Original Article: Biochemistry/Molecular Biology : Various Pathogenic Pseudomonas Strains that Cause Brown Blotch Disease in Cultivated Mushrooms

( Lin Lin Mu ),( Yeong Bae Yun ),( Soo Jin Park ),( Jae Soon Cha ),( Young Kee Kim ) 한국응용생명화학회 2015 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.4

Brown blotch disease in cultivated mushrooms is caused by Pseudomonas tolaasii, which secretes a lipodepsipeptide, tolaasin. Tolaasin is a pore-forming toxin in the cell membranes, thus destroying the fruiting body structure of mushroom. In this study, we isolated pathogenic bacteria from mushrooms that had symptoms of brown blotch disease. In order to identify these bacteria, their 16S rRNA genes were sequenced and analyzed. Pathogenic bacteria identified as Pseudomonas species were thirty five and classified into five subgroups: P1 to P5. Each subgroup showed different metabolic profile measured by API 20NE kit. Fifty percent of the bacteria were identified as P. tolaasii (P1 subgroup). All five subgroups caused the formation of brown blotches on mushroom tissues and the optimum temperature was 25oC, indicating that they may be able to secrete causal factors, such as tolaasin and similar peptide toxins. These results show that there are at least five different pathogenic Pseudomonas species as blotch-causing bacteria and, therefore, strains from the P2 to P5 subgroups should be also considered and studied as pathogens in order to improve the quality and yield of mushroom production.

To Prevent Crack for Thin-wall Workpieces during Milling Process by Torque Feedback

Mu-Ping Tsai,Nan-Chyuan Tsai,Chun-Chi Lin,Hsin-Lin Chiu 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10

Thin-wall tubular-geometry workpieces have been widely applied in aircraft and medical industries. Though numerous research reports are available that the tool path can be planned on the basis of preset surface profile before actual milling operation is performed, it is still difficult to predict the real-time surface profile errors for peripheral milling of thin-wall tubular workpieces. This research is focused on how to real-time formulate the appropriate applied cutting torque via feedback of spindle motor current. A few suitable cutting conditions which are able to prevent potential break/crack of thin-wall workpieces and enhance productivity but almost retain the same cutting quality is proposed. To achieve this goal, estimated surface profile error on machined parts due to deflections caused by both tool and workpiece is studied. The torque estimation approach by spindle motor current feedback and the corresponding fuzzy logic controller are employed. Compared with constant cutting torque during milling operation in tradition manner, it is observed that the time consumption of milling cycle by aid of the aforesaid fuzzy logic controller is greatly shortened while the resulted cutting accuracy upon finish of workpiece can be almost retained.

CNOP-Based Sensitive Areas Identification for Tropical Cyclone Adaptive Observations with PCAGA Method

Lin-Lin Zhang,Shi-Jin Yuan,Bin Mu,Fei-Fan Zhou 한국기상학회 2017 Asia-Pacific Journal of Atmospheric Sciences Vol.53 No.1

In this paper, conditional nonlinear optimal perturbation (CNOP) was investigated to identify sensitive areas for tropical cyclone adaptive observations with principal component analysis based genetic algorithm (PCAGA) method and two tropical cyclones, Fitow (2013) and Matmo (2014), were studied with a 120 km resolution using the fifth-generation Mesoscale Model (MM5). To verify the effectiveness of PCAGA method, CNOPs were also calculated by an adjoint-based method as a benchmark for comparison on patterns, energies, and vertical distributions of temperatures. Comparing with the benchmark, the CNOPs obtained from PCAGA had similar patterns for Fitow and a little different for Matmo; the vertically integrated energies were located closer to the verification areas and the initial tropical cyclones. Experimental results also presented that the CNOPs of PCAGA had a more positive impact on the forecast improvement, which gained from the reductions of the CNOPs in the whole domain containing sensitive areas. Furthermore, the PCAGA program was executed 40 times for each case and all the averages of benefits were larger than the benchmark. This also proved the validity and stability of the PCAGA method. All results showed that the PCAGA method could approximately solve CNOP of complicated models without computing adjoint models, and obtain more benefits of reducing the CNOPs in the whole domain.

Overview of insect biodiversity in the Northeast China:Lepidoptera

Mu-jie Qi,Hui-lin Han,Yang-Seop Bae 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

The Northeast of China, which covers an area of 124'1400 km2, comprises HeiLongjiang, JiLin, LiaoNing province, and northeast of inner Mongolian. The annual mean temperature is 5 centigrade below zero in the north, and 10 centigrade above zero in the south. The annual precipitation range from 300 mm to 1200 mm. Moreover, northeast China is an important forest area in China, and hundreds of forest disease and pests occurred every year. Among these pests, the Lepidoptera account for a big part, accordingly, the research on diversity of Lepidoptera is the basis of taxonomy and pests control. According to 「List of Chinese Insects」(Hua, 2005), there are about 20,692 species in China, and 2,934 in Northeast China, for instance, Noctuidae 1,400 spp. (Hua, 2005), Notodontidae 128 spp. (Hua, 2005), Tortricidae 267 spp. (Hua, 2005), Pyralidae 146 spp. (Hua, 2005), Geometridae 233 spp. (Zheng, 1999), Hesperiidae 54 spp. (Zhou, 2000), Parnassiidae 32 spp. (Zhou, 2000), Papilionidae 26 spp. (Zhou, 2000), Pieridae 59 spp. (Wang, 1998), Nymphalidae 188 spp. (Zhou, 2000), Lycaenidae 150 spp., etc. (Wang, 2002).

Two-dimensional dynamic analysis of sandwich plates with gradient foam cores

Lin Mu,Dengbao Xiao,조종두,Guiping Zhao 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.9

Present investigation is concerned about dynamic response of composite sandwich plates with the functionally gradient foam cores under time-dependent impulse. The analysis is based on a model of the gradient sandwich plate, in which the face sheets and the core adopt the Kirchhoff theory and a {2, 1}-order theory, respectively. The material properties of the gradient foam core vary continuously along the thickness direction. The gradient plate model is validated with the finite element code ABAQUS®. And the results show that the proposed model can predict well the free vibration of composite sandwich plates with gradient foam cores. The influences of gradient foam cores on the natural frequency, deflection and energy absorbing of the sandwich plates are also investigated.

The Environmental Policy Stringency in Taiwan and Its Challenges on Green Economy Transition

Mu-Xing Lin,Tsung-Yi Lee,Kuei-Tien Chou 서울대학교 사회발전연구소 2018 Journal of Asian Sociology Vol.47 No.3

With the aim of promoting green economic transition on a national level, this study applies the environmental policy stringency index, developed by OECD, to analyse Taiwan’s energy policies between 1990 and 2015, and compare results with those of other OECD countries. After examining the institutional foundation and regulatory tools in Taiwan’s green economy transition, we found that green transition in Taiwan currently faces eight major challenges in common with other OECD member states. We argue that the government of Taiwan needs to make adjustments to its environmental policy or follow OECD trends and strengthen environmental regulations. However, we also argue that the goal of green economic transition will never be easy to reach as long as a nation remains heavily dependent on “non-market mechanisms.” The establishment of the OECD environmental policy stringency provides an international standard by which individual countries can compare themselves to measure their development.

rs10505474 and rs7837328 at 8q24 Cumulatively Confer Risk of Prostate Cancer in Northern Han Chinese

Zhang, Lin-Lin,Sun, Liang,Zhu, Xiao-Quan,Xu, Yong,Yang, Kuo,Yang, Fan,Yang, Yi-Ge,Chen, Guo-Qiang,Fu, Ji-Cheng,Zheng, Chen-Guang,Li, Ying,Mu, Xiao-Qiu,Shi, Xiao-Hong,Zhao, Fan,Wang, Fei,Yang, Ze,Wang, Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Aims: Genome-wide association studies (GWAS) have identified several risk variants for prostate cancer (pCa) mainly in Europeans, which need to be further verified in other racial groups. We selected six previously identified variants as candidates and to define the association with PCa in Northern Han Chinese. Methods: 749 subjects from Beijing and Tianjin in Northern China were included. Six variants (rs10505474, rs7837328, rs4242384, rs7813, rs486907 and rs1058205) were genotyped by high resolution melting (HRM) assays. The individual and cumulative contribution for of the risk of PCa and clinical covariates were analyzed. Results: Among the six candidate variants, onlyrs10505474, and rs7837328, both locating at 8q24 region, were associated with PCa in our population.rs10505474 (A) was associated with PCa ($OR_{recessive}=1.56$, p=0.006); and rs7837328 (A) was associated with PCa ($OR_{dominant}=1.38$, p=0.042/$OR_{recessive}=1.99$, p=0.003). Moreover, we observed a cumulative effects between them ($p_{trend}=2.58{\times}10^{-5}$). The joint population attributable risk showed the two variants might account for 71.85% of PCa risk. In addition, we found the homozygotes of rs10505474 (A) and rs7837328 (A) were associated with PCa clinical covariants (age at onset, tumor stage, respectively) ($p_{age}=0.046$, $P_{tumorstage}=0.048$). Conclusion: rs10505474 (A) and rs7387328 (A) at 8q24 are associated with PCa and cumulatively confer risk, suggesting the two variations could determine susceptibility to PCa in the Northern Chinese Han population.

Simulation analysis and evaluation of decontamination effect of different abrasive jet process parameters on radioactively contaminated metal

Zhong Lin,Deng Jian,Zuo Zhe-wen,Huang Can-yu,Chen Bo,Lei Lin,Lei Ze-yong,Lei Jie-heng,Zhao Mu,Hua Yun-fei 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.11

A new method of numerical simulating prediction and decontamination effect evaluation for abrasive jet decontamination to radioactively contaminated metal is proposed. Based on the Computational Fluid Dynamics and Discrete Element Model (CFD-DEM) coupled simulation model, the motion patterns and distribution of abrasives can be predicted, and the decontamination effect can be evaluated by image processing and recognition technology. The impact of three key parameters (impact distance, inlet pressure, abrasive mass flow rate) on the decontamination effect is revealed. Moreover, here are experiments of reliability verification to decontamination effect and numerical simulation methods that has been conducted. The results show that: 60Co and other homogeneous solid solution radioactive pollutants can be removed by abrasive jet, and the average removal rate of Co exceeds 80%. It is reliable for the proposed numerical simulation and evaluation method because of the well goodness of fit between predicted value and actual values: The predicted values and actual values of the abrasive distribution diameter are Ф57 and Ф55; the total coverage rate is 26.42% and 23.50%; the average impact velocity is 81.73 m/s and 78.00 m/s. Further analysis shows that the impact distance has a significant impact on the distribution of abrasive particles on the target surface, the coverage rate of the core area increases at first, and then decreases with the increase of the impact distance of the nozzle, which reach a maximum of 14.44% at 300 mm. It is recommended to set the impact distance around 300 mm, because at this time the core area coverage of the abrasive is the largest and the impact velocity is stable at the highest speed of 81.94 m/s. The impact of the nozzle inlet pressure on the decontamination effect mainly affects the impact kinetic energy of the abrasive and has little impact on the distribution. The greater the inlet pressure, the greater the impact kinetic energy, and the stronger the decontamination ability of the abrasive. But in return, the energy consumption is higher, too. For the decontamination of radioactively contaminated metals, it is recommended to set the inlet pressure of the nozzle at around 0.6 MPa. Because most of the Co elements can be removed under this pressure. Increasing the mass and flow of abrasives appropriately can enhance the decontamination effectiveness. The total mass of abrasives per unit decontamination area is suggested to be 50 g because the core area coverage rate of the abrasive is relatively large under this condition; and the nozzle wear extent is acceptable

노인요양시설의 공간기능별 색채환경 연구

정무린(Jeong, Mu Lin),박혜경(Park, Hey Kyung) 한국색채학회 2017 한국색채학회 논문집 Vol.31 No.2

Nursing homes are elderly medical welfare centers that provide personal care for the elderly who have difficulties with their daily lives due to the decline of physical and mental function. An environment plan functions that can functionally support the elderly is needed. Proper use of color can have an ancillary role in helping the elderly"s cognition of objects and thoughts, because proper color can maintain the elderly"s physical health and psychological stability. The purpose of this research is to understand the characteristics of various color environment in nursing homes, with the intention of producing a practical color guideline for future nursing home construction. It is expected to be used as a basic guideline data in the nursing home"s color plan. 8 nursing homes with a minimum total floor area of 2,000㎡ and patients 30 and above were selected for the examination. The color measurement space range was divided based on the space functions which are living area, nursing area, and common use area. The color environment characteristic of the nursing homes was analyzed, and result is stated in the following: 1) since most of the examined 8 nursing homes were concentrated in the use of YR, Y color shades, there was a need for subdividing the colors and introducing diverse color, 2) colours with high value and chroma should be used considering the deterioration of elder"s eyesight.

Taurine Attenuates Hepatic Inflammation in Chronic Alcohol-Fed Rats Through Inhibition of TLR4/MyD88 Signaling

Chao-Jen Lin,Chun-Ching Chiu,Yi-Chen Chen,Mu-Lin Chen,Tsai-Ching Hsu,Bor-Show Tzang 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.12

Accumulating evidence indicates that overconsumption of ethanol contributes in many ways to the pathogenesis of hepatic injury. Although studies indicate that taurine decreases lipogenesis, oxidative stress, and inflammatory cytokines, the protective effect of taurine against alcohol-induced liver injury is still unclear. To clarify the precise signaling involved in the beneficial effect of taurine on alcohol-induced liver injury, rats were randomly divided into four treatment groups: (1) control (Ctl), (2) alcohol (Alc), (3) Alc + taurine (Tau), and (4) Alc + silymarin (Sil). The Tau and Sil groups had lower lymphocyte infiltration and significantly lower TLR-4/MyD88 and IκB/NFκB compared to the Alc group. The inducible nitric oxide synthase (iNOS), C-reactive protein (CRP), tumor necrosis factors (TNF)-α, interleukin (IL)-6, and IL-1β were also significantly lower in the Tau and Sil groups than in the Alc group. The experimental results indicated that hepatoprotection against alcohol-induced inflammation may be mediated by decreased TLR-4/MyD88 signaling.