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      • KCI등재

        Isolation of L-theanine from Tea Solution by Cation Exchange Resin in Batch and Fixed Bed Column

        Jian-Hui Ye,Yi-Wen Luo,Hui-Ling Liang,Jian-Liang Lu,Jing Jin,Yue-Rong Liang,Xin-Qiang Zheng,Xian-Yang Luo 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.2

        L-Theanine, a bioactive compound in tea, was isolated from tea solution using cation exchange resin no.732. The adsorption of L-theanine by cation exchange resin no.732 fit the Langmuir isotherm model and was a monolayer molecular interaction process. Thermodynamic studies revealed that the adsorption of L-theanine by resin no.732 was an exothermic and spontaneous physically driven process. The adsorption capacity was influenced by temperature, initial concentration, and pH. The L-theanine adsorption capacity under conditions at room temperature,pH 4.73, and initial L-theanine concentration 18 g/L was 241.731 ± 3.679 mg/g. The Thomas model was fit to describe the column adsorption data at different flow rates and initial concentrations. The L-theanine adsorbed by resin no.732 could be desorbed by 0.134 mol/L Na2HPO4aqueous solution with a recovery rate of 84.96%. These findings indicate that resin no.732 was a promising material for isolating L-theanine from tea solution.

      • KCI등재

        Serum fibrosis index-based risk score predicts hepatocellular carcinoma in untreated patients with chronic hepatitis B

        ( Lilian Yan Liang ),( Hye Won Lee ),( Vincent Wai-sun Wong ),( Terry Cheuk-fung Yip ),( Yee-kit Tse ),( Vicki Wing-ki Hui ),( Grace Chung-yan Lui ),( Henry Lik-yuen Chan ),( Grace Lai-hung Wong ) 대한간학회 2021 Clinical and Molecular Hepatology(대한간학회지) Vol.27 No.3

        Background/Aims: Serum fibrosis scores comprised of common laboratory tests have high utility to assess severity of liver fibrosis. We aimed to derive and validate a hepatocellular carcinoma (HCC) risk score based on serum fibrosis scores to predict HCC in treatment-naive chronic hepatitis B (CHB) patients. Methods: Fifteen thousand one hundred eighty-seven treatment-naive adult CHB patients were identified to form the training cohort in this retrospective study. Individual fibrosis score was included to construct a new HCC prediction score. The score was externally validated in an independent treatment-naive Korean CHB cohort. Results: 180/15,187 patients (1.2%) in training cohort and 47/4,286 patients (1.1%) in validation cohort developed HCC during a mean follow-up of 52 and 50 months, respectively. The newly developed HCC risk score, Liang score, is composed of gender, age, hepatitis B virus DNA, fibrosis-4 (FIB-4) index, and ranges from 0 to 22. Area under the time-dependent receiver operating characteristic curve of Liang score was 0.79 (95% confidence interval, 0.70-0.89). A cutoff value of nine provided an extremely high negative predictive value of 99.9% and high sensitivity of 90.0% at 5 years in the validation cohort. Patients with Liang score ≤9 had HCC incidence <0.2% per year in both training and validation cohorts, in whom HCC surveillance might be exempted. Conclusion: A novel HCC risk score, Liang score, based on FIB-4 index, is applicable and accurate to identify treatment-naive CHB patients with very low risk of HCC to be exempted from HCC surveillance. (Clin Mol Hepatol 2021;27:499-509)

      • Radiosensitivity Enhancement by Arsenic Trioxide in Conjunction with Hyperthermia in the EC-1 Esophageal Carcinoma Cell Line

        Cui, Yan-Hui,Liang, Hai-Jun,Zhang, Qing-Qin,Li, Si-Qing,Li, Xiao-Rui,Huo, Xiao-Qing,Yang, Qing-Hui,Li, Wei-Wei,Gu, Jian-Fa,Hua, Qin-Liang,Lu, Ping,Miao, Zhan-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

        Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

      • SCISCIESCOPUS

        Dinuclear Valence Tautomeric 1,2-Semiquinonato/Catecholatocobalt Complexes Containing 1,1,4,7,10,10-Hexamethyltriethylenetetramine

        Liang, Hui,Na, Young Mee,Chun, In Sung,Kwon, Soon Sik,Lee, Young-A,Jung, Ok-Sang Chemical Society of Japan 2007 Bulletin of the Chemical Society of Japan Vol.80 No.5

        <P>Dinuclear 1,2-semiquinonato/catecholatocobalt complexes containing 1,1,4,7,10,10-hexamethyltriethylenetetramine (hmdeta) as a potential tetradentate N<SUB>4</SUB> coligand, [Co<SUB>2</SUB>(hmteta)(dbbq)<SUB>4</SUB>], (dbbq = 3,5- and 3,6-di-<I>tert</I>-butyl-1,2-benzoquinone (3,5-dbbq and 3,6-dbbq)) were synthesized and characterized. The crystal structures proved that [Co<SUB>2</SUB>(hmteta)(3,6-dbbq)<SUB>4</SUB>]·2C<SUB>6</SUB>H<SUB>5</SUB>CH<SUB>3</SUB> ([3,6]) exists as low spin [(3,6-dbsq)(3,6-dbcat)Co<SUP>III</SUP>(hmteta)Co<SUP>III</SUP>(3,6-dbsq)(3,6-dbcat)] (3,6-dbsq = 3,6-di-<I>tert</I>-butyl-1,2-semiquinonato; 3,6-dbcat = 3,6-di-<I>tert</I>-butylcatecholato), while [Co<SUB>2</SUB>(hmteta)(3,5-dbbq)<SUB>4</SUB>]·C<SUB>6</SUB>H<SUB>5</SUB>CH<SUB>3</SUB> ([3,5]) approximates to [(3,5-dbsq)<SUB>2</SUB>Co<SUP>II</SUP>(hmteta)Co<SUP>II</SUP>(3,5-dbsq)<SUB>2</SUB>] in the solid state at ambient temperature. On the basis of the effective magnetic moments, the [Co<SUP>III</SUP>] → [Co<SUP>II</SUP>] conversion of [3,6] underwent a relatively abrupt transition around 330 K while that of [3,5] occurs in a wide range of temperature. Electronic absorption spectra showed that [3,6] shifts predominantly to [Co<SUP>III</SUP>] whereas [3,5] shifts to [Co<SUP>II</SUP>] valence tautomer in solution at room temperature. The charge distribution of [3,6] exhibited significant solvent effects at room temperature. These prominent features between [3,5] and [3,6] appeared to be associated with difference between delicate electronic and steric effects of the two dbbq ligands.</P>

      • KCI등재

        Comparative Study on the Competitiveness of Film Industry among Korea China and Japan

        Liang-Hui,Kim, Kyung-Tae 한국경영컨설팅학회 2017 경영컨설팅연구 Vol.17 No.1

        본 논문은 한중일 영화 산업의 경쟁력 비교를 통해서 각국의 영화 신업의 장단점을 제시하며 향후 발전 방향을 살펴 본다. 본 연구에서 사용한 모든 데이터는 모두 국제 기관 및 각국 정부가 공개한 통계 자료에서 수집하고 다이아몬드 모델을 이용해 분석하였다. 구체적인 결과를 살펴보면, 일본의 영화산업 경쟁력 지수는 0.87로 가장 높게 나타났으며 한국은 0.72,중국은 0.55로 비교적 낮은 수준이다. 본 비교 연구를 통해 한국의 가장 큰 문제는 이익공간이 낮은 것을 알 수 있으며 중국은 인적 자원이 부족 하고 일본은 해외시장 개발이 필요로 한다는 것을 알 수 있다. 따라서 미래 영화 산업 경쟁력을 높이기 위해 본 연구에서 이런 문제를 대비하기 위한 해결법 또한 제시해 보았다. The aim of this paper is to analyze and compare the film industry competitiveness among South Korea, China and Japan in order to explore possible breakthrough points in the film industry which, in turn, has a significant impact on the development of their cultural industry. This study collected data and materials from existing competitive reports on current statistics provided by international institutions and government departments. Mainly, Porter’s diamond model was used to analyze and compare the competitiveness of the film industry. This research found that the competitiveness index of the film industry in three countries was 0.87 in Japan, 0.72 in South Korea, and 0.55 in China, respectively. Comparing competitiveness of film industries in these countries can reveal their advantages and disadvantages, and yields insights for future development. The long-term development of South Korean film industry lies in the improvement of profits. For China, professional talent training is an urgent issue, while Japan’s low income from the domestic market is causing a problem.

      • KCI등재

        Compound glycyrrhizin injection for improving liver function in children with acute icteric hepatitis: A systematic review and meta-analysis

        Liang Shi-Bing,Hou Wen-Bin,Zheng Ruo-Xiang,Liang Chang-Hao,Yan Li-Jiao,Wang Hao-Nan,Cao Hui-Juan,Han Mei,Robinson Nicola,Liu Jian-Ping 한국한의학연구원 2022 Integrative Medicine Research Vol.11 No.1

        Background: Compound glycyrrhizin injection (CGI) is a preparation with glycyrrhizin as the main active ingredient extracted from licorice. As clinical trials suggest that CGI is effective in improving liver function for acute icteric hepatitis in children (AIHC), this systematic review aimed to evaluate and verify its therapeutic effects and safety. Methods: Six electronic databases were searched from their inception to 15 May 2021. Randomized controlled trials (RCTs) assessing therapeutic effects and safety of CGI for AIHC were included. The risk of bias for each trial was assessed using the Cochrane Risk of Bias Tool 2.0. Primary outcomes were indexes related to liver function, including total bilirubin (TBiL), alanine aminotransferase (ALT) and aspartate transaminase (AST). RevMan 5.4 software was used for data analyses. The certainty of the evidence was assessed using the online GRADEpro tool. Results: Six RCTs involving 608 children were included. The overall bias was assessed as having “high risk of bias” in all trials. All trials compared the combination of CGI and conventional western medicine (CWM) with CWM alone. Regarding the effects of CGI for AIHC, results showed that CGI plus CWM was superior to CWM alone in reducing the levels of TBiL (mean difference (MD) = -8.19 mmol/L, 95% CI -9.86 to -6.53), ALT (MD = -24.09 U/L, 95% CI -30.83 to -17.34) and AST (MD = -18.67 U/L, 95% CI -21.88 to -15.45). No trial reported adverse events. The certainty of the evidence for outcomes were all evaluated as low or very low. Conclusion: CGI may have adjuvant therapeutic effects on improving the liver function of children with AIHC. There is no evidence to determine the safety of CGI for AIHC. As current evidence is weak, further well-designed RCTs are required for verification of the therapeutic effects of CGI. Background: Compound glycyrrhizin injection (CGI) is a preparation with glycyrrhizin as the main active ingredient extracted from licorice. As clinical trials suggest that CGI is effective in improving liver function for acute icteric hepatitis in children (AIHC), this systematic review aimed to evaluate and verify its therapeutic effects and safety. Methods: Six electronic databases were searched from their inception to 15 May 2021. Randomized controlled trials (RCTs) assessing therapeutic effects and safety of CGI for AIHC were included. The risk of bias for each trial was assessed using the Cochrane Risk of Bias Tool 2.0. Primary outcomes were indexes related to liver function, including total bilirubin (TBiL), alanine aminotransferase (ALT) and aspartate transaminase (AST). RevMan 5.4 software was used for data analyses. The certainty of the evidence was assessed using the online GRADEpro tool. Results: Six RCTs involving 608 children were included. The overall bias was assessed as having “high risk of bias” in all trials. All trials compared the combination of CGI and conventional western medicine (CWM) with CWM alone. Regarding the effects of CGI for AIHC, results showed that CGI plus CWM was superior to CWM alone in reducing the levels of TBiL (mean difference (MD) = -8.19 mmol/L, 95% CI -9.86 to -6.53), ALT (MD = -24.09 U/L, 95% CI -30.83 to -17.34) and AST (MD = -18.67 U/L, 95% CI -21.88 to -15.45). No trial reported adverse events. The certainty of the evidence for outcomes were all evaluated as low or very low. Conclusion: CGI may have adjuvant therapeutic effects on improving the liver function of children with AIHC. There is no evidence to determine the safety of CGI for AIHC. As current evidence is weak, further well-designed RCTs are required for verification of the therapeutic effects of CGI.

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