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方在旭,崔慧雲 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-
Cytogenetic analyses were done in thirteen natural populations of Scilla scilloides complex collected from Chejudo. Six genotypes, BB (2n=18), ABB (2n=26), AABB (2n=34), ABBB (2n=35), AABBB (2n=43) and AAABBB (2n=51) were observed, where A and B denote genomes with χ=8 and χ=9, respectively. Genotypes BB, ABB and AAABBB were found for the first time in Chejudo populations. Genotypes BB and ABB were never reported from the Korean populations. Six of the populations investigated were mixed populations of two or three cytogenetic types, and others were pure populations consisting of BB (1), AABB (4) and AABBB (2). AABB genotypes were predominant over other genotypes and AABBB was the next. AA genotypes growing in the mainland of Korea was not found. It was suggested that the Chejudo populations of S. scilloides complex were closely related to the Japanese populations than mainland populations of Korea. The number of B-chromosome ranged from one to four. The B-chromosomes were all isochromosomes (F) and no chromosome fragment (f) was found. The frequencies of B-chromosome were 75% in BB and AABBB genotypes, respectively. The frequency of B-chromosome in AABB plants was similar to that of AABB plants reported in the mainland populations.
최은정,방현,임주혁,이재홍 대한치매학회 2002 Dementia and Neurocognitive Disorders Vol.1 No.1
Extrapyramidal signs (EPSs) frequently accompany Alzheimer's disease(AD). However, the pathogenesis or pathologic basis for the EPSs in AD remains unclear. We report a case of presenile onset progressive dementia later associated with hemiparkinsonism. Brain biopsy showed neurofibrillary tangles and amyloid plaques. compatible with Alzheimer's disease. There are a few report about dopamine transporter imaging of AD with EPSs. 1-123 IPT SPECT revealed a decreased uptake in both basal ganglia, more severe on the left side and in the caudal putamen, which is consistent with the finding of idiopathic Parkinson's disease. Alzheimer's disease and Parkinson's disease are thought to be coexistent in our patient.
호밀 (Secale cereale L.) Esterase 동위효소의 유전적 연구
방재욱,최혜운 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-
Esterase isozymes (EST) in endosperm, root and shoot of rye seedlings were determined using polyacrylamide gel electrophoresis and the effect of B-chromosomes (Bs) on EST zymogram phenotypes was studied. The number and staining intensity of EST bands vary depending on the tissues applied. EST-A is present in all the tissues of the rye seedlings, where as EST-B is revealed as the coleoptile-specific esterase. EST-C exhibits a two-banded phenotype. EST-D showed different band patterns among tissues. In leaf EST patterns of rye with Bs and without Bs, EST-D showed difference in pattern wtith the presence of Bs. Individuals without Bs have two specific EST bands, whereas plants with 2Bs and 4Bs have three and four specific EST bands respectively. The results reported in this paper lend evidence for the genetic effect of Bs on EST patterns in rye.
方在旭,崔惠雲 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-
A karyotype of cytotype BB plant in Scilla Complex was established and the frequency of B-chromosomes were investigated. Chromosome complements of BB genome were composed of five pairs of subtelocentric and four pairs of metacentric chromosomes. Chromosome 1 has satellite with nucleolar organizer. Polymorphism was found in chromosome 2. The karyotype of cytotype BB will be available for analysis of genome composition in various cytotypes of S. scilloides Complex. The frequency of B-chromosome was 78.6%. Numbers of B-chromosome ranged from 1 to 4 and plants with 2B-chromosomes were predominant (57.2%). Two types of B-chromosomes, F and F', were found; F is a large iso-chromosome and F' a small one.
해조 원형질체 분리를 위한 한국산 조식동물에서의 세포벽 분해효소의 개발 2.명주고둥 아세톤분말의 Alginate lyase 효소안정성에 대하여
전방욱,최재석,최창근 강릉대학교동해안지역연구소 1997 東海岸硏究 Vol.8 No.1
Alginate lyase from Chlorostma xanthostigma was stabilized in vitro by optimizing buffer ingradients. The extraction buffer consists of 25mM acetate, pH 5.5, 20mM MgCl₂, 100mM NaCl and 3mM PMSF. The alginate lyase extracted with this buffer gave a decay rate of less then 1% per hr at 30℃ for at least 6h. In vitro instability of alginate lyase may be due to proteolytic degradation.
박재인,연방희,최태호,이은경,임선희,김홍은,조남석,김태동 忠北大學校 農業科學硏究所 2003 農業科學硏究 Vol.20 No.-
In order to establish a dyeing method with Magnoliaceae tree species dyeing was examined with 4 different parts, leaf, stem bark, root, flower of 4 Magnohaceae species, Magnolia kobus Dc., Magnolia denudata Desr, Magnolia obovata Thunb, Liriodendron tulipifera L Mordants used were chemicals, AIK(SO_4)_2 24H_2O, Ca(OH)_2, FeSO_4 7H_2O. All four species showed similar coloring among four different parts. Leaves and petals showed dark yellow, stem bark, grayish yellow, root bark, dark red or yellow. Al mordant made more brighter colour than Fe mordant. Stem bark showed less difference in color by mordants, whereas petals did the biggest.
류재천,최윤정,김연정,김형태,방형애,송윤선 한국환경독성학회 1999 환경독성보건학회지 Vol.14 No.1
Recently, several new methods for the detection of genetic damages in vitro and in vivo based on molecular biological techniques were introduced according to the rapid progress in toxicology combined with cellular and molecular biology. Among these methods, mouse lymphoma thymidine kanase (tk) gene forward mutation assay, single cell gel electrophoresis (comet assay) and transgenic animal and cell line model as a target gene of lac I (Big Blue) and lac Z (Muta Mouse) gene mutation are newly introduced based on molecular toxicological approaches. The mouse lymphoma tk^(+/-) gene assay (MOLY) using L5178Y tk+i- mouse lymphoma cell line is one of the mammalian forward mutation assays, and has many advantages and more sensitive than hprt assay. The target gene of MOLY is a heterozygous tk^(+/-) gene located in 11 chromosome, so it is able to detect the wide range of genetic changes like point mutation, deletion, rearrangement, and mitotic recombination within tk gene or deletion of entire chromosome 11. The comet assay is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakages in mammalian cells. Also, transgenic animal and cell line models, which have exogenous DNA incorporated into their genome, carry recoverable shuttle vector containing reporter genes to assess endogenous effects or alteration in specific genes related to disease process, are powerful tools to study the mechanism of mutation in vivo and in vitro, respectively. Also in vivo acridine orange supravital staining micronucleus assay by using mouse peripheral reticulocytes was introduced as an alternative of bone marrow micronucleus assay. In this respect, there was an International workshop on genotoxicity procedure (IWGTP) supported by OECD and EMS (Environmental Mutagen Society) at Washington D, C, in March 25-26, 1999. The objective of IWGTP is to harmonize the testing procedures internationally, and to extend to finalization of OECD guideline, and to the agreement of new guidelines under the International Conference of Harmonization (ICH) for these methods mentioned above. Therefore, we introduce and review the principle, detailed procedure, and application of MOLY, comet assay, transgenic mutagenesis assay and supravital staining micronucleus assay.
게놈 유형이 다른 무릇(Scilla scilloides comlpex)에서 FISH를 이용한 인 우성 다양성 분석
이상은,최혜운,방재욱 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.2
Nucleolar dominance, an epigenetic phenomenon in which one parental set of ribosomal RNA gene is silenced in an interspecific hybrid, was investigated in different cytotype plants of Scilla scilloides complex; AA, BB, AABB, ABBB, BBBB and AABBB. To determine the loci of the nucleolar organizer regions (NORs) in metaphase chromosomes, physical mapping of 45S rRNA gene (3.8 Kb) using FISH method was applied. Two signals of the 45S rRNA gene loci were observed in both the cytotypes of AA (2n=16) and BB (2n=18). Four signals were found in tetraploid BBBB, while two and three signals were found in cytotypes of AABB and ABBB, respectively. However, AABB plants in the population of Daegukhul-do Island had four NORs in both the A and B genome. Four signals were found in pentaploid AABBB plants. The different numbers of 45S rRNA gene signals suggested the phenomenon of nucleolar dominance or amphiplasty among different cytotype plants of S. scilloides. It is speculated that inactivation of NORs on a2 chromosome in the cytotype AABB plants is the result of the amphiplastic suppression between A and B genomes.