사매가 Ovalbumin감작으로 유발된 천식 생쥐 모델에서 기관지염증 억제에 미치는 영향

임중근,노성수,길기정,이영철,서영배 대한본초학회 2005 大韓本草學會誌 Vol.20 No.1

Asthma is a chronic inflammatory disease of the airways, characterized by airway eosinophilia, goblet cell hyperplasia with mucus hypersecretion, and hyper-responsiveness to both inhaled allergens and nonspecific stimuli. Eosinophil are important in the pathogenesis of asthma, with release of inflammatory mediators such as histamine, leukotrienes, cytokines, and IgE after exposure to allergen. Objectives : This research investigates the effect of the extract of Duchesnea chrysanta (EDC) on OVA-indued asthma murine model. Methods : After i.p of C57BL/6 mice, OVA sensitization (day 0) were challenged intratracheally with OVA on days 8 and 21. EDC administered weeks 8, blocked the airway inflammatory response to OVA assessed 24 h after the last OVA challenge on day 22. Results : The CC chemokine receptor 3 (CCR3) is expressed by eosinophils, mast cells, and Th2 cells. The role of CCR3 in a murine model of allergic skin inflammation induced by repeated epicutaneous sensitization with ovalbumin (OVA), and characterized by eosinophil skin infiltration, local expression of Th2 cytokines, and airway hyperresponsiveness (AHR) to inhaled antigen. The administration of EDC during OVA inhalation significantly reduced CCR3 plays an essential role in eosinophil recruitment to the skin and the lung and in the development of AHR.. EDC extract reduced total lung cells and eosinophils in bronchoalveolar lavage fluid, airway tissue eosinophilia, goblet cell hyperplasia, mucus secretion, and peribronchial edema. The inhibitory effect on airway eosinophilia and AHR was associated with reduced levels of IL-4, IL-5, and IL-13 in the bronchoalveolar lavage fluid as well as reduced serum levels of OVA-IgE. We show that OVA inhalation following OVA immunization increased airway responsiveness to induced IL-113, IL-4 and IL-5 mRNA expression in the lung tissue. The administration of EDC during OVA inhalation significantly reduced this increased airway hyperresponsiveness and also inhibited airway inflammation. Conclusions : These results support EDC as a therapeutic target in asthma and indicate that selective EDC inhibitors can reduce allergic airway inflammation.

Risperidone 약물치료가 정신분열병 환자의 주의력 및 정보처리 기능에 미치는 영향

우종민,강동우,김이영,김도관,서만길,손성은,홍경수 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5

연구목적 : 비정형 항전신병약물의 약물 역동학적 특성은 정신분열병 환자의 양성, 음성 증상 뿐 아니라 인지기능에도 긍정적 효과를 미칠 것으로 기대된다. 본 연구의 목적은 정신분열병 환자에서 8주간의 risperidone 약물치료가 주의력과 정보처리력에 미치는 영향을 파악하고, 이것이 임상 증상의 변화와 연관되어 있는지 여부를 알아보는 것이다. 방 법 : 연구 대상은 정신병적 증상의 발현 혹은 악화 상태에 있는 29명의 정신분열병 환자와 정상 대조군 25명이었다. 임상 증상은 치료 전후로 Positive and Negative Syndrome Scale, Clinical Global Impression 및 Extrapyramidal Symptom Rating Scale을 사용하여 평가하였다. 치료 전후의 환자군과 대조군에 대해 전산화 인지기능 검사인 Vigilance Test, Continuous Attention Test, Reaction Unit Tests, Motor Performance Tests를 시행하여 경계력(vigilance), 지속적 주의력, 시각/청각 자극에 대한 정보처리력 및 미세운동 조절능력을 평가하였다. 결 과 : 치료전 환자군은 경계력과 지속적 주의력, 미세운동 조절능력에서 대조군에 비해 유의하게 저하된 성적을 보였으며, 시각/청각 자극에 대한 반응 결정속도도 저하된 경향을 보였다. 8주간의 risperidone 약물치료 후 임상 증상은 양성척도, 음성척도 및 일반정신병리척도에서 모두 유의한 호전을 보였다. 치료 후 환자군은 시청각 복합 자극에 대한 반응 결정 속도의 유의한 향상을 보였는데, 이는 임상 증상의 호전 정도와 상관성을 보이지 않았다. 단순 자극에 대한 반응결정 속도, 주의력, 미세운동 조절기능 등은 치료 전후로 차이를 보이지 않았고, Vigilance Test의 반응시간은 지연되었다. 치료 후 추체외로 증상 정도와 상관성을 보이는 인지기능 검사 항목은 없었다. 결 론 : 정신분열병 환자는 경계력, 지속적 주의력 및 미세운동 조절기능의 결함을 보이며, 이들 결함은 risperidone 약물치료로 임상증상이 호전된 후에도 비슷한 수준으로 유지되는바, 질병 고유의 특성임이 시사되었다. 치료 후 시청각 복합자극에 대한 반응 결정속도가 향상되었는데, 이는 임상 증상 호전과는 무관한 약물 역동학적 기전에 의한 직접적 효과임이 시사된다. Objectives : The aim of this study is to evaluate the effect of risperidone on attention and information-processing in schizophrenic patients after 8 weeks of treatment and to determine whether any improvement on cognitive function relates to improvement in psychopathology. Methods : The subjects were 29 schizophrenic inpatients with active psychotic symptoms and the controls were 25 normal volunteers. Pre- and post-treatment clinical symptoms were assessed by Positive and Negative Syndrome Scale, Clinical Global Impression and Extrapyramidal Symptom Rating Scale. Vigilance, continuous attention, the speed of information-processing and fine motor coordinations were assessed by standardized computerized neurocognitive function tests. Results : At baseline, patients showed significantly poor vigilance and continuous attention than normal controls and these functions were not improved after treatment. The positive and negative symptoms and the general psychopathology were significantly improved. The only item of cognitive tasks significantly improved after treatment was decision time to complex audio-visual stimuli in information-processing task. This improvement was not related to the improvements in psychopathology. The level of extrapyramidal symptoms was not related with any of the cognitive function tests items. Conclusions : Treatment with risperidone appeared to exert a favorable effect on the speed of information-processing and this appeared to be due to a direct pharmacodynamic characteristics of the drug.

EFG와 IGF-I의 첨가배양이 돼지 미성숙 난포란의 체외성숙과 배발달에 미치는 영향

백준종,한만희,박병권,서길응,이규승 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.1

The present study was carried out to examine the effect of EGF and IGF-I in vitro maturation (IVM) of porcine oocytes and development of porcine IVM/IVF embryos. The results were summarized as follows : 1. The rates of nuclear maturation, penetrated oocytes, pronuclear formation, polyspermic oocytes and mean numbers of the penetrated sperm were not different in NCSU-23 maturation medium with 0, 1. 5 and 10 ng/ml EGF and IGF-I (P>0.05). 2. The rates of blastocyst formation at day 7 after in vitro fertilization in 0, 1, 5 and 10 ng/ml EGF groups were 11.2±1.5%, 15.0±8.3%, 16.8±2.8% and 21.4±2.0%, also 0, 1, 5 and 10 ng/ml IGF- I groups were 11.2±1.5%, 15.0±8.3%. 16.8±2.8% and 21.4±2.0%, respectively. In the total cells case, EGF groups were 22.8±3.7, 25.7±5.5, 26.0±4.2 and 35.1±4.7, also IGF-I groups were 21.5±3.7, 25.2±2.8, 26.2±2.9 and 33.2±3.6, respectively. Both 10 ng/ml EGF group and 10 ng/ml IGF-I group were significantly higher than those of other treatment groups (P<0.05). 3. The rates of blastocyst formation at day 7 in the NCSU23 culture medium of porcine IVF-produced embryos with 0, 1, 5, and 10 ng/ml EGF groups were 14.0±1.7%, 16.2±1.4%, 16.9±1.2% and 23.1±1.6%, also 0, 1, 5, 10 ng/ml IGF-I groups were 13.6±1.7, 15.7±4.5. 16.0±0.2 and 25.0±0.8, respectively. And in the total cells case, EGF grups were 21.8±2.9, 25.2+2.8, 39.7±2.7 and 46.2±3.6, also IGF-I groups were 20.7±2.9, 26.2±2.9, 24.6±2.4 and 46.1±3.5, respectively. Both 10 ng/ml EGF group and 10 ng/ml IGF-I group were significantly higher than those of any other treatment groups (P<0.05). In conclusion, these results suggested that the addition of 10 ng/ml EGF and IGF-I were effective on the blastocyst formation and total cells of blastocysts.

북한 이탈주민의 외상후 스트레스장애에 대한 3년 추적연구

홍창형,유정자,조영아,엄진섭,구현지,서승원,안은미,민성길,전우택 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.1

Objective : This study is a follow-up of a first survey of 200 North Korean defectors who entered South Korea in 2001. The purpose of this study is to examine changes in the current prevalence of partial PTSD and full PTSD after 3 years and to evaluate related factors. Methods : This study followed up on 151 of the 200 North Korean defectors from our first survey. 20 interviewers conducted face-to-face interviews and assisted defectors in completing a self-report assessment. The traumatic events which participants with chronic PTSD and delayed onset PTSD experienced were then examined. Results : Over the 3 years, the current prevalence rate of partial PTSD was reduced from 31.8% to 5.3%, and the current prevalence rate of full PTSD was reduced from 27.2% to 4.0%. Of particular interest was that 88.8% of those diagnosed with full or partial PTSD in the first survey had recovered after 3 years. Conclusion : The current prevalence rate of partial and full PTSD rapidly decreased during 3 years.

Lipidized Fibrous Histiocytoma: Differential Diagnosis from Juvenile Xanthogranuloma

( Jong-kil Seo ),( Eun-jae Shin ),( Ki-heon Jeong ),( Min Kyung Shin ) 대한피부과학회 2019 Annals of Dermatology Vol.31 No.2

Skin necrosis following accidental injection of sodium bicarbonate

( Jong-kil Seo ),( Ye-jin Lee ),( In-hye Kang ),( Hye-jin Ahn ),( Ki-heon Jeong ),( Min Kyung Shin ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Intravenous sodium bicarbonate injection has been used to treat metabolic acidosis. When it was used in low amount of fluid, no common complications have been reported. It is a component with little potential for intradermal injection and rarely reported adverse effects of skin injection. Moreover, the local adverse effect resulting in a skin necrosis similar to burn is very rare. A 20-year-old female presented with a painful ulcer on the right forearm. She was a nursing student and was burned due to injection of sodium bicarbonate accidentally during intradermal injection practice. Physical examination revealed an erythematous, ulcerative patch that was 1.5 x 1 cm in diameter. We initially treated with simple dressing for 2 weeks, but lesions soon became necrotic. Therefore, the treatment with collagen sheets and EGF solution was performed for one month. And the skin lesion was re-epithelialized. Since sodium bicarbonate barely has an event for intradermal injection, we herein report a rare case of skin necrosis following an intradermal infusion of sodium bicarbonate for educational purposes.

Safety and clinical outcomes of the 1064 nm Neodymium-doped Yttrium aluminum garnet (Nd:YAG) laser for onychomycosis in patients with diabetes mellitus

( Jong-kil Seo ),( Hye-jin Ahn ),( Hyung-jin Park ),( Ye-jin Lee ),( Mu-hyoung Lee ),( Min Kyung Shin ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Onychomycosis(OM) affects approximately one third of patients with diabetes. But, a treatment failure risk of oral antifungal agents in diabetic patients is high due to poor patient compliance, drug interactions and decreased immune status. The Neodymium-doped yttrium aluminum garnet (Nd:YAG) laser has recently been widely used as one of therapeutic options for OM. However, there have been few studies on clinical outcome of 1064nm Nd:YAG laser treatment in diabetic patients. Objectives: The aim of our study was to evaluate the outcome and safety of 1064 nm Nd:YAG laser treatment for OM with diabetes mellitus (DM). Methods: We performed a retrospective chart review to collect data such as sex, age, Hemoglobin A1c, clinical photo and adverse effects. Nails with OM were treated by 1064 nm Nd:YAG laser with 4 to 6 sessions at 4 weeks interval. We assessed onychomycosis severity index (OSI) score and compared outcomes and safety in three groups; controlled DM, uncontrolled DM, and healthy control. Results: There was no adverse effect after the treatment in 3 groups. The response rate was not significantly different between controlled DM group and healthy control group (p=0.128). But, the response rate in uncontrolled DM group was significantly lower than other groups. Conclusion: The 1064nm Nd:YAG laser may be a safe treatment option for OM in diabetic patients. And proper regulation of blood glucose levels in diabetic patients may be needed to improve the therapeutic effect for OM.

A case of dermal infection of Trichophyton rubrum in an immunocompetent patient

( Jong-kil Seo ),( Ye-jin Lee ),( In-hye Kang ),( Hye-jin Ahn ),( Min Kyung Shin ),( Jong Soo Choi ),( Mu-hyoung Lee ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Dermatophytosis is a common skin disease affecting the surface of the skin, including stratum corneum, hair and nails. It is also possible for dermatophytes to invade the dermis and subcutaneous fat layer, especially in immunocompromised patients. A 78-year-old female presented with localized coin-sized erythematous swollen patches with central ulcer on the left lower leg. She had hypertension and angina, and no other illnesses that could reduce her immune system. The histopathological evaluation demonstrated ill-demarcated, non-encapsulated granulomatous inflammation composed of multinucleated giant cells, lymphohistiocytes and neutrophils in the deep dermis and subcutaneous fat layer. Additional stains revealed many acute angle branching hypae (Periodic Acid-Schiff and Gomori Methenamin-Silver) in granulation tissues. Trichophyton rubrum was identified by REBA Fungus-ID^® in biopsy tissue. Herein we report a rare case of dermal infection of Trichophyton rubrum in an immunocompetent patient.

Eccrine poroma arising within nevus sebaceous

( Jong-kil Seo ),( Hyo-eun Kwon ),( Ye-jin Lee ),( In-hye Kang ),( Ki-heon Jeong ),( Mu-hyoung Lee ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

Secondary neoplasms in nevus sebaceous can develop during adolescence and adulthood. Trichoblastoma and syringocystadenoma papilliferum are the most common benign neoplasms, but poroma is rarely reported. A 28-year-old female presented with an asymptomatic mass on the scalp. She have had a hairless lesion on the scalp since birth. A soft mass developed on that lesion four years prior. Physical examination revealed a localized 1x2.5 cm-sized brownish, verrucous-surfaced plaque with a 1x1 cm-sized pedunculated erythematous tumor on the scalp. We performed skin biopsy on both the plaque and tumor lesions. The histopathological findings demonstrated the plaque lesion consistent with nevus sebaceous and the tumor lesion consistent with eccrine poroma. Surgical mass excision was performed. The patient was eventually diagnosed with eccrine poroma arising within nevus sebaceous. To the best of our knowledge, there are only six reported cases on poroma arising within nevus sebceous. Although rarely documented in the literature, it should be considered as a secondary neoplasm within nevus sebaceous.

Generalized eruptive diabetic dermopathy for type 2 diabetes patient

( Jong-kil Seo ),( Ye-jin Lee ),( In-hye Kang ),( Hye-jin Ahn ),( Mu-hyoung Lee ),( Min Kyung Shin ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Diabetic dermopathy is the most common cutaneous change in patients with long standing diabetes. It generally presents as well-demarcated, hyperpigmented erythematous to brownish, and flat to atrophic skin lesions located on the anterior surface of the lower legs. Also, it often occurs on forearms, thighs, and the side of feet. But it is rarely reported with generalized eruption. A 47-year-old male presented with asymptomatic skin eruptions that developed suddenly 6 months ago. He had been diagnosed with hypertension, type 2 diabetes mellitus, and hyperlipidemia 7 years ago. A year prior to presentation, diabetes was not under control. He was diagnosed with diabetic dermopathy based on the clinicopathological findings which revealed well-demarcated brownish maculopapules on both elbows, legs and trunk, and biopsy findings which showed scattered melanin and hemosiderin deposit in the upper dermis. We report a rare case of diabetic dermopathy with generalized eruption.