여러 가지 안정화 물질이 오미자 색소 추출물의 가열 변색에 미치는 영향

전향숙,김현정,조성빈 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.5

The effects of selected stabilizers and sugars on color deterioration of anthocyanin in Schizandra fructus were examined at 100℃ for 120~180 min. Among four test sugars, it was found that fructose accelerated the thermal color deterioration while maltose retarded the color deterioration by 40%. Maltodextrin and γ-cyclodextrin showed the highest stabilizing effect on the thermal color deterioration of crude pigment extract from Schizandra fructus(CPES) containing 2mg% of anthocyanin. Addition of maltodextrin or γ-cyclodextrin 5% retarded thermal color deterioration of CPES. In gel system, 5% of maltodextrin also retarded the color deterioration by 15~20% during storage at 25℃ and 35℃.

T-2 Toxin이 병아리 비장세포의 유전질 발생에 미치는 영향

전향숙,정덕화,이서래 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-

시험관 내에서 T-2 toxin이 병아리 비장세포의 blastogenesis에 미치는 영향을 살펴본 결과, B-cell mitogen인 lipopolysaccharide및 T-cell mitogen인 concanavalin A자극에 대해 T-2 toxin의 농도가 증가함에 따라 억제정도가 증가하는 경향을 나타내었다. 노출시기를 달리하여 T-2 toxin을 투여한 병아리 비장세포에서 mitogen 자극에 대한 반응을 알아 본 결과, 부화하기 전과 후에 계속 T-2 toxin에 노출시킨 실험군은 가장 영향을 많이 받은 것으로 나타났고 부화전 혹은 부화후 어느 한 시기에만 T-2 toxin에 노출된 실험군은 비교적 영향을 적게 받는 것으로 나타났다. The effects of T-2 toxin of mitogen-induced blastogenesis of chick splenic cells were investigated. The[^3H]thymidine incorporation in splenic cells stimulated by lipopolysaccharide and concanavalin A were equally inhibited as the concentration of T-2 toxin was increased. The effective dose of T-2 toxin causing a 50% reduction of[^3H]thymidine incorporation was inbetween 1.0 and 5.0 ng/㎖ for both mitogens. Mitogen-induced blastogenesis in chick splenic cells showed differences among experimental groups with different exposure time of T-2 toxin, exhibiting the most inhibition in the experimental group exposed to T-2 toxin at both embryonic and chick periods.

항암화학요법 관련 오심과 구토 완화를 위한 비약물요법에 관한 연구동향

소향숙,최자윤,조인숙,김영재,김지영,김애숙,김옥미,김춘심,김현오,설영애,안정옥,이애리,이영자 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: Purposes of this study were to understand the current trends on complementary therapy in relieving chemotherapy-induced nausea and vomiting and to suggest the future research direction. Method: Subjects were selected on CINAHL, MEDLINE, Korean Academy Data Base from 1980 to 2001 which used nausea, vomiting, chemotherapy and complementary therapy as key words in experimental studies. Eight korean articles and twenty-one international articles were analyzed in terms of general characteristics, research methods, and types of complementary therapy. Data were analyzed by using descriptive statistical methods. Result: Since 2000, researchers have more actively used complementary therapy. In subject characteristics, mean age was 35.5 years old, 45% of the researchers were performed with high level of incidence of chemotherapy induced vomiting, 14% of them set limit of consecutive cycle during research, and 65% of them did not comment the selecting criteria of sample. About 60% of them were designed post-test only control group: 35% used INV by Rhodes, 31% used Likert scale, and 24% used VAS for dependent variable. Muscle relaxation therapy was mostly applied for relief of nausea and vomiting. Conclusion: Further studies will be needed to control extrinsic variables affecting nausea and vomiting in research design and to accumulate evidence with studies applying various complementary therapies.

Chitosan 가수분해물의 in vitro 항돌연변이 활성

장현주,전향숙,이서래 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

Chitosan이 항돌연변이 활성을 나타내는 분자량범위를 알아보기 위해, Chitosan 가수분해물의 3-amino-1-methyl-5H-pyrido[4,3-b]indole(Trp-P-2), aflatoxin B_1, 2-nitrofluorene 및 4-nitroquinoline oxide에 대한 억제활성을 Salmonella typhimurium reversion assay와 SOS chromotest로 조사하였다. Chitosan을 산가수분해한 후 한외여과하여 6가지 fraction들(분자량 1,000 이하, 1,000∼3,000, 3000∼10,000, 10,000∼30,000, 30,000∼100,000, 1000,000 이상)의 chitosan 가수분해물을 제조하였다. 제조된 각 fraction의 탈아세틸화도는 큰 차이가 없었으며, 시료 자체의 pH가 복귀 돌연변이수와 SOS 유도 반응에 미치는 영향은 없는 것으로 나타났다. 얻어진 fraction별로 Salmonella typhimurium reversion assayf를 실시한 결과, Trp-P-2에 대한 항돌연변이 활성은 fraction의 6의 5% 농도에서 78%, aflatoxin B_1에 대해서는 fraction 5의 10% 농도에서 92%, 그리고 2-nitrofluorene에 대해서는 fraction 6의 5% 농도에서 51%의 최고 활성을 나타냈다. 한편 SOS chromotest에서는 Trp-P-2에 대하여 0∼54%, 4-nitroquinoline oxide에 대하여 0∼77%의 억제 활성을 나타냈다. Antimutagenic effect of chitosan hydrolysates was investigated using Salmonella typhimurium reversion assay and SOS chromotest against 3-amino-1-methyl-5H-pyrido [4,3-b]indole(Trp-P-2), aflatoxin B_1, 2-nitrofluorene and 4-nitroquinoline oxide. After partial acid hydrolysis of chitosan, six fractions of different molecular size were obtained by ultrafiltration. Chitosan hydrolysates showed antimutagenic effect of 0∼78% on Trp-P-2, 0∼92% on aflatoxin B_1 and 0∼51% on 2-nitrofluorene in Salmonella tryphimurium reversion assay. Inhibitory effect in Salmonella typhimurium reversion assay showed the highest at 5% concentration of fraction 6 on Trp-P-2, 10% concentration of fraction 5 on afaltoxin B_1 and 5% concentration of fraction 6 on 2-nitrofluorene. In SOS chromotest, chitosan hydrolysates showed anitimutagenic effect of 0∼54% on Trp-P-2 and 0∼77% on 4-nitroquinoline oxide. These results suggest that high molecular weight fraction of chitosan hydrolysates(MW>30,000)is most effective to inhibit mutagenicity of tested mutagens.

都市主婦의 結婚 適應度에 關한 硏究

金惠貞,李蓮淑,任春淑,鄭明姬,曺鏞旬,黃智暎 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-

We measured general marital adjustment of the housewives who ilve in Daegu. We analysed marital affectional expression, cohesion, consensus and satisfaction and also analysed marital adjustment according to the level of education or length or marriage. The results are as follows; 1. The level of general marital adjustment of the urban housewives was medium. 2. According to factor analysis, the level of marital affectional expression was medium, the level of marital satisfaction and consensus was high, and the level of cohesion was low. 3. The more educated, the higher the degree of marital adjustment in general. 4. The marital adjustment was lowest in housewives whose length of marriage was 9-11 years. The second lowest were 3-5 years and 21-23 years. The highest was 15-17 years.

NATURALLY OCCURRING FLAVONOIDS AND ORGANOSULFUR COMPOUNDS : THEIR ACTION ON THE BIOTRANSFORMATION OF CHEMICAL CARCINOGEN

Hyang Sook Chun 한국식품영양과학회 2001 한국식품영양과학회 학술대회발표집 Vol.50 No.-

A variety of dietary plant flavonoids and organosulfur compounds have been found to inhibit tumor development in several experimental animal models and to act through different mechanisms. One of the ways flavonoids and organosulfur compounds may inhibit carcinogenesis is by modulating the biotransformation of chemical carcinogens. As a mechanistic study, the effect of these substances on the biotransformation of benzo[a]pyrene (B[a]P) was investigated using human hepatic and extrahepatic cellular models in terms of the structure-activity relationship. Of flavonoids, flavonoids with two hydroxyl groups at the 5- and 7-position were found to inhibit the B[a]P-induced ethoxyresorufin O-deethylase (EROD) activity, a marker enzyme for cytochrome p450 1A (CYP 1A) that playa critical role in B[a]P bioactivation pathways. Flavones were more potent than corresponding flavonols, and flavonoids were more active than the corresponding isoflavonoids, suggesting the planar structure of the flavone is important in inhibiting B[a]P-induced CYP 1A activity. Organosulfur compounds such as diallyl disulfide (DADS) and diallyl trisulfide (DATS) inhibited the B[a]P-induced EROD activity by 30-90% and 70-95% at 100-1,000 μM concentration, respectively, with a mixed type inhibition pattern. Immunoblot results indicated that the B[a]P inducible CYP1A2 protein was suppressed by 100-1,000 μM of DADS and 10-100 μM of DATS. Analysis of B[a]P metabolites revealed that the levels of 7,8-diol, 9,10-diol and 4,5-diol formed were significantly reduced in the DADS and DATS treated microsomes as compared to the control. The allyl group and number of sulfur atoms of organosulfur compounds were found to be essential in dominating their modulatory potentials. In contrast, flavonoids and organosulfur compounds did not induce the activity of glutathione S-transferase (GST), which catalyses conjugation of electrophilic B[a]P metabolites with GSH. As judged by cell viability assay, an indicator of the capacity to detoxify reactive B[a]P metabolites, the cell viability was decreased by the addition of GST inhibitors, ethacrynic acid and ketoprofen, and GSH depletor, diethylmaleate but was increased by the treatment of GST enhancer, 6-propyl-n-thiouracil. Extension of these studies to other electrophile-processing enzyme showed that NAD(P)H:(quinone-acceptor) oxidoreductase (QR) activity was slightly induced by some flavonoids and organosulfur compounds. It is, therefore, implied that the modulatory action on the B[a]P bioactivation by flavonoids and organosulfur compounds may playa role in their chemopreventive activity.

Detection of T-2 Toxin in Korean Grains by ELISA

Hyang-Sook Chun,Duck-Hwa Chung,Su-Rae lee 한국식품과학회 1995 Food Science and Biotechnology Vol.4 No.2

Macrophage Stimulating Activity of Water Extract from Defatted Perilla Seeds(Perilla frutescens var.japonica)

Hyang Sook Chun,Soon-Young Hur,Byung Hee Kim,Chul Jai Kim 한국식품과학회 2002 Food Science and Biotechnology Vol.11 No.6

[S11-3] Medicinal Herbal Extracts and Their Constituents as Potential Neuroprotectants

Hyang Sook Chun 한국식품영양과학회 2005 한국식품영양과학회 학술대회발표집 Vol.2005 No.10

Microbiological and Biochemical Characterization of the Traditional Steeping Process of Waxy Rice for Yukwa (a Korean Oil-Puffed Snack) Production

Chun, Hyang-Sook,Lee, Myung-Ki,Kim, Hyun-Jung,Chang, Hyun-Joo The Korean Society of Food Science and Nutrition 2004 Preventive Nutrition and Food Science Vol.9 No.2

Selected microbiological and biochemical characteristics of the steeping process for the production of yukwa, a traditional Korean oil-puffed snack made of waxy rice, were investigated during steeping of waxy rice in water for 15 days. The lengthy steeping process was largely predominated by lactic acid bacteria (LAB), particularly, Lactobacillus and Leuconostoc. The predominat type of bacterium isolated was the Y26 strain tentatively identified as Lactobacillus plantarum. The titratable acidity of the steeping medium increased from 0.01 to 1.13%, in parallel with the decrease in pH ranging from 6.3 to 4.2 as the steeping period increased from 0 to 15 days. A high amount of lactic acid and to a much lesser extent, butyric acid, acetic acid, propionic acid, and succinic acid were detected during the steeping process. The amount of reducing sugars in the steeping medium increased from 0.61 to 10.43 mg/mL, whereas sucrose decreased from 0.46 mg% to an undetectable level. Starch degradation products including glucose, maltose and oligosaccharides ranging G3-G7 were not initially noticed, but their content increased during the steeping process until completion. However, no oligosaccharides larger than G8 were detected in the steeping medium. The activities of $\alpha$-amylase, $\beta$-amylase and protease in the steeping medium of waxy rice tended to rise increase with time during the steeping process. From these results, the lengthy steeping process in yukwa production can be characterized as the spontaneous fermentation, dominated by lactic acid bacteria, which is a necessary process for inducing biochemical modification of waxy rice.