複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

등방성 이종재료 내의 Ⅴ-노치 균열에 대한 응력강도계수 결정에 관한 연구

조상봉,정휘원,김진광 경남대학교 신소재연구소 1999 論文集 Vol.11 No.-

접합계면 V-노치 균열 문제는 고유치와 고유벡터 문제로 수식화할 수 있다. V-노치 균열첨단에서 응력특이성을 가지는 고유치가 존재한다. 상반일 등고선 적분법(RWCIM)은 고유치와 관련된 고유벡터의 계수를 구하는 한 가지의 방법이다. 상반일 등고선 적분법을 이용하여 접합계면 균열의 응력확대계수를 구하도록 시도하였다. 상반일 등고선 적분법으로 구한 응력확대계수와 경계요소법과 변위외삽법을 이용하여 구한 결과를 비교하였다. An inteiface 17-notched crack problem can be formulated as a eigenvalue problem. there are the eigenvalues which give stress singularities at the V-notched crack tip. The RWCIM is a method of calculating the eigenvector coefficients associated with eigenvalues. Obtaining the stress intensity factors for an interface crack in dissimilar materials was examined by the RWCIM. The results obtained for stress intensity factors using RWCIM were compared with those obtained by using the displacement extrapolation method and the BEM.

폐결핵의 조기진단에 있어서 이중중합효소연쇄반응법의 유용성

조성란,최영진,김용훈,이철세,김영창,김휘준 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

Background: Pulmonary tuberculosis is a great public health problem in Korea. Early detection of Mycobacterium tuberculosis (M. tuberculosis) in clinical samples becomes more and more important in the control of tuberculosis. Recently, molecular methods have been applied to early detection of M. tuberculosis. Methods: To elucidate the effectiveness of nested polymerase chain reaction (PCR) in early diagnosis of pulmonary tuberculosis, we peformed Acid-fast bacilli (AFB) stain, AFB culture, and nested PCR on 84 sputa. Results: 8 (9.5%) specimens were positive by AFB stain, 17 (20.2%) by AFB culture, and 15 (17.9%) by nested PCR. Using AFB culture as standard method, the sensitivity of AFB stain and nested PCR was 47.1% and 82.4%, respectively. The specificity of AFB stain and nested PCR was 100% and 98.5%, respectively. Conclusions: Nested PCR was more sensitive than AFB stain and had shorter processing time(24-48hrs) than AFB culture. So it may be effective to use nested PCR in order to detect M. tuberculosis when AFB smear Is negative.

전통적 해외직접투자이론의 확장을 위한 연구

문휘창,조대우 韓國國際經營學會 1990 國際經營硏究 Vol.1 No.1

지금까지 해외직접투자이론은 수없이 많은 갈래로 발전되어 왔으며, 더닝(Dunning. 1981)은 절충이론, 러그만 (Rugman, 1981)은 내부화이론 등으로 기존이론들을 통합하려고 하는 시도도 있었다. 그러나 어떤 이론도 첫째, 개도국 다국적기업의 대선진국투자 둘째, 재투자 및 투자철수 셋째, 대세계경제의 복지효과등을 포괄적으로 설명해 주지 못했다. 이에 대해 본 연구에서는 기존해외직접투자이론의 요체가 소유우위에 있다고 보고 단지 우위요인들을 나열적으로 찾아내는 것이 아니라 어떤 요인이 해외직접투자를 가능하게 하는 결정요인인지를 찾아내고자 한다. 또한 본고는 내부화요인을 투입 생산요소의 잉여요서(예: 잉여자본),투자대상국의 입지요인을 부족요인(예 : 부족노동)으로 정의하고 이에 따라 다국적기업은 최적수준이 될 때까지 이들 두 요인의 불균형분을 조정할 것이라는 논지에 입각하고 있다. 본 연구의 결과 가장 중요한 독립변수는 '불완전이용(underutilized) 자본집약도'라는 것을 찾아냈으며 특히 기술이 표준화된 산업의 경우에는 이 요인이 유일하면서 가장 유의적인 설명변수라는 것을 확인할 수 있었다. 이 결과는 미국 다국적기업에 의한 해외직접투자뿐 아니라 일본이나 개도국의 해외직접투자의 설명에도 유효할 것으로 본다.

1998-1999년에 임상 검체로부터 분리된 주요 세균의 항생제 내성

김휘준,조성란 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

Surveillance of antimicrobial resistance can provide information needs for empirical therapy of antimicrobial agents and for control of resistance. To determine the trend of antimicrobial resistance in Soonchunhyang Chunan Hospital, we analyzed in vitro susceptibility and identification data in 1998 and 1999. The results showed that cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae and 3rd-generation cephalosporin-resistant K. pneumoniae, Enterobacter cloacae, and Serratia marcescens were prevalent, that fluoroquinolone-resistant Acinetobacter baumannii had increased, and imipenem-resistant Pseudomonas aeruginosa were prevalent. Oxacillin-resistant Staphylococcus aureus and penicillin-resistant pneumococci were prevalent. An increase of imipenem-resistant P. aeruginosa and vancomycin-resistant Enterococcus faecium is a new obvious threat. To prevent the spread of multi-resistant bacteria and the emergence of new resistance, continuous monitoring of antimicrobial resistance, appropriate use of antibiotics, and control of nosocomial infection are necessary.

골반염증성 질환과 질염을 가진 환자의 진단과 추적관찰에서 Chlamydia trachomatis의 검출을 위한 이중 중합효소 연쇄반응의 적용

이진우,조현철,최규연,배동한,김휘준 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Objectives : PCR has generally been more sensitive than the conventional microbiological test for the detection of C.trachomatis which has been a leading cause of sexually transmitted disease. The purpose of this study was to evaluate the prevalence of C.trachomatis infection in the patients with pelvic imflammatory disease(PID) and the usefulness of nested PCR as a follow-up test in patiens with C. trachomatis infection after administration of antibiotics. Methods: Cervical swab specimens were obtained from 187 patients with PID and vaginitis attending the Soonchunhyang Chunan Hospital. From the C. trachomatis positive patients, cervical swab specimens were obtained once a week after treatment until PCR tested negative. We performed the nested PCR with Chlamydia-CR kit(Bioneer, Korea.) Results: From 6(5.8%) of 187 cervical swab specimen, C. Trachomatis was detected by nested PCR. These patients were treated with doxycycline and metronidazole for 7 to 14 days. All of 6 C. trachomatis positive patients revealed PCR-negative immediately after the completion of antibiotics treatment. Conclusions: With the above results, the nested PCR is a relatively simple, fast, and practical tool for the detection of C. trachmatis and may be a useful follow-up test for monitoring the treatment effect.

Thrombopoietin이 제대혈의 CD34 양성 조혈모세포의 거핵구계 세포분화에 미치는 영향

서진태,조경삼,윤휘중,김시영,이우인 경희대학교 2000 慶熙醫學 Vol.16 No.2

성인 급성 골수성 백혈병에서 6-Thioguanine, Cytarabine, Doxorubicin(TAD) 3제 복합화학요법의 효과 : 최종보고

최환석,윤휘중,우인숙,박지훈,김정희,김시영,조경삼 大韓血液學會 1995 大韓血液學會誌 Vol.30 No.2

Licochalcone A, a natural chalconoid isolated from Glycyrrhiza inflata root, induces apoptosis via Sp1 and Sp1 regulatory proteins in oral squamous cell carcinoma.

Cho, Jung Jae,Chae, Jung-Il,Yoon, Goo,Kim, Ka Hwi,Cho, Jin Hyoung,Cho, Seung-Sik,Cho, Young Sik,Shim, Jung-Hyun Lychnia 2014 International journal of oncology Vol.45 No.2

<P>Licochalcone A (LCA), a chalconoid derived from root of Glycyrrhiza inflata, has been known to possess a wide range of biological functions such as antitumor, anti-angiogenesis, antiparasitic, anti-oxidant, antibacterial and anti-inflammatory effects. However, the anticancer effects of LCA on oral squamous cell carcinoma (OSCC) have not been reported. Our data showed that LCA inhibited OSCC cell (HN22 and HSC4) growth in a concentration- and time-dependent manner. Mechanistically, it was mediated via downregulation of specificity protein 1 (Sp1) expression and subsequent regulation of Sp1 downstream proteins such as p27, p21, cyclin D1, Mcl-1 and survivin. Here, we found that LCA caused apoptotic cell death in HSC4 and HN22 cells, as characterized by sub-G(1) population, nuclear condensation, Annexin V staining, and multi-caspase activity and apoptotic regulatory proteins such as Bax, Bid, Bc1-(xl), caspase-3 and PARR Consequently, this study strongly suggests that LCA induces apoptotic cell death of OSCC cells via downregulation of Sp1 expression, prompting its potential use for the treatment of human OS CC.</P>

Reduced beta-catenin expression in the hippocampal CA1 region following transient cerebral ischemia in the gerbil.

Cho, Jeong-Hwi,Yan, Bing Chun,Lee, Young Joo,Park, Joon Ha,Ahn, Ji Hyeon,Kim, In Hye,Lee, Jae-Chul,Kim, Young-Myeong,Lee, Bonghee,Cho, Jun Hwi,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2013 Neurochem Res Vol.38 No.5

<P>Beta-catenin, a transcription factor, plays a critical role in cell survival and degradation after stroke. In this study, we examined changes of expression in beta-catenin in the hippocampal CA1 region of the gerbil following 5 min of transient cerebral ischemia. We observed neuronal damage using cresyl violet staining, neuronal nuclei immunohistochemistry and Fluro-Jade B immunofluorescence. Four days after ischemia-reperfusion (I-R), most of pyramidal cells in the CA1 region were damaged. In addition, early damage in dendrites was detected 1 day after I-R by immunohistochemical staining for microtubule-associated protein 2 (MAP-2), and MAP-2 immunoreactivity was hardly detected in the CA1 region 4 days after I-R. We found that beta-catenin (a synapse-enriched cell adhesion molecule) was well expressed in dendrites before I-R. Its immunoreactivity was well colocalized with MAP-2. Chronological change of beta-catenin immunoreactivity was novelty in the present study. Twelve hours after I-R, its immunoreactivity was decreased in the stratum radiatum of the CA1 region, however, its immunoreactivity was increased 1 and 2 days after I-R, and decreased sharply 4 days after I-R. However, we did not find any change in beta-catenin immunoreactivity in the CA2 and CA3 region. In brief, we suggest that early change of beta-catenin expression in the stratum pyramidale of ischemic hippocampal CA1 region is associated with early dendrite damage following transient cerebral ischemia.</P>