmony important. Older women like to wear comfortable clothes. 3. Women having higher education have lower needs for physiology and safety, and they have strong need for self-actualization, And they tend to regard aesthetic values important, but they have lower religious values on the clothes. 4. There is difference of need and clothing values of women depending on the monthly income of the family. 5. Unemployed women have stronger need for self-actualization than the employed women. Ⅱ.Correlation between basic needs and values on clothes was investigated. 1. Married women who regard aethetic values important have stronger need for self-actualization. Single women who regard economic values important have lower needs for safety and self-actualization but married women have lower needs for belongingness and self-esteem. 2. There is a little difference of correltion between values and needs depending on the age and education of respondents. 3. There is a difference of correlation between values and needs depending on the monthly incomes of respondents.