프롤린 함량 증가 및 내건성 강화 관련 새로운 RING-Type E3 유비퀴틴 리가아제 애기장대 atrzf1 돌연변이체의 생리적 기능 연구

주현우,민지희,정문수,김철수 전남대학교 농업과학기술연구소 2021 농업생명과학연구동향 Vol.59 No.-

The covalent attachment of ubiquitin to proteins plays a fundamental role in the regulation of cellular function through biological events involving abiotic of biotic stress responses, immune responses, and apoptosis, Here, we characterize the biological function of the Arabidopsis thaliana RING Zinc Finger 1 (AtRZF1) in dehydration response. AtRZF1 was significantly reduced by drought stress. The atrzf1 mutant was less sensitive to osmotic stress than the wild-type during early seedling development, wheareas transgenic pants overexpressing AtRZF1 were hypersensitive, indicating that AtRZF1 megatively regulates drought-mediated control of early seedling developmeng. Moreover, the ectopic expression of the AtRZF1 gene was very significantly influential in drought sensitiv parameters including proline content, water loss, membrance ion leakage and the expression of dehy-dration stress-relat genes. ARZF1 is a functional E3 ubiquitin ligase, and its conserved C3H2C3-type RING domain is likely important for the biological function of AtRZF1 in drought response. Together, these results suggest that the E3 ligase AtRZF1 is an important regulator of water deficit stress during early seedling development.

Sr·페라이트 플라스틱자석의 제조에 관한 연구

문현욱,최희태,서강수,신용진 明知大學校 産業技術硏究所 1995 産業技術硏究所論文集 Vol.14 No.-

In this paper, we mention he fabrication of Sr ferrite plastic magnet. In order to compose Sr ferrite, first, a little CaO, SiO₂, Na₂SiO₃, and Al₂O₃ are added to SrO5.9Fe₂O₃. Then, the composition is calcinated at 1225℃ under N₂ enviroment for 1 hourwith addition of PVA 5wt% solution. The carbon coating method increase grinding effect on powder fabrication. To fabricate anisotropic Sr ferrite plastic magnet, 80-89wt% of Sr ferrite and polyamide6 are kneaded and then 0-12kOe of magnetic field is applied. On magnet fabrication, an optimal condition of injection mold is obtained under the injection temperature 270℃, the injection pressure 120㎏/㎠, the magnetic field 8kOe and magnetic field applied time 8sec. As the result of experiments, we obtain (BH)max = 2.09MGOe property on anisotropic plastic magnet which is fabricated by using injection mold method in magnetic field.

잡음환경에서의 음성인식

문현택,이병수 순천향대학교 부설 산업기술연구소 1997 순천향 산업기술연구소논문집 Vol.3 No.2

In this thesis, problems connected with present speech recognition systems are pointed out and methods for performance improvement are proposed. Especially, a recognition system for use in a car noise environment is proposed and estimated. A speech recognition system comprises feature extraction, distance measure and pattern classification methods. Recently, weighted cepstral distance measures using LPC-cepstral coefficients as feature vectors have given high recognition rates, with HMM and DTW are widely used for pattern classification. But speech recognition rates drop rapidly in a noisy environment. To improve recognition accuracy in noise, an endpoint detection method which takes noise into account, a modified DTW, an autocorrelation subtraction method and a distance measure robust to noise are used to give improved speaker dependent isolated word recognition performance.

카아네이션 잎마름병 (Alternaria dianthi Stevens & Hall)의 발생에 관하여

文炳周,孫賢秀,金正基 東亞大學校 大學院 1984 大學院論文集 Vol.8 No.2

The study has been carried to examine a new disease of carnation caused by Alternaria isolate, which was found in Gim Hae, Gyoungnam province and the suburbs of Busan City, recently. 1. The pathogen was identified as Alternaria dianthi Stevens & Hall which has not been described in Korea. The common name of the disease was given as IB Ma Reum Byung(carnation blight). 2. Morphological studies of the fungus have shown a wide variation in shape, size and septation of conidia. The size of conidia formed on carnation leaf lesions in field is 22.8-101.2μ(av. 60.4μ)×10.1-27.8μ(av. 18.6μ), which was larger than those on potato dextrose agar in vitro. 3. Of 13 carnation varieties tested, Boston, Linda and Red Alaska were highly susceptible, and Angel, Flamingo Sim, William Sim and Yo Soo were moderately susceptible whereas Brauns Yollw Sim, Colquate, Imp Sisney Little Field, Cardinal Sim, La Reve Salmon Sim and Safari were slightly susceptible to the fungus under the condition of artificial infection.

효율적인 분할 시그너춰 화일의 디렉토리 관리

문현수,황환규,최황규,염상민,김상욱 강원대학교 정보통신연구소 1998 정보통신논문지 Vol.2 No.-

A partitioned signature file is an enhancement of the signature file that divides all the signatures into blocks in such a way that each block contains the signatures with the same key. Its directory stores all the keys as meta information for avoiding unnecessary block accesses by examining them first before the actual searching of the blocks. Efficient directory management is very important in large database environments since its size gets larger proportionally to that of the database. In this paper, we first point out the problems in the directory management methods of the previous partitioned signature files, and then pressent a new one solving them. Our method offers good features in the following three aspects: (1) suitability for large database environments, (2)adaptability to dynamic situations, and (3) storage overhead for the directory.

過蓋咬合, 開咬合 및 正常咬合의 咬筋沈默期에 關한 硏究

문철현,정현수 대한치과교정학회 1987 대한치과교정학회지 Vol.17 No.1

The present study was carried out to investigate the relationships between the depth of overbite and the masseteric silent period. Normal subjects of 44 were selected, which consisted of 9 open bites, 24 normal overbites and 11 deep bites, all were 19-29 years of age. EMG activity was recorded on the bilateral masseteric muscles and craniofacial radiography was done. The following results were obtained. 1. The mean duration of masseteric silent period was 18.58±4.50 msec in open bite, 17.37±7.05 msec in normal overbite and 19.30±7.62 msec in deep bite groups. 2. There were no significant differences on masseteric silent period among open bite, normal overbite and deep bite groups. 3. There were no significant correlations between masseteric silent period and craniofacial variables.

재조합 Saccharomyces cerevisiae로부터 인체 리포코틴-Ⅰ의 분비 생산 및 정제

김병문,정봉현,이상기,박영훈,남수완 동의대학교 기초과학연구소 1996 基礎科學硏究論文集 Vol.6 No.1

Human lipocortin-I (LCI) is a calcium ion-dependent and phospholipid-binding protein which exhibits an anti-inflammatory activity by inhibiting phospholipase A₂ activity. In this study, the LCI gene containing its own terminator region was joined to GAL10 promoter-ppL (prepro-leader sequence of mating factor a). An ATG start codon of LCI gene was placed at downstream with KR endoprotease recognition site (Lys-Arg) of ppL. Recombinant S. cerevisiae harboring the LCI expression/secretion vector, pYGLPT5, was aerobically grown on a liquid YPDG medium at 30℃ for 72hrs. The whole cell and culture supernatant were separated after centrifugation, and the expressed LCI was analyzed by SDS-PAGE and western blotting methods. A majority fraction of the expressed LCI was found to be accumulated in the intracellular fraction, resulting in very low secretion efficiency of about 7.4%. About 500mg/ℓof LCI was extracellularly produced by the fed-batch culture employing the controlled-feeding of glucose and galactose. The secreted LCI was purified by ultrafiltration and hydroxylapatite column chromatography, and a purity of more than 99% was obtained.

PC에 의한 3차원 형상 모델링 연구(Ⅰ)

변문현,오익수 忠南大學校 産業技術硏究所 1988 산업기술연구논문집 Vol.3 No.2

The purpose of this study is to develop a personal computer aided geometric modeller. To perform this study, we set up a cube, a cylinder, and a prism as primitives in the first segment of this study. By modelling the 3-D object through their transformation, and addition, we proved the validity of the developed algorithm and its computer program. Some examples show the results of applying the program to model a few simple shape of the machine parts. These results met the first aim of this study.

클러스터링 기법을 이용한 퍼지 모델링에 관한 연구

원태현,김문수,이용길,강석규 동의공업대학 2000 論文集 Vol.26 No.1

In this paper, a new approach to modeling of static and dynamic systems using clustering techniques. To express the various and complex behavior of systems, we combine multiple model method. The Gustafson-Kessel algorithm is used in partitioning of system, and Takaei Sugeno rule structure is adopted to form the fuzzy rules. According to simulation, the performance of fuzzy modeling technique is evaulated.

NURBS 곡면과 경계표현에 의한 솔리드모델의 결합에 관한 연구

변문현,오익수 忠南大學校 産業技術硏究所 1994 산업기술연구논문집 Vol.9 No.2

Geometric modeling system is a fundamental module in the CAD/CAM system. Solid medeling has been usually used in analytical geometric shape modeling and surface modeling is very useful to represent a free form surface. But the most of industrial products consisit of compound surfaces that are formed with free form surface and analytical surface. Therefore, solid modeling system should support to form a compound surface. In this study, a solid model and a NURBS surface are combined to represent a compound surface and all the compound surfaces are represented by halfedge data structure.