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Hong‑Jai Lee,Bo‑Young Shin,Jae‑Seung Moon,Ailyn Fadriquela,Selikem Abla Nuwormegbe,Chun‑Chang Ho,Jin‑Su Shin,Jee‑Sang Yoon,Sang‑Kyou Lee,Soo‑Ki Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3
Background Lack of universal replication system for hepatitis B virus with narrow host range and organ tropism has hampered to uncover the pathogenesis of HBV. Previously, we reported the essentiality of humoral milieu and its components toward HBV and hepatitis C virus survival/viability in vitro. Of these components, the precise role of enterohepatic humoral milieu such as bile acid (BA) on HBV cultivation in vitro and in vivo is unknown. Objective We explored whether BA, specifically taurochenodeoxycholic acid (tCDCA) would directly regulate the viral DNA and surface antigen expression of HBV in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. Result We found that higher concentration of taurochenodeoxycholic acid (tCDCA) is able to preserve the genomic stability of HBV in cell-free DMEM, showing higher the surface antigenicity than taurocholic acid (tCA). In line, we found that in vitro cell culture condition (100 μmol/L of tCDCA coupled with 1 × 108 g e/mL HBV) would be optimal for HBV entry into target cells. Using this, human (HepG2, Huh7), and rodent (Hepa1c1c7, H4-II-E) hepatoma cell lines were infected by HBV, as evidenced by the presence of HBV biomarkers (HBsAg, and HBV DNA in culture supernatant, as well as HBcAg in cell). Further, cellular entry test revealed that HBV is able to infect 12 different non-hepatic cell lines regardless of species, and organ/tissue, consequently reproducing progeny as confirmed by HBV biomarkers. Last, reinfection test showed that the progenies of HBV from immortalized HepG2, and Hepa1c1c7 cells are able to enter into each or vice versa naïve HepG2, and Hepa1c1c7 cells with or without BA. Conclusion This study demonstrates that enterohepatic humoral milieu such as BA, specifically tCDCA would directly regulate HBV DNA and its surface antigen expression in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. This is the first note to render HBV permissive to human or rodent hepatic and non-hepatic cells via sole manipulation of humoral milieu, thus establishing the platform for in vitro robust replication system of HBV.
노래부르기 음악프로그램이 정신질환자의 자아존중감과 대인관계에 미치는 영향
홍상희,윤지원,이재금,김지연 병원간호사회 2006 임상간호연구 Vol.11 No.2
Purpose: The objective of this study was to verify the effects of the Singing program for psychiatric patients on their self-esteem and interpersonal relationship, and to provide data for the nursing intervention that is necessary for making their life better. Method: The subjects were 10 in-patients with psychotic disorders at C university hospital in Seoul, from july 3 to September 18 im 2005, and the methodology was one group pre experimental- post experimantal design. The instrument for the measurement of self-esteem is Rosenberg Self-Esteem Scale(1973), which was translated by Chun Byung Jae(1974) and the measurement of interpersoral relationship is Schleim & Guerney Relationship Change Scale(1971) which was translated by Mun Seok Mo(1980) and revised by Chun Seok Gyun(1994). SPSS program was used for the analysis of the collected data, which were analyzed by the paired t-test and one-way ANOVA. Result: Singing therapy had an effect on improvement of the self-esteem, and seemed to be more effective as the frequency of the program increased. Singing therapy had an effect on improvement of the interpersonal relatinship, and seemed to ne more effecive as the frequency of the program increased. Comclusion: Singing therapy is found out to be effective program affecting the improvement of both the self-esteem and interpersonal relationship of the psychiatric patients. Therefore the nurses can use the Singing therapy as the nursing intervention for imtrovement of the self-esteem and interpersonal relationship of the psychiatric patients in clinical practice.
서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)
김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1
In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.
김재홍,김한중,노영석,김영태,김중환,황규홍,이정덕,백승철,김건우,조상현,김준영,김석민,김영호,김상순,이승한,김방순,전덕규,하상근 대한화학요법학회 1992 대한화학요법학회지 Vol.10 No.2
The prevalence of PPNG among pretreated gonorrhoeae cases isolates at the VD clinic of Choong-ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1990, 162 strains of N.gonorrhoeae were isolated, among which 80 were PPNG. The prevalence of PPNG in Seoul showed increased tendency till 1987, thereafter, it has been stationary.
봉독약침이 류마티스 관절염 환자의 기능회복 및 삶의 질에 미치는 영향
이상훈,이현종,박상민,김수영,박재경,홍승재,양형인,이재동,최도영,김건식,이두익,이윤호 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-
Objective: To evaluate the effects of bee venom acupuncture(BVA) on the rehabilitation and quality of life in rheumatoid arthritis(RA) patients Methods: Patients with RA were treated with the BVA therapy twice a week for 3 months. Tender joint counts, swollen joint counts, morning stiffness, Erythrocyte Sedimentation Rate(ESR), C-reactive protein(CRP), patient global assessment, physician global assessment, Korean health assessment questionnaire(KHAQ) were estimated and analyzed before and after BVA therapy. Results: Tender joint counts, swollen joint counts, morning stiffness showed significant decrease after BVA therapy. But, as acute inflammatory reactants, ESR showed no significant difference and CRP showed significant increase after BVA therapy. Patient global assessment physician global assessment, and KHAQ index showed significant improvement after BVA therapy. Conclusions: BVA therapy can improve rehabilitation and health-related quality of life RA patients as well as clinical symptom and signs. Further study is required in more population with large scale including acute inflammatory reaction of BVA therapy.
서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)
김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2
Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.
姙産婦 및 胎兒 臍帶血中의 Somatomedin-C와 成長 호르몬値에 關한 硏究
洪瑞裕,姜宰成,洪性鳳 고려대학교 의과대학 1989 고려대 의대 잡지 Vol.26 No.1
The mechanism responsible for intrauterine fetal growth is poorly understood despite the impressive progress made in understanding how hormone and growth factors regulate postnatal growth Recently the isolation of the insulin-like growth factor Ⅰ and Ⅱ shed some light on this important subjects and insulin-like growth factor Ⅰ is similar to the somatomedin-C in structuies. It has been shown that the growth hormone may augment the action of the insulin on the liver to form somatomedins which might augment fetal growth. This study was undertaken to determine the relationship between growth hormone and somatomedin-C in mothers and their infants, and to evaluate the correlation of these hormones to birth weights in newborn infants in 45 term pregnant women and their infants, and 10 non-pregnant healthy women. The results were as follows; 1. Maternal plama growth hormone levels were significantly higher in non-pregnant women than women who delivered average for gestational age, small for gestational age and large for gestational age infants and the birth weights were not affected by maternal plasma growth hormone. 2. Maternal plasma somatomedin-C levels were significantly higher in pregnant women who delivered average for gestational age, small for gestational age and large for gestational age infants than non-pregnant women and the birth weights were influenced by maternal plasma somatomedin-C. 3. Fetal cord plasma growth hormone levels were significantly higher in small for gestational age infants than average or large for gestational age infants. 4. Fetal cord plasma somatomedin-C levels were significantly lower in small for gstational age infants than average or large for gestational age infnats. The birth weights were correlated to fetal cord plasma somatomedin-C. The present study suggests that the birth weights of all infants studied had a significant overall effect on maternal and fetal somatomedin-C and somatomedin-C may be involved in fetal growth in utero.
벼 무논골뿌림栽培時 播種期 및 立毛數가 生育 및 收量에 미치는 影響
洪性澤,朴栽成,朴成圭,李尙榮,池在浚,孫錫龍 충북대학교 농업과학기술연구소 1999 農業科學硏究 Vol.16 No.-
This study was conducted to establish the cultural techniques of the puddled-soil drill seeding of race (Oryza sativa L.), which is one of the efficient labor saving cultural methods, in the central part of Korea. Seoanbyeo was seeded at three times from April 10 to May 20 with the 20 day interval in 1994. The number of seedling stands per m2 at each seeding date were 50, 90, 130 and 170. The tillering characteristics, lodging characters, dry matter production, yield components and yield were investigated. The number of days to the maximum tillering stage was reduced at the later seeding date. It was 70, 55 and 25 days in seedings on April 10, April 30 and May 20, respectively. The critical effective tillering stage was also shortened at the delayed seeding. The higher the number of seedling stands was, the higher number of tillers, the shorter duration to the maximum tillering stage and the earlier critical effective tillering stage were resulted. The number of tillers was higher at the earlier seeding date. The number of tillers was increased in the order of 50, 90, 130 and 170 seedling stands per m2. The ratios of effective tillers were 53, 61 and 63% at seeding on April 10, April 30 and May 20, respectively. The ratios were not significantly different at April 10. But the ratios in seeding on April 30 and May 20 were reduced as the number of seedling stands was increased. The weight of stem base related to lodging was the highest with 2.01g/10 plants in the optimum seeding on April 30, while it was 1.59g/10 plants in the late seeding on May 20. It was also decreased as the number of seedling stands was increased. The dry weights at 30 days before the heading stage were increased at the later seeding, while the dry weight at the heading stage was the highest at seeding on April 30 which is considered as the adequate seeding time. The dry weights at 30 days before the heading stage were increased as the number of seedling stands was increased and they were increased up to 130 seedling stands per m2 at the heading stage. The length of panicle exsertion was shortened at the later seeding. The length of panicle exsertion was increased up to 130 seedling stands. but it was shorter at 170 seedling stands. The heading dates were later at seeding on April 30 and May 20 than at seeding on April 10. They were not remarkedly different among seeding dates at 50 seedling stands, it was earlier at the higher number of seedling stands over 90. The number of spikelets per panicle was decreased at the higher number of seedling stands. The ratio of ripened grains was higher at earlier seeding than at later seeding. It was not significantly different among the number of seedling stands at the same seeding time. Yields at seeding on April 30, April 10 and May 20 were 644, 624 and 551kg/10a, respectively. The seeding on April 30, the optimum seeding time, resulted the highest yield. Rice quality was decreased due to the increase of the green kerneled rice as the seeding date was delayed.