SH-SY5Y Human Neuroblastoma Cell에서 에스트로겐의 신경세포 보호효과에 대한 기전

염지현,김희,홍현석,방오영,허균,묵인희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.1

Apoptosis is one major mechanism underlying neuronal cell death in degenerative diseases in the central nervous system. Previous studies have shown that estrogen has neuroprotective effects in several neuronal cell death model systems. In the present study, we established a staurosporine-induced apoptotic neuronal cell death system with human SH-SY5Y cell line. 17b-estradiol(E2) blocked staurosporine-induced apoptosis of SH-SY5Y cells as revealed by MTT as well as LDH assay. The cells showed typical DNA fragmentation at 4 h and 8 h following staurosporine treatment, which was attenuated by E2 pretreatment. Staurosporine-induced chromatin condensation was also reduced by E2 treatment. Because apoptotic stimuli are known to induce caspase-3 activation and PARP cleavage, we examined whether E2 blocks these processes. E2 markedly attenuated staurosporine-induced casapase-3 activation and PARP cleavage. This study shows that E2 blocks staurosporine-induced apoptosis in SH-SY5Y human neuroblastoma cells, suggesting that E2 is a neuroprotective agent that may be used for the treatment of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.

제주 지역의 B형 간염 바이러스 유전자형

조지현,박도심,김태현,염주진,김학철,문주현,양재식 대한간학회 2004 Clinical and Molecular Hepatology(대한간학회지) Vol.10 No.1

목적: HBV 감염이 무증상의 자연 회복부터 간경변 혹은 간세포성 간암까지 다양하게 나타나는 요인들로 숙주의 요인과 바이러스의 요인으로 구분되어 제시되는데, 바이러스 요인으로는 감염된 바이러스이 유형이나 염기 변이들이 논의된다. HBV 유형을 구분하는 데는 혈청형과 유전자형이 이용되는데 근자에는 주로 유전자형이 이용된다. 이러한 HBV의 분포는 지역적으로 다르나 지역에 따라서 다른 유형들이 혼재되어 있다. 동남아시아는 B형이지만 극동아시아는 C형으로 알려져 있으며, 대만과 오키나와에서는 B와 C형이 혼재되어 나타난다. 이러한 결과로 미루어 보아 우리 나라의 남부지역에서는 B과 C형이 혼재되어 있을 가능성이 있다. 이에 본 연구는 제주 지역에서 HBV의 유전자형을 조사하고자 하였다. 대상과 방법: 원적지가 제주도이면사, HBsAg가 양성이었던 107명(평균 연령: 46.5세)을 대상으로 하였다. 이들의 혈청으로부터 HBsAg/antiHBe를 분석하고, DNA를 추출하여 7개의 유전자형에 따라 특이성을 갖는 시발체(genotype specific primer)들을 이용하여 PCR로 증폭한 후 이를 분석하였다. 주된 유전자형의 일부와 다른 유형에서 증폭에 이용되었던 시발체를 다르게 이용하여 hemi nested PCR로 증폭한 후 염기를 분석하여 계통발생학적 분석법을 이용하였다. 결과: 1. PCR에 의한 HBV DNA 양성은 107명 중 59명(55.1%)이 양성이었다. HBeAg/anti HBe의 발현 양태에 따라 음성/음성에서 2명(66.7%), 음성/양성에서 30명(41.1%), 양성/음성에서 24명(85.7%), 양성/양성에서는 3명(100%)에서 증폭되었다. 2. Multiplex PCR에서는 58명의 유전자형이 C형, 1명에서는 B형과 C형이 동반 출현하였다. 3. Genotype specific primer를 이용하여 각각 따로 PCR을 시행한 경우에 A형에 의하여 증폭된 예가 1예, B형에 의하여 증폭된 예가 1예, C형에 의하여 증폭된 예가 59예로 A형과 B형 모두가 C형에서 증폭된 예였다. 4. 한 쌍의 시발체에 의하여 증폭되었던 A형과 B형 및 C형의 일부에서 염기를 분석한 결과 모두 C 유전자형이었다. 결론: HBsAg이 양성인 제주 지역 주민의 HBV의 유전자형은 C형일 것으로 생각된다. Background/Aims: Hepatitis B virus (HBV) was classified into 8 genotypes by a sequence divergence in the entire genome designated from A o H. HBV genotypes have distinct geographic distributions. Recently, HBV genotypes have been partially found as influencing the clinical manifestation of chronic liver disease in hosts. In Korea, the distribution of HBV genotypes remains unclear. The aim of this study was to evaluate the prevalence of the HBV genotype on Jeju Island. Methods: Hepatitis B virus genotypes were evaluated among 107 hepatitis B carriers residing on Jeju Island. We used single PCR and multiplex-PCR assay with genotype-specific primer pairs for HBV genotypes A-F for the genotyping. Results: 1. Fifty nine samples (55%) were positive for HBV DNA. The positivity was different according to the pattern of HBeAg/anti-HBe expression, as -/-; 2/3 (66.7%), -/+; 30/73 (30%), +/-; 24/28 (85.7%) and +/+; 3/3 (100%). 2. In the single primer set of genotype-specific PCR, 59 samples (100%) were detected as genotype C and 2 (3%) were also detected as genotype A and B. 3. In multiplex-PCR, 58 samples (98%) were detected as genotype C and only one (2%) as a mixed pattern of genotype B and C. 4. When the PCR products were amplified with universal sense and genotype specific anti-sense from one genotype A, one B, and 2 C, all were included in genotype C. Conclusions: These results suggest that on Juju Island, almost all HBV genotypes are C.(Korean J Hepatol 2004;10:42-50)

Anti-Oxidative Activity of Branches of ‘Water Apple’

Hyun Sook Yeom(염현숙),Yeon Jeong Jo(조연정),Ji Hun Park(박지훈),Chan Gyu Im(임찬규),Nam Ho Lee(이남호),Ju Mi Hyun(현주미) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.2

Graft lymphoma in a kidney transplant recipient: a case report

Yeom Ji Hyun,김원,강경표,김형완,유희철,황홍필,Chung Byeoung Hoon,Jang Kyu Yun,이식 대한이식학회 2023 Korean Journal of Transplantation Vol.37 No.3

Posttransplant lymphoproliferative disorders (PTLDs) are severe complications with heterogeneous clinical pictures involving abnormal lymphoproliferation in solid organ transplants and are known to be closely associated with Epstein-Barr virus (EBV) infection. Herein, we present a case of graft lymphoma in a febrile kidney transplant recipient. A 37-year-old woman was admitted with an abrupt 39 oC fever, mild graft discomfort, and gross hematuria. She had received deceased donor kidney transplantation 8 years earlier, but developed graft failure due to a recurrence of immunoglobulin A nephropathy. Laboratory tests revealed anemia and elevated levels of inflammatory markers. Enhanced abdominopelvic computed tomography showed graft swelling with perirenal fat stranding. Thus, we administered antibiotics for a urinary tract infection and increased the doses of steroids due to suspicion of graft intolerance syndrome. However, the patient's symptoms gradually worsened. Eventually, we performed graft nephrectomy and histologically confirmed EBV-positive diffuse large B cell lymphoma. We report a case in which a PTLD was considered in the differential diagnosis of a kidney transplant recipient with symptoms similar to those of a urinary tract infection or graft intolerance syndrome.

Poster Session : Ethical Problems in Taekwondo Competitions

( Ji Hyun Yeom ),( Jae Bong Lee ) 한국체육학회 2013 In Pursuit of Evidence-Based Sport and Exercise Sc Vol.0 No.-

Functional Implications of the Conserved Action of Regulators of Ribonuclease Activity

( Ji Hyun Yeom ),( Eun Kyoung Shin ),( Ha Young Go ),( Se Hoon Sim ),( Maeng Je Seong ),( Kang Seok Lee ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.8

A Case of Encephalitis Caused by Epstein-Barr Virus Infection

Hyun-Jeong Do(도현정),Jong-Geun Baek(백종근),Jung-Sook Yeom(염정숙),Ji-Sook Park(박지숙),Eun-Sil Park(박은실),Ji-Hyun Seo(서지현),Jae-Young Lim(임재영),Chan-Hoo Park(박찬후),Hyang-Ok Woo(우향욱),Hee-Shang Youn(윤희상) 대한소아신경학회 2009 대한소아신경학회지 Vol.17 No.2

발열을 동반한 경련 이후 의식 저하를 주소로 내원한 환아에서 전형적인 전염성 단핵구증의 증상 및 비정형 림프구 혈증, 혈청학적 검사에서 양성 소견을 보여 EBV 감염에 의한 전염성 단핵구증의 합병증으로 발생한 뇌염으로 진단한 증례가 있어 보고하는 바이다. An 11-year-old girl was diagnosed with encephalitis due to Epstein-Barr virus(EBV). She was admitted to our hospital due to convulsion and decreased consciousness after several days of fever, sore throat and headache. Cerebrospinal fluid analysis showed lymphocyte-dominant pleocytosis and markedly elevated levels of protein. Magnetic resonance imaging(MRI) finding was normal, except for marked leptomeningeal enhancement. Even though she had signs and symptoms of infectious mononucleosis, EBV infection could not be considered as the underlying cause of the encephalitis until IgM for the viral capsid antigen of EBV had been detected in her blood. After treatment with acyclovir, a high dose of methylprednisolone and intravenous immunoglobulin, the neurological symptoms improved rapidly. She has not suffered from any neurological complications in the four months since being discharged. EBV infection should be considered as the main etiology in cases of childhood encephalitis, although there is no evidence of infectious mononucleosis existed.

Aptamer-conjugated gold nanoparticles platform as the intracellular delivery of antibodies for cancer therapy

Ji-Hyun Yeom,Eunkyoung Shin,Hanyong Jin,Haifeng Liu,Yongyang Luo,Youngwoo Nam,Minkyung Ryu,Wooseok Song,Heeyoun Chi,Jeongkyu Kim,Kangseok Lee,Jeehyeon Bae 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-

Antibodies are being increasingly used for therapeutic purposes due to their remarkable target specificityand affinity. However, currently available antibody therapies are restricted to target proteins in the outercell membrane or in the extracellular fluids because of the lack of technologies for effective intracellulardelivery of antibodies. Here, we report an efficient and versatile intracellular antibody delivery system. This system is based on gold nanoparticles (AuNPs) conjugated with DNA aptamers (Apt) against theFc region of IgG (AuNP-AptIgG), allowing to load any antibodies onto the AuNP-AptIgG by simple mixing. This AuNP-AptIgG-Ab platform was effective for cytosolic delivery of antibodies to clinically importantmutant proteins via scavenger receptors and caveolae-mediated endocytosis. Specifically, cancer cellsexpressing BRAFV600E, a variant of BRAF identified in numerous types of cancers, exhibited reduced cellviability by 70% when BRAFV600E antibodies were intracellularly delivered using the AuNP-AptIgG(AuNP-AptIgG-aBRAFV600E). In addition, subcutaneous injection of AuNP-AptIgG-aBRAFV600E into in vivoxenografted melanoma tumors expressing BRAFV600E resulted in both inhibition of proliferation andinduction of apoptosis, leading to tumor regression in mice. Thus, our findings indicate that the AuNPAptIgG-Ab system can serve as a promising platform for effective intracellular delivery of antibodies fortherapeutic purposes.

Levothyroxine Sodium Administration and Late Circulatory Collapse in Premature Infants with Thyroid Dysfunction

( Hyun-jeong Do ),( Jae Young Cho ),( Jung Sook Yeom ),( Ji Sook Park ),( Ji-hyun Seo ),( Jae Young Lim ),( Chan-hoo Park ),( Hyang-ok Woo ),( Hee-shang Youn ) 대한주산의학회 2019 Perinatology Vol.30 No.3

Objective: Levothyroxine sodium (LT4) is considered safe, and widely administered to premature infants with thyroid dysfunction. Late circulatory collapse (LCC) has not been defined clearly, but it has been characterized as refractory hypotension in premature infants. Recently, LCC after LT4 administration was reported in Japan. However, there is controversy on the aspect of LT4 as a risk factor of LCC. The purpose of this study was to investigate relations between LT4 administration and LCC in premature infants. Methods: We retrospectively reviewed medical records of premature infants (≤32 weeks gestation) admitted in Gyeongsang National University Hospital between 2011 and 2018. To investigate the relation between LT4 supplementation and LCC, clinical and laboratory findings were reviewed and compared between premature infants with and without LCC. Results: Among 347 premature infants, LCC occurred in 21 (6.1%) on median 19.0th day. Sixteen (76.2%) of 21 infants with LCC were receiving LT4 and LCC was developed on median 3.0 days after LT4 initiation. Gestational age ≤28 weeks, birth weight <1.5 kg, hemodynamically significant patent ductus arteriosus, culture proven sepsis, necrotizing enterocolitis, congenital hypothyroidism, use of LT4 and diuretics, values of serum thyroid stimulating hormone, free thyroxine and sodium were significantly statistically different between LCC and no LCC group. Prematurity ≤28 weeks of gestation and LT4 replacement were risk factors of LCC on multivariate logistic regression analysis. Conclusion: Supplementation of LT4 should be carefully considered in premature infants with thyroid dysfunction and serial monitoring of blood pressure should be warranted if LT4 was administered.

Development of an ELISA for Quantitative Detection of Immunoglobulin G (IgG) and IgA Antibodies to Helicobacter pylori for Use in Korean Patients with H. pylori-Associated Diseases

( Ji Hyun Seo ),( Jin Su Jun ),( Hee Shang Youn ),( Jung Sook Yeom ),( Ji Sook Park ),( Chan Hoo Park ),( Hyang Ok Woo ),( Woo Kon Lee ),( Myung Je Cho ),( Kwang Ho Rhee ) 대한소화기학회 2013 Gut and Liver Vol.7 No.4

Background/Aims: We aimed to develop a quantitative enzyme- linked immunosorbent assay (ELISA) using whole-cell lysates of Helicobacter pylori 51 and to investigate its validity. Methods: Data from 300 plates were obtained by two different operators. Standard sera were used to make a standard curve to analyze the quantity of anti-H. pylori immunoglobulin G (IgG) and IgA antibody. We obtained reproducible data with fewer dilutions of samples by the addition of serially diluted standard serum to each ELISA plate. To evaluate the validity of this ELISA, the 114 H. pylori-positive and negative subjects were stratified into four age groups, i.e., 0 to 4, 5 to 9, 10 to 15, and 20 to 29 years, before testing. Results: The mean IgG-antibody titers in H. pylori-positive and -negative subjects were 1,766.4 IU/mL and 654.3 IU/mL (p<0.001). The mean IgA-antibody titers in H. pylori-positive and -negative subjects were 350.1 IU/mL and 193.5 IU/mL (p<0.001). Anti-H. pylori IgG and IgA titers in the four age groups were higher in H. pylori-positive subjects than in H. pylori-negative subjects (p<0.05). Conclusions: Using the current ELISA based on whole-cell lysates of H. pylori 51, reliable anti-H. pylori antibody titers were obtained regardless of the subject`s age. (Gut Liver 2013; 7:437-442)