운동 직후 고온 침수가 EPOC와 TG/ fatty acid cycling에 미치는 영향

조현철,김종규,강민철,홍완표,박노혁 龍仁大學校 體育科學硏究所 2005 體育科學硏究論叢 Vol.15 No.1

The purpose this study was to estimate effects of EPOC and TG/fatty acid cycling on warm water immersion of immediately after exercise. To elucidate the role of fatty metabolism, a sequence of five experiments was performed. Seven physically active, male subjects volunteered to participate in the presented study. The mean values for age, body mass and hight were 25±1.52 yr, 79.2±9.52kg, 177.2±4.62cm, respectively. After giving consent, participant visited the laboratory on six occasion: 1) 30min of treadmill exercise VO2max 55% and a further 60min recovery, 2) 30min partial body warm water immersion in a 39℃ and a further 60min recovery, 3) 30min whole body warm water immersion in a 39℃ and a further 60min recovery, 4) 30min of treadmill exercise VO2mas 55% and in 30min partial body warm water immersion in a 39℃, 5) 30min of treadmill exercise VO2max 55% and in 30min whole body warm water immersion in a 39℃. When compared by recover period within repeat on the base of changes of subjects average body temperature, not effective interactions among repeat. However, partial and whole body warm water immersion immediately after exercise shows it as the best effective exercise for VO2max 55%, partial and whole body warm water immersion. When compared by recover period within repeat on the base of changes of subjects EPOC, effective interactions among repeat(p<.05). Partial and whole body warm water immersion immediately after exercise shows it as the best effective exercise for VO2mas 55%, partial and whole body warm water immersion. The catecholamines concentration was significantly higher partial and whole body warm water immersion than exercise of VO2max 55%(p<.05). The TG concentration and free fatty acid was significantly higher partial and whole body warm water immersion immediately after exercise than exercise of VO2max 55% than Partial and whole body warm water immersion(p<.05). Based on the facts that we have discussed above, human metabolism is increased by both exercise and conditions of immersion and partial and whole body warm water immersion immediately after exercise than exercise of VO2maw 55% shows it as better effective treatment for increasing TG/Fatty acid cycling activation. Due to extremely heavy stress complained by subjects during whole body immersion, it is thought that more researches on it should be required.

실험적 급성 허혈성 신부전에서 Verapamil 이 혈중 Polyamine 동태에 미치는 영향

원동준(Dong Jun Won),권현민(Hyun Min Kwon),김용섭(Yong Seop Kim),구자룡(Ja Ryong Gu),권영주(Young Ju Kwon),조원용(Won Yong Cho),김형규(Hyung Kyu Kim) 대한내과학회 1991 대한내과학회지 Vol.40 No.6

N/A It has been proposd that calcium entry from an external medium increases intracellular free calcium to toxic levels during ischemic acute renal failure, and verapamil (ARF) has been suggested as the agentblocking calicium entry into renal cells and protecting renal function during ischemic injury. Polyamines, anorganic cations that play various roles in normal cellular proliferation and differentiation, accumulate in renal failare. Alsa it has been suggested tht the synthesis and metabolism of polyamine are influence by acute changes of the general condition, such as acute renal failure, and are mediated by a calcium influx into the cells. The study was designed to study the protective effects of systemic verapamil pretreatment on renal function and the influence on polyamine metabolism in experimental ischemic ARF in cats. For these purpose, the experimental animals were divided into 2 groups. While Group I (n=5) was an ischemic ARF model by renal artery clamping for 60 minutes, Group II (n=5) was ischemic ARF with systemic verapamil (5 ml/min/kg) pretreatment. The creatinine clearance and plasma and urinary polyamine were measured in each group before and after the renal artery clamp. The results were as follows: 1) Creatinine clearance before and after the renal artery clamp were 10.64±7.18 ml/min/kg and 2.09±1. 70 ml/min/kg in Group I, 4.47±3.38 ml min/kg and 0.60±0.79ml/min/kg in Group II, respectively, So creatinine clearance decreased more significantly in Group II campared with Group I. 2) Plasma polyamine increased after ischemia in Group I. In group I, plasma levels of putrescine, spermidine, and spermine before ischemia were 4.75±0.40 nmol/ml, 0.69±0.09 nmol/ml, and 0.83±0.63 nmol/ml, were elevated to 7.17±2.91 nmol/ml, 9.83±1.46 nmol/ ml, and 2.64±1.14nmol/ml after ischemia. But in Group II, the plasma level of polyamine was not changed, and especially, spermine decreased significantly from 0.83±0.27 before ischemia to 0.49±0.23 nmol/ml after ischenmine (p=0.033). 3) Urine polyamine excretion decreased after ischemia in Group I and Group II. In Group II, urinary excretion of spermidine and spermine before ischemia, 0.13±0.10 nmol/min and 0.17±0.13nmol/min, decreased after ischemia to 0.01±0.01nmol/min (p=0.019) and 0.032±0.26 nmol/min (p=0.0257). 4) In renal tissue, spermine content vas highest. In Group II, preischemic spermine were 397.20 nmol/g and increased to 646.66nmol/g after ischema, But there were no significant changes in the polyamine contents in Group II. From these data, it was suggested that systemic verapamil pretreatment exerts no protective effect on ischemic ARF. Plasma polyamines are elevated in ischemic ARF, and verapamil may protect these elevations.

Robot assisted laparoscopic myomectomy, an alternative to laparotomy for numerous myomas (over 10)

( Hyun-kyung Kim ),( Min-kyung Lee ),( Yoon-jee Cheung ),( Hyun-hee Cho ),( Jang-heub Kim ),( Mee-ran Kim ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: To evaluate the feasibility of robot-assisted laparoscopic myomectomy in multiple myomas over 10 in number and to compare outcomes with open myomectomy. 방법: This study is a retrospective chart review of 216 patients who underwent robot assisted laparoscopic myomectomy by a single operator at St. Mary's Fibroid Center in Seoul between October 1, 2010 and January 31, 2014; in the selected patients, 10 or more uterine myomas were removed. We reported the characteristics of the removed myomas including the with maximum diameter of the myomas, the sum of the diameter of each myoma, and the types of the combined surgeries. we organized surgical outcomes that were measured including the operative time, estimated intraoperative blood loss, length of hospital stay, and perioperative complications. And we compared these surgical outcomes with cases of open myomectomy. 결과: A total of 13 women underwent the removal of 10 or more uterine myomas by robotics. The patient age was 37.6±3.6 years, and all of the patients were nulliparous. The operation time was 389.8±100.6 min. The number of removed myomas for each case was 13.1±3.8 (range 10-20). The myoma with the maximum diameter was 6.5±0.7 centimeters (range 5.0-8.0 centimeters). The sum of the diameters was 34.9 ±9.6 centimeters (range 20.0-54.5 centimeter. The Estimated blood loss during operation was 219.2±158 milliliter. . The postoperative hospital stay was 2.6±0.6 days. Compared to laparotomic adenomyomectomy, robotic surgery has strengths that enables less blood loss, shorter hospital stay and decreasing risk of blood transfusion although it takes more operation time. 결론: This report is the first to describe the feasibility of robot-assisted laparoscopic myomectomy in multiple myomas over 10 in number. In robot- assisted laparoscopic myomectomies, supplementary techniques including preoperative MRI assessment, intraoperative sonographic navigation, and palpation by an assistant to localize the myomas are helpful in overcoming the absence of haptic perception. This technique is considered to be an alternative to laparotomy for more than 10 myomas.

Analysis of Lignans in Acanthopanax sessiliflorus Fruits and Their Fermented Wine by HPLC

Hye-Min Kim,Ju-Sun Kim,Seon-Haeng Cho,Sam-Sik Kang,Dae-Sung Cheoi,Sang-Hyun Lee 한국약용작물학회 2006 한국약용작물학회지 Vol.14 No.5

High performance liquid chromatography (HPLC) was used for the determination of lignans, eleutherosides B and E, in Acanthopanax sessiliflorus fruits and their fermented wine. The lignans were quantified by a reversed-phase system using a gradient of H2O and acetonitrile as a mobile phase within 20 min. The analysis was successfully carried out within 20 min. The contents of eleutherosides Band E as main active principles of Acanthopanax species were measured in A. sessiliflorus fruits (1.15 and 8.49 μg/mg, respectively), their fermented wine (0.45 and 1.33 μg/mg, respectively) and wine residues (no detection).

Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

반응표면분석법을 이용한 배 퓌레 열수처리 조건 최적화

박민경(Min Kyeong Park),조용식(Yong Sik Cho),장현욱(Hyun Wook Jang) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.11

본 연구는 열수처리 온도(50, 70, 90°C) 및 열수처리 시간(15, 30, 45분)을 달리하여 배 퓌레를 제조한 후 품질특성을 분석하여 반응표면분석법을 통해 최적의 열수처리 조건을 구하고자 하였고, 중심합성계획법에 따라 총 9가지의 실험점에서 진행하였다. 열수처리 온도 및 시간을 독립변수로 정하고, 품질특성에 관련된 적색도, ΔE값, 일반세균, 대장균군, polyphenol oxidase 활성, 총 폴리페놀 함량, 총 플라보노이드 함량을 종속변수로 설정하였다. 독립변수가 종속변수에 미치는 영향에 대해 분석한 결과, 적색도와 갈변을 나타내는 ΔE값은 각각 -0.38~1.20, 0.97~11.88의 범위로 측정되었고, 반응식의 R2은 각각 0.9754, 0.9818이었다. 일반세균 및 대장균군의 생균수는 각각 0.00~4.91 log CFU/mL, 0.00~4.03 log CFU/mL의 범위였고, 반응식의 R²은 0.9849, 0.9714였다. 갈변의 원인이 되는 polyphenol oxidase 활성은 122.57~235.13 unit 범위였으며, 반응식의 R²은 0.8616의 값을 보였다. 배 퓌레의 유효성분으로 총 폴리페놀 함량 및 총 플라보노이드 함량은 각각 19.33~24.54 GAE mg/100 g, 4.56~5.78 GAE mg/100 g 범위였으며, 이때 반응식의 R²은 각각 0.9181, 0.9097을 나타내었다. 이를 바탕으로 contour map을 superimposing 하여 최적 열수처리 조건을 설정한 결과, 최적화된 열수처리 조건의 온도범위는 69.3~80.4°C, 시간 범위는 16.8~25.8분으로 나타났으며, 최적점은 74.6°C, 20.7분으로 나타났다. 이에 대해 모델의 신뢰성을 검증하기 위해 예측값과 실험값을 비교한 결과, 각 반응변수의 실험값은 모두 예측값의 95% 신뢰구간 및 예측구간 범위에 들어와 모델의 적합성을 확인하였다. This study analyzed the quality characteristics of pear puree by varying the hydrothermal treatment temperature (50, 70, and 90°C) and time (15, 30, and 45 min) and sought to determine the optimal hydrothermal treatment conditions through the response surface methodology. A total of nine experimental points were studied according to the central composite design. The temperature and time were set as independent variables, and the redness, ΔE value, aerobic bacteria, coliform group, polyphenol oxidase (PPO) activity, total polyphenol content (TPC), and total flavonoid content (TFC) related to the quality characteristics were set as dependent variables. The redness and the ΔE value were measured as −0.38∼1.20 and 0.97∼11.88, respectively, and the R² value was 0.9754 and 0.9818, respectively. The R2 value of viable cell counts were 0.9849 and 0.9714 in the aerobic bacteria and coliform groups. The PPO activity was 122.57∼235.13 g/min, and the R² value was 0.8616. The TPC and TFC as active ingredients of pear puree were in the range of 19.33∼24.54 and 4.56∼5.78 GAE mg/100 g, respectively, and the R² values were 0.9181 and 0.9097, respectively. Based on this, the contour map was superimposed to set the optimal hydrothermal treatment conditions. The optimum points derived were 74.6°C and 20.7 min. When the predicted and the experimental values were compared to verify the reliability of the model, all the experimental values of each of the response variables were within the 95% confidence and prediction intervals, confirming the suitability of the model.

3D Asthenopia in Horizontal Deviation

Kim, Seung-Hyun,Suh, Young-Woo,Yun, Cheol-Min,Yoo, Eun-Joo,Yeom, Ji-Hyun,Cho, Yoonae A. Informa Healthcare USA, Inc. 2013 Current eye research Vol.38 No.5

<P><I>Purpose:</I> This study was conducted to investigate the asthenopic symptoms in patients with exotropia and esotropia while watching stereoscopic 3D (S3D) television (TV).</P><P><I>Methods:</I> A total 77 subjects who more than 9 years of age were enrolled in this study. We divided them into three groups; Thirty-four patients with exodeviation (Exo group), 11 patients with esodeviation (Eso group) and 32 volunteers with normal binocular vision (control group). The S3D images were shown to all patients with S3D high-definition TV for a period of 20 min. Best corrected visual acuity, refractive errors, angle of strabismus, stereopsis test and history of strabismus surgery, were evaluated. After watching S3D TV for 20 min, a survey of subjective symptoms was conducted with a questionnaire to evaluate the degree of S3D perception and asthenopic symptoms such as headache, dizziness and ocular fatigue while watching 3D TV.</P><P><I>Results:</I> The mean amounts of deviation in the Exo group and Eso group were 11.2 PD and 7.73PD, respectively. Mean stereoacuity was 102.7 arc sec in the the Exo group and 1389.1 arc sec in the Eso group. In the control group, it was 41.9 arc sec. Twenty-nine patients in the Exo group showed excellent stereopsis (≤60 arc sec at near), but all 11 subjects of the Eso group showed 140 arc sec or worse and showed more decreased 3D perception than the Exo and the control group (<I>p</I> < 0.001, Kruskal-Wallis test). The Exo group reported more eye fatigue (<I>p</I> < 0.001, Kruskal-Wallis test) than the Eso and the control group. However, the scores of ocular fatigue in the patients who had undergone corrective surgery were less than in the patients who had not in the Exo group (<I>p</I> < 0.001, Kruskal-Wallis test) and the amount of exodeviation was not correlated with the asthenopic symptoms (dizziness, <I>r</I> = 0.034, <I>p</I> = 0.33; headache, <I>r</I> = 0.320, <I>p</I> = 0.119; eye fatigue, <I>r</I> = 0.135, <I>p</I> = 0.519, Spearman rank correlation test, respectively).</P><P><I>Conclusion:</I> Symptoms of 3D asthenopia were related to the presence of exodeviation but not to esodeviation. This may indicate that S3D symptoms are closely related to the convergence demand.</P>

Analysis of Lignans in Acanthopanax sessiliflorus Fruits and Their Fermented Wine by HPLC

Kim, Hye-Min,Kim, Ju-Sun,Cho, Seon-Haeng,Kang, Sam-Sik,Cheoi, Dae-Sung,Lee, Sang-Hyun The Korean Society of Medicinal Crop Science 2006 韓國藥用作物學會誌 Vol.14 No.5

High performance liquid chromatography (HPLC) was used for the determination of lignans, eleutherosides B and E, in Acanthopanax sessiliflorus fruits and their fermented wine. The lignans were quantified by a reversed-phase system using a gradient of $H_2O$ and acetonitrile as a mobile phase within 20 min. The analysis was successfully carried out within 20 min. The contents of eleutherosides Band E as main active principles of Acanthopanax species were measured in A. sessiliflorus fruits (1.15 and $8.49\;{\mu}g/mg$, respectively), their fermented wine (0.45 and $1.33\;{\mu}g/mg$, respectively) and wine residues (no detection).

폐 젤리충진 통신케이블로부터 고순도 구리회수를 위한 대형화 방안 연구

조성수 ( Sung Su Cho ),이수영 ( Soo Young Lee ),서민혜 ( Min Hye Seo ),엄성현 ( Sung Hyun Uhm ) 한국공업화학회 2014 공업화학 Vol.25 No.2

폐 젤리충진 통신케이블로부터 고순도 구리회수를 위하여 실리콘계 및 톨루엔계 합성 열매유와 폐식용유를 사용하여 적용성을 평가하였다. 실리콘계 오일의 경우 모든 조건에서 분리효율이 낮은 반면, 톨루엔계 오일 및 폐식용유의 경우 처리온도 및 시간을 조절함에 따라 높은 분리효율을 얻을 수 있었다. 폐식용유를 사용하는 50 kg급 밀폐형 대용량 설비를 이용하여 300 ℃에서 60 min 이상 처리할 경우 99.2% 이상의 고순도 구리와이어를 100% 회수할 수 있었다. We examined and compared the feasibility of vegetable oils with synthetic thermal conductive oils to recover highly purified copper metal from waste jelly-filled communication cables. While polydimethylsiloxane shows relatively poor separation effi-ciency under entire operating conditions, dibenzyltoluene and waste vegetable oil show the high separation efficiency if the appropriate operating temperature and time were given. By running 50 kg-class equipment with waste vegetable oils, we ob-tained 100% copper metal recovery with 99.2% purity at 300 ℃ for 60 min.

청소년들의 최대 골량 향상을 위한 운동 가이드라인

민석기 ( Seok-ki Min ),오태웅 ( Taewoong Oh ),김상현 ( Sang Hyun Kim ),조진경 ( Jinkyung Cho ),정호연 ( Ho-yeon Chung ),박동호 ( Dong-ho Park ),김창선 ( Changsun Kim6 ) 한국운동생리학회(구 한국운동과학회) 2020 운동과학 Vol.29 No.1

PURPOSE: An increase in bone mineral density during adolescence increases resistance to fractures in older age. The Korean Society for Bone and Mineral Research and the Korean Society of Exercise Physiology developed exercise guidelines to increase the peak bone mass (PBM) in adolescents based on evidence through a systematic review of previous research. METHODS: Articles were selected using the systematic method, and the exercise guidelines were established by selecting key questions (KQs) and defining the effects of exercises based on evidence through a literature review for selecting the final exercise method. There were 9 KQs. An online search was conducted on articles published since 2000, and 93 articles were identified. RESULTS: An increase in PBM in adolescence was effective for preventing osteoporosis and fractures in older age. Exercise programs as part of vigorous physical activity (VPA) including resistance and impact exercise at least 5 to 6 months were effective for improving PBM in adolescents. It is recommended that resistance exercise is performed 10 to 12 rep·set^-1 1-2 set·region^-1 and 3 days·week^-1 using the large muscles. For impact exercises such as jumping, it is recommended that the exercise is performed at least 50 jumps·min^-1, 10 min·day^-1, and 2 days·week^-1. CONCLUSIONS: Exercise guidelines were successfully developed, and they recommend at least 5 to 6 months of VPA, which includes both resistance and impact exercises. With the development of exercise guidelines, the incidence of osteoporosis and fractures in the aging society can be reduced in the future, thus contributing to improved public health.