Random Amplified Polymorphic DNA Typing에 의한 동물과 사람 유래 Vancomycin 내성 장구균의 유전학적 비교

조윤상,이희수,김종만,류판동,박용호,유한상,이문한 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1

It has been recently reported the possibility in the transfer of antimicrobial resistant to other animals and humans. In particular, the occurrence of vancomycin resistant enterococci (VRE), which have been known as a principal antimicrobial resistant bacteria in humans, has been increased as a pathogen of nosocomial infections. And then animal VRE were suspected as an origin of human VRE. In this work, we investigated the relatedness between VRE from animals and humans through random amplified polymorphic DNA (RAPD) typing. The genetic relatedness of vanA, vanB, vanC-1 and vanC-2 genotypes was examined by RAPD typing, respectively. van4 genotypes have not been shown high genetic relationship each other, containing VRE from humans (n = 8) and chickens (n = 5). In vanB genotypes from humans (n = 3), 2 isolates were made up one cluster, shown 77.8% homology. Chicken isolates of vanC-1 genotypes (n = 11) were constituted 2 clusters and the homology of 2 clusters was 81.8% and 80.0%, respectively. In case of vanC-2 genotypes (n = 19), 2 isolates from pigs have been shown 76.9% homology. But, between VRE from animals and humans, the isolates of high genetic relationship could not be found and RAPD typing was a useful epidemiological method that could be confirmed the genetic relationship among VRE.

동물과 사람유래 Vancomycin 내성 장구균의 항균제 감수성 비교

조윤상,이희수,김종만,류판동,박용호,유한상,이문한 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1

It has been recently reported the possibility in the bansfer of antimicrobial resistance to other animals and humans. In particular, vancomycin resistant enterococci (VRE), which have been known as a principal antimicrobial resistant bacteria in humans, have been increased as a pathogen of nosocomial infections. And then animal VRE were suspected as an origin of human VRE. In this study, we isolated Enterococcus spp. from animals, identified by bio- chemical tests, examined for antimicrobial susceptibility, and then compared the antimicrobial susceptibility of VRE among each other as well as human VRE. Enterococcus iecium (29%) was predominant in Enterococcus species (n=122) isolated from animal feces in this study. E. hirae, E. iecalis, E. casseliflam and E. gallinarum were also isolated as rates of 24%, 21%, 16% and 7%, respectively. The resistance of enterococci to penicillin and tetracycline were 66% and 78%, respectively, and the susceptibility of them to chloramphenicol was 66%. Antimicrobial susceptibility test has shown that 91% of VRE from humans (n=11) was susceptible to chloramphenicol and all resistant to penicillin, rifampin and streptomycin. Seventy-five percentage of VRE from chickens (n = 12) was susceptible to rifampin and resistances of them to penicillin, tetracycline and sbeptomycin were 75%, 83%, and l00%, respectively. Therefore, we confirmed the difference of antimicrobial susceptibility from animals and humans, and the antimicrobial susceptibility test could be one of the simple and useful methods for the epidemiological survey of VRE.

Platinum sensitive 재발성 난소암에서 Carboplatin/Paclitaxel의 효과

장혜진,유희석,임윤경,문세희,장기홍,이정필 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.4

목적 : 재발성 난소암에서 platinum 감수성을 기준으로 2차 항암요법으로 사용한 Carboplatin과 Paclitaxel의 효과를 알아보기 위하여 본 연구를 진행하였다. 연구방법 : 1998년 1월 1일부터 2003년 8월 31일까지 만 5년 8개월 간 아주대학교병원 산부인과에 재발성 난소암으로 입원한 환자 중에서 최초 수술로 난소암을 확진한 후 Cisplatin 또는 Carboplatin 등 platinum 제재가 포함된 항암치료를 받은 후 6개월 이후에 재발한 환자 29명을 대상으로 Carboplatin은 5 AUC를 그리고 Paclitaxel은 175 mg/m^(2)를 투여하였으며, 최소 3회에서 최고 16회까지 투여하였다. 치료효과의 판정은 CT, MRI, Ultrasonography등 영상진단상 암종괴의 크기가 완전히 소실되었을 경우를 완전반응(complete response)으로, 50%이상 감소되었을 경우를 부분반응(partial response)으로, 50% 미만으로 감소되었거나 20% 미만 증가하였을 경우를 불변(stable disease)으로, 그리고 20% 이상 증가되었을 경우를 진행(progressive disease)로 정의하였다. 결과 : 대상 환자에서 완전반응은 6명(21%), 부분반응은 11명(38%), 그리고 불변은 5명(17%)로 전체 반응율 (overall response rate)은 59% 그리고 임상적 유용성(clinical benefit=CR+PR+SD)은 76%이었다. 결론 : 재발성 난소암환자에서 기왕에 사용한 Platinum 제재에 민감한 환자에서는 Carboplatin과 Paclitaxel의 병행 투여가 효과적이었다. Objective : To ascertain the effect of Carboplatin and Paclitaxel combination as a second line regimen for recurrent ovarian carcinoma based on platinum sensitivity. Methods : From January 1st, 1998 through to August 31st, 2003, 29 patients were retrospectively reviewed who had previously underwent primary cytoreductive surgery and adjuvant chemotherapy with Cisplatin or Carboplatin for ovarian carcinoma, and who had later recurred during follow up. All 29 patients had recurred at least 6 months after therapy, and thereafter received second line chemotherapy consisting of Carboplatin (5 AUC) and Paclitaxel (175 mg/㎡) for a minimum of least 3 cycles and a maximum of 16 cycles. Treatment efficacy was assessed by imaging studies including Computerized tomography, Magnetic Resonance Imaging, or Ultrasonography. Complete response was determined as complete disappearance of tumor, partial response at least than 50% reduction in tumor volume, stable disease as less than 50% reduction or less than 20% increase in tumor volume, and progressive disease as more than 20% increase. Results : There was complete response in 6 (21%) patients, partial response in 11 (38%), stable disease in 5 (17%), showing an overall response rate of 59%. The clinical benefit (CR+PR+SD) was 76%. Conclusion : The combination of Carboplatin and Paclitaxel administration seems to be an effective mode of second line therapy in patients with recurrent ovarian carcinoma who had been sensitive to prior platinum based chemotherapy.

충북지역 도시방재계획의 도시계획적 진단과 발전방향 : 도시계획서 분석 및 전문가 의견조사를 중심으로

백기영,황희연,박병호,이만형,황재훈,류을렬 永同大學校 1999 硏究論叢 Vol.5 No.1

본 연구는 도시방재계획의 현황을 도시계획적 차원에서 진단하고 그 발전방향을 도출하고자 하는 목적하에, 충청북도의 조례와 지침중 도시방재 관련 사항을 정리하며, 충청북도 지방도시계획위원회 회의록 및 관련자료를 검토하여 방계계획의 취급 수준을 파악하였다. 실제 도시계획 상황에 대한 진단은 도시계획 보고서를 대상으로 도시방재계획의 위상을 분석하고 평가하였다. 또한 도시방재계획의 위상과 관련 전문가의 인식수준을 평가하고 이를 바탕으로 향후 바람직한 도시방재계획의 위상을 설정하기 위해 필요한 사항을 분석하고자, 도시방재에 관한 일반의식 수준, 도시재해에 관한 대처방안, 도시방재계획의 평가방향 등을 중심으로 설문조사를 실시하였다. 이상과 같은 충북지역의 방재계획에 대한 도시계획적 진단을 바탕으로 도시방재계획 관리체계의 방향 도출과 도시방재계획의 향후방향 제안을 시도하였다. This paper examines the status quo of the existing urban disaster prevention plans in Chungbuk Province and suggests alternative orientations for the future application. Its methodological themes center around the systematic compilation and accountability of disaster-related ordinances, mostly hinged on evaluation of major disaster items documented in various urban master or revision plans. In addition, it recommends the concrete guidelines and contents in disaster prevention plans, both of which are based on the aggregated results of expert opinion survey in and around Chungbuk Province. In order to tackle with diverse and unpredictable disasters which are well observed in modern urban society, this paper urges that the relatively weak legal and planning standards of the existing disaster prevention plans have to be immediately revised. For instance, the paper vividly shows that the present dimension of disaster prevention plans within the existing master plans and perception of specialist are not satisfactory mainly because of the internal limitation of the existing plans. Secondly, it seems imperative for planners to pay attention to systematic operation and management, even starting from the early development stage of urban disasters. Lastly, it proposes the alternative checklists given to measure the suitability of the disaster plans.

삼출성 중이염 환자에서의 혈중 및 아데노이드 면역글로불린 E의 면역혈청학적 연구

장혁순,강성호,유창수,김성현,이병돈,박영진,윤세동,안희영,강주원 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Otitis media with effusion (OME) is one of the most common disease in children but its pathogenesis is not clear. In particular the role of allergy has revealed conflicting support with widely varing opinions as to its importance. Within recent years, striking biochemical and immunochemical analytic techniques have opened a new approach the study of otitis media with effusion. So we focused on distributions of IgE in adenoid tissue and serum IgE levels. Authors compared the serum IgE and the distribution of IgE in adenoid tissue between the 20 patients underwent tonsillectomy, adenoidectomy and ventilating tube insertion and control group of 11 patients underwent tonsillectomy and adenoidectomy. The results were as follows; 1. In the distribution of IgE in adenoid tissue, there was a significant increase in the group of patients with OME compared to the control group (P<0.01). 2. In the analysis of serum IgE levels, the levels of IgE in the OME patients an control group were higher than normal level, although the level of IgE in the OME patients were less than that of control group, there was no significance P>0.05).

랫드에서 미생탕의 4주 반복투여독성

김태명, 류재면, 서임권, 박동선, 조정희, 장용훈, 김윤배, 황석연 충북대학교 동물의학연구소 2009 Journal of Biomedical and Translational Research Vol.10 No.1

본문참고

뇌하수체 종양 제거술 시행 후 뒤늦게 발생한 횡문근 융해증과 동반된 중추성 열

장제혁,최규남,김일환,노은지,김윤정,유충헌,고정해,박봉수,김태균,권민정,이순희,박정현 인제대학교 2008 仁濟醫學 Vol.29 No.-

Body temperature is controlled by thermoregulatory center of the hypothalamus. We report a case of 24 years old man with central fever. He was subjected to a partial excision of pituitary tumor compressing optic chiasm four years ago. He has received hormonal therapy for panhypothyroidism after removal of pituitary tumor. And He received gamma knife operation for partially contrast-enhancing masses in suprasellar and both hypothalamic areas that is probably postoperatively remnant or recurrent tumor of pituitary adenoma. One year after gamma knife operation, he presented with a febrile syndrome of unknown origin including rhabdomyolysis. All usual investigations proved negative. We diagnosed him as central fever with rhabdomyolysis. He received medical ICU care with cooling bed, ice pack. And his symptom improved. Postoperative hyperthermia may result following resection of the pituitary tumor. When central fever is suspected taking note of past history, a quick recognition of course of fever can help reduce the using of unnecessary antibiotics and hospital stay.

Mutant p53-Notch1 Signaling Axis Is Involved in Curcumin-Induced Apoptosis of Breast Cancer Cells

Yun-Hee Bae,Jong Hyo Ryu,Hyun-Joo Park,Kwang Rok Kim,Hee-Jun Wee,Ok-Hee Lee,Hye-Ock Jang,Moon-Kyoung Bae,Kyu-Won Kim,Soo-Kyung Bae 대한생리학회-대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Notch1 has been reported to be highly expressed in triple-negative and other subtypes of breast cancer. Mutant p53 (R280K) is overexpressed in MDA-MB-231 triple-negative human breast cancer cells. The present study aimed to determine whether the mutant p53 can be a potent transcriptional activator of the Notch1 in MDA-MB-231 cells, and explore the role of this mutant p53-Notch1 axis in curcumin-induced apoptosis. We found that curcumin treatment resulted in an induction of apoptosis in MDA-MB-231 cells, together with downregulation of Notch1 and its downstream target, Hes1. This reduction in Notch1 expression was determined to be due to the decreased activity of endogenous mutant p53. We confirmed the suppressive effect of curcumin on Notch1 transcription by performing a Notch1 promoter-driven reporter assay and identified a putative p53-binding site in the Notch1 promoter by EMSA and chromatin immunoprecipitation analysis. Overexpression of mutant p53 increased Notch1 promoter activity, whereas knockdown of mutant p53 by small interfering RNA suppressed Notch1 expression, leading to the induction of cellular apoptosis. Moreover, curcumin-induced apoptosis was further enhanced by the knockdown of Notch1 or mutant p53, but it was decreased by the overexpression of active Notch1. Taken together, our results demonstrate, for the first time, that Notch1 is a transcriptional target of mutant p53 in breast cancer cells and suggest that the targeting of mutant p53 and/or Notch1 may be combined with a chemotherapeutic strategy to improve the response of breast cancer cells to curcumin.

Transgenic peppers tolerant to a new CMV pathotype

Yun Hee Lee,Min Jung,Sun Hee Shin,Jin Sook Yoon,Soon Ho Choi,Dong Bo Shim,Nam Han Her,Jang Ha Lee,Ki Hyun Ryu,Kee Yoeup Paek,Chee Hark Ham 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.38 No.1

Poster Session:PS 1210 ; Cardiology : Thrombolytic Treatement : Lower Limb Ischemia after Bee Sting

( Hee Yun Ryu ),( Jae Woong Choi ),( Sung Kee Ryu ),( Ji Young Park ),( Sueng Hwan Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Bee venom cause urticaria and localized pain; however, severe symptoms include anaphylasix, cardiovascular collapse, and death. Following case study is about arterial thrombotic occlusion and severe ischemia in lower limb few hours after bee stings. The patient (58/M) was stung 5 times ( frontal, inf orbital, Lt. knee). After 1 hour, the patient felt pallor, pain, coldness in left toe and did not have dorsalis pedis pulsation. Lab fi nding suggested diseminated intravascular coagulation. ECG, chest X-ray, cardiac doppler echo showed no abnormality. Whole body CT was conducted and thrombus was observed in internal iliac artery, lower extremity aa. Conduction of PTA, insertion of localized cathetering urokinase, and prescription of aspirin, plavix on the fi rst day of admission. The next day, patient showed thrombocytopenia and prolonged INR, but dorsalis pedis artery pulsation became palpable. After 5 days of treatment, CT angiography was conducted for f/u and normal blood vein and artery was observed. And lab fi nding becoming normalization. Ischemic change from bee sting is not well known, and there is no guideline for surgery and medication; there is no article about treatment in Korea. Our case was the fi rst to try emergency PTA to a patient with thrombus caused by bee sting. CT angiography was used to find the location and severity of the thrombus. We used PTA to catherterize a channel in the artery, and injected urokinase (1/3 of the recommended dosage). We try to prevent DIC progression from decrease of medication and targeting thrombolysis. Then antiplatelet drug was prescribed and after few days normal blood artery was observed from CT angiography. Wrong thrombolysis treatment can provoke progression to systemic disease. We focused on targeted medication dosage and location for treatment and was able to achieve good result.