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A Metaviromic Analysis of Viral Communities in the Feces of Unexplained Acute Gastroenteritis
Baek, In Hyuk,Kim, Song Ah,Kim, Jong-Hwa,Park, Hee Kuk,Kim, Wonyong The Korean Society for Microbiology 2013 Journal of Bacteriology and Virology Vol.43 No.4
Although viruses are the most common cause of acute gastroenteritis (AGE) in humans, details about the causative viruses in AGE are largely unknown because many causative viruses are unable to be cultured by current culture techniques. In our study, fecal samples from 10 children under five years of age with unexplained AGE and 10 healthy children were investigated for RNA viruses using random priming (RP)-mediated sequence-independent single primer amplification (SISPA). The causative viruses in cases of cryptogenic diarrhea were then assessed for their potential diagnostic value. Of the 1,129 viral clones identified, rotavirus was most commonly associated with AGE (125 sequences, 22.4%). In contrast, bacteriophage was most common (43 sequences, 13.6%) in healthy children. The remaining 515 viral clones were unidentifiable. These findings suggest that investigation of cases or outbreaks of unexplained diarrhea using a metaviromic strategy is a new avenue for diagnosis.
Baek, In Hyuk,Lee, Hyun Woong,Kim, Hyung Joon,Song, Mi-Ok,Yoon, Seung-Kew,Park, Jong-Hwa,Chung, In Sik,Kim, Wonyong The Korean Society for Microbiology 2014 Journal of Bacteriology and Virology Vol.44 No.3
Hepatitis A virus (HAV) positive stool samples were collected from acute hepatitis A patients during the two study periods of 2002 and 2011 in Seoul, South Korea, and their genetic variability was determined. From a total of 79 specimens, the nucleotide sequences of the VP1 and 2A junction were successfully amplified in 27 (34.2%) samples and subjected to sequence analysis. Genetically, there was a dramatic change in HAV subgenotypes from IA to IIIA during the past ten years. Sequence analysis identified that most strains belonged to genotype I, which is the main genotype globally. The subgenotype IA (93.3%, n=13/14) was the major subgenotype in 2002, whereas the subgenotype IIIA (69.2%, n=9/13) was predominant in 2011. Interestingly, a IIIA strain was identified from a patient who had a history of travel to India in 2002. The finding presented provides new insight into the genetic shift of circulating HAVs in South Korea.
다관절 해저로봇의 불량 시계환경 극복을 위한 고해상도 주사음탐기의 운용전략과 실험적 고찰
백혁(Hyuk Baek),전봉환(Bong-Huan Jun),김보람(Bo-Ram Kim),심형원(Hyung-Won Shim) 대한조선학회 2012 대한조선학회 학술대회자료집 Vol.2012 No.5
In this paper, we studied about ultrasonic sensor operating strategies of multi-legged seabed robot based on result of the open sea experiment that the robot to overcome the low visibility environment. This experiment was carried out on April 3 to April 6, 2012 at Pier of Namhae Institute to get vertical and horizontal images and used device was 1171 High Resolution sensor manufactured by Kongsberg Maritime. The criteria of positioning and sensor arrangement of the robot was suggested by this experiment and acquired image data was represented as an integrated picture with Google Earth satellite map through the mosaic processing from Adobe Photoshop program.
( In Hyuk Baek ),( Hyung Joon Kim ),( Hyun Woong Lee ),( M Ok Song ),( Thoi Cong Truong ),( Seung Kew Yoon ),( Hee Young Lee ),( Dong Hwa Shon ),( In Sik Chung ),( Wonyong Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: Recently, hepatitis A has become increasingly important because its incidence is dramatically increasing in adolescents and adults in South Korea. HAV infection is now recognized as a public health burden. This situation has resulted in the need for genetic and epidemiological evaluation of hepatitis A virus (HAV) infection. We performed a comparative HAV genetic analysis between non-endemic period and endemic period. Methods: HAV IgM-positive fecal samples were collected from patients with acute hepatitis A at two university hospitals in South Korea during the two study periods of 2002 (n=34) and 2011 (n=45) and their genetic variability was characterized. Results: From a total of 79 specimens, the nucleotide sequences of the VP1 and 2A regions were successfully amplified in 27 (34.2%) samples and subjected to sequence analysis. Phylogenetic analysis showed that most strains belonged to genotype I, which is the major genotype worldwide. The subgenotype IA (93.3%, 13/14) was predominant in 2002, whereas the subgenotype IIIA (69.2%, 9/13) was the major subgenotype in 2011. Notably, a IIIA strain was detected in 2002 from a patient who had a history of travel to India. Conclusions: Genetically, there was a dramatic shift in circulating HAV subgenotypes from IA to IIIA during the past ten years. This report provides new insight into the genetic variation and relatedness of HAV circulating in South Korea.