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( Hyung-seop Youn ),( Tae Gyun Kim ),( Mun-kyoung Kim ),( Gil Bu Kang ),( Jung Youn Kang ),( Jung-gyu Lee ),( Jun Yop An ),( Kyoung Ryoung Park ),( Youngjin Lee ),( Young Jun Im ),( Jun Hyuck Lee ),( 전남대학교 약품개발연구소 2016 약품개발연구지 Vol.25 No.-
Ouinolinate phosphoribosyltransferase (QPRT) catalyses the production of nicotinic acid Mononucleotide, a precursor of de novo biosynthesis of the ubiquitous coenzyme nicotinamide adenine dinucleotide. QPRT is also essential for maintaining the homeostasis of quinolinic acid in the brain, a possible neurptoxin causing various neurodegenerative diseases. Although QPRT has been extensively analysed, the molecular basis of the reaction catalyzed by human QPRT remains unclear. Here, we present the crystal structures of hexameric human QPRT in the apo form and its complexes With reactant or product. We found that the interaction between dimeric subunits was dramatically altered during the reaction process by conformational changes of two flexible loops in the active site at the dimer-dimer interface. In addition, the N-terminal short helix α1 was identified as a critical hexamer stabilizer. The structural features, size distribution, heat aggregation and ITC studies of the full-length enzyme and the enzyme lacking helix α1 stroungly suggest that human QPRT acts as a hexamer for cooperative reactant binding via three dimeric subunits and maintaining stability. Based on our comparison of human QPRT structures in the apo and complex forms, we propose a drug design strategy targeting malignant glioma.
강형우(Hyung-Woo Kang),이수연(Su-Youn Lee),박창섭(Chang-Seop Park),이동훈(Dong-Hoon Lee),윤이중(E-Joong Yoon) 한국정보과학회 1998 한국정보과학회 학술발표논문집 Vol.25 No.2Ⅲ
무선 이동 통신에서는 가입자의 이동성으로 인하여 가입자의 인증 프로토콜을 수행할 때 신원이 노출되기 쉽다. 이러한 가입자의 신원 노출은 제 3자가 이동 가입자의 이동을 추적하거나 위치를 파악할 수 있게 할 수 있다. 또한 이동 가입자가 사용하는 단말기의 낮은 계산능력으로 인하여 가입자의 단말기가 인증 프로토콜을 수행함에 있어서 적은 암호학적 연산이 요구된다. 본 논문에서는 단말기의 계산능력이 낮은 점을 고려하여 단말기에 적은 암호학적 연산이 요구되며 가입자의 위치와 행동의 노출 없이 안전하게 이동 가입자를 인증하는 프로토콜을 제안한다.
증례 : 호흡기 ; 신우신염에 의해 유발된 패혈색전증 1예
이호연 ( Ho Youn Lee ),김남희 ( Nam Hee Kim ),김지환 ( Ji Hwan Kim ),김도형 ( Do Hyung Kim ),김윤섭 ( Yoon Seop Kim ),지영구 ( Young Koo Jee ),박재석 ( Jae Seuk Park ) 대한내과학회 2009 대한내과학회지 Vol.76 No.1
패혈색전증은 다양한 감염성 질환에서 동반될 수 있는 비교적 드문 질환이다. 저자들은 당뇨병 환자에서 Klebsiella pneumoniae에 의한 신우신염의 합병증으로 인해 신정맥 혈전증 및 패혈색전증이 발생하여 항생제 투여로 호전된 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. A septic pulmonary embolism (SPE) is a rare disease originating from septic thrombi from a primary site of infection. Pyelonephritis is a rare cause of inferior vena cava (IVC) thrombosis and SPE. We experienced a case of SPE associated with pyelonephritis, a perirenal abscess, and renal vein and IVC thrombosis in a patient with diabetes mellitus. Prompt antimicrobial therapy led to a successful outcome, with complete resolution of the thrombi. We report a case of SPE due to pyelonephritis with a review of the literature. (Korean J Med 76:105-109,2009)
A549 기도 상피세포에서 GRβ의 과발현이 스테로이드에 의한 전체 유전자 변화에 미치는 영향
이호연 ( Ho Youn Lee ),김남희 ( Nam Hee Kim ),김도형 ( Doh Hyung Kim ),김윤섭 ( Youn Seop Kim ),박재석 ( Jae Seuk Park ),김동훈 ( Dong Hoon Kim ),지영구 ( Young Koo Jee ) 대한천식알레르기학회 2008 천식 및 알레르기 Vol.28 No.3
Background: Glucocorticoid receptor β (GRβ) may not bind to ligands and is thought to affect gene transcription only by acting as a dominant negative inhibitor of Glucocorticoid receptor α (GRα). However, there have been few reports about the functional roles of GRβ in the steroid-induces changes of gene expression. Objective: To investigate the functional role of GRβ overexpression in the steroid-induced changes of gene expression. Method: The changes in gene expressions such as steroid-induced synthesis of genes, TNF-α-induced synthesis of inflammatory genes and steroid induced suppression of activated genes by TNF-α treatment were compared with and without pCMV GRβ expression. Result: The number of genes increased by dexamethasone treatment only in the GRβ transfected group were 54. Increased expressions of genes by dexamethasone treatment were not affected by GRβ overexpression. The number genes increased by TNF-α treatment only in the GRβ-transfected group were 69. Expressions of genes increased by TNF-α treatment in both the control and the GRβ-transfected group were decreased by further dexamethasone treatment in a similar manner. Conclusion: The results of this study suggest that GRβ may not be a dominant negative inhibitor in the steroid-induced changes of genes and that it may have some functional roles in the regulation of gene expressions. (Korean J Asthma Allergy Clin Immunol 2008;28: 220-225)
Lee, Jung-Gyu,Youn, Hyung-Seop,Kang, Jung Youn,Park, Sam-Yong,Kidera, Akinori,Yoo, Yung Joon,Eom, Soo Hyun Elsevier 2018 Biochemical and biophysical research communication Vol.506 No.1
<P><B>Abstract</B></P> <P>Ubiquitin-conjugating enzymes (E2) form thioester bonds with ubiquitin (Ub), which are subsequently transferred to target proteins for cellular progress. Ube2K/E2-25K (a class II E2 enzyme) contains a C-terminal ubiquitin-associated (UBA) domain that has been suggested to control ubiquitin recognition, dimerization, or poly-ubiquitin chain formation. Ube2K is a special E2 because it synthesizes K48-linked poly-ubiquitin chains without E3 ubiquitin ligase. We found that a novel interaction between the acceptor di-Ub (Ub<SUB>2</SUB>) and the auxiliary Ube2K promotes the discharging reaction and production of tri-Ub (Ub<SUB>3</SUB>), probably by guiding and positioning the K48 (in the distal Ub) of the acceptor Ub<SUB>2</SUB> in the active site. We also determined the crystal structure of Ube2K-Ub<SUB>2</SUB> at 2.47 Å resolution. Based on our structural and biochemical data, we proposed a structural model of Ub<SUB>3</SUB> synthesis by Ube2K without E3.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The crystal structure of the Ube2K-Ub<SUB>2</SUB> complex was determined at a 2.47 Å resolution. </LI> <LI> A novel interaction between the acceptor Ub<SUB>2</SUB> and the auxiliary Ube2K was observed. </LI> <LI> Auxiliary Ube2K promotes the discharging reaction and production of Ub<SUB>3</SUB>. </LI> <LI> We propose a structural model of the Ub<SUB>3</SUB> synthesis by Ube2K. </LI> </UL> </P>
Long-term clinical study and multiscale analysis of in vivo biodegradation mechanism of Mg alloy
Lee, Jee-Wook,Han, Hyung-Seop,Han, Kyeong-Jin,Park, Jimin,Jeon, Hojeong,Ok, Myoung-Ryul,Seok, Hyun-Kwang,Ahn, Jae-Pyoung,Lee, Kyung Eun,Lee, Dong-Ho,Yang, Seok-Jo,Cho, Sung-Youn,Cha, Pil-Ryung,Kwon, H National Academy of Sciences 2016 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.113 No.3
<P>There has been a tremendous amount of research in the past decade to optimize the mechanical properties and degradation behavior of the biodegradable Mg alloy for orthopedic implant. Despite the feasibility of degrading implant, the lack of fundamental understanding about biocompatibility and underlying bone formation mechanism is currently limiting the use in clinical applications. Herein, we report the result of long-term clinical study and systematic investigation of bone formation mechanism of the biodegradable Mg-5wt% Ca-1wt% Zn alloy implant through simultaneous observation of changes in element composition and crystallinity within degrading interface at hierarchical levels. Controlled degradation of Mg-5wt% Ca-1wt% Zn alloy results in the formation of biomimicking calcification matrix at the degrading interface to initiate the bone formation process. This process facilitates early bone healing and allows the complete replacement of biodegradable Mg implant by the new bone within 1 y of implantation, as demonstrated in 53 cases of successful long-term clinical study.</P>
A549 기도상피세포에서 IL-8 전사와 분비에 미치는 Dexamethasone과 Troglitazone의 병합투여효과
김남희 ( Nam Hee Kim ),이호연 ( Ho Youn Lee ),서지현 ( Ji Hyun Suh ),한성환 ( Sung Hwahn Hahn ),김도형 ( Doh Hyung Kim ),김윤섭 ( Youn Seop Kim ),박재석 ( Jae Seuk Park ),지영구 ( Young Koo Jee ) 대한천식알레르기학회 2008 천식 및 알레르기 Vol.28 No.4
Background: Glucocorticoid is a widely used anti-inflammatory agent in a variety of diseases. Recently, peroxisome proliferator activated receptor γ (PPARγ) ligand has been shown to exert a potential anti-inflammatory activity, mainly through their ability to down- regulate pro-inflammatory gene expressions. Objective This study was designed to evaluate the effects of co-treatment with dexamethasone and troglitazone (a ligand of PPARγ) on IL-8 transcription and secretion. Method: Steroid and troglitazone-induced transactivation was measured by using pGRE luciferase reporter gene in the A549 cell line. Steroid-induced transrepression was measured by using stable A549 IgG-NFκB luciferase cell line, which contained the minimal IL-8 promoter region. Result: Co-treatment with dexamethasone and troglitazone showed no additional increase of transactivation activity compared to dexamethasone single treatment. Suppressions of the transcriptional activity in the IL-8 promotor and IL-8 secretion showed no difference in the co-treatment with dexamethasone and troglitazone compared to those in dexamethasone single treatment. Conclusion: Our results suggest that co-treatment with PPARγ ligand and steroid may not have an additive anti-inflammatory activity at least in the airway epithelial cell. (Korean J Asthma Allergy Clin Immunol 2008;28:292-297)