A selective cyclin-dependent kinase 4, 6 dual inhibitor, Ribociclib (LEE011) inhibits cell proliferation and induces apoptosis in aggressive thyroid cancer

Lee, Hyun Joo,Lee, Woo Kyung,Kang, Chan Woo,Ku, Cheol Ryong,Cho, Yoon Hee,Lee, Eun Jig Elsevier 2018 Cancer letters Vol.417 No.-

<P><B>Abstract</B></P> <P>The RB-E2F1 pathway is an important mechanism of cell-cycle control, and deregulation of this pathway is one of the key factors contributing to tumorigenesis. Cyclin-dependent kinases (CDKs) and Cyclin D have been known to increase in aggressive thyroid cancer. However, there has been no study to investigate effects of a selective CDK 4/6 inhibitor, Ribociclib (LEE011), in thyroid cancer. Performing Western blotting, we found that RB phosphorylation and the expression of Cyclin D are significantly higher in papillary thyroid cancer (PTC) cell lines as well as anaplastic thyroid cancer (ATC) cell lines, compared with normal thyroid cell line and follicular thyroid cancer cell line. LEE011 dose-dependently inhibited RB phosphorylation and also decreased the expressions of its target genes such as <I>FOXM1, Cyclin A1,</I> and <I>Myc</I> in ATC. Furthermore, LEE011 induced cell cycle arrest in G0-G1 phase and cell apoptosis, and inhibited cell proliferation in ATC. Consistently, oral administration of LEE011 to ATC xenograft models strongly inhibited tumor growth with decreased expressions of pRB, pAKT and Ki-67, and also significantly increased tumor cell apoptosis. Taken together, our data support the rationale for clinical development of the CDK4/6 inhibitor as a therapy for patients with aggressive thyroid cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> pRB and Cyclin D were expressed high in aggressive thyroid cancer. </LI> <LI> LEE011 suppressed pRB and also decreased the expressions of its target genes in ATC. </LI> <LI> LEE011 induced cell cycle G1 arrest and apoptosis, and inhibited cell proliferation. </LI> <LI> LEE011 inhibited in vivo tumor growth with decreased expressions of pRB and Ki-67. </LI> <LI> We could explain the anticancer effects with the RB-E2F pathway. </LI> </UL> </P>

해방 후 李承燁의 ‘統一民族國家’ 건설운동(1945~1953)

이현주(Lee, Hyun-Joo) 인천대학교 인천학연구원 2007 인천학연구 Vol.6 No.-

It was through the process of a merger of three parties that Lee Seung-Yeop rose as a leader of the socialist state movement in South Korea. The inauguration ceremony of the South Korean Worker's Party (남조선노동당) was held under his leadership in November, 1946 after Park Hun-young went to North Korea. He visited secretly PeyongYang accompanied by Kim Sam-yong and achieved the principal that the Opposing Parties individually had to join the South Korean Worker's Party established by centralizing the Korean Communist party. After crossing over the border into North Korea, Lee Seung-Yeop was appointed as a head of judicature of the First Cabinet, North Korea. However, his role was prominent in the Parties. Lee Seung-Yeop rose to the position supervising operations against the South as second secretary of the Party as well as was elected an member of Nine Politics Committeemen of the Korean Workers' Party(조선노동당) which was born due to the merger of Parties. Since then, his activities were concentrated on the leadership for guerrilla war by a partisan. He reorganized a guerrilla unit into three groups in order to develop systematically the guerrilla that was being sporadically progressed in South Korea in July, 1947 and the first group was dispatched to the South in his order. The Korean War was a watershed deciding his fate. He who sent to the South as plenipotentiary of liberated area as soon as the war occurred suddenly was nominated a representative of Seoul city of the Armed Services Committee, North Korea and Seoul temporary Council of People's Commissars the minute that Seoul was occupied. He, an chairman of Council People's Commissars, had to support the People's Army advancing South, recovering ruined product facilities, roads, bridges etc. The management for relief programs, citizens' mobilization, letting volunteer armies go to the war, cultural propaganda programs, land reform, health movement, product power increase was encouraged for about two months. Orders for a guerilla continued. At the same time, Lee Seung-Yeop made people sympathize with the People's Army by infiltrating espionage operators each province, landing the mustered guerrilla to the South through the East Sea on June 25, 1950. Even though his power decreased in the situation an armistice was argued, the first half of 1951, his leadership to the guerrilla was well. The end of the war with Incompletion brought a tragedy to him. Lee Seung-Yeop was arrested with other 11 fellows of the South Korean Worker's Party faction on August 3, 1952. He disappeared in the North, 'a forever strange land', after being sentenced to death and property confiscation upon a quick trial for 4 days, on August from 3 to 6, 1953. The event was not ended in Lee's death. The Purge against the South Korean Worker's Party continued until 1955 and became an overture of the purge against Soviet Union Group(소련파) and Yeon Ahn Group(연안파). His tragic death was not resulted from 'conspiracy of armament riots for the plot against government', but the fact he couldn't clean himself of suspicion even in North Korea, not Kim Il-Sung but Pak Hung-Young whom he assisted as the chief until he died, even if shouting many times 'General Kim Il Sung cheers!' at the official meeting. He failed to free himself from 'a second man of the South Korean Worker's Party until the last moment.

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

Periperformativity and Ethnicity in the Theater of Young Jean Lee

Hyun Joo Lee 한국아메리카학회 2018 美國學論集 Vol.50 No.2

This essay offers a detailed analysis of playwright and director Young Jean Lee’s theatrical production Songs of the Dragons Flying to Heaven, which premiered in 2006 at HERE Arts Center in New York City, in relationship to J. L. Austin’s concept of “performatives” introduced in his 1955 lectures at Harvard University and Eve Kosofsky Sedgwick’s notion of the “periperformative.” This analysis attends to the play’s engagement with self-alienation, a sense of failure, and enacting whiteness in the absence of white persons and reveals that the falling away of certain conventions of ethnicity in the play alludes to the limits of the subject formation without enclosing a specific group within the established perception of epistemic categories. The appearance of ethnicity characterized as infelicitous in the play offers a new way to think, “How do those who feel a distance from dominant culture move around that culture in the contemporary United States?”

장상피화생과 Helicobactor pylori와의 상관 관계에 관한 연구

이복희,김대수,이현숙,진영주,윤세진,송형근,성노현,정현용,이동호 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

In 1965 Lauren divided advanced gastric carcinoma into two main types-namely, "intestinal" and "diffuse" type carcinoma, which differ not only morphologically but also in their clinical and epidemiological characteristics. Moreover, a different histogenetic process has been postulated by many authors that intestinal type gastric carcinoma might arise from areas with intestinal metaplasia. Since Warren and Marshall reported the presence of Helicobacter pylori in the stomach in 1983, strong evidence has been obtained that there is a close relationship between Helicobacter pylori and chronic gastritis, atrophic gastritis, intestinal metaplasia, and intestinal type gastric carcinoma. To study the relationship between intestinal metaplasia and Helicobacter pylori infection, we performed gastroscopic biopsies and CLO tests taken from patients with epigastric pain. Only 87 patients with macroscopically suspected antral gastritis were included. The prevalence of intestinal metaplasia in gastric antral biopsies increased from 33.3% in the age group<30 years to 63.6% in the age group 60 years. When all patients were divided into two age groups-namely, (i)<50 years(n=41) and (ii)≥ 50 years(n-=46), intestinal metaplasia was found significantly more often in patients ≥ 50 years. ((i) 39.2%, (ii) 54.3%, p<0.0001). The prevalence of Helicobacter pylori in gastric antral biopsies decreased from 66.7% in the age group<30 years to 62.5%, in the age group in the age group 50 to 59 years. When all patients were divided into the same two age groups-namely(i)<50 years and (ii)≥50 years, the prevalence of Helicobacter pylori did not differ between two groups in a statistically significant way. The mean age of intestinal metaplasia positive patients who were also Helicobacter pylori positive was 48±11.0(mean±S.D.)years, whereas the mean age of intestinal metaplasia positive patients who were Helicobacter pylori negative was 52±9.7(mean±S.D.)years. In conclusion. although our data do not prove a causal relationship between Helicobacter pylori infection and the histogenesis of intestinal metaplasia, we suggest that Helicobacter pylori plays an important role in the development of intestinal metaplasia in the gastric mucosa. Whether Helicobacter pylori has to be present at all times during this process or is only necessary as a 'trigger', needs further research.

Development of a Chest‑Belt‑Type Biosignal‑Monitoring Wearable Platform System

Joo‑Hyeon Lee,Hyun‑Seung Cho,Jin‑Hee Yang,Sang‑Min Kim,Jeong‑Whan Lee,Hwi‑Kuen Kwak,Je‑Wook Chae 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.4

The purpose of this study was to develop a wearable platform system that can detect and acquire a soldier’s biosignals (i.e., heart activity signal, respiration rate, etc.) in a nonrestrained, unconscious manner. These detected biosignals are transmitted to a processing device to analyze and monitor the soldier’s physical status. To achieve this, textile-based heart activity electrodes and a strain gauge sensor for the respiration signal measurement were developed, and their performances in detecting each signal were verifed. These sensors were embedded in a chest belt to design a wearable platform that can simultaneously measure heart activity and respiration signals. The sensor part of the chest belt has a dual layer structure to detect high-quality signals. Stretch fabric was used on the outer layer and a respiration sensor was attached to the belt. On the inside layer, a non-stretch fabric was used as the base fabric and a heart activity-sensing electrode, that is capable of taking measurements using a modifed lead-II heart activity signal induction method, was embroidered onto the fabric. Subjects were asked to wear the chest belt, and a biosignal processor module was attached to verify the system’s performance while simultaneously acquiring the heart activity and respiration signals. More specifcally, it was confrmed that the two signals were detected in a stable. It is expected that the biosignal-monitoring wearable platform system developed in this study will be able to efectively analyze and monitor soldiers’ biosignals.

진도(전라남도, 진도군)의 거미상

김주필(Joo Pil Kim),지승환(Seung Hwan Ji),이형민(Hyung Min Lee),이형진(Hyung Jin Lee),이현동(Hyun Dong Lee) 한국거미연구소 2017 한국거미 Vol.33 No.1

2016년 7월 6일부터 8일까지 전라남도 진도군 일대를 한국거미연구회 회원인 김주필, 지승환, 이형민, 이형진, 이현동 등이 채집 조사한 결과 19과 47속 62종의 거미가 조사 연구되어 이에 보고하는 바이다. The author investigated the spiders of Jin island in July 06~08.2016. In the present paper 62 species of 47 genera belongs to 19 families were identified. These specimens are deposited at the Joo-Pil Spider Museum, Namyangju-Si, Gyeonggi-Do.

안산시화호 갈대습지공원(경기도 안산시)의 거미상

김주필(Joo Pil Kim),최한석(Han Seak Choi),예용택(Yong Taek Ye),예성훈(Seong Hun Ye),김태우(Tae Woo Kim),김대희(Dae Hee Kim),지승환(Seung Hwan Ji),지성현(Sung Hyun Ji),이형민(Hyung Min Lee),변진관(Jin Kwan Byun),조주완(Joo Whan Jo),박솔 한국거미연구소 2017 한국거미 Vol.33 No.2

한국거미연구회 회원들은 2017년 8월 3일부터 4일까지 경기도 안산시 소재 시화호 갈대습지공원을 김주필, 최한석, 예용택, 예성훈, 김태우, 김대희, 지승환, 지성현, 변진관, 이형민, 조주완, 박솔민, 이형진, 이태후, 이승후, 조윤규, 조원규, 이준이, 신정애, 이영경, 최라미, 이윤정, 이용호, 한주연, 윤혜원, 김형주 등 26명이 주야로 채집한 결과 18과 49속 71종을 발표 보고하는 바이다. From August 3 to 4 in 2017. Koran Arachnological Study members investigated the Spiders in the whole area Gyeonggi-do Ansan-si, and reported 18 Families, 49 Genus 71 Species of spiders. The collected spiders are placed (stored) in the Joo-Pil Spider museum.

운동에너지 탄에 따른 전투시스템 내의 순간화재발생에 대한 해석적 연구

이승철(Lee Seung-Chul),전우철(Jeon Woo-Chul),이해평(Lee Hae-Pyeong),이헌주(Lee Heon-Joo),이창현(Lee Chang-Hyun) 제어로봇시스템학회 2012 제어로봇시스템학회 합동학술대회 논문집 Vol.2012 No.7

본 연구는 외부 위협탄에 의한 전투시스템의 취약성을 분석하기 위한 선행연구로써, 운동에너지 탄의 종류에 따른 가상의 전투차량 내부에 적재포탄에서 순간화재발생 여부에 대한 전산해석을 수행하였다. 본 해석은 Autodyn 프로그램을 사용하였으며 순간화재발생을 판단하기 위해 Lee-Tarver ignition and growth model을 이용하였다. 본 계산에서 운동에너지 탄은 type A와 type B 두 가지로 설정하였고, 고폭탄은 COMPB, TNT 및 PBX9404를 설정하였다. 전산해석 결과, 고폭탄의 재질이 TNT와 PBX9404가 COMPB 재질보다 순간화재발생 확률이 상당히 높음을 알 수 있고 type B의 운동에너지 탄이 type A 보다 순간화재발생 확률이 다소 높음을 알 수 있었다.

서초동(서울시 서초구)의 거미상

김주필(Joo Pil Kim),정혜리(Hye ri Jeong),김대희(Dae Hee Kim),박동우(Dong Woo Park),서재웅(Jae Woong Seo),이현우(Hyun woo Lee) 한국거미연구소 2017 한국거미 Vol.33 No.1

2016년 3월 31일부터 7월 31일까지 서울특별시 서초구 서초동에 위치한 서울고등학교 교정 일대를 서울고등학교 환경생물부 부원들이 채집 조사한 결과 20과 43속 53종의 거미가 조사 연구되어 이에 보고하는 바이다. From March 31st to July 31st in 2016, members of an environmental biology club at Seoul High School located in Seocho-gu, Seoul, Dae-Hee Kim, Dong-Woo Park, Jae-Woong Seo and Hyun-woo Lee, collected 20 families 43 genera 53 species of spiders and report the result of the study here.