독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅱ) : 망간 신경독성을 이용한 파킨슨증 모델의 세포사 Studies on the Mechanism of Parkinsonism induced by Manganese

김종민,박창원,오정자,이보경,서경원,서수경,김규봉,김종원,김광진,김영옥,전범석,박찬웅,이선희 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

1960년대 이후 파킨슨병의 유발물질 중 한 후보로서 망간이 주목받으면서 많은 역학적, 병러학적 연구가 수행죄브다. 그러나 망간이 파킨슨병을 직접 일으키는지, 혹은 파킨슨병과 관련된 부위의 신경세포를 파괴시쿡 파킨슨증만을 초래하는 것인지가 아직 정립되지 않은 실정이다 본 연구에서는 실험동물모델에서 행등학적 변화 측정 및 병리학적, 샐화학적 연구를 통하여 망간의 과다노출글 따른 파킨슨병 유발 여부를 확인하고자 하였다. Sprague-Datylet· 렛드에 망간을 1, 2, 5, 10 mgAg/day의 응량으로 30일 등안 복강 투여하였을 때 모든 망간투여군에서 운동성 감소가 관찰되었다. 뇌조직의 망간 함량을 ion chromatograph?로 측정한 결과 중뇌 흑질과 기저핵 선조체에서 망간 함량의 뚜렷한 증가 소견이 관찰되었으며, 니슬염색체서 선조체의 신경세포수의 유의성 있는 감소가 관찰되었다. 그러나, 흑질의 TH효소 면역염색, GFAP 면역염색, 흑질과 선조체의 T릿효소 western blot 결과는 대조군과 차이가 없었다. 이상의 결과에서, 망간-파킨슨증 모델에서 파킨슨증어 유발되는 기전은 흑질 도파민성 신경 세포의 신호를 받는 선조체 씬경세포의 사멸에 의한 것으로 생각된다. 결론적으로, 망간 독성에 의한 파킨스증 모델에서는 파킨슨 병과는 달리 흑질 도파민성 신경세포의 세포사는 관여하지 않는 것으로 관찰되었다. Manganese(Mn) intoxication causes a parkinsonian syndrome. It may be difficult to distinguish Mn-induced parkinsonism from idiopathic Parkinson disease(IPD). Neuropathological descriptions on the brains with Mn intoxication showed the perferential damage in the globus pallidus and substantia nigra pars reticularis. Pathological reports on the substantia nigra pars compacta(SNpc), a mafor focus of pathologic changes in IPD, are discordant and controversial. The SNpc involvement is of critical importance for the elucidation of pathogenesis of IPD. Therefore, the neurodegeneration in SNpc was investigated in the experimental model of Mn neurotoxicity. Sprague-Dawley rats were administered with manganese chloride(1, 2, 5, 10 mg/kg/day, i.p.) for 30 days. Treated animals showed low levels of distance-traveled from locomotor activity tests. Ion chromatography revealed that Mn accumulation was significant in SN and basal ganglia in Mn-treated animals. Nissl staining showed neuronal loss of the striatum in all treated animals. The degree of neurodegeneration in SN was determined by immunohistochemical staining of tyrosine hydroxylase(TH). The numbers of TH-positive cells on nigral sections were not different from each groups(P>0.05). The densities of glial fibrillary acidic protein immunoreactivity from SN were similar between groups. TH-westen blotting showed no differences between treated animals and controls. In conclusion, the SNpc remains intact in Mn intoxication, and Mn-induced parkinsonism may be caused by damages of output pathways downstream to the nigrostriatal dopminergic system.

하악골에 발생한 골막 골육종

김태우,김승범,권혁찬,문선재,윤정훈,김형준,차인호,육종인,김진 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

Periosteal osteosarcoma is a distinct entity of malignant bone tumor with characteristic clinical, morphological, and histological features within the group of juxtacortical osteosarcoma. Periosteal osteosarcoma is predominantly located in the tubular long bones, and extremely rarely involving the jaws. A case of periosteal osteosarcoma of the right mandible is presented. A 27-year-old woman complained of the gingival swelling and bleeding tendency of the right posterior mandible. Clinical examination revealed a reddish brown strawberry-like swelling on the affected mucosa, which measured 1.5㎝×1.5㎝. The tumor was located on the lingual cortex of the mandible and extended into the surrounding soft tissues. Microscopically, the tumor consisted exclusively of atypical chondroblastic cells with a small osteoblastic area. A minimal bone marrow involvement was noted and the adjacent cortex was free of tumor. These findings suggested that the tumor originated from the periosteal cambium layer, which lies between the periosteal fibrous layer and the cortex of mandible.

CD34+ 조혈 모세포 이식 2례

김정아,정현식,김원석,윤성수,이홍기,박찬형,박성규,김동욱,이종욱,한치화,민우성,김춘추,김동집 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

Background: In most solid tumors, the CD34 antigen has not been detected, so positive selection of CD34+ cells may reduce tumor cell contamination and the CD34+ cells are capable of reconstituting hematopoiesis. We tried CD34+ cell transplantation in two patients. Method: CD34+ cells from chemotherapy + G-CSF mobilized PBPCs or bone marrow were positively selected with an avidin-biotin immunoadsorption column (CEPRATE SC system). Case 1. One course of chemotherapy using cyclophosphamide(200㎎/㎡) and etoposide (4.2g/㎡), combined with G-CSF(5㎍/㎏) S.C. was used in a relapsed lymphoma patient. This patient responded to the induction chemotherapy. CD34+ cells from harvested bone marrow were selected by the CellPro immunoadsorption column. The total number of mononuclear cells loaded onto the CellPro was 2.4×10^(8)/㎏, with 1.1% CD34+ cells. After column separation, the total number of positively selected cells was 5.16×10^(6)/㎏. The number of CFU-GM was 76.8×10⁴/㎏. This patient was treated with melphalan (140㎎/㎡) and TBI (1200cGy) and the positively selected CD34+ cells were infused. The time to neutrophil recovery greater than 0.5×10^(9)/L was 19 days and the time to platelet recovery greater than 50×10^(9)/L was 21 days. Case 2. Two courses of mobilizing chemotherapy were given 4 weeks apart using taxol(210㎎/m2) and adriamycin(60㎎/m2), combined with G-CSF(5㎍/㎏) S.C. in a breast cancer patients with 7 axillary node metastasis. CD34+ cells from each single leukapheresis product were selected by the CellPro immunoadsorption column. In the first collection, the total number of nucleated cells was 4.4×10^(8)/kg, with 0.42% CD34+ cells. In the second collection, the total number of nucleated cell was 2.8× 10^(8)/㎏ with 0.43% CD34+ cells. After colum separation, the total numbers of collected cells were 4.0×106/kg and 4.8×10^(6)/kg, the total number of CD34+ cells were 1.2×10^(6)/㎏ and 0.82×10^(6)/㎏. Colonogenic assays of positively selected CD34+ cells gave rise to myeloid erythroid, and multilineage colonies, with a median of 190 CFU-GM, 190 BFU-E, and 164 CFU-GEMM per 1×10³ adsorbed cells, respectively. High-dose chemotherapy with cumulative doses of 40mg/㎡ mitoxantrone, 750mg/㎡ thioptepa, and 1000mg/㎡ carboplatin was administered. Positively selected CD34+ cells were rapidly infused 24 hours after the end of high-dose chemotherapy. The time to neutrophil recovery greater than 0.5×10^(9)/L was 16 days and the time to platelet recovery greater than 50×10^(9)/L was 20 days.

위장관 출혈을 일으킨 공장의 형활근종 1예

김병수,장성종,부귀범,조운택,박찬국,김만우,변주남,김정용,전호종 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Small bowel leiomyoma is a rare tumor which may occur wherever smooth muscle is present. The small bowel is the most common site and it makes up 20~30% of all benign small intestinal neoplasms. A spectrum of symptoms are common ranging from nausea, vomiting, abdominal pain, to massive intestinal bleeding. Bleeding is the most common symptom and is usually gross and recurrent. Occasionally it may be life threatening. But, the most serious problem is both the absence of specific clinical symptoms, and the difficulties shown in radiological diagnostics of the small bowel. If conventional diagnostic methods fail to reveal any positive findings for gastrointestinal bleeding, the diagnosis of intestinal leiomyoma should be suspected and an SMA angiography shoud be performed. The angiographic criteria of small bowel leiomyoma is very characteristic. We present a case of leiomyoma with considerable and prolonged gastrointestinal bleeding, which was diagnosed by angiography. A 61-year old woman was admitted with a history of melena over the last 10 days. An SMA angiography showed a hypervascular mass with a diameter of 3×3cm. A segmental resection was performed under the impression of the gastrointestinal bleeding due to leiomyoma of the small bowel. Microscopic examination of the lesion shows interlacing or whorl bundles of spindle shaped ces and no mitosis.

중증 재생불량성 빈혈 환자에서 신우신염에 대한 광범위항생제치료 중에 속발한 Saccharomyces cerevisiae 진균감염 1예

김철희,이정호,이정찬,강정현,곽상혁,배광봉,김현수,김종숙,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

Most patients with aplastic anemia who do not respond to immunosuppressive treatment or are not candidates for bone marrow transplantation die of infection or bleeding. The neutropenia in acute leukemia, aplastic anemia, or occurring subsequently to chemotherapy and bone marrow transplantation increases susceptibility to infection. In general, the number of infectious episodes correlate with the degree and duration of neutropenia. Global immunosuppression produced by conditioning for bone marrow transplantation or graft-versus-host disease, is associated with unusual bacterial and fungal pathogens, or serious viral and protozoan infections. In addition, repeated treatment with broad-spectrum antibiotics is associated with the emergence of resistant organisms and fungal diseases because of the altered microbial microenvironment of the host. The incidence of invasive fungal infection caused by Saccharomycetes eerevisiae in immunosuppressed patients is very rare, compared with that of infection by candida or aspcrgillus species. Cases of Saccharomycetes cerevisiae fungemia occurring in the course of treatment with broad-spectrum antibiotics are reported in patients with extensive burn or with prosthetic valve endocarditis. We experienced a case of urinary tract infection by Saccharomycetes cerevisiae in a 27-year old female patient with severe aplastic anemia. We report the case with a review of relevant literatures.

단기간의 저용량 Cytosine arabinoside 치료에 반응하였던 Down 증후군에 병발한 급성골수성백혈병 1례

김현수,이정호,이정찬,강정현,곽상혁,김철희,배광봉,김종숙,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The incidence of hematologic disorders in patients with Down's syndrome is significantly increased, about 14∼30 - fold higher than in general population and includes neonatal transient abnormal myelopoieis and acute leukemias. The age of onset of leukemia in Down's syndrome is peaking first in the newborn period and then under 4 years of age. Down's syndrome with acute leukemia above the age of 20 is very rare and it's treatment oucome is unclear. The treatment of Down's syndrome with leukemia has been controversial because of toxicity and associated congenital cardiac and other abnormalities. But if treated adequately, children with Down's syndrome show a favorable response to anti-leukemia therapy. A 24-year-old man with Down's syndrome was first seen for the evaluation of anemia and thrombocytopenia. The peripheral blood morphology and bone marrow study revealed acute myelogenous leukemia, cytogenetic study of bone marrow showed trisomy 21. Beacuse of his sicioeconomic condition and medical abnormalities including deafness, visual loss, he was treated with low dose subcutaneous cytosine arabinoside(Ara-C) for 11 days. Complete remission was obtained after 37 days. The complete remission lasted for 5 months. He subsequently relapsed, and died 6 months later.

췌장의 가성낭종 파열에 의한 하부 위장관 출혈 1예

장성종,김병수,부귀범,김동규,박찬국,김만우,김정용,박상헌 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

Pancreatic pseudocysts usually develop as a complication of acute pancreatitis or repeated attacks of chronic pancreatitis. Pseudocysts may also develop as a result of traumatic injuries or neoplasms. An uncommon complication is massive hemorrhage inside the pancreatic pseudocyst. The exact mechanism of hemorrhage is unknown. Enzymatic digestion, pressure erosion from the cyst mass or a combination of these processes may play an important role. Gastrointestinal hemorrhage associated with a pancreatic pseudocyst can occur in 10 percent of cases. When hemorrhage occurs because of erosion into a blood vessel involved in the pseudocyst, surgery is indicated since the episodes of hemorrhage are often severe and may even be fatal. When there is colonic involvement, various complications may occur. The most dangerous one is spontaneous rupture into the colon, which requires immediate surgical treatment because of high incidence of fulminating sepsis or massive hemorrhage. We examined a sixty eight-year-old man complaining of hematochezia and dizziness, and reported that it was a case of massive lower gastrointestinal hemorrhage caused by pseudocyst of the pancreas ruptured into the colon associated with chronic pancreatitis. On operative findings, the peripancreatic fatty tissue had severe adhesions to transverse colon and tail portion of pancreas, where the pseudocyst had formed. A distal pancreatectomy, splenectomy and resection of transverse colon were performed. By microscopic examination, hemorrhagic necrosis and inflammatory change were observed in the pancreas specimen. The tail portion of pancreas had a 6×6 cm sized cystic mass and was proven to be a pseudocyst with no epithelial lining.

동진강 유역 비점오염원 정밀조사

원찬희,정팔진,김민정,조선영,김세훈,김종천 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2002 工學硏究 Vol.33 No.-

Nonpoint source pollution(NSP) arises due to agriculture activity and it is quite an extensive and complicated problem in order to control and has received a great deal of concern during the recent years, whereas point source are known to originate from an estimated volume of contaminant discharged from a distinguishable source. Extensive research work have been conducted and presented in the past with regard to the various characteristics of watershed on Dong Jin River(DJR), however there are certain drawbacks of existing survey for determining important polluting sources, distribution features of pollutants and estimated load of the organic and nutrients from the creeks. Unfortunately, investigation for loadings originating due to agricultural drainage was not satisfactory, although the regional consideration for DJR across Kim Je Field(KJF) as an agricultural area receives a reasonable source point in the agri-pollution management. Therefore it was necessary to carry out a thorough investigation into the diffuse sources resulting, to form the pollution-developing estimation and to evaluate the output roperties and determining various factors in the generation of non-point pollution. Eventually, this research work would serve as an useful tool for the development of predictive pollution management for DJR. Non-point sources in the present work were classified as municipal non-treated sewage water discharged into the river, loadings through agricultural drainages in the dry/wet field and inadequate treatment of wastewater generated from livestocks. The entire duration of the research work was from December-2001 to July-2002. Site measurement were conducted during various season, in a dry season(December), in rainfall, in seed-sowing time and also collecting data from the rural public organizations related to water management was done and examined and examined during the mid of research work. Similarly, domestic wastewater loadings due to population was based on area unit, as City, Country, Up, Myon, Dong, Ri. The Dong Jin River watershed contains small three Creeks, KoBu(KB), DongJin(DJ, including JeongEup(JE) and ChilBo(CB), WonPyong(WP). It was further divided into 32 sub-watersheds which comprises of further 5,18,9 watersheds in the creeks respectively. The results obtained from research work are presented as follows. Animal productions have marked differences in the rate of pollutant loadings reported in the following sequence: DJ(only CE)>WP>CB>KB, in a case of Korean cow and milk cow. However, it was revealed that WP creek became significantly higher than any other livestocks. According to statistical data for land use, rice field(369.0㎢) and forest(378㎢) have much occupation prior to dry paddies(125.0㎢), residential land(32.0㎢) and others(5.0㎢). Especially, rice fields receives 2.5∼3 times than dry paddies in size.

초·중·고 학생들의 과학 탐구 능력 추이 분석을 위한 종단적 연구

우종옥,김범기,허명,김찬종,양일호,최관순,김태선 한국교원대학교 과학교육연구소 1999 청람과학교육연구논총 Vol.9 No.1

본 연구에서는 우리 나라 초·중·고등학교 학생들의 과학 탐구 능력에 대한 종단적 추이를 분석하였다. 1997년에는 우종옥 등(1998)이 개발한 검사지를 사용하였으며, 1999년에는 이를 일부만 수정하여 사용하였다. 조사 대상은 성별, 지역별 등을 고려하여 표집하였다. 조사된 자료는 각각 횡단적으로 분석하고, 이를 바탕으로 학생들의 과학 탐구능력이 어떻게 변해 가는지 종단적 추이를 분석하였다. 조사 결과 초등학생들의 과학 탐구 능력은 유의미한차이가 없었으나, 중·고등학생들의 과학 탐구 능력은 유의미하게 향상된 것으로 나타났다. 학교 과학 교육의 질을 과학적으로 관리하기 위해서는 교육과정이 추구하는 교육 목표의 달성이 성공적으로 이루어지고 있는지를 국가수준에서 주기적으로 측정하여 부진의 원인을 진단하고, 개선 방안을 계속 연구하여야 할 것이다. The longitudinal trend of elementary and secondary students' science process skills were investigated to monitor the progress of science learning. Science process skill test, developedin an earlier study on developing a national science process skills assessment system, was revised and administered for the study on March, 1999. Subjects were sampled nation wide according to sex and region. The results of this assessments were compared to those administered on February, 1997. The results showed little difference on science process skills among elementary school students. However, secondary students showed marked increase in science process skills. More systematic and long-term research for assessing science process skills should be planned and performed periodically to provide empirical and foundational data for educational policy making.

제 3형 Gaucher세병으로 인한 진행성 근간대성 간질 2예

허경,예종수,김상진,김성은,김응규,조효근,김찬환,김현주 대한신경과학회 1999 대한신경과학회지 Vol.17 No.3