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안병만,김용길,차병묵,피윤섭,윤재홍,김진관 國立 昌原大學校 産業技術硏究所 1994 産技硏論文集 Vol.8 No.-
STD61종 열간금형강의 수명향상을 위하여 ion nitriding처리를 실시하였다. 이온질화전의 모재의 열처리시에는 1030˚C에서 quenching한 후 530˚C에서 2시간 tempering했을 때 Hv=600의 높은 경도값을 나타내었다. 580˚C, 600˚C에서 2시간 tempering했을 때는 각각 Hv=500, Hv=400으로 경도값이 오히려 감소했다. 이온질화 처리시에는 처리시간이 길고 N₂와 Ar첨가유량이 많을 수록 깊은 화합물층과 확산층을 얻었다. 그리고 각 처리조건에서 안정된 Hv=1150의 질화물층을 얻을 수 있다. Ion nitriding treatment has been practiced to improve the life of hot die steel of STD61. Before nitriding the parent STD61 tempered at 530˚C, 2hrs after quenching at 1030˚C, It showed the high hardness distribution of Hv=600. The hardness distribution has decreased by Hv=400, Hv=500 when tempered for 2hrs at 580˚C, 600˚C. In the ion nitriding, the more treat-time and the more N₂and Ar flux, the deeper the compounded layer and the diffused layer. The stable hardness, Hv=1150, of nitride layer was obtained at various treatment condition.
김준섭,권오구,박진석,오종수,박정현,박제식,안세한,이용환,서정호,신태섭,최영배,김덕수,이관,박수경 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.1
본 연구는 경주지역 1개 고등학교 3학년 여고생을 대상으로 초경 시작 전인 초등학교 4학년부터 고등학교 2학년까지의 키, 몸무게, 가슴둘레 수치와 초경 연령을 파악하여 여성의 초경이 빠를수록 체성장 속도의 변화가 있을 것이라는 가설을 기초로 두 요인간의 관련성을 파악하고자 시행되었다. 경주시 1개 여자고등학교 3학년생 총 317명에 대해 ㅊ경 나이, 초경시 심리 상태, 초경이 시작되었을 때의 상담 여부와 상담하였던 사람, 부모 신장과 초경전후의 식사 규칙성, 다이어트 경험, 수면시간, 스트레스, 음주/흡연 여부, 질병력 등에 대한 설문 조사를 시행하였고, 초등학교 4학년부터 고등학교 2학년까지의 키, 몸무게, 가슴둘레에 대한 정보는 건강기록부에서 확보하였다. 상기 대상자 중 건강기록부가 분실된 39명, 초경나이에 대해 응답하지 않았던 29명 및 결석 등으로 조사를 하지 못하였던 42명을 제외한 조사 대상자는 206명으로 선정하였다. 대상자들의 초경연령 평균값(Mean±SD)은 13.0±1.11이었고, 초등학교 4학년부터 고등학교 2학년까지의 초경 이후 신장 성장률은 감소하였으며, 초경연령이 빠른 군이 초등학교 때는 신장에 있어서 우위를 차지하나 고등학교에 오면서 신장은 비슷한 수준이 되었다. 초경후 1-2년 사이에 체중과 흉위의 증가율은 감소하였고, 초경연령이 빠른 군이 초등학교와 고등학교 사이에 체중과 흉위에서 모두 우위를 차지하였다. 본 연구의 결과는, 흔히 이차 성징의 시작 시점 혹은 사춘기의 시작 시점으로 보고있는 초경 연령이 청소년기의 성장과 밀접한 관련성이 있고, 특히 체중과 흉위의 성장 속도와 밀접한 관련성이 있음을 시사한다. To understand the onset of menarche in relation to changes in physical growth that take place during female adolescence, especially the changes in height and weight velocity. The 207 senior in one women high school of Kyongju were investigated in April 3 to 8, 2001. We collected the information, relation in the age at menarche, height & weight of parents, regularity of eating, and obesity diet, duration of sleeping, sickness & stress near age at menarche, by self-recording. The status of height, weight & chest circumference between the 4th grade of primary school and the junior of high school was collected by individual Health Record. The results were followed; The average age at menarche was 12.95±1.11 years old and the most frequent age of menarche is 13 years. After menarche, the velocity of height, weight and chest circumference were decreased. The most rapid group of menarche hold dominant position in height during elementary school. As time passed, they dont hold dominant position in height. The most rapid group of menarche get an advantage in the weight and the girth of chest after elementary school. The height of subjects is directly proportional to the height of their parents. These results suggest that the starting age at menestuation can be closely associated with physical growth and development.
Ahn, Jin Seop,Ryu, Hyun-Sung,Jung, Sang-Eun,Shin, Beom-Jin,Won, Jong-Hyun,Um, Tea Gun,Oh, Huijo,Kim, Seo-Hee,Ryu, Buom-Yong The Korean Society of Animal Reproduction and Biot 2020 한국동물생명공학회지 Vol.35 No.4
Spermatogonial stem cells are self-renewal and differentiate into sperm in post-pubertal mammals. There exists a balance between the self-renewal and differentiation in the testes. Spermatogonial stem cells make up only 0.03% of testicular cells in adult mice. These cells maintain sperm production by differentiating after puberty. Therefore, analyzing the expression of genes associated with spermatogenesis is critical for understanding differentiation. The present study aimed to establish the postnatal period of cells in relation to spermatogenesis. To study the expression of differentiated and undifferentiated marker genes in enriched spermatogonial stem cells, in vitro culture was performed and cells from pup (6-8-day-old) and adult (4-months-old) testicular tissues were isolated. As a result, undifferentiated genes, Pax7, Plzf, GFRa1, Etv5 and Bcl6b, were highly increased in cultured spermaotogonial stem cells compared with pup and adult testicular cells. On the other hands, differentiated gene, c-kit was highly increased in adult testicular cells, Also Stra8 gene was highly increased in pup and adult testicular cells. This study provides a better understanding of spermatogenesis-associated gene expression during postnatal periods.
Jin Seop Ahn,Hyun-Sung Ryu,Sang-Eun Jung,Beom-Jin Shin,Jong-Hyun Won,Tea Gun Um,Huijo Oh,Seo-Hee Kim,Buom-Yong Ryu 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.4
Spermatogonial stem cells are self-renewal and differentiate into sperm in post-pubertal mammals. There exists a balance between the self-renewal and differentiation in the testes. Spermatogonial stem cells make up only 0.03% of testicular cells in adult mice. These cells maintain sperm production by differentiating after puberty. Therefore, analyzing the expression of genes associated with spermatogenesis is critical for understanding differentiation. The present study aimed to establish the postnatal period of cells in relation to spermatogenesis. To study the expression of differentiated and undifferentiated marker genes in enriched spermatogonial stem cells, in vitro culture was performed and cells from pup (6–8-day-old) and adult (4-months-old) testicular tissues were isolated. As a result, undifferentiated genes, Pax7, Plzf, GFRa1, Etv5 and Bcl6b , were highly increased in cultured spermaotogonial stem cells compared with pup and adult testicular cells. On the other hands, differentiated gene, c-kit was highly increased in adult testicular cells, Also Stra8 gene was highly increased in pup and adult testicular cells. This study provides a better understanding of spermatogenesis-associated gene expression during postnatal periods.
Expression of the C1orf31 Gene in Human Embryonic Stem Cells and Cancer Cells
Ahn, Jin-Seop,Moon, Sung-Hwan,Yoo, Jung-Ki,Jung, Hyun-Min,Chung, Hyung-Min,Kim, Jin-Kyeoung The Korean Society of Animal Reproduction 2008 Reproductive & developmental biology Vol.32 No.4
Human embryonic stem (ES) cells retain the capacity for self-renewal, are pluripotent and differentiate into the three embryonic germ layer cells. The regulatory transcription factors Oct4, Nanog and Sox2 play an important role in maintaining the pluripotency of human ES cells. The aim of this research was to identify unknown genes upregulated in human ES cells along with Oct4, Nanog, and Sox2. This study characterizes an unknown gene, named chromosome 1 open reading frame 31 (C1orf31) mapping to chromosome 1q42.2. The product of C1orf31 is the hypothetical protein LOC388753 having a cytochrome c oxidase subunit VIb (COX6b) motif. In order to compare expression levels of C1orf31 in human ES cells, human embryoid body cells, vascular angiogenic progenitor cells (VAPCs), cord-blood endothelial progenitor cells (CB-EPCs) and somatic cell lines, we performed RT-PCR analysis. Interestingly, C1orf31 was highly expressed in human ES cells, cancer cell lines and SV40-immortalized cells. It has a similar expression pattern to the Oct4 gene in human ES cells and cancer cells. Also, the expression level of C1orf31 was shown to be upregulated in the S phase and early G2 phase of synchronized HeLa cells, leading us to purpose that it may be involved in the S/G2 transition process. For these reasons, we assume that C1orf31 may play a role in on differentiation of human ES cells and carcinogenesis.
Expression of the C1orf31 Gene in Human Embryonic Stem Cells and Cancer Cells
Jin seop Ahn,Jung Ki Yoo,Hyun Min Jung,Jin Kyeoung Kim,Sung-Hwan Moon,Hyung-Min Chung 한국동물생명공학회(구 한국동물번식학회) 2008 Reproductive & developmental biology Vol.32 No.4
Human embryonic stem (ES) cells retain the capacity for self‐renewal, are pluripotent and differentiate into the three embryonic germ layer cells. The regulatory transcription factors Oct4, Nanog and Sox2 play an important role in maintaining the pluripotency of human ES cells. The aim of this research was to identify unknown genes upregulated in human ES cells along with Oct4, Nanog, and Sox2. This study characterizes an unknown gene, named chromosome 1 open reading frame 31 (C1orf31) mapping to chromosome 1q42.2. The product of C1orf31 is the hypothetical protein LOC388753 having a cytochrome c oxidase subunit VIb (COX6b) motif. In order to compare expression levels of C1orf‐ 31 in human ES cells, human embryoid body cells, vascular angiogenic progenitor cells (VAPCs), cord‐blood endothelial progenitor cells (CB‐EPCs) and somatic cell lines, we performed RT‐PCR analysis. Interestingly, C1orf31 was highly expressed in human ES cells, cancer cell lines and SV40‐immortalized cells. It has a similar expression pattern to the Oct4 gene in human ES cells and cancer cells. Also, the expression level of C1orf31 was shown to be upregulated in the S phase and early G2 phase of synchronized HeLa cells, leading us to purpose that it may be involved in the S/G2 transition process. For these reasons, we assume that C1orf31 may play a role in on differentiation of human ES cells and carcinogenesis.
제대 혈청 IgE 치 ( Ultra RIA ) 에 대한 고찰
강진섭(Jin Seop Kang),고재욱(Jae Wook Ko),홍영진(Young Jin Hong),안돈희(Don Hee Ahn),이명익(Myung Ik Lee) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 1995 소아알레르기 및 호흡기학회지 Vol.5 No.1
N/A The objectives of this report is to evaluate and determine the normal level of cord serum IgE which is regarded as one of successful parameters , predicting the development of atopic disease in neonate. Forty mothers who were admitted to NMC for the purpose of fullterm delivery and their newborns were investigated. We had titrated cord serum IgE levels by new method , IgE Ultra RIA, and also checked maternal serum IgE by IgE RIA method. Additionally, allergic and smoking histories of mothers and informations of neonate were taken for comparison of cord serum IgE levels . The following results were obtained. 1. The median value of cord serum IgE measured by Ultra RIA method was 0.22(range : 0.11-2.19) IU/ml and that of maternal serum IgE by RIA method was 59.5(range 6-448) IU/ml. 2. There was no significant correlation between log10 value of maternal and cord serum IgE. 3. According to the sex and birth weight, there was no significant differences of cord serum IgE levels. 4. According to birth order , cord serum IgE levels of first babies showed significantly higher than those of others. 5. According to allergic family history and maternal smoking history , there was no significant difference of cord serum IgE levels .
발아 중인 옥수수에서 리보조옴 단백질의 인산화반응에 미치는 GA3 의 효과
안경섭(Kyung Seop Ahn),음진성(Jin Seong Eum),심웅섭(Woong Seop Sim) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.1
In order to study the effect of GA_3 on the phosphorylation of ribosomal proteins during germination in Zea mays, ribosomal proteins were labelled with ^32P, extracted, electrophoresed and autoradiographed. There are five phosphorylated ribosomal proteins. One of these is in 40S subunit and has molecular weight of 33,000 daltons. Others are in 60S subunit and have molecular weights of 37,000, 16,000, 15,200 and 13,500, respectively. Phosphorylation of ribosomal proteins was increased maximum 47.7% in shoots of Zea mays treated with GA_3.