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미치광이풀 모상근 배양에서 Tropane Alkaloid 생산성 증진을 위한 최적 생물학적 엘리시터 선발
정희영,강승미,강영민,김용덕,양재경,정영관,최명석 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
Scopolamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supematant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.
高X線 吸收유리에 있어서 BaO, SrO의 含量變化가 液相溫度에 미치는 影響
李卿喜,梁在翊,金德文,姜元浩 明知大學校 産業技術硏究所 1987 産業技術硏究所論文集 Vol.6 No.2
The liquidus temperature was observed according to the change of content BaO 6 W/O ~ 12W/O and SrO 1 W/O~4W/O in R₂O-PbO-Al₂O₃- (BaO+SrO+SiO₂) glass system. The liquidus temperature varied between 810°C ~ 950°C in these glasses. The effect of BaO & SrO content to the liquidus temperature of the glasses are as follows. 1. The variation of the liquidus temperature were remarkable by the content of SrO in the range of BaO contents from 6 W/O to 8 W/O. 2. The minimum liquidus temperature is located at the composition of BaO 8 W/O SrO 4 W/O SiO₂ 68 W/O.
관광자원으로서의 자연휴양림의 관광환경분석과 문제점에 관한 연구 : 유명산, 대관령, 청태산 중심으로
김종은,양덕희 慶熙大學校 觀光産業情報硏究院 2000 觀光産業情報論集 Vol.2 No.-
Rapid growth in living standards and leisure time has increased the demand for leisure facilities, and concentration of cities and environmental pollution have created more demand for green space. Now people think of forestry more as the space for rest and leisure than as that for wood production, The demand for the forestry of the city people, who want to escape from the routine, has exploded and will continue to grow. Forestry Administration started to develop 'forest for recreation' in 1988. As of December 1999, it has designated about 176 places as forest for recreation, 83 of which have been already completed. The government mobilized the people in an unprecedented afforestation campaign to plant trees in the barren hills and mountains at the beginning of the national foundation. The successful campaign has brought dense woods back, Beautiful valleys and natural ecology have recovered its value as tourism resources. The natural forest for recreation has created variety of benefits for general public. It helps individuals to relax and thus increase productivity and living standard. It has helped to meet the demand for leisure not only as a place of out-door activity but as a place for nature exploration. It also helps to enhance the underdeveloped economy and cultural standard of remote isolated villages. Short history of recreation forest uncovered a lot of problems in the areas of development, management and operation, which requires urgent attention.
Duck-Jae Koh,Yang-Hee Kim,Deog-Gon Kim,Jin-Yong Lee,Kyung-Tae Lee 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.6
The purpose of this study was to investigate the effects of Kyung Hee Allergic Disease Herbal Formula (KAHF) on atopic dermatitis (AD) and its mode of action. Our clinical study showed KAHF reduced Severity Scoring of Atopic Dermatitis (SCORAD) indexes and subjective symptom scores. In parallel, the decreased levels of interferon (IFN)-γ and interleukin (IL)-5 in serum, which contributed to its AD-mitigating effect was observed. To reveal the underlying mechanisms of KAHF in AD, its anti-inflammatory effect on lipopolysaccharide (LPS)-induced responses in RAW 264.7 cells was examined. KAHF was found to significantly inhibit the productions of nitric oxide (NO), prostaglandin E₂ (PGE₂), and IL-1β in LPS-stimulated RAW 264.7 macrophages. Consistently, KAHF potently inhibited protein and mRNA expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, KAHF inhibited LPS-induced activation of nuclear factor (NF)-κB. Taken together, our data suggest that KAHF has a beneficial effect on several eicosanoid-related skin inflammations, such as atopic dermatitis.
Viral IL-10 Gene Transfer Prolongs Rat Islet Allograft Survival
Kim, Yang-Hee,Lim, Dong-Gyun,Wee, Yu-Mee,Kim, Jin-Hee,Yun, Chae-Ok,Choi, Monica-Y.,Park, Youn-Hee,Kim, Song-Cheol,Han, Duck-Jong SAGE Publications 2008 Cell transplantation Vol.17 No.6
<P>Islet transplantation is a potential cure for diabetes. However, allotransplant rejection severely limits its clinical application. In this study, we sought to transfect rat islets with an adenoviral vector containing the viral IL-10 (vIL-10) gene and examine its efficacy in preventing graft rejection. The immunosuppressive effect of vIL-10 is reported but its efficacy is somehow debatable in transplantation model. vIL-10 transfected islets were transplanted into streptozotocin-induced diabetic rats. Blood glucose, serum vIL-10 concentration, graft histology, and graft cytokine expression were used to monitor graft function up to day 21 after transplantation. Transfected islets released a large amount of vIL-10 protein without affecting their viability and functional integrity. When we transplanted the transfected islets into allogeneic hosts, the survival of grafted islets was not significantly increased. However, the combined use of vIL-10 and subtherapeutic doses of CsA (cyclosporine) significantly prolonged graft survival beyond that achieved with either agent alone (p < 0.001). vIL-10 and CsA-treated rats contain high level of vIL-10 in serum, which is evidenced by the inhibition of allogeneic mixed lymphocyte reaction (MLR). Histological analysis additionally revealed the presence of viable islets up to 21 days. IL-10 mRNA expression in grafted liver was higher and IFN-gamma mRNA was lower in vIL-10 and CsA-treated animals, compared with other groups. The synergistic effect of this combination therapy is potentially correlated with the induction of inhibitory cytokine secretion and downregulation of proinflammatory cytokine secretion from host cells.</P>
Jung, Hee-Yeon,Kim, Su-Hee,Seo, Min-Young,Cho, Sun-Young,Yang, Youngae,Choi, Ji-Young,Cho, Jang-Hee,Park, Sun-Hee,Kim, Yong-Lim,Kim, Hyung-Kee,Huh, Seung,Won, Dong Il,Kim, Chan-Duck The Korean Academy of Medical Sciences 2018 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.33 No.34
<P><B>Background</B></P><P>The association of de novo donor-specific anti-human leukocyte antigens (HLA) antibodies (DSA) and development of antibody-mediated rejection (AMR) in kidney transplant recipients (KTRs) is still undetermined.</P><P><B>Methods</B></P><P>We prospectively screened de novo DSA in 167 KTRs during 32 months after kidney transplantation (KT). Timing of DSA detection was at 3, 6, and 12 months post-transplant and annually thereafter and when clinically indicated. DSA levels were determined by Luminex assays and expressed as mean fluorescence intensity (MFI). We evaluated the incidence, characteristics of DSA, and association between DSA and tacrolimus trough levels or AMR.</P><P><B>Results</B></P><P>De novo DSA developed in 16 KTRs (9.6%) and acute AMR occurred more commonly in KTRs with de novo DSA compared to KTRs without de novo DSA (18.8% vs. 0%, <I>P</I> < 0.001). All de novo DSA were against class II antigens. The mean number of DSA was 1.8 ± 1.2 and the average MFI of DSA was 7,399 ± 5,470. Tacrolimus trough level during the first 0–2 months after KT was an independent predictor of DSA development (hazard ratio, 0.70; 95% confidence interval, 0.50–0.99; <I>P</I> = 0.043). No differences were found in the number of DSA, average MFI of DSA, and tacrolimus levels during the first year between de novo DSA-positive KTRs with AMR and those without AMR.</P><P><B>Conclusion</B></P><P>The results of our study suggest that monitoring of DSA and maintaining proper tacrolimus levels are essential to prevent AMR during the initial period after KT.</P>
Cho, Jang-Hee,Jang, Hye Min,Jung, Hee-Yeon,Choi, Ji-Young,Park, Sun-Hee,Kim, Chan-Duck,Yang, Chul Woo,Jin, Dong-Chan,Kim, Yong-Lim Elsevier 2018 Clinical therapeutics Vol.40 No.1
<P><B>Abstract</B></P> <P><B>Purpose</B></P> <P>Sevelamer, a noncalcium phosphate binder, has been shown to attenuate the progression of vascular calcification and improve survival in patients with chronic kidney disease undergoing dialysis compared with calcium-based binders. Using real-world data from a cohort study and the Health Insurance Review and Assessment Service database, we conducted a cost-effectiveness analysis comparing sevelamer with calcium acetate in dialysis patients from the perspective of the National Health Insurance Service in South Korea.</P> <P><B>Methods</B></P> <P>Data (demographic, diagnostic, laboratory, and survival) from 4674 patients undergoing dialysis enrolled in a multicenter prospective cohort study conducted in South Korea between September 2008 and December 2012 were linked to phosphate binder use, hospitalization, and cost data available from the Health Insurance Review and Assessment Service database. After propensity score matching, a dataset comprising comparable patients treated with either sevelamer (n = 501) or calcium acetate (n = 501) was used in the cost-effectiveness analysis. A Markov model was used to estimate costs, life years, quality-adjusted life years (QALYs), and cost-effectiveness over each patient’s lifetime. Forty-month treatment-specific overall survival (OS) data available from the dataset were extrapolated to lifetime survival with the use of regression analysis.</P> <P><B>Findings</B></P> <P>Patients had a mean age of 56.3 years and were treated with dialysis for a mean duration of 67.6 months. Compared with calcium acetate, sevelamer was associated with an incremental cost of South Korean Won (₩) 12,246,911 ($10,819) and a gain of 1.758 life years and 1.108 QALYs per patient. This outcome yielded incremental cost-effectiveness ratios of ₩6,966,350 ($6154) and ₩11,057,699 ($9768) per life year and QALY gained, respectively. Conclusions regarding sevelamer’s cost-effectiveness were insensitive to alternative assumptions in time horizon, discount rate, hospitalization rate, costs, and health utility estimates, and they remained consistent in 100% of the model iterations, considering a willingness-to-pay threshold of ₩31,894,720 ($28,176) per QALY gained.</P> <P><B>Implications</B></P> <P>This analysis of real-world data found that sevelamer’s higher cost relative to calcium acetate was adequately offset by improved survival among patients undergoing dialysis in South Korea. As such, sevelamer offers good value for money, representing a cost-effective alternative to calcium-based binders.</P>