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cDNA Cloning and EXpression of a Cytochrome P450 1A(CYP1A) from the Pale Chub, Zacco platypus
( Hyoung Joo Jeon ),( Young Chul Park ),( Wan Ok Lee ),( Jong Ha Lee ),( Jin Hyoung Kim ) 한국하천호수학회(구 한국육수학회) 2011 생태와 환경 Vol.44 No.4
The pale chub (Zacco platypus) is generally found in Asian countries, such as Korea, Japan, and China. Nevertheless, very little information exists about the genes involved in the metabolism of xenobiotics in this species. This species is useful in monitoring the environmental impact on various pollutants in freshwater as a sentinel fish species. We cloned the full-length cUNA sequence of xenobiotic metabolizing cytochrome P450 1A (CYP1A) gene from Z. platypus and characterized it. Tissue distribution and time-dependent induction of CYP1A were studied by real-time RT-PCR. Induction pattern of CYP1A were studied by exposing the fish to an arylhydrocarbon receptor agonist, β-naphthoflavone (BNF). The liver showed the highest level of expression in basal state as well as BNF- treated fish. However, appreciable levels of expression were also recorded in Gill and kidney and the least level of expression was observed in the eye. The results of the time-course study revealed an induction in the liver, brain, and gills after 6 h and 12 h in most of the tissues. This study provides an insight into the xenobiotics metabolizing system of Z. platypus and offers baseline information for further research related to biomarker, stress, and adaptive response of this ecologically important fish species in the freshwater environment.
김주형 ( Joo Hyoung Kim ),전용철 ( Yong Cheol Jeon ),김태엽 ( Tae Yeob Kim ),은창수 ( Chang Soo Eun ),손주현 ( Joo Hyun Sohn ),한동수 ( Dong Soo Han ),장재정 ( Jae Jung Jang ),박용욱 ( Yong Wook Park ) 대한소화기학회 2008 대한소화기학회지 Vol.52 No.5
Intestinal tuberculosis is a common disease of extrapulmonary tuberculosis and should be differentiated from the inflammatory bowel diseases and malignancy such as Crohn`s disease, ulcerative colitis, amebic colitis, and colon cancer. Most frequently involved sites (75% of cases) are the terminal ileum and cecum. Other sites of involvement, in order of frequency, are ascending colon, jejunum, appendix, duodenum, stomach, esophagus, sigmoid colon, and rectum. Intestinal tuberculosis simultaneously involving the stomach and colon has been very rarely reported. Recently, we experienced a case of synchronous gastric and colonic ulcers with granulomatous inflammation. Although we did not find acid fast bacilli and the culture test was negative, empirical anti-tuberculosis therapy resulted in dramatic clinical and endoscopic improvement. We report a rare case of multifocal gastrointestinal tuberculosis with a review of literature. (Korean J Gastroenterol 2008;52:320-324)
Cho, Jin-Hyoung,Jeon, Young-Joo,Lee, Ra-Ham,Shim, Jung-Hyun,Chae, Jung-Il 한국유기농업학회 2014 韓國有機農業學會誌 Vol.22 No.1
Korean Panax ginseng C. A. Meyer (P. ginseng) is a well-known and one of the most important tonic herbs used in traditional Korean medicine. The pharmacological effects of P. ginseng have been reported by many researchers. Nevertheless, little is known between the mechanism of action and the active compounds. In this study, we performed a comprehensive proteomic analysis and protein categorization in order to understand the physiological characteristics of the major components in the adventitious roots of P. ginseng. Whole proteins extracted from the cultured adventitious roots of P. ginseng were separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Among the 1000 spots which were detected by silver staining, 113 spots were labeled and identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS). Our results showed that 40 proteins were identified among the 113 spots, with a hit ratio of 35.3%. A number of proteins identified on the 2-DE gels (30%; 16 spots) were involved in energy metabolism. These proteomic data will be helpful to better understand the physiological and pharmacological effects of P. ginseng.
Jeon, Young-Joo,Bang, Woong,Cho, Jin Hyoung,Lee, Ra Ham,Kim, Seok-Ho,Kim, Minseok S.,Park, Seon-Min,Shin, Jae-Cheon,Chung, Hak-Jae,Oh, Keon Bong,Seo, Jae-Min,Ko, Sungho,Shim, Jung-Hyun,Chae, Jung-Il Spandidos Publications 2016 International journal of oncology Vol.49 No.6
<P>Kahweol, a diterpene molecule, has antiproliferative effects on several types of human cancer cells, but whether it has apoptotic effect in non-small cell lung cancer (NSCLC) is not known. To explore this possibility, we incubated cells from two NSCLC cell lines, NCI-H358 and NCI-H1299, with different concentrations of kahweol and used the MTS assay, DAPI staining, propidium iodide staining, Annexin V staining, immunocytochemical test, and western blot analysis to characterize this molecule and the signaling pathway underlying its effects. The kahweol-treated cells showed significantly decreased cell viability, increased nuclear condensation, and an increased number of Annexin V-positive NSCLC cells. Suppression of basic transcription factor 3 (BTF3) was followed by apoptosis induced by kahweol via the ERK-mediated signaling pathway in a dose- and time-dependent manner. In addition, kahweol modulated the protein expression of BTF3 genes involved in cell-cycle regulation and apoptosis-related proteins, resulting in apoptotic cell death. Our results collectively indicated that kahweol inhibited the proliferation of NSCLC cells through ERK-mediated signaling pathways and the downregulation of BTF3.</P>
Jeon Yubin,Jin HyeJi,Kong Youjung,Cha Haeng-Geun,Lee Byung Wook,Yu Kyungjae,Yi Byongson,Kim Hee-Taek,Joo Jeong Chan,Yang Yung-Hun,Lee Jongbok,Jung Sang-Kyu,Park See-Hyoung,Park Kyungmoon 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8
Poly(3-hydroxybutyrate) (PHB) is a biodegradable and biocompatible bioplastic. Effective PHB degradation in nutrient-poor environments is required for industrial and practical applications of PHB. To screen for PHB-degrading strains, PHB double-layer plates were prepared and three new Bacillus infantis species with PHB-degrading ability were isolated from the soil. In addition, phaZ and bdhA of all isolated B. infantis were confirmed using a Bacillus sp. universal primer set and established polymerase chain reaction conditions. To evaluate the effective PHB degradation ability under nutrient-deficient conditions, PHB film degradation was performed in mineral medium, resulting in a PHB degradation rate of 98.71% for B. infantis PD3, which was confirmed in 5 d. Physical changes in the degraded PHB films were analyzed. The decrease in molecular weight due to biodegradation was confirmed using gel permeation chromatography and surface erosion of the PHB film was observed using scanning electron microscopy. To the best of our knowledge, this is the first study on B. infantis showing its excellent PHB degradation ability and is expected to contribute to PHB commercialization and industrial composting.