Salicylaldehyde-Acetonitrile 非水溶媒中에서 亞鉛의 直流 Polarograph定量法

黃在順,黃三榮 全北大學校 基礎科學硏究所 1985 基礎科學 Vol.8 No.1

A method for the direct current polarographic determination of metallic zinc dissolved directly in organic solvent, salicylaldehyde, as metal-solubility in salicylaldehyde acetonitrile and 0.2M LiClO_4 supporting electrolyte, was studied. The solution of zinc was taken into a cell. After dried nitrogen gas was passed through it for 20 min. the direct current polarogram in the region of applied potential from 0.0-1.0V(vs. SCE) was recorded and all experiments was carried out at 25.0 ± 0.5℃. A reversible polarographic reduction wave was obtained and the reduction half wave potential was-0.56V(vs, SCE). Zinc was determined by refering to the calibraton curve.

Ethylacetoacetate-Cadmium 錯體의 Polarograph的 硏究

黃在順,黃三榮 全北大學校 1984 論文集 Vol.26 No.-

Polarographic behavior of Cadmium-Ethylacetoacetate Chelate in the methyl alcohol and 0.3M NaClO_4 supporting electrolyte was studied in detail. The electrode reaction was found to be a reversible polarographic wave corresponding to two electron reduction and diffusion controlled, and reduction half wave potential was -0.59V(vs. SCE) in this case, assuming that metal complex Cd(EAA)_2 is reduced reversibly with two electron transfer, the electrode reaction is given as follows Cd^+2(EAA)n+2e ↔Cd+nEAA

사마귀류 추출물의 생물학적 활성 비교

허진철,황재삼,강석우,윤치영,이상한 慶北大學校農業科學技術硏究所 2007 慶北大農學誌 Vol.25 No.-

In order to investigate the availability of insect resources for agrobiotechnological or medical purposes, we examined antioxidant (DPPH and FRAP assay) and cell viability by oxidant stress and NO inhibition assay by treatment of the extracts of Statilia maculata Thunberg, Tenodera angustipennis Saussure and Tenodera aridifolia Stoll. We found that Tenodera angustipennis Saussure and Tenodera aridifolia Stoll extract (DW, etanol, methanol) had high levels of anti-oxidant activity, whereas Tenodera aridifolia Stoll extract showed increased cell viability by hydrogen peroxide and inhibition of NO production. These findings suggest that Statilia maculata Thunberg, Tenodera angustipennis Saussure and Tenodera aridifolia Stoll, extract have potentials to be developed for agrobiotechnology or medicinal use, indicating that mechanistic study including inhibition against molecular inflammation will show a possibility for the development of useful insect resources.

선인장 양심실 보조장치의 설계 및 임상적용을 위한 평가

민병구,박찬영,최재순,이혁수,황창모,김삼성,윤걸중,김종원,선경,이경갑,정종태,김원곤 제주대학교 인공심장이식연구소 2001 인공심장 연구 Vol.2 No.1

현재 개발되고 있는 맥동형 이식형 인공심장은 완전이식형 인공심장과 좌심실보조장치 뿐이다. 좌심실보조장치를 장착한 환자의 10~15%가 우심실의 보조를 필요로하고 완전이식형 인공심장의 장착을 위해서는 자연심장을 제거해야하는 상황에서 이식형 양심실보조장치에 대한 필요성이 대두되고 있다. 본 연구진은 완전이식형 인공심장으로 개발된 한국형 인공심장을 개선하여 양심 실 보조장치를 개발하였다. 양심실보조장치는 이동형 작동기식 미케니즘을 이용하고 있으며 에너지 변환장치와 감속기로 구성된 작동기와 혈액주머니, 그리고 내장형 제어기로 구성되어 있다. 선인장 펌프로 명명된 KAH350은 캐뉼라의 연결을 위한 커넥터를 포함하여 길이 177mm, 폭 164mm, 높이 67 이고 무게 780g이며 최대 심박출량은 5L/min 이다. 양심실보조장치로 개발된 선인장펌프는 좌심실보조장치로도 응용이 가능하다. 이식적합성과 생체적합성을 평가하기 위하여 좌심실보조장치로 5회, 양싱실보조장치로 6회의 동물실험을 수행하였다. 각각 최장 28일간 생존하였으며 11회의 동물실험중 장치의 결함에 의한 것이 3회 있었는데 이들은 모두 전자장치의 결함과 방수처리문제였다. 현재 이식적합성과 일박출량을 향상시킨 KAH400모델이 개발중이다. The types of pulsatile implantable artificial hearts that are on the way of development and have been developed are totally implantable artificial heart and implantable left ventricular assist device (LVAD). Approximately 10% to 15% of all patients Implanted with wearable VADs have required right heart support with another device. And it is reluctant to patient who should remove his or her own heart to be implanted with total artificial heart. These situations drive the development of implantable bi-ventricular assist device (BVAD). The Korean BVAD was develophed by modifying the moving actuator type Korean artificial heart. This electro-mechanical BVAD comprises actuator including energy converter and reduction gear train, blood sacs, and internal motor and energy controllers. The KAH350 which is named as 'Cactus Pump' is 177mm in length, 164mm in width, and 67mm in thickness including connectors and nuts. The weight and maximum cardiac output of Cactus Pump is 780g and 5L/min, respectively. The Cactus Pump that was developed as BVAD could be used as LVAD by attaching compliance caps on the 2 ports of one ventricle. The animal experiments were undergone 5 times for LVAD, and 6 times for BVAD. The best records were 28 days survival in both applications. There were 3 times of device failure and they were all associated with electrical connection and hermetic sealing. The KAH400 that has improved anatomical fitting characterisitcs and stroke volume is on development.

RAPD-PCR을 이용한 누에 계통간 유연관계분석

강은영,김성근,이진성,황재삼,서동상 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.1

We have analyzed the genetic relationship among the silkworm (Bombyx mori) strains using RAPD-PCR. A total of 45 polymorphic RAPD markers amplified from 9 different primers were used to analyze the genetic relationship among the seven silkworm strains. The genetic similarity coefficients among the seven silkworm strains were estimated using the RAPD markers by UPGMA method. The genetic similarity coefficient between 127 and CS120 was 0.261. This value was lower than any other genetic similarity coefficient among the strains tested. And 127-specific RAPD band patterns were observed in 3 primers from 9 polymorphic primers used. This study makes possible phylogenetic identification among the Bombyx mori strains in the molecular level using RAPD-PCR techniques.

Research Articles : Molecular Characterization of a Defensin-Like Peptide from Larvae of a Beetle, Protaetia brevitarsis

( Jae Sam Hwang ),( Bo Ram Kang ),( Seong Ryul Kim ),( Eun Young Yun ),( Kwan Ho Park ),( Jae Pil Jeon ),( Sung Hee Nam ),( Hwa Jin Suh ),( Mee Yeon Hong ),( Ik Soo Kim ) 한국잠사학회 2008 International Journal of Industrial Entomology Vol.17 No.1

A cDNA encoding a defensin-like peptide (Protaetiamycine) from the larvae of a beetle, Protaetia brevitarsis was cloned. The DNAs encoded the deduced propeptide of 79 amino acid residues with the predicted molecular weight of 8.4 kDa and PI of 8.24. Overall amino acid sequence of this protein has 39% similarity to that of Rhodnius prolixus defensin, 43% similarity to that of Acalolepta luxuriosa defensin, and 72% similarity to that of Oryctes rhinoceros defensin, suggesting that this gene is an insect defensin. In an attempt to apply the anti-bacterial peptide to the development of therapeutic agents, a 12-mer peptide amidated at its C-terminus, ACAAHCLAIGRG-NH2 (Ala55-Lys66-NH2, 12Pbn) was synthesized. This peptide showed some antifungal activity against Candida albicans. To increase antifungal activity, six 9-mer peptides were synthesized by modifying amino acid sequences of 12Pbn fragment. Among these peptides, 9Pbm3-9Pbm6 exhibited strong activity compared with Cecropin B and mellitin.

Scolopendrasins: new antimicrobial peptides from the centipede, Scolopendra subspinipes mutilans

Jae-Sam Hwang,Joon Ha Lee,Minchul Seo,Minhee Baek,Hwa Jeong Lee,In-Woo Kim,Sun Young Kim,Mi-Ae Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

We have analyzed the transcriptome of Scolopendra subspinipes mutilans using RNA sequencing and identified severalantimicrobial peptide candidates. Among the peptides, named scolopendrasins, were selected based on the physicochemicalproperties of antimicrobial peptides via an in silico analysis. As a result, we evaluated the antimicrobial activities ofscolopendrasins against Gram positive and negative bacteria including Candida albicans by radial diffusion assay and colonycount assay. We also investigated the cytotoxicity of scolopendrasins through hemolysis assay. We found that the actionof scolopendrasins involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides,lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results will provide a basisfor developing therapeutic agents such as peptide antibiotics.

Selection of Differentially Expressed cDNA Clones during Bombyx mori Embryogenesis

Hwang Jae-Sam,Go Hyun-Jeong,Goo Tae-Won,Yun Eun-Young,Seong Su-Il,Kim Ik-Soo,Ahn Im-Young,Lee Pyeong-Jae,Kang Seok-Woo 한국응용곤충학회 2004 한국응용곤충학회 학술대회논문집 Vol.2004 No.1

Development of novel foods and functional materials from insects

Jae-Sam Hwang 한국산업식품공학회 2022 식품과 기계 Vol.19 No.1

Isolation and characterization of the novel antibacterial peptide “Coprisin” from a dung beetle, Copris tripartitus

Jae-Sam Hwang,Yeon-Ju Kim,Hea-Son Bang,Eun-Young Yun,Seong-Ryul Kim,Kwan-Ho Park,Hwa Jin Suh,Bo-Ram Kang,Iksoo Kim 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

To find some antibacterial peptides responsible for bacterial resistance, we performed differential hybridization with total cDNA probes which synthesized from normal and immunized larvae. Thirteen individual cDNA transcripts were expressed differentially in a total 1,862 random cDNA clones. One of upregulated genes is a novel member of the insect defensin-like peptide(Coprisin), a family of antibacterial peptide. Northern blot analysis showed that Coprisin was up-regulated at 4h and reached the highest point level at 16h after injection of E.coli. The deduced amino acid sequence of Coprisin was composed of 80 amino acids with predicted molecular weight of 8.6 kDa and PI of 8.72. Comparison of the deduced amino acid mature portion of Coprisin with defensin-like peptide of other insect indicated that it has 79.1% and 67.4% identity with Anomala cuprea and Allomyrina dichotoma, respectively. To find antibacterial active region of Coprisin, we synthesized four peptides corresponding to amino acid residues 1V-43N-NH2(CopN1), 5-16(CopN2), 19-30(CopN3) and 31-43(CopN4) of coprisin having amidated amino acid residues at their Cterminal. A 12-mer amidated at its C-terminus, ACALHCIALRKK-NH2 (Ala19-Lys30-NH2) was synthesized based on the deduced amino acid sequence, assumed to be an active site sequence. This peptides showed antibacterial activity against E.coli, Staphylococcus aureus, MRSA, Psedomonas syringae, and Pectobacterium carotovorium. Modified 9-mer peptide, LRCIALRKK-NH2, showed strong antibacterial activity than mellitin peptide used as a positive control against gram-negative and gram-positive bacteria. This peptide showed no haemolytic activity and quite stable at 100℃ for several hours of incubation and in a wide pH range(pH2-12). Therefore, this peptide may be a good candidate for the development of new drug with potent antibacterial activity without cytotoxicity.