해수에서 분리한 Vibrio sp. M-96 균주의 열감수성 alkaline phosphatase 성질

박문경,진덕희,김중균,공인수,김광현,홍용기 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1

A thermolabile alkaline phosphatase has been purified through steps of osmotic shock, ammonium sulfate salting-out, and DEAE-cellulose chromatography from the cultured broth of the marine Vibrio sp. M-96 strain. The optimal temperature for the enzyme activity was 35℃. The optimal pH was pH 11.0, and the range of pH stability was pH 10.4 to 12.0. Thermal inactivation occured within 6 minutes at 60℃. The enzyme was considerably inactivated by 0.1mM concentrations of Hg^2+, Ni^2+ and Zn^2+, whereas activated up to 234% by 1mM of Mn^2+. The activation energy and deactivation energy by the Arrhenius equation were 4.02 Kcal/mol and 9.09 Kcal/mol, respectively. The Km and Vmax values of the enzyme for p-nitrophenylphosphate were found to be 0.0465mM and 0.001335mM/min. respectively. Active from of the enzyme had a molecular weight of 57,000 dalton determined by the Sephadex G-200 gel filtration method.

장기투석을 받은 만성 신부전 환자에서 발생한 종양에 관한 고찰

황정화,이혜경,홍현숙,박재성,김대호,권귀향,최득린,황승덕,이희발 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.2

The authors tried to evaluate tumor occurrence in long-term dialysis patients with chronic renal failure. Among 359 patients, 20 patients (about 5.6%) were diagnosed with malignancy during long-term dialysis from the period of 1983 to 1995 at our nephrology department. The ultrasonographic and computed tomographic findings including the clinical features of 20 patients that were retrospectively reviewed. The mean age of the patients was 53 (37-75)years old and the ratio of male to female was 9:1. Among the 20 cases, 7 cases of hepatoma (35%) were developed. Among them, urinary tract tumors such as renal and bladder cancer were developed in 4 (20%) and 2 (10%) of the cases. Other malignant tumors were lymphoma, stomach cancer, uterine cervical cancer, cholangiocarcinoma, lung cancer, meningioma, and acoustic neuroma one case of each (each of 5.3%). The most common tumor in patients with chronic renal failure, who were receiving long-term dialysis, was hepatoma and the second most common tumor was cancer of the urinary tract such as kidney and bladder.

유방암과 섬유선종의 방사선학적 소견 : 유방 단순촬영술과 유방 초음파검사 소견의 비교 연구 Studies on mammographic and ultrasonographic findings evaluation

임한혁,최득린,고은석,이혜경,임철완,이상진,홍현숙,최교창,김영화,박재성 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Purpose : To evaluate the utility of mammography and ultrasonography in study of morphologic differential diagnosis between malignant and benign mass. Materials and Methods : Average age of patients with breast cancer was 48.4 year-old, and that of fibroadenoma was 29.4 year-old. The equipments used for examination were MF-159 high frequency mammography(Bennet Co.) and Acoustic image 5200S. We evaluated mammographic findings about breast parenchymal pattern, calcification, margin and size of the mass associated tumor nature, and ultrasonographic findings about shape, height and width, internal homogenicity and echo pattern, wall contour, boundary echo, and posterior shadow in breast mass. Thirty-seven of mammographic findings and 34 cases of ultrasonogreaphic findings in pathologically proven breast cancer and 34 case of mammographic findings and 71 cases of ultrasonographic findings in breast fibroadenoma were reviewed retrospectively. Results : In mammographic study, the parenchymal patterns of breast cancer according to Wolfe classification were NI(16.2%), PI(24.3%), P@(37.8%), and DY(21.6%) and those in fibroadenoma were NI(3.1%), Pl(11.7%), P2(35.2%),and DY(50%). Mammographic findings about presence of calcification were as follows; only calcification(16.2%), only mass(51.3%), and mass with calcification(32.5%) in breast cancer, and only mass(91.2%), mass with calcification(8.8%) in fibroadenoma. The features of mass margins were classified as ill-defined margin(45.9%), well-defined(5.4%), and well-defined with partial spiculation(48.6%) in breast cancer and ill-defined(20.5%), well-defined(73.5%), and well-defined with partial spiculation(5.8%) in fibroadenoma. The mass were grouped by size; 1-2cm(37.8%), 2-4cm(45.9%), and 4-6cm(16.3%) in breast cancer and 1-2cm(79.4%), 2-4cm(17.6%), and 4-6cm (3.0%) in fibroadenoma. On ultrasonographic study, the shape of masses in breast cancer were lobulated(82.3%),oval(14.7%), and round(3.0%),and those in fibroadenoma were lobulated(14.0%), oval(57.7%), and round(18.3%). The cases that the height was longer than width were 32.3% in breast cancer and 0% in fibroadenoma. The findings about homogenecity and echo patterns were as follows; hypoechoic(52.9%) and mixed(47.1%) in breast cancer and hypoechoic(2.8%), hypoechoic(63.4%), and mixed(33.8%) in fibroadenoma. The margin of masses were smooth in 85.9% of fibroadenoma, and irregular in 73.6% of breast cancer. The boundary echoes of masses and decreased posterior echo shadow were seen only in 61.8% and 55.9% of breast cancer, respectively. Conclusion: The margin and calcifications are more clearly delineated on mammography but breast cancer and fibroadenoma, both are more commonly developed in dense parenchymal pattern(P2+DY),and there findings are easily obscured in mammography. So ultrasoography is very useful in these masses evaluation, especially amoung young patients.

신동맥 협착 정도에 따른 도플러 초음파 소견

박재성,이혜경,김대호,최득린,권귀향,홍현숙,임한혁,김기정 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

Renal artery stenosis is the one of causes of hypertension in a small percentage of all patients but is only anatomical cause that can be treated by means of surgery and percutanecous transluminal angioplasty. Angiography is the golden standard for diagnosis of renal artery stenosis, but a little invasive. With the advent of abdominal Doppler ultrasonography, renal artery stenosis could be diagnoed noninvasively. The experienced hands appears to be valuable in the doppler examination, ut some investigators consider it almost useless. The authors studied the effect of stenosis of renal artery on the downstream intrarenal blood flow, a snare was placed around renal artery in 12 rabbits and compressed to create stenosis while Doppler ultraonographic curve and flow index were obtained from main renal and interlobar arteries by means of placement of 10-MHz curved linear tranducer directly on the surface of kidney. The Doppler measurements were compared with the percentage of stenosis on angiograms. The results were as follow: 1. Ten satisfactory angiograms and Doppler ultrasonograms were obtained. 2. Three renal arteries were created stenosis below 70% (Group A), Seven were above 70% (Group B). 3. The ranges of RI (resistance index) were 0.61-0.86 (mean 0.78±0.154) at main renal arteries, 0.57-0.85 (mean 0.77±0.231) at interlobar artery. The range of PS (peak systolic velocity) were 13.1-28.8 (mean 21.65±4.445) m/sex at main renal artery, 5.2-18.1 (mean 11.74±4.095) m/sec at interlobar arteries. The RI of rabbits were higher than those of human (mean 0.58±0.05). 4. The range of RI in stenotic arteries of group B were 0.45-0.74 (mean 0.57±0.143) at main renal artery, 0.52-0.65 (mean 59±0.051) at interlobar arteries. The range of PS were 11.2-21.3 (mean 15.04±3.755) m/sec at main renal arte교, 5.7-11.3 (mean 8.24±2.056) at interlobar artery. The difference were statistical y significant (P=0.0302), but without remarkable change in group A. 5. Doppler ultrasonographic curve during progressive compression of renal artery showed dampened systolic waveform and diagonal upstroke at main renal arteries (71.4%) and interlobar arteries (85.7%) in group B, but without remarkable change in group A. 6. Release of the arterial compression caused higher than normal peak systolic velocity initially, at main renal arteries (42.9%) and interlobar arteries (28.6%). but the curve of all rabbits returned to normal within 5 minutes. The Doppler ultrasonographic index and curve were not changed on follow up Doppler ultrasonogram at 5 min. 10 min. 30 min. and 1 hour. The change of the systolic curves that compressed experimentally is identical to change in the pulsus tardus and parvus waveforms seen in both acute and chronic severe renal artery stenosis in humans, can helpul for the diagnosis of renal artery stenosis and the effects of treatment by percutaneous tranluminal angioplasty and surgery.

소아 급성 신우신염환아의 색도플러 초음파 검사의 유용성

김은미,권귀향,이혜경,홍현숙,최득린 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

Purpose: Urinary tract infections are common in childhood and are most frequent in those under 1 year of age. In these children, however, differentiation of the pyelonephritis from cystitis is clinically difficult. We investigated the utility of color doppler image ultrasound in the diagnosis of acute pyelonephritis(APN) in children. Results were compared with renal cortical scintigraphy using 99mTC-DMSA. Materials and Methods : 99mTc-DMSA SPECT and color doppler ultrasound were performed within 1 week intervals in 14 patients with clinically suspected APN. (aged 4months to 15years: mean 6.1 year, 7 girls and 7boys). Urine culture was considered positive if greater than 100.000 colonied of a single organism were isolated. CDI was performed with 3.5NHz convex linear probe Ultramark 9(ATL., Bothell, Washington, USA) following a standard gray scale sonogram of the kidneys. Renal cortical scintigraphy was performed with 99mTc DMSA SPeCT(PRISM 2000, Picker, USA), Axial, coronal, both sagittal pinhole images were obtained 1.5-2 hour after 1mCi isotope was injected. Renal scan was considered to be abnormal and indicative of APN when there was a focal, wedged shaped defect in radionuclide accumulation extending from the periphery of the kidney to centrally or if there was diffuse abnormality in renal tubular function. Renal scarring was defined when defects were shallow lesions along the periphery of the kidney with evidence of volume loss, or if they could be shown from prior examination to pre-date of the acute episode. The color doppler image were interpreted to be consistent with APN if there was decrease flows in the renal cortex. Results: Of the 28 kidneys were examined, renal scan showed 5 cases of abnomal findings, 3 cases were APN, 2 cases were chronic scarring. Two fo the three cases of APN showed decreased blood flow on CDI and positive culture, for a sensitivy 67%. Two cases of renal scarring due to grade V bilateral vesicoureteral reflux show decreased blood flow at right upper pole kidney on CDI, however DMSA scan had diffculties finding the new lesion due to underlying irregularity of scarred kidney. Color doppler US correctly identified 4 of 5 renal poles with APN and 21 of 23with no APN including 2 patients with false positive. The DMSA scan was used as the standard of comparison the study; the CDI has a sensitivity 67%, specificity 84%, positive predictive value 33%, negative predictive value 95%. 84% The lesion showed decreased blood flow and on one follow up case increased blood flow. The presence of prior renal scarring made interpretation of asymmetric vascularity difficult on DMSA, the use of CDI may increase the diabnostic sensitivity of sonography in pediatric pyelonephritis.

3차 의료기관에 입원한 노숙환자에서 감염질환의 유병율

함세운,남기석,노혜진,홍경득,황래석,방지환,신형식 대한감염학회 2007 감염과 화학요법 Vol.39 No.4

목적 : 3차 의료기관에서 입원치료를 받았던 노숙자를 대상으로 감염성 질환의 유병율을 알아보고자 하였다. 재료 및 방법 : 2002년 1월부터 2004년 8월 사이에 국립의료원에서 입원 치료를 받았던 269명의 노숙자 환자들을 대상으로 의무기록을 조사하였다. 결과 : 연구 기간동안 입원치료를 받은 총 269명의 노숙자 환자 중 남자가 249명(92.9%), 여자가 19명(7.1%)이었다. 감염질환의 유병율은 B형 간염이 228명 중 15명(6.6%), C형 간염 113명 중 8명(7.1%), 결핵은 269명중 19명(7.1%), VDRL 양성인 경우는 234명 중 25명(10.7%), 항사람면역결핍바이러스항체 양성은 130명중 2명(1.5%)이었다. B형 간염표면항원에 대한 항체 형성자는 118명(51.7%), 항체 미형성자는 95명(41.7%)이었다. 결핵으로 진단된 19명 중 객담도말 및 배양검사와 조직검사로 확인된 경우가 14명, 균음성이나 임상적으로 의심되는 경우가 5명이었다. 간질환 및 관련된 합병증으로 입원한 환자 28명 중 B형 및 C형 간염바이러스에 관련된 경우는 5명(17.9%)에불과하였고 결핵은 19명중 13명(68.4%)이 폐결핵 및 림프절종대의 증상으로 입원치료를 받았다. 복합 감염의 경우는 총 9명이었는데 후천면역결핍증후군 환자였던 2명중 1명이 결핵에 이환되어 있으면서 VDRL 양성소견을 보였고 다른 1명이 HIV와 결핵의 동시감염이었다. 결론 : 노숙자 입원환자에서 결핵, C형 간염 및 매독의 유병율이 일반 인구에 비해 높았다. 노숙자환자를 담당하는 의료인력를 보호하고 환자의 감염질환 유무를 파악하기 위해 적절한 선별검사가 필요하며 감염질환의 확산을 방지하기 위한 정부의 적극적인 대책이 필요하다. Background : Despite the expected high prevalence of several infectious diseases among the homeless, a majority of them have not received screening test and early treatment. Materials and Methods : The subjects of this study were 269 homeless patients who had been hospitalized in the National Medical Center (Korea) between January 2002 and August 2004. Their medical histories and records were reviewed to analyze the prevalence and clinical characteristics of hepatitis B and C, syphilis, tuberculosis and HIV infection among them. Results : The entire 269 homeless patients being hospitalized during the period of this study consist of 249 male patients and 19 female patients. For the prevalence of infectious diseases, HBV was seen in 15 of 228 patients (6.6%); HCV in 8 of 113 patients (7.1%); VDRL positive in 25 of 234 patients (10.7 %); HIV antibody positive in 2 of 130 (1.5%); and tuberculosis in 19 of 269 (7.1%). The number of patients who had the antibody to hepatitis B was 118 (51.7%). Of 28 patients hospitalized with the chief complaints of liver diseases and/or their complications, the number of cases infected with hepatitis B and C viruses was only 5 (17.9%). On the other hand, for patients with tuberculosis, 13 of 19 cases (68.4%) were hospitalized with the chief complaints associated with symptoms of pulmonary tuberculosis or enlarged lymph nodes. The number of patients with multiple infectious diseases was 9 in total. Conclusion : As expected, the prevalence of some infectious diseases were higher among the homeless inpatients, than non-homeless population. Proper screening tests in order to determine the presence of any infectious diseases among the patients and protect involved medical staff are necessary. Further, the government should take proactive measures to prevent the spread of infectious diseases in the aspect of public health.

KAL기 추락에 의한 대량재해의 개인식별

강신몽,이원태,고영창,최상규,김윤희,이홍석,서재관,윤중진,이혜경,최득린,김종열,윤창육,변명식,이장홍 大韓法醫學會 1991 대한법의학회지 Vol.15 No.2

Individual identification is an important part in medicolegal field especially in mass disaster. At July, 27, 1989, KAL KE-803 was crashed on landing at Tripoli International Airport, Liba. The plane was caught in fire and sixty eight Koreans were sacrified. The majority of victims were severely charred and injured. The authors examed all dead bodies and successfully identified all the cases through visual, anthropological, odontological, radiological and pathological methods including fingerprint and blood typing.

Encapsulating Peritoneal Sclerosis: A Single-Center Experience

( Kyung-deuk Hong ),( Owen Kwon ),( Jang-hee Cho ),( Mi-kyung Jin ),( Ji-young Choi ),( Se Hee Yoon ),( Chan-duck Kim ),( Yong-lim Kim ),( Sun-hee Park ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.-

Encapsulating Peritoneal Sclerosis: A Single-Center Experience

( Kyung Deuk Hong ),( Owen Kwon ),( Jang Hee Cho ),( Mi Kyung Jin ),( Ji Young Choi ),( Se Hee Yoon ),( Chan Duck Kim ),( Yong Lim Kim ),( Sun Hee Park ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1

Background: Encapsulating peritoneal sclerosis (EPS) is a serious and often fatal complication of long-term peritoneal dialysis (PD) with severe malnutrition and poor prognosis. We aimed to investigate the incidence and clinical features of EPS in patients on PD. Methods: The data were collected retrospectively from 603 prevalent PD patients in the Kyungpook National University Hospital between August 2001 and July 2011. EPS was diagnosed by the 2000 criteria of the International Society of Peritoneal Dialysis. Results: Eight patients (1.3%) met the diagnostic criteria of EPS. They had at least one of three symptoms: abdominal pain, vomiting and abdominal distension. Mean age of patients was 48.5 (range: 33 to 65) years and 4 were men. Mean duration on PD was 111.7 (range: 23 to 186) months. Seven patients had chronic glomerulonephritis as underlying renal disease, and one patient hypertensive nephrosclerosis. All patients except one had 2 or more episodes of peritonitis, the peritonitis rate being 1/33.7 patient-months in the 8 EPS patients. Six patients were treated with icodextrin solution. Four patients were treated with beta-blocker (cavedilol, 25-50 mg daily). Total parenteral nutrition was used in all patients. Only one patient had elective surgical intervention, adhesiolysis. Four patients were treated with glucocorticoid (0.5~1 mg/kg/day), three with tamoxifen (10-20 mg daily) and three with both. Of the 8 patients diagnosed with EPS, 6 survived and 2 died, an overall mortality rate being 25%. Conclusion: EPS is rare but a serious life threatening complication in patients on long-term PD. To reduce the incidence and mortality of EPS, careful monitoring and intensive therapy may be needed. Keywords: Encapsulating peritoneal sclerosis, Peritonitis, Continuous ambulatory peritoneal dialysis.

Determination of Amisulbrom Residue in Agricultural Commodities Using HPLC-UVD/MS

Kyung-Geun Ahn,Gyeong-Ha Kim,Gi-Ppeum Kim,Min-Ji Kim,Seula Kim,Young-Sun Hwang,Seung-Beom Hong,Young Deuk Lee,Myoung-Gun Choung 한국농약과학회 2014 한국농약과학회 학술발표대회 논문집 Vol.2014 No.4