도토리 추출물의 3T3-L1 세포 분화억제 효과

김지연,이진,이창원,김애정,Kim, Ji-Yeon,Lee, Jin,Lee, Chang-Won,Kim, Ae-Jung 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

본 연구에서는 만성질환의 주요 위험인자인 비만을 예방하기 위한 식품소재로서 3T3-L1세포를 이용하여 탈피한 도토리의 항비만 효과를 알아보고자 하였다. 3T3-L1 세포에서 생존율(MTT assay)을 측정한 결과, AE와 AW 시료 모두 $500{\mu}g/mL$ 농도에서는 다소 생존율의 감소를 보여, $300{\mu}g/mL$를 최종 농도로 정하였다. 3T3-L1 세포의 지질축적 억제 효과를 측정한 결과, 농도 $100{\mu}g/mL$로 처리하였을 때 두 시료 모두 지질축적량의 증가를 보였으나, $200{\mu}g/mL$ 처리농도에서 AE 시료는 82%로, AW 시료는 74%로 감소되다가 $300 {\mu}g/mL$ 농도에서는 두 시료 모두 약 53% 수준까지 지질축적이 억제되었다. 3T3-L1 세포에서 중성지방 억제 효과를 확인한 결과, AE 시료의 경우 $200{\mu}g/mL$ 농도에서 11%의 감소율, $300{\mu}g/mL$ 농도에서 42% 수준의 감소율을 보였다. AW 시료도 $200{\mu}g/mL$ 농도에서 5%의 감소율과 $300{\mu}g/mL$에서 41%의 감소율을 보였다. 3T3-L1 세포의 ROS 생성량을 측정한 결과, 시료 농도 $200{\mu}g/mL$에서 AE는 42%, AW는 33%로 $300{\mu}g/mL$에서는 AE는 58%, AW는 52%로 ROS 생성량의 억제를 보였다. 3T3-L1 세포에서 mRNA 발현에 미치는 영향을 대조군과 비교하였을 때 두 시료(AE와 AW) 모두 $300{\mu}g/mL$ 농도에서 $PPAR-{\gamma}$은 54%와 38%, aP2는 40%와 18% 수준의 발현을 억제시키는 것으로 나타났다. 이상의 결과로 볼 때 탈피한 도토리는 3T3-L1 세포의 분화를 억제함으로써 새로운 항비만 소재로의 가능성이 있는 것으로 판단된다. This study aimed to investigate the suppressive effect of acorn extracts, by evaluating 70% ethanol extract (AE) and hot water extract (AW) using 3T3-L1 preadipocytes. We applied various levels (0, 100, 200, 300 and $500 {\mu}g/mL$) of AE and AW to 3T3-L1 preadipocytes. The cell viability of the 3T3-L1 preadipocytes was not affected by up $300 {\mu}g/mL$ of extracts, but was suppressed by level $500{\mu}g/mL$ of both AE and AW by 20% and 9% respectively. The accumulation of lipid droplets in differentiated 3T3-L1 preadipocytes was dose-dependently suppressed by AE and AW. Especially, at high concentrations ($300{\mu}g/mL$), AE (42%) was more effective than AW (41%). Reactive oxygen species (ROS) was also dose-dependently suppressed by treatment with AE (58%) and AW (52%). With regard to the mRNA related to differentiated 3T3-L1 preadipocytes, $PPAR-{\gamma}$ and aP2 were suppressed by treatment with AE (54 and 40%) and AW (38 and 18%). From our results, acorn extract (AE) has more suppressive effects than AW in differentiated 3T3-L1 preadipocytes. We therefore concluded that acorn has suppressive effects against obesity in differentiated 3T3-L1 cells due to antioxidation.

항암화학요법 관련 오심과 구토 완화를 위한 비약물요법에 관한 연구동향

소향숙,최자윤,조인숙,김영재,김지영,김애숙,김옥미,김춘심,김현오,설영애,안정옥,이애리,이영자 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: Purposes of this study were to understand the current trends on complementary therapy in relieving chemotherapy-induced nausea and vomiting and to suggest the future research direction. Method: Subjects were selected on CINAHL, MEDLINE, Korean Academy Data Base from 1980 to 2001 which used nausea, vomiting, chemotherapy and complementary therapy as key words in experimental studies. Eight korean articles and twenty-one international articles were analyzed in terms of general characteristics, research methods, and types of complementary therapy. Data were analyzed by using descriptive statistical methods. Result: Since 2000, researchers have more actively used complementary therapy. In subject characteristics, mean age was 35.5 years old, 45% of the researchers were performed with high level of incidence of chemotherapy induced vomiting, 14% of them set limit of consecutive cycle during research, and 65% of them did not comment the selecting criteria of sample. About 60% of them were designed post-test only control group: 35% used INV by Rhodes, 31% used Likert scale, and 24% used VAS for dependent variable. Muscle relaxation therapy was mostly applied for relief of nausea and vomiting. Conclusion: Further studies will be needed to control extrinsic variables affecting nausea and vomiting in research design and to accumulate evidence with studies applying various complementary therapies.

정신분열병의 유전적 고위험군에서 인지, 정서 및 사회기능 연구

이경진,위휘,유소영,이애라,송지연,하태현,홍경수,김명선,권준수 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.1

Objectives : Vulnerability marker in biological relatives of schizophrenia may be very useful in identifying the propensity for the development of schizophrenia. This study suggests that the relatives at risk for genetically developing schizophrenia have impairments in several domains of cognition, social function and affective function as a vulnerability marker. Methods : A neurocognitive function test, a social function test and an affective function test were administered to fifteen healthy relatives from families with two or more patients with schizophrenia (geneticallly high risk subjects). Fifteen healthy controls matched for age and gender were recruited in this study. Results : Compared to the control subject, the genetic high risk subjects performed significantly more poorly in the selective attention and recall memory of neurocognitive function. The high dsk subjects also had lower scores in independence-performance and independence-competence of the social function test and emotion control of the affective function test. Conclusion : Selective attention, recall memory, independence-performance, independence-competence and emotion control ability may be a valuable marker for genetic study of schizophrenia.

Amplatzer Septal Occluder를 이용한 심방중격결손 폐쇄의 초기 성격

이형두,박지애 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

초등학교 민속무용 교육 과정에 관한 연구

이애현;강지영 한국무용연구회 2007 한국무용연구 Vol.25 No.1

This study is aimed to review whether the text book of Physical education in primary and high school has been focused on “The Continuity on education” and whether it has been doing the bridge role for proceeding to higher level dance education, by analysing in detail the chapter “Korean Folk Dance” in the 7’th education programme(text book). It was checked whether there is a certain continuity between the education target and the content of text book, while, the way for improvement was searched in order to maximize the education effect. The study is conducted also to provide the helpful information for the 8'th education programme. As a result of analysis, we first found that the education target of the primary and high school was not realistic, not constructive/ systematic according to grades and chapters and there were also no differences on the education target by grades. It was also known that the way of selection for folk dance programme was same in both text books while the target of education was not constructive without consideration of the learning progress of students and, therefore, the content was not enough/ suitable to fulfil the aim for art education value/ target. The detailed review shows that the content for Korean folk dance mainly deals with functions only without considering the interest of student and does not basically meet the principle specialities of Korean folk dance, I. e, “Gathering together and Enjoying with joy.” On the other hand, the high school text book takes the content of the same of primary school as it is, which was also contradicting to the education aim of “Various experiences” being emphasized in high school. The continuous repeats of simple content can eventually make the education (learning) will of student decreased and can further give just confusions to student on the concept of education by unmatching contents of books for different schools. Therefore, a certain guide line with clear principle must be given for the contents of text books in order to provide the continuity in between primary and high school while the remake/ modification of text book are required, so that it can provide systematic and constructive education, according to the learning procedure of students. 본 연구는 초·중·고등학교간의 연계적인 학습으로써 초등학교 체육 교과 내 무용교육의 가치에 역점을 두고 초등학교 3•4•5•6학년 『체육』 교과서 내의 '우리나라 민속 무속 단원을 중심으로 분석하였다. '우리나라 민속무용'단원0| 교과목표체계와 내용체계 면에서 연계성을 갖고 있는지에 대해 살펴보고, 학습자의 교육적 효과를 극대화시키는데 있어서의 문제점을 지적하여 개선방안을 모색하였다. 분석 결과, 초등학교 학년별·단원별로 학습목표가 비현실적이며 구체적으로 세분화 되어 있지 않았고, 학년별 목표의식의 차별성이 없음을 알 수 있었다. 구체적으로 제시하면 초등학교 교과서에는 동일한 민속무용의 프로그램이 중복 수록되어 있었고, 학습자의 발달과정을 고려하지 않은 목표 및 내용 선정, 그리고 단원의 소개에 그친 나열식의 교과 내용, 예술교육으로써의 가치와 목표에 부합되지 않는 내용 수록 등 문제점들이 지적되었다. 즉, 우리나라 민속무용의 교과내용은 학생들의 흥미를 고려하지 않은 기능중심의 교과 내용으로 편성되어 있으며. 우리나라 민속무용의 특성인 함께 어우러지고 흥겹게 즐기기 위한 목표에 부합되지 않는 것으로 나타났다. 따라서 초등학교 무용교육 과정간의 연계성을 갖도록 목표설정과 내용선정에 타당한 근거 및 기준점을 마련하기 위해 학습자의 발달상황에 맞는 단계적이고 체계화된 교과서의 개정이 요구되며 개정 방향은 다음과 같다. 첫째, 목표체계 면에서는 성장발달 단계에 따른 적합한 목표설정으로 학습자의 성취기준을 높여주며, 흥미위주의 교육내용으로 편성해야 할 것이다. 둘째, 내용체계 면에서는 다양한 프로그램의 개선0| 시급하며, 교육프로그램화 시킬 수 있는 민속무용의 발췌도 함께 이루어져야 한다. 따서 각 학년별로 단원별·차시별 목표와 내용에 적합한 기초·기본·전보의 세가지 학습단계를 적용한 학습체계가 마련되어야 할 것이다.

産業災害로 인한 手脂切斷 患者의 療養期間과 診療費의 變異

이종호,이종영,손지연,하영애,박순우,감신,이영숙,김건엽,강윤식 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.3

This study was conducted to analyze variation in patient days and medical care benefits among finger-amputated patients due to industrial injury. The 242 personal data on medical care for finger-amputated patients due to industrial injury(131 in 1994, 111 in 1995) of Regional Labor Office were analyzed. The major results of this study were as follows: Patient days per case were 69. And the ratio of the maximum and the minimum among patient days for case was 26.6. Patient days per case in university hospital were 134.8 and the longest than other medical facilites. The ratio of the maximum and the minimum among the patient days per case was higher in hospital(24.7) than in other medical facilities. Benefits per case were 1,258,000 Won. And the ratio of the maximum and the minimum among benefits of case was 232.0. Case benefits was higher in university hospital (2,685,000 Won) than in other medicl facilities. The ratio of the maximum and the minimum among the benefits per case was higher in hospital(232.0) than in other medical facilities. Variation in patient days and medical care benefits per finger-amputated patient in industrial injury was large. This study results indicated need for a new industrial injury compensation insurance policy initiative for efficient utilization of medical resources and quality assurance. And the author thought that the results of this study would influence the policy for the industrial injury compensation insurance policy.

1995년도 ABO식 혈액형 감정에 의한 사건별 분포에 관한 분석

이영보,서지석,이영애,최상규 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

실외놀이에서 유아의 또래갈등 원인과 해결전략, 이후의 놀이변화

이지선,엄정애 이화여자대학교 교육과학연구소 2022 교육과학연구 Vol.53 No.1

본 연구는 유아들의 실외놀이에서 발생하는 또래갈등 원인과 또래갈등 해결전략, 이후의 놀이변화 탐색을 목적으로 진행되었다. 연구자료는 강원도에 위치한 다은유치원 만 5세 정운반의 유아 18명(남아 7명, 여아 11명)과 교사 1명을 연구 참여자로 하여 총 21회의 참여관찰 및 면담을 통해 수집되었다. 연구결과, 첫째, 실외놀이에서 유아들의 또래갈등 원인은 물리적 원인과 관계적 원인으로 양분되었다. 물리적 원인은 고정놀이기구 선점권과 한정된 놀잇감 소유권, 놀이구성물 훼손으로 나타났으며 관계적 원인은 지식과 경험에 대한 의견충돌, 상대가 원하지 않는 장난, 신체적 접촉으로 나타났다. 둘째, 유아들이 실외놀이에서 발생한 또래갈등을 해결하기 위해 사용한 갈등해결전략은 일방향적인 해결전략, 상호적인 해결전략, 제3자 개입전략으로 나타났다. 마지막으로, 갈등해결전략 사용 후 유아들의 놀이변화는 놀이 확장, 놀이틀로 복귀, 놀이소멸로 나타났다. 놀이틀로 복귀는 완전한 갈등해결을 통한 놀이복귀와 불완 전한 갈등해결을 통한 감정 표출하기의 하위범주로 분류되었다. 본 연구는 ‘실외놀이’라는 공간에서 발생한 또래갈등의 전 과정을 총체적으로 분석하여 또래갈등 연구에 대한 스펙트럼을 넓힐 수 있을 뿐만 아니라 또래갈등의 시작과 전개, 종결과 이후 상황에 이르기까지의 전 과정에 대한 이해를 도모하고, 교사의 개입과 역할에 대해 다시 생각해 볼 수 있는 계기를 마련하였다는 점에서 의의가 있다. This study was conducted with the purpose of exploring the causes of peer conflict, peer conflict-solving strategies, and subsequent changes in play that occur in the outdoor play of young children. The research data were collected through a total of 21 participatory observations and interviews with 18 young children (7 boys and 11 girls) and 1 teacher in the 5-year-old Jeong-un class at Daeun Kindergarten located in Gangwon-do, Korea. As a result of the study, first, the causes of peer conflict among young children in outdoor play were divided into physical causes and relational causes. Physical causes were the preoccupation with play equipment, ownership of a limited amount of toys, and damage to play components. Relational causes were conflicts of opinion on knowledge and experience, and unwanted mischief and physical contact. Second, the conflict-solving strategies used by young children to solve peer conflicts that occurred in outdoor play were one-way resolution strategies, mutual resolution strategies, and third-party intervention strategies. Finally, after using the conflict-solving strategies, the changes in play of young children were found to be expansion of play, return to play framework, and extinction of play. Return to play framework was classified as a subcategory of returning to play through complete conflict resolution and expressing negative emotions through incomplete conflict resolution. This study can help broaden the scope of peer conflict research by comprehensively analyzing the entire process of peer conflict that occours in outdoor play. It is also meaningful in that it provides an opportunity to rethink the teacher’s role in conflict resolution.

특발성 과호산구 증후군 환자의 치료 도중 발생한 간혈종 1례

이영태,김동기,박지훈,김윤정,김기향,박보민,권민정,김애란,이원식,주영돈,손창학 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Idiopathic hypereosinophilic syndrome is characterized by marked elevation of eosinophil count ( >1,500/㎣) in peripheral blood without explaining etiology. It shows tissue involvement in many organs such as liver, G-I tract, myocar dium, lung, skin, kidney and nerve. Some case of hypereosinophilic syndrome with hepatic involvement have been reported, but not much. We have recently experienced a case with idiopathic hypereosinophilic syndrome of a 40 year old man who presented with RUQ pain. By abdominal CT, ill- defined subcapsular hematoma was detected. Liver biopsy showed eosinophilic infiltration within the portal tract and sinusoid. Drain procedure and corticosteroid adminiiatbrought marked improvement of clinical manifestation, hematologic abnormality and radiologic abnormality.

우울증 환자의 부모양육행동, 대상관계 및 자아존중감에 관한 연구

최지애,최영민,이정호,이동우 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.5

Objectives : The aim of this study was to examine the relationship between the Object Relations, Parenting Behaviors, and Self-esteem with Depression. Methods : This study included 60 Depression patients and 90 healthy individuals. Parent Behavior Inventory (PBI), Bell Object Relation Inventory (BORI), Self-Esteem Scale (SES) and Beck Depression Inventory (BDI) were used for measuring parenting behaviors, object relations, self-esteem and severity of depression in orderly. Results : Depression was negatively associated wi self-esteem, and positively associated with four subscale of Object relation. Also, depression was positively associated with maternal intrusiveness, Parental negligence and negatively associated with maternal affection. The most potent variable to explain depression was self-esteem, and the second was insecure attachment. Maternal affection was the most explainable variable of self-esteem, and the most potent variable of insecure attachment was maternal intrusiveness. Correlation analysis with insecure attachment and self-esteem as control variable individually, there was weak correlation among variables. Conclusion : This study shows object relation, parent behavior, and self-esteem are related with depressive symptoms. Object relation (especially, insecure attachment) and self-esteem is more important and proximal cause in depression than Parental behavior. Result of the present study indicated that inadequate parental behavior m childhood foster insecure object relation and low self-esteem, then they contribute to depression through their interaction.