샤시부 주요 조인트의 TAY Spec.제정에 이용한 초음파 방식의 패스너 시험

박창언(Changeon Park),김진호(JinhoㆍKim) 한국자동차공학회 2010 한국자동차공학회 학술대회 및 전시회 Vol.2010 No.11

This paper focuses on the fastener test of ultrasonic method applying TAY(Torque and Angle to Yield) specifications for Chassis’s critical joints. By contrast with the existing sensors for measuring tension-compression, Ultrasonic testing method is nondestructive test that is unnecessary to make test sample machined. Due to not machined test samples, the yield strength and prooflaod of bolts doesn’t become weaken. We can measure bolt preload for clevis-type joint by applying the ultrasonic testing method. Therefore, considering the advantages of ultrasonic testing method, it is highly recommended to use this test method for measuring tension data of clevis-type joint. Especially When determining ‘TAY(Torque and angle to yield) specification’ for the clevis-type joints, the ultrasonic method is highly appropriate for measuring the tension of jointed bolts without destroying the unique proof-load/yield strength.

Automatic Frame Composition Using Histogram Based Graph Cut

Daehee Kim,Hyungtae Kim,Jinho Park,Donggyun Kim,Joonki Paik 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.1

In this paper, we present an automatic background composition method using histogram-based graph cut. The proposed method consists of four steps: i) initial label map generation, ii) label map update, iii) object extraction by segmentation, and iv) dynamic background composition. Since the proposed method can minimize the user interaction for generating the initial label map and updating, it is suitable for simple interaction using a low-speed processor and limited memory space. Experimental results show that the proposed method provides better segmentation results compared with existing state-of-the-art methods with significantly reduced computational complexity. The proposed automatic object segmentation and background composition method can be applied to video editing, video conference, and video contents creation using low-cost mobile devices such as smart phones, smart TVs, and tablet PCs.

현대제 170kV GIS DS 조작 Mechanism부 부분방전 발생에 따른 내부점검

김영수(Youngsoo Kim),문병선(Byongseon Moon),김도완(Dowan Kim),한철인(Chulin Han),김대현(Daehyun Kim),김유진(Youjin Kim),김진호(Jinho Kim),이건주(Keonjoo Lee),백동순(Dongsoon Baek),김덕기(Deokgee Kim) 대한전기학회 2022 대한전기학회 학술대회 논문집 Vol.2022 No.7

Is Helicobacter pylori Associated Functional Dyspepsia Correlated With Dysbiosis?

( Yeon-ji Kim ),( Woo Chul Chung ),( Byung Wook Kim ),( Sung Soo Kim ),( Jin Il Kim ),( Na Jin Kim ),( Jinho Yoo ),( Soo Hwan Kim ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2017 Journal of Neurogastroenterology and Motility (JNM Vol.23 No.4

Background/Aims To assess the long-term effect of Helicobacter pylori eradication on symptomatic improvement according to the type of antibiotic and the duration of treatment in H. pylori-associated functional dyspepsia. Methods We searched Pubmed, Embase, CINAHL, and the Cochrane library databases for randomized controlled trials written in English and undertaken up to August 2016 that met our eligibility criteria. The search methodology used combinations of the following keywords: Helicobacter pylori OR H. pylori OR HP; dyspepsia OR functional dyspepsia OR non-ulcer dyspepsia; eradication OR cure OR treatment. The study outcome was the summary odds ratio (OR) for symptomatic improvement in H. pylori-associated functional dyspepsia with successful eradication therapy. Subgroup analyses were performed based on the type of antibiotic, and the duration of treatment, whether or not patients had symptoms of irritable bowel syndrome, and on race. Results Sixteen randomized controlled trials met the inclusion criteria. The summary OR for symptomatic improvement in patients in our eradication group was 1.33 (95% confidence interval [CI], 1.16-1.54; P < 0.01). In a subgroup analysis on type of antibiotic, symptomatic improvement with metronidazole-containing regimen (OR, 1.87; 95% CI, 1.26-2.77) was better than treatment with clarithromycin (OR, 1.29; 95% CI, 1.11-1.50). H. pylori eradication therapy given for 10-14 days was the more effective for symptom improvement than 7-day therapy. When the studies excluding irritable bowel syndrome cases were analyzed, there were no therapeutic effects of H. pylori eradication on symptomatic improvement. Conclusions In the clinical setting, the most effective H. pylori eradication regimen for functional dyspepsia to provide relief of symptoms is a metronidazole-based treatment regimen for at least 10 days. The explanation for this is that H. pylori-associated functional dyspepsia could be associated with dysbiosis. (J Neurogastroenterol Motil 2017;23:504-516)

Changes in the Prevalence of Childhood Asthma in Seoul from 1995 to 2008 and Its Risk Factors

Kwon, Ji-Won,Kim, Byoung-Ju,Song, Younghwa,Seo, Ju-Hee,Kim, Tae-Hee,Yu, Jinho,Kim, Hyo Bin,Lee, So Yeon,Kim, Woo-Kyung,Kim, Kyoung-Won,Ji, Hye-Mi,Kim, Kyu-Earn,Kim, Ho,Hong, Soo-Jong The Korean Academy of Asthma, Allergy and Clinical 2011 Allergy, Asthma & Immunology Research Vol.3 No.1

<P><B>Purpose</B></P><P>To investigate the prevalence of asthma and determine its risk factors in elementary school students in Seoul.</P><P><B>Methods</B></P><P>A modified International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire was used to survey 4,731 elementary school students from five areas in Seoul between April and October, 2008.</P><P><B>Results</B></P><P>In elementary school children, the lifetime and recent 12-month prevalence of wheezing were 11.7% and 5.6%, respectively. The lifetime prevalence of asthma diagnosis was 7.9%, and the recent 12-month prevalence of asthma treatment was 2.7%. Male sex (adjusted odds ratio [aOR], 1.90; 95% confidence interval [CI], 1.36-2.66), history of atopic dermatitis (AD) (aOR, 2.76; 95% CI, 1.98-3.84), history of allergic rhinitis (AR) (aOR, 3.71; 95% CI, 2.61-5.26), history of bronchiolitis before 2 years of age (aOR, 2.06; 95% CI, 1.39-3.07), use of antibiotics during infancy for >3 days (aOR, 1.88; 95% CI, 1.35-2.62), parental history of asthma (aOR, 2.83; 95% CI, 1.52-5.27), exposure to household molds during infancy (aOR, 1.84; 95% CI, 1.18-2.89), and the development or aggravation of asthma symptoms within 6 months after moving to a new house (aOR, 11.76; 95% CI, 5.35-25.86) were the independent risk factors for wheezing within 12 months.</P><P><B>Conclusions</B></P><P>The prevalence of wheezing and asthma in elementary school students in 2008 was similar to that in the past decade. Male sex, history of AD, history of AR, history of bronchiolitis before 2 years of age, parental asthma, use of antibiotics during infancy, exposure to molds in the house during infancy, and development or aggravation of asthma symptoms within 6 months after moving to a new house, could be risk factors for wheezing within 12 months.</P>

Rewiring of PDZ Domain-Ligand Interaction Network Contributed to Eukaryotic Evolution

Kim, Jinho,Kim, Inhae,Yang, Jae-Seong,Shin, Young-Eun,Hwang, Jihye,Park, Solip,Choi, Yoon Sup,Kim, Sanguk Public Library of Science 2012 PLoS genetics Vol.8 No.2

<▼1><P>PDZ domain-mediated interactions have greatly expanded during metazoan evolution, becoming important for controlling signal flow via the assembly of multiple signaling components. The evolutionary history of PDZ domain-mediated interactions has never been explored at the molecular level. It is of great interest to understand how PDZ domain-ligand interactions emerged and how they become rewired during evolution. Here, we constructed the first human PDZ domain-ligand interaction network (PDZNet) together with binding motif sequences and interaction strengths of ligands. PDZNet includes 1,213 interactions between 97 human PDZ proteins and 591 ligands that connect most PDZ protein-mediated interactions (98%) in a large single network via shared ligands. We examined the rewiring of PDZ domain-ligand interactions throughout eukaryotic evolution by tracing changes in the C-terminal binding motif sequences of the PDZ ligands. We found that interaction rewiring by sequence mutation frequently occurred throughout evolution, largely contributing to the growth of PDZNet. The rewiring of PDZ domain-ligand interactions provided an effective means of functional innovations in nervous system development. Our findings provide empirical evidence for a network evolution model that highlights the rewiring of interactions as a mechanism for the development of new protein functions. PDZNet will be a valuable resource to further characterize the organization of the PDZ domain-mediated signaling proteome.</P></▼1><▼2><P><B>Author Summary</B></P><P>Rewiring of interactions is a powerful tool for the evolution of organism complexity. Rewiring among preexisting proteins provides a simple mechanism for the development of new signaling circuits by redirecting information flows without a gain or loss of genes. Particularly, interactions mediated by short linear motifs can be easily changed by mutations during evolution, resulting in a rewiring of interactions. However, how interaction rewiring of linear motif interactions facilitates the emergence of new protein function during evolution is poorly understood. Here, we systematically investigated the rewiring of interactions mediated by PDZ domains, which are one of the most commonly found peptide recognition modules. We found that PDZ domain-ligand interactions are frequently rewired by C-terminal sequence mutations in PDZ ligands during evolution. Especially, rewiring of PDZ domain-ligand interactions was involved in neuronal function development, occurring concurrently with the emergence of vertebrates and suggesting that reorganization of signaling pathways by rewiring PDZ domain-ligand interactions significantly contributed to the evolution of nervous systems in vertebrates. Our findings highlight the rewiring of interactions as an effective means for functional innovation, providing new insight into eukaryotic evolution, which has not been fully explained by only the expansion of protein families.</P></▼2>

Clinical Manifestations and Genotype of Primary Ciliary Dyskinesia Diagnosed in Korea: Multicenter Study

Kim Minji,Lee Mi-Hee,Hong Soo-Jong,Yu Jinho,Cho Joongbum,서동인,Kim Hyung Young,Kim Hye-Young,Jung Sungsu,Lee Eun,Lee Sooyoung,Jeong Kyunguk,Shim Jung Yeon,Kim Jeong Hee,Chung Hai Lee,Jang Yoon Young,Kwo 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.6

Purpose: Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder that leads to secondary ciliary dysfunction. PCD is a rare disease, and data on it are limited in Korea. This study systematically evaluated the clinical symptoms, diagnostic characteristics, and treatment modalities of pediatric PCD in Korea. Methods: This Korean nationwide, multicenter study, conducted between January 2000 and August 2022, reviewed the medical records of pediatric patients diagnosed with PCD. Prospective studies have been added to determine whether additional genetic testing is warranted in some patients. Results: Overall, 41 patients were diagnosed with PCD in 15 medical institutions. The mean age at diagnosis was 11.8 ± 5.4 years (range: 0.5 months-18.9 years). Most patients (40/41) were born full term, 15 (36.6%) had neonatal respiratory symptoms, and 12 (29.3%) had a history of admission to the neonatal intensive care unit. The most common complaint (58.5%) was chronic nasal symptoms. Thirty-three patients were diagnosed with transmission electron microscopy (TEM) and 12 patients by genetic studies. TEM mostly identified outer dynein arm defects (alone or combined with inner dynein arm defects, n = 17). The genes with the highest mutation rates were DNAH5 (3 cases) and DNAAF1 (3 cases). Rare genotypes (RPGR, HYDIN, NME5) were found as well. Chest computed tomography revealed bronchiectasis in 33 out of 41 patients. Among them, 15 patients had a PrImary CiliAry DyskinesiA Rule score of over 5 points. Conclusions: To our knowledge, this is the first multicenter study to report the clinical characteristics, diagnostic methods, and genotypes of PCD in Korea. These results can be used as basic data for further PCD research.

2,4-Dinitrofluorobenzene modifies cellular proteins and induces macrophage inflammatory protein-2 gene expression via reactive oxygen species production in RAW 264.7 cells.

Kim, Dongbum,Kim, Young-Jin,Seo, Jae-Nam,Kim, Jinho,Lee, Younghee,Park, Cheung-Seog,Kim, Dae-Won,Kim, Doo-Sik,Kwon, Hyung-Joo Marcel Dekker 2009 Immunological investigations Vol.38 No.2

<P>The skin sensitizer 2,4-dinitrofluorobenzene (DNFB) provokes delayed hypersensitivity responses as a result of topical application to the skin. Here, we demonstrate that DNFB modifies proteins in RAW 264.7 cells and skin tissues in NC/Nga mice; we also show the functional involvement of DNFB-induced modification of cellular proteins in the DNFB-induced macrophage inflammatory protein (MIP)-2 gene expression in RAW 264.7 cells. In addition, we demonstrate that DNFB strongly induces reactive oxygen species (ROS) production. Our RT-PCR analysis and reporter gene assays reveal that the DNFB-induced intracellular ROS production is necessary for MIP-2 gene expression by DNFB. We observed that the vitamin C and chemical oxidant scavenger N-acetyl-cysteine have an inhibitory effect on the generation of ROS, the activation of MAP kinase pathways, and the MIP-2 gene expression in DNFB-treated RAW 264.7 cells. These results provide insight into the mechanisms involved in DNFB-induced contact hypersensitivity.</P>

Lung Disease Diagnostic Model Through IgG Sensitization to Microbial Extracellular Vesicles

Jinho Yang,Goohyeon Hong,Youn Seup Kim,Hochan Seo,Sungwon Kim,Andrea McDowell,Won Hee Lee,You-Sun Kim,Yeon-Mok Oh,조유숙,Young Woo Choi,You-Young Kim,지영구,김윤근 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.4

Purpose: Recently, there has been a rise in the interest to understand the composition of indoor dust due to its association with lung diseases such as asthma, chronic obstructive pulmonary disease (COPD) and lung cancer. Furthermore, it has been found that bacterial extracellular vesicles (EVs) within indoor dust particles can induce pulmonary inflammation, suggesting that these might play a role in lung disease. Methods: We performed microbiome analysis of indoor dust EVs isolated from mattresses in apartments and hospitals. We developed diagnostic models based on the bacterial EVs antibodies detected in serum samples via enzyme-linked immunosorbent assay (ELISA) in this analysis. Results: Proteobacteria was the most abundant bacterial EV taxa observed at the phylum level while Pseudomonas, Enterobacteriaceae (f) and Acinetobacter were the most prominent organisms at the genus level, followed by Staphylococcus. Based on the microbiome analysis, serum anti-bacterial EV immunoglobulin G (IgG), IgG1 and IgG4 were analyzed using ELISA with EV antibodies that targeted Staphylococcus aureus, Acinetobacter baumannii, Enterobacter cloacae and Pseudomonas aeruginosa. The levels of anti-bacterial EV antibodies were found to be significantly higher in patients with asthma, COPD and lung cancer compared to the healthy control group. We then developed a diagnostic model through logistic regression of antibodies that showed significant differences between groups with smoking history as a covariate. Four different variable selection methods were compared to construct an optimal diagnostic model with area under the curves ranging from 0.72 to 0.81. Conclusions: The results of this study suggest that ELISA-based analysis of anti-bacterial EV antibodies titers can be used as a diagnostic tool for lung disease. The present findings provide insights into the pathogenesis of lung disease as well as a foundation for developing a novel diagnostic methodology that synergizes microbial EV metagenomics and immune assays.

Diagnosis of Lymphoid Malignancy by PCR for Analysis of Antigen Receptor Rearrangement after Blood Transfusion in a Dog with Acute Lymphocytic Leukemia

Kim, Suhee,Kim, Hyunwoo,Lee, Soo-Hyeon,Cho, Ilhan,Kang, Seongwoo,Bae, Junwoo,Kim, Woosun,Ahn, Soomin,Choi, Jihye,Kim, Sang-Ki,Do, Yoonjung,Yoo, Jae Gyu,Park, Jinho,Yu, DoHyeon 한국조명·전기설비학회 2017 한국조명·전기설비학회 학술대회논문집 Vol. No.

<P>Acute lymphocytic leukemia (ALL) is uncommon lymphoid malignancy in dogs, and its diagnosis is challenging. A 14-year-old spayed female mixed breed dog was transferred to a veterinary medical teaching hospital for an immediate blood transfusion. The dog showed lethargy, pale mucous membranes, and a weak femoral pulse. Complete blood count revealed non-regenerative anemia and severe leukopenia with thrombocytopenia. ALL was tentatively diagnosed based on the predominance of immature lymphoblasts on blood film examination. For confirmation of lymphoid malignancy, PCR for antigen receptor rearrangement (PARR) on a peripheral blood sample and flow cytometry analysis were performed after blood transfusion. Flow cytometry analysis revealed that lymphocyte subsets were of normal composition, but PARR detected a T-cell malignancy. The dog was diagnosed with ALL and survived 1 wk after diagnosis. In conclusion, after blood transfusion, flow cytometry was not a reliable diagnostic method for an ALL dog, whereas PARR could detect lymphoid malignancy. Our results suggest that PARR should be the first-line diagnostic tool to detect canine lymphoid malignancy after a blood transfusion.</P>