Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Stain improvement in the white button mushroom ‘Seolgang’ and its varietal characteristics in Agaricus bisporus

Byung-Joo Lee,Mi-Ae Lee,Yong-Gyun Kim,Kwang-Won Lee,Yong-Pyo Lim,Byung-Eui Lee,Ho-Yeon Song 한국버섯학회 2012 한국버섯학회지 Vol.10 No.4

The button mushroom (Agaricus bisporus) is one of the most widely cultivated important edible mushroom species. In the breeding of new button mushroom, ‘Seolgang’ was developed by crossing two monokaryons ‘CM020913-27’ and ‘SSU423-31’. Because of the secondarily homothallism, only a small percentage of the basidia produce 3 or 4 spores, which are mostly haploid (n) and do not fruit. Single spore cultures derived from these types of spores produce a vegetative mycelium that also contain a variable number of genetically identical nuclei per cell called monokaryon. The lack of clamp connections between monokaryon and dikaryon required a series of mycelial culture and fruiting test. After crossing, hybrids were cultivated on a small scale and on a commercial scale at a farm. For this, the spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of ‘Seolgang’ on CDA was better at 20℃ and 25℃ when it was compared with that of ‘505 Ho’. The mature cap shape of new strain ‘Seolgang’ is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 41.2 mm on average. In comparison with white strain ‘505 Ho’, the strain had a yield that was 9% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 19% firmer with a good shelf life. Days of fruiting body were 3-4 days later than those of ‘505 Ho’. The physical characteristics such as elasticity, chewiness, adhesiveness were better than that of ‘505 Ho’. Genetic analysis of the new strain ‘Seolgang’ showed different profiles compared to ‘505 Ho’, CM02913-27, SSU413-31, when RAPD primers A02 and O04 were used.

Chlorpromazine이 백서 해마에서 전기경련 충격에 의한 MAPK 타이로신 인산화에 미치는 영향

주연호,정성훈,전송희,강웅구,김용식,김상욱,박주배 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.2

Object : In order to examine the interaction mechanisms of electroconvulsive shock(ECS) and antipsychotic drug at the level of molecular biology, we observed the effect of chlorpromazine pre-treatment on the activation of mitogen activated protein kinase(MAPK) induced by electroconvulsive shock(ECS) in rat hippocampus Method : Male Sprague-Dawley rats were divided into 2 groups. To the experimental group chlorpromazine(20㎎/㎏) was given intraperitoneally, and to the control distilled water was given instead. Thirty minutes later, ECS was given and the hippocampus was dissected out 2 minutes thereafter. Immunoblotting with antiphosphotyrosine antibody was carried out, and the signal intensity at 42kDa band was quantitized using densitometer. The obtained result was compared by student t-test between the experimental and the control group. The absolute amount of MAPK was measured by immunoblotting with anti-MAPK antibody. Result : The tyrosine phosphorylation of MAPK reached peak at 2 minutes after ECS. However, in the chlorpromazine pre-treated group, the peak level of MAPK tyrosine phosphorylation was significantly attenuated(t=-3.12, df=14, p=0.008) compared to the control. In contrast to this, the absolute amount of MAPK did not differ between the pretreated and the control group. Conclusion : Chlorpromazine attenuated the tyrosine phosphorylation of MAPK by ECS. This finding seems to be related to the fact that chlorpromazine pre-treatment changed the cfos expression by ECS in rat brain. Antipsychotic drug and ECS might interact at the level of MAPK signal transduction system, and this might explained the observed synergistic effect of two treatment modality.

兩畦編 紡毛織物의 收縮率에 關한 硏究

宋周鎬 충남대학교 공업기술개발연구소 1976 工業技術開發硏究所論文集 Vol.3 No.1

The purpose of the work discribed here was to determine the factors which control the dimensions of relaxed Full-Cardigan woolen fabrics. It is shown experimentally that properties of Full-Cardigan woolen fabrics can be discribed by sets of "K" values similar to those used for plain knitted fabrics.

근위 경골과 골절의 수술적 치료

송경진,양근호,이주홍 대한골절학회 2002 대한골절학회지 Vol.15 No.4

서 론 : 경골과 골절의 치료 목적은 가능한 한 정확한 관절면의 정복, 골절의 견고한 고정과 유지 및 손상된 인대의 안전성을 재건시켜주고 조기 관절운동을 시킴으로써 슬관절의 기능을 회복시키는데 있다. 수술을 시행한 경골과 골절 환자의 치료결과를 비교하고 합병증 등의 임상분석을 통해 향후 치료지침을 제시하고자 하였다. 연구대상 및 방법 : 최근 5년 동안 본원에서 치료한 환자 27례를 대상으로 하였으며 추시 기간은 12개월에서 62개월로 평균 37개월 이였다. 남자가 19례(70.4%), 여자가 8례(29.6%)였으며, 연령 분포는 24세에서 78세로 평균 53세였다. 골절의 분류로는 Schatzker 분류법으로 하였으며 제 Ⅰ 형 3례(11.1%), Ⅱ형 1례(3.7%), Ⅲ형 0례(0%), Ⅳ형 3례(11.1%). Ⅴ형 1례(3.7%) 그리고 Ⅵ형 19례로 Ⅵ형이 가장 많은 빈도를 나타냈다. 27례 모든 환자에서 수술적 치료를 시행하였으며, 필요한 경우 골 결손부에 골 이식술을 병행하였다. 치료 결과는 Blokker의 판정기준으로 평가하였다. 결 과 : 관혈적 정복의 유지를 위한 금속 내고정물로는 나사못 사용이 4례(14.8%), 금속판 사용이 23례*85.2%)였고 이중 제 Ⅵ형 10례(27.0%)에서 골 이식술을 시행하였다. 술 후 합병증은 총 27례 환자 중 10례 환자에서 발생하여 37%를 나타내었으며 운동장애가 3례, 각 형성이 3례, 감염은 3례에서 발생하였고 그 외 외상성 관절염 소견으로 인한 동통을 보인 경우가 1례에서 있었다. Blokker의 판정기준으로 평가하여 22례(81.5%)에서 양호한 결과를 얻었다. 결 론 : 근위 경골과 골절의 수술적 치료시 골절부의 연부조직이 허용하는 한 해부학적 정복은 물론이며 금속판 및 나사못을 이용한 견고한 내고정을 실시하는 것이 술 후 조기 관절운동을 가능하게 하고, 합병증의 가능성을 낮출 수 있을 것으로 사료된다. Introduction : The purpose of this study was to analyze the results, prognosis and complications in the treatment of proximal tibia plateau fractures, and to suggest the guideline for the proper management in the difficult cases of tibial plateau fracture. Materials and Methods : We have analyzed 27 cases, which surgically treated during recent five years with average 36.6 months follow-up. Patients ranged in age from 24 to 83 years at the time of hospitalization, consisting of 19 males(70.4%) and 8 females(29.6%). The type of fracture by Schtzker classification revealed in type Ⅰ 3cases(11.1%), type Ⅱ 1 case(3.7%), type Ⅲ 0 case(0%), type Ⅳ 3 cases (11.1%), type Ⅴ 1 case(3.7%) and type Ⅵ 19 cases(70.4%). The associated injury occurred in 22 cases(81.5%), most of them were ipsilateral fibular, ipsilateral femoral and radioulnar fractures. The results were evaluated by Blokker's criteria. Results : Screw fixation was done in 4 cases(18%) and plate fixation in 23 cases(85.2%), and bone grafting was done in 10 cases(37.0%). There were 10 postoperative complications with 3 cases of knee ankyosis, 3 cases of angular deformity, 3 cases of infection, and 1 case of traumatic arthritis. According to Blokker's criteria, 22 cases(81.5%) had satisfactory results. Conclusions : Accurate anatomical reduction and rigid internal fixation of the proximal tibial plateau fracture enabled early motion and normalization of injured soft tissues, and also provided functional improvement of the knee.

韓牛 種牡牛의 體重에 對한 環境要因의 影響

宋柱葉,白東勳,崔淏盛 全北大學校 1996 論文集 Vol.42 No.-

This study was carried out to investigate overall performance and to estimate effects of the environmental factors on body weights of the bull in Korean Native Cattle. Data used for this study included a total of 545 heads of Korean Native Cattle recorded from 1981 to 1990 at Alpine Experiment Station located in Kangwon province. The effects of year of birth, age of dam, parity of dam and season of birth were estimated by means of least squares method. Overall least squares means and standard errors for the body weights of the bull at birth, 4, 6, 12 and 18 months of ate were 26.8±0.13, 99.3±0.58, 142.4±0.80, 338.0±1.34 and 484.2±2.00㎏, respectively. The effects of year of birth showed significant differences on birth weigh and highly significant differences on the other traits. The effects of age of dam showed significant differences on 4 months of age. The effects of season of birth showed significant differences on all of the traits. the calves born in winter or spring were heavier than those born in summer or fall.

외측 도달법을 이용한 종골 관절내 골절의 관혈적 정복

송경진,양근호,이주홍 대한골절학회 2002 대한골절학회지 Vol.15 No.4

목적 : 관절내 종골 골절에 대해 외측 도달법을 이용한 내고정을 시행 후 치료결과를 분석하여 그 유용성을 확인해보고자 하였다. 대상 및 방법 : 관절내 골절을 가지고 종골 골절의 경우 중 외측 도달법으로 내고정술을 시행하고 1년이상 추시 관찰이 가능했던 20명(21예)를 대상으로 하였다. 성별은 남자 17명, 여자 3명이었고 평균 33세(16~62세)이었으며, 원인별로는 추락사고가 14예이었고 나머지는 교통사고로 인한 골절이었다. 골절의 분류는 단순 방사선 사진상 Essex-Lopresti 분류를 이용하였고, 모든 예는 관절내 골절을 가지는 경우만 포함하였으며 이 중 관절 함몰형이 17예로 대부분이었고, 4례의 설상형은 심한 분쇄골절 양삼이었다. 술 전과 술 후 Bo¨hler 각의 변화를 비교하였고, 임상적 평가는 Paley와 Hall에 의한 평가 기준을 따랐다. 결과 : 수술 전 Bo¨hler 각은 평균 2.8에서 술 후 평균 25.1로 회복 되었고, 임상적 평가 상 우수 3예, 양호 13예, 보통 4예, 불량 1예이었다. 술 후 합병증으로 3예의 창상 열개, 1예의 감염, 1예의 비복신경 손상이 있었으먀, 추 후 합병증으로 일상 생활에 지장을 초래하는 정도의 족관절 운동범위 제한이 3예, 지속되는 동통이 3예, 외상성 관절염이 2예에서 확인되었다. 이 중 1 예에서 운동범위 제한과 동통이, 1예에서 운동제한과 외상성 관절염이 동시에 존재하였다. 1예는 창상 조직의 감염과 이에 따른 괴사로 자유 피판술을 시행하였다. 결론 : 분쇄가 심한 종골의 관절내 골절에서 외측 도달법은 거골하 관절을 직접 노출시켜 정확한 해부학적 복원과 견고한 내고정을 시행할 수 있고 신경 혈관 손상이 적어 유용한 방법으로 사료된다. Purpose : This study was designed to investigate the usefulness of lateral approach for accurate reduction and rigid internal fixation comminuted intra-articular fracture of calcaneus. Meterial and Method : Twenty patients(21 cases) who had inter-articular fracture of calcaneus and underwent an open reduction and internal fixation using lateral approach were enrolled. Using Essex-Lopresti classification, all cases were intra articular fracture, which joint depression type was 17 case and 4 of severe comminuted tongue type. We compared the preoperative and postoperative change of Bo¨hler’s angle and clinical results were analyzed using Paley and Halls evaluation protocol and scoring system. Results : The average Bo¨hler’s angle was restored from 2.8 to 25.1 after operations and clinical results classified 4 well, 12 good, 4 fair, 1 poor cases. Postoperative complications were 2 cases of wound dehiscence, 1 of infection and one had sural nerve injury. Late complocations included 3 cases of limitation of motion of ankle that disturb usual activity, 3 of sustained pain and 2 of traumatic arthritis and 2 cases had 2 complications at the same patients. Conclusion : The lateral approach is valuable for the comminuted intra-articular fractures of calcaneus that enables accurate anatomical reduction and rigid internal fixation by providing direct exposure of subtalar joint, and also with little morbidity of neurovascular injury.

전공의들의 직업적 유해인자 및 직업만족도

송혜란,김진하,변주현,이광영,이덕희,김호찬 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.3

목적: 전공의는 여러 유해인자에 노출되어지고 있으며 과중한 업무로 인한 수면 부족에 시달리고 있다. 본 연구는 전공의의 건강상태의 기초 자료를 제공하고 향후개선 방안을 도출하기 위하여 시행하였다. 방법: 연구대상은 2000년 3사관학교와 국군군의학교에 입소한 전체 1,316명 중 전공의 수련과정 없이 입대한 의사와 치과의사, 한의사를 제외한 881명을 대상으로 설문지를 배포하여 784부(회수율: 89.0%)를 회수하고 이중 분석이 가능한 704(79.9%)명의 자료를 최종 분석하였다. 결과: 월평균 당진횟수는 레지던트 1년차에서 19회 정도이었다. 당직 시 평균 수면시간은 레지던트 1년차에서 4시간미만 이었다. 수련기간 동안 전공의의 20%이상에서 방사선, 레이저, 살균소독제, 마취가스, 항암제에 노출되었으며 예방대책은 방사선과 레이저의 노출의 경우 35%정도를 제외하고는 10%내외로 조사되었다. 연구대상자중 91.1%에서 수련기간 동안 주사침이나 시술에 의해 손상을 입었으며 그 중 본인이 감염된 경우는 HBV 2.3%(4명), HCV 0.6%(1명)으로 조사되었다. 응답자의 28.4%에서 자신의 직업에 불만족 하였으며 96.5%가 과중한 업무부담을 느꼈고 69.5%가 동일한 임금수준에서 직업을 바꾸고자 한다고 응답하였다. 결론: 전공의는 수련기간 동안 물리적, 화학적, 생물학적 유해인자에 노출되고 있었으나 이에 대한 보호나 예방대책은 거의이루어지지 않고 있었으며 과중한 업무부담으로 수면부족을 초래하여 가장 중요한 환자 진료에 지장을 받고 있었다. 수련기간 동안 대다수가 의사로서 직업에 대한 흥미를 가지고 있었으나 업무부담과 진료이외의 업무 등으로 소득과 조건이 비슷하다면 다른 직업을 선택하고자 하였다. 이상의 결과를 토대로 전공의들을 위한 유해인자의 보호대책 및 예방대책을 마련하며 과중예방대책이 마련되어야 하고 업무환경개선 및 업무로 인한 건강장해에 대한 정당한 보상이 향후에 마련되어야 할 것이다. Objects: Residents are exposed to various hazards and harassed by sleep dificiency due to overwork. This study was performed to outline the health condition of residents and develop the ways of improving it. Mehtods: A questionnaire was distributed to 881 army doctors who enrolled at the Korea Third Military Academy and The Armed force Health Service School in 2000 with the exception of medical doctors who joined the armed service without going through intern and resident periods, dental and oriental medical doctors. 784(89.0%) of the 881 questionnaires were resident of which 704 cases (79.9%). This should actually be 89.0% were regarded for analyses as being reliable data. Results: More than 20% of residents were exposed to radiation, laser, disinfectants, anesthetic gas, and anticancer drugs during their training periods with preventive neasures for each of these hazards taken in about 10% of the cases of the lesser hazards but in about 35% for the hazards from radiation and laser. 91.1% of residents had experience of needlestick injuries during theirtraining periods with over 36.3% being exposed to patients with infection during that time. The cases contracting HBV and HCV diseases due to pricking are 2.3% and 0.6% respectively. The average number of night duties per month during a one-year resident's period was about 10. The average sleeping time on duty during a one-year residents was less than 4 hours. 28.4% of respondents were not satisfied with their occupation, 96.5% delt tired from overwork and 69.5% wanted to change their occupation to another that would pay the same salary. Conclusion: Residents are exposed to harmful physical, chemical and biological factors, but the preventive care seldom undertaken. In addition, their overwork brings about lack of sleep which causes them problems when giving medical treatment to patients during their training periods. Despite the resident's interest in an occupation as a doctor, many intend to select other occupations if they can receive the same salary. According to the above results, preventative systems and measures should be prepared with environmental improvements for residents, and proper rewards for their overwork should be accomplished

대망에 발생한 방선균증 1례

송상훈,심찬섭,이문성,김진오,조주영,김영석,문종호,이진우 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

Actinomycosis is an uncommon chronic suppurative disease characterized by multiple abscess, draining sinuses, abundant granulation, and dense fibrous tissue, caused by the actinomyces organisms. It occurs in three major sites: cervicofacial, abdominal, and thoracic. The abdomen is the second most common involved site in previous studies, and there are three ways this organism can affect the gastrointestinal tract: through a blood-borne infection, by swallowing, or by proliferation at a more distal intestinal area. Abdominal actinomycosis is an unusual disease but remains an important differential diagnosis in patients with abdominal mass. Obtaining the diagnosis is difficult and rarely made preoperatively, but provide the key to its management. Medical therapy is effetive in eradicating the disease; however, because of the large amount of reactive fibrosis formed by the infection, it requires long-term therapy. Surgical treatment may be essential in many cases, primarily when malignancy cannot be excluded or if extensive necrotic tissue exists. There are a few description for actinomycosis involving omentum. We report a case of patient with omental actinomycosis, suspected to have a malignancy requiring an exploratory laparotomy with resection.

工業敎育大學 敎育改善에 관한 硏究(Ⅲ)

宋周鎬,李昌岬,洪元杓,孟琦錫,任洪彬,李廷玟,李載元,李來烈,權五憲,金振淳,李文洙 충남대학교 공업교육연구소 1982 論文集 Vol.5 No.1

This paper aims at providing a concrete plan to improve technical and vocational teacher education in the College of Industrial Education of Chungnam National University. It is intended to propose a practical four-year regular and short-term course. Also, the estimated required facilities is included in this study as well as their management. The main conclusions and recommendations are summarized as follows; 1) The numbers of students enrollment should be reduced to 220 students which is the approximate number of future demand. 2) In order to offer to the students a broader education and to cope with the increase/decrease in demand of technical and vocational teacher within a specific field, the 8 department existing at the present (Department of Architecture Engineering Education, Civil Eng. Educ., Metallurgical Eng. Educ., Mechanical Eng. .Educ., Electrical Eng. Educ., Electronic Eng. Educ., Industrial Chemistry Educ. and Industrial Technology Educ.) would be combined into 5 (Department of Construction Engineering Education, Mechanical Eng. Educ., Electrical Eng. Educ., Industrial Chemistry Educ. and Industrial Technology Educ.). 3) The short-term course program is recommened to provide additional training and education to those candidates who already have experience in technical fields to meet an occasional demand. 4) It is necessary to add the Teaching Profession Section in the administrative organization of the College.