Effect of the Molar Ratio of Li/Ti and Thermal Treatment on the Electrochemical Performance of Li4Ti5O12–rutile TiO2 Nanocomposite as Anode Materials

Zhen Yang,Xi-Ping Li,Jian Mao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.8

Li4Ti5O12–rutile TiO2 (LTO–RTO) dual-phase nanocomposite anode materials show excellent electrochemical performance. However, the effects of molar ratio of Li/Ti and thermal treatment on electrochemical properties of the LTO–RTO composite have been rarely reported. In this work, LTO–RTO nanocomposites were prepared by sol-hydrothermal method with different Li/ Ti molar ratios in raw materials and following calcinations at 600℃, 650℃ and 700℃ for the different holding time. The results indicate that with the decrease of Li/Ti molar ratio, the discharge capacity of the LTO–RTO nanocomposite increases at first and then decreases, and the optimal Li/Ti molar ratio is 4:4.77, which was obtained with calcination at 600℃ for 10 h. The effects of calcination temperature and holding time were further investigated. The result demonstrates that the thermal treatment has an obvious influence on the electrochemical performance due to the morphology change in the nanocomposite. The LTO–RTO nanocomposite calcinated at 650℃ for 2 h with a Li/Ti molar ratio of 4:4.77 in raw materials delivers excellent rate capability: the initial discharge capacity is 175.9, 176.3, 170.4, 167.5, 163.3 and 155.6 mA h g-1 at the rate of 0.5, 1, 3, 5, 10 and 20℃ (1 C = 175 mA h g-1), respectively.

DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer

Jian‑Cheng Zhai,Ruo‑Bing Han,Sheng‑Nan Wang,Qiang‑Hui Wang,Yan‑Ling Xia,Wei‑Shi Liu,Ya‑Jie Yin,He‑Ping Li 한국유전학회 2019 Genes & Genomics Vol.41 No.9

Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.

Vector Passive Harmonic Mode-locking Fiber Laser Based on Topological Insulator Bi2Se3 Interacting with Fiber Taper

Jian Ping Li 한국광학회 2016 Current Optics and Photonics Vol.20 No.1

I propose a vector passive harmonic mode-locked fiber laser based on topological insulator Bi2Se3interacting with a fiber taper with a diameter of 7 μm. The particles of topological insulator are depositeduniformly onto the fiber taper with light pressure effect. By incorporating the fabricated saturable absorberinto an Er-doped fiber laser cavity, stable mode-locked fiber is obtained. Due to the intense evanescentfield of the fiber taper, strong confinement of light enhances the nonlinearity of the laser cavity, and passiveharmonic mode-locking is performed. I observe a maximum harmonic mode-locking of 356th, correspondingto a frequency of 3.57 GHz. The pulse duration is 824 fs, and the full width at half maximum of thespectrum is about 8.2 nm. The polarization dependent loss of the saturable absorber is ~ 2.5 dB in thewavelength range of the C band. As the cavity contains no other polarization dependent device, themode-locked laser is functioning in the vector state. The harmonic order vs pump power is investigated. To the best of our knowledge, this report is the highest frequency mode-locked fiber laser based on Bi2Se3. Experimental results indicate that the topological insulator Bi2Se3 functioning with a thin fiber taper iseffective for vector harmonic mode-locking.

OPC Unified Architecture for Industrial Demand Response

Jian-ping Chou,Li-chao Chen,YingJun Zhang,Li-hu Pan 보안공학연구지원센터 2012 International Journal of Security and Its Applicat Vol.6 No.2

Diversity and Distribution of Methanogenic Archaea in an Anaerobic Baffled Reactor (ABR) Treating Sugar Refinery Wastewater

( Jian Zheng Li ),( Li Guo Zhang ),( Qiao Ying Ban ),( Ajay Kumar Jha ),( Yi Ping Xu ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.2

The diversity and distribution of methanogenic archaea in a four-compartment anaerobic baffled reactor (ABR) treating sugar refinery wastewater were investigated by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). At an organic loading rate of 5.33 kg COD/m3·day, the ABR could perform steadily with the mean chemical oxygen demand (COD) removal of 94.8% and the specific CH4 yield of 0.21 l/g CODremoved. The CH4 content in the biogas was increased along the compartments, whereas the percentage of H2 was decreased, indicating the distribution characteristics of the methanogens occurred longitudinally down the ABR. A high phylogenetic and ecological diversity of methanogens was found in the ABR, and all the detected methanogens were classified into six groups, including Methanomicrobiales, Methanosarcinales, Methanobacteriales, Crenarchaeota, Arc I, and Unidentified. Among the methanogenic population, the acid-tolerant hydrogenotrophic methanogens including Methanoregula and Methanosphaerula dominated the first two compartments. In the last two compartments, the dominant methanogenic population was Methanosaeta, which was the major acetate oxidizer under methanogenic conditions and could promote the formation of granular sludge. The distribution of the hydrogenotrophic (acid-tolerant) and acetotrophic methanogens in sequence along the compartments allowed the ABR to perform more efficiently and steadily.

Study on mechanism of macro failure and micro fracture of local nearly horizontal stratum in super-large section and deep buried tunnel

Li, Shu-cai,Wang, Jian-hua,Chen, Wei-zhong,Li, Li-ping,Zhang, Qian-qing,He, Peng Techno-Press 2016 Geomechanics & engineering Vol.11 No.2

The stability of surrounding rock will be poor when the tunnel is excavated through nearly horizontal stratum. In this paper, the instability mechanism of local nearly horizontal stratum in super-large section and deep buried tunnel is revealed by the analysis of the macro failure and micro fracture. A structural model is proposed to explain the mechanics of surrounding rock collapse under the action of stress redistribution and shed light on the macroscopic analytical approach of the stability of surrounding rock. Then, some highly effective formulas applied in the tunnel engineering are developed according to the theory of mixed-mode micro fracture. And well-documented field case is made to demonstrate the effectiveness and accuracy of the proposed analytical methods of mixed-mode fracture. Meanwhile, in order to make the more accurate judgment about yield failure of rock mass, a series of comprehensive failure criteria are formed. In addition, the relationship between the nonlinear failure criterion and $K_I$ and $K_{II}$ of micro fracture is established to make the surrounding rock failure criterion more comprehensive and accurate. Further, the influence of the parameters related to the tension-shear mixed-mode fracture and compression-shear mixed-mode fracture on the propagation of rock crack is analyzed. Results show that ${\sigma}_3$ changes linearly with the change of ${\sigma}_1$. And the change rate is related to ${\beta}$, angle between the cracks and ${\sigma}_1$. The proposed simple analytical approach is economical and efficient, and suitable for the analysis of local nearly horizontal stratum in super-large section and deep buried tunnel.

Expression, Purification, and Biological Characterization of The Amino-Terminal Fragment of Urokinase in Pichia pastoris

( Jian Ping Li ),( Yu Li Lin ),( Hong Qin Zhuang ),( Zi Chun Hua ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.9

Urokinase (uPA) and its receptor (uPAR) play an important role in tumor growth and metastasis. Targeting the excessive activation of this system as well as the proliferation of the tumor vascular endothelial cell would be expected to prevent tumor neovasculature and halt the tumor development. In this regard, the amino-terminal fragment (ATF) of urokinase has been confirmed as effective to inhibit the proliferation, migration, and invasiveness of cancer cells via interrupting the interaction of uPA and uPAR. Previous studies indicated that ATF expressed in Escherichia coli was mainly contained in inclusion bodies and also lacked posttranslational modifications. In this study, the biologically active and soluble ATF was cloned and expressed in Pichia pastoris. The recombinant protein was purified to be homogenous and confirmed to be biologically active. The yield of the active ATF was about 30 mg/l of the P. pastoris culture medium. The recombinant ATF (rATF) could efficiently inhibit angiogenesis, endothelial cell migration, and tumor cell invasion in vitro. Furthermore, it could inhibit in vivo xenograft tumor growth and prolong the survival of tumor-bearing mice significantly by competing with uPA for binding to cell surfaces. Therefore, P. pastoris is a highly efficient and cost-effective expression system for large-scale production of biologically active rATFs for potential therapeutic application.

Structural instantaneous frequency extraction based on improved multi-synchrosqueezing generalized S-transform

Ping-Ping Yuan,Xue-Li Cheng,Hang-Hang Wang,Jian Zhang,Zhong-Xiang Shen,Wei-Xin Ren 국제구조공학회 2021 Smart Structures and Systems, An International Jou Vol.28 No.5

A new method is proposed to improve the accuracy of structural instantaneous frequency (IF) extraction. The proposed method combines a new form of improved generalized S-transform (IGST) and a multi-synchrosqueezing operation. The parameters selection of the window function in IGST is derived through the concentration measure (CM) principle. Then, the multi-synchrosqueezing algorithm is employed to improve energy aggregation of time-frequency analysis (TFA). To verify the effectiveness and accuracy of the proposed improved multi-synchrosqueezing generalized S-transform (IMSSGST), a frequency-modulated multi-component signal is investigated. For structural IF extraction, a two-story shear frame and a threestory steel frame structure are introduced. Furthermore, the IF identification of a seven-story RC shear wall structure is conducted to verified the practicability in actual engineering. Numerical simulation and experimental results show that the proposed method can effectively improve the energy aggregation of TFA and effectively improve the accuracy of IF identification.

A Human Case of Lumbosacral Canal Sparganosis in China

Jian-Feng Fan,Sheng Huang,Jing Li,Ren-Jun Peng,He Huang,Xi-Ping Ding,Li-Ping Jiang,Jian Xi 대한기생충학열대의학회 2021 The Korean Journal of Parasitology Vol.59 No.6

MiRNA-15a Mediates Cell Cycle Arrest and Potentiates Apoptosis in Breast Cancer Cells by Targeting Synuclein-γ

Li, Ping,Xie, Xiao-Bing,Chen, Qian,Pang, Guo-Lian,Luo, Wan,Tu, Jian-Cheng,Zheng, Fang,Liu, Song-Mei,Han, Lu,Zhang, Jian-Kun,Luo, Xian-Yong,Zhou, Xin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: Recent studies have indicated that microRNA-15a (miR-15a) is dysregulated in breast cancer (BC). We aimed to evaluate the expression of miR-15a in BC tissues and corresponding para-carcinoma tissues. We also focused on effects of miR-15a on cellular behavior of MDA-MB-231 and expression of its target gene synuclein-${\gamma}$ (SNCG). Materials and Methods: The expression levels of miR-15a were analysed in BC formalin fixed paraffin embedded (FFPE) tissues by microarray and quantitative real-time PCR. CCK-8 assays, cell cycle and apoptosis assays were used to explore the potential functions of miR-15a in MDA-MB-231 human BC cells. A luciferase reporter assay confirmed direct targets. Results: Downregulation of miR-15a was detected in most primary BCs. Ectopic expression of miR-15a promoted proliferation and suppressed apoptosis in vivo. Further studies indicated that miR-15a may directly interact with the 3'-untranslated region (3'-UTR) of SNCG mRNA, downregulating its mRNA and protein expression levels. SNCG expression was negatively correlated with miR-15a expression. Conclusions: MiR-15a has a critical role in mediating cell cycle arrest and promoting cell apoptosis of BC, probably by directly targeting SNCG. Thus, it may be involved in development and progression of BC.