Clinical relevance of combined anti-mitochondrial M2 detection assays for primary biliary cirrhosis

Han, Eunhee,Jo, Sung Jin,Lee, Hyeyoung,Choi, Ae-Ran,Lim, Jihyang,Jung, Eun-Sun,Oh, Eun-Jee Elsevier 2017 Clinica chimica acta Vol.464 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Antimitochondrial antibody (AMA) is a specific serologic marker in primary biliary cirrhosis (PBC). The aim of this study was to evaluate the clinical relevance of combined AMA assays.</P> <P><B>Methods</B></P> <P>Sera were obtained from 79 patients with PBC and 108 patients with other liver disease. They were tested by indirect immunofluorescence (IIF) using rat kidney/stomach tissue and HEp2 cells as substrate, 4 AMA-M2 assays, anti-sp100, and anti-gp210 assays.</P> <P><B>Results</B></P> <P>Using IIF-AMA with cut-off titer of 1:40, the sensitivity and specificity for PBC were 88.6% and 87.0%, respectively. A cut-off titer of 1:80 improved the specificity to 93.5%. The 4 commercial assay kits using AMA-M2 autoantibodies showed sensitivity of 55.7–79.7% and specificity of 91.7–95.4% with moderate to good agreement. AMA-M2 assays using both native and recombinant E2 antigens had higher sensitivity. ANAs on HEp2 cells, anti-sp100, and anti-gp210 were detected in 67.1%, 13.9–15.2%, and 22.8–27.8% of PBC patients, respectively. Additional AMA-M2 specific assays in IIF-AMA negative and low titer positive (1:40) sera increased the sensitivity and specificity to 88.6% and 90.7%, respectively.</P> <P><B>Conclusions</B></P> <P>Serological diagnosis for PBC using IIF with high titer cut-off and additional AMA-M2 specific tests by ELISA or LIA in IIF-negative sera should be used.</P> <P><B>Highlights</B></P> <P> <UL> <LI> IIF with cut-off value of 1:80 has high specificity for AMA detection. </LI> <LI> Assays using recombinant E3 antigens had high sensitivity for PBC diagnosis. </LI> <LI> IIF with high titer cut-off and additional M2 specific tests in IIF-negative sera should be used for PBC diagnosis. </LI> </UL> </P>

University Students’ Experience with MOOC for L2 Learning: A Case Study

( Han Eunhee ) 한국현대영어영문학회 2019 현대영어영문학 Vol.63 No.1

Because of the increasing popularity of the massive open online courses (MOOC), L2 learners are consequently being exposed to the MOOC. The purpose of this study was to investigate the L2 learner’s experience with MOOC. In this exploratory case there were twelve participants where interviews and observations were conducted, and the data from learner’s experiences with MOOC were gathered. Findings show that the L2 learners experienced confidence in language learning, possibly due to the connected content throughout a MOOC lecture, engaging in learning anytime and anywhere that they could access the internet. Also the university L2 learners perceived the MOOC as a new learning tool compared to that of their previous learning environment. The study findings imply that the MOOC may a useful tool to teach and learn L2 for university students. Further research and the study limitation are provided. (Korea Nazarene University)

A NEST’s Narrative: Teaching English in Korea between the Mid 1970s and the Early 2000s

Eunhee Han 한국영어교과교육학회 2019 영어교과교육 Vol.18 No.3

This study explores a qualitative evaluation of a NEST’s narrative of teaching experience from the mid-1970s to the early 2000s with Korean students. Narratives from one teacher who taught English in Korea were collected and analyzed. Data was gathered by oral and written narratives in the form of qualitative research. Narrative analysis was adapted, and events were analyzed inductively. Findings show that each decade has its uniqueness in terms of what teaching approaches applied, what roles a teacher and students had, how the class proceeded including the teacher’s reflections. In the 1970s, it was an imitation stage of teaching from other teachers with more of a teacher-oriented class. The next decade showed the changes of the teacher’s attitude and belief. The class became learner-oriented and created an enjoyable learning environment. During the 1990s an adjustment to the new teaching environment was required as the lab and co-teaching were introduced. The last decade of teaching brought a challenge to deal with computer usage in the class which also met with students being distracted by the computer. Study implications are provided with further study suggestion.

Research and Practice in Learning Portfolio Adoption on College Writing: A Case Study

Eunhee Han 한국외국어교육학회 2012 Foreign languages education Vol.19 No.4

The purpose of this study is to report and share an adoption on using learning portfolios in a university setting and writing through portfolio writing samples including personal drafts, peer feedback, teacher feedback, and personal reflections. By adopting the idea of using Learning Portfolio system, this study tries to report that it can be an applicable teaching/learning tool for English language learners (ELLs) in an English as a Foreign Language (EFL) context to facilitate their writing skills through using portfolio system. Participants were given a Learning Portfolio workshop which indicated how to make an appropriate portfolio and asked to collect their writing drafts, peer feedback, teacher feedback, and personal reflections. Both qualitative and interpretive analyses were conducted for 70 students’ portfolio samples throughout two different semesters. The study revealed that the students’ writing anxiety was reduced as they can check their progress through the collection of their portfolio. Errors of basic sentence structure, subject-verb relationship, and the tense were noticed by the peer feedback and teacher feedback. This study suggests that educators could be aware of, and consider adopting, the Learning Portfolio system in writing class so as to facilitate students’ writing development.

Characteristics of hematologic malignancies with coexisting t(9;22) and inv(16) chromosomal abnormalities

Han, Eunhee,Lee, Hyeyoung,Kim, Myungshin,Kim, Yonggoo,Han, Kyungja,Lee, Sung-Eun,Kim, Hee-Je,Kim, Dong-Wook Korean Society of Hematology; Korean Society of Bl 2014 Blood Research Vol.49 No.1

<P><B>Background</B></P><P>The coexistence of t(9;22)(q34;q11.2) and inv(16)(p13q22) chromosomal abnormalities is extremely uncommon, and only a small number of such cases have been reported. Here, we characterized 7 cases of hematologic malignancy exhibiting t(9;22) and inv(16) coexistence.</P><P><B>Methods</B></P><P>We reviewed the cytogenetic data for hematologic malignancies treated at the Catholic Blood and Marrow Transplantation Center between January 2004 and June 2013. We identified 7 cases exhibiting t(9;22) and inv(16) coexistence. In addition, we analyzed mutations in the <I>IKZF1</I>, <I>NPM1</I>, <I>FLT3</I>, <I>N-RAS</I>, <I>K-RAS</I>, <I>c-KIT</I>, and <I>TP53</I> genes.</P><P><B>Results</B></P><P>Four cases of chronic myelogenous leukemia (CML; 1 chronic phase, 2 accelerated phase, and 1 blast phase) and 3 cases of acute myeloid leukemia (AML; 1 de novo and 2 therapy-related) were identified. The percentages of circulating blasts and bone marrow eosinophils were higher in AML cases than in CML cases (53% vs. 5% and 30% vs. 5.5%, respectively). The proportions of each chromosomal abnormality were used along with follow-up karyotyping results to identify secondary changes. In <I>BCR/ABL</I>, a p210 fusion transcript was associated with CML, whereas a p190 fusion transcript was associated with AML. One patient with AML harbored 2 mutations: <I>c-KIT</I> D816V and <I>TP53</I> E11Q. All patients except 1 with CML blast phase sustained clinical remission after treatment, which included an imatinib mesylate regimen.</P><P><B>Conclusion</B></P><P>This study shows that observations of bone marrow morphology, initial and follow-up cytogenetic studies, and karyotyping of <I>BCR/ABL1</I> and <I>CBFB/MYH11</I> provide valuable information for characterizing hematologic malignancies exhibiting t(9;22) and inv(16) coexistence.</P>

Motivation

Eunhee Han 한국영어교과교육학회 2010 영어교과교육 Vol.9 No.3

This study examined the motivation of EFL students’ as they participate in a self-activity outside of the classroom curriculum through a self evaluation. Specifically, the researcher used the medium of English language television viewing to ascertain the effects of both integrative and instrumental motivation upon L2 learning. The study participants were 23 students including graduate, undergraduate, and English language program students in the U.S. The length of residence in America ranged from less than 1 year to 11 years. The major finding of this study was that both integrative and instrumental motivations are not determined by either the amount of time watching English language television or the length of residency in America. The implication of this study is that watching television programs in English outside of the classroom curriculum does promote learners’ interest and increased either or both their integrate and instrumental motivation. The need of more evidence from replicated studies with different groups of participants is also required.

Teaching EFL college writing

Eunhee Han 한국영어교과교육학회 2009 영어교과교육 Vol.8 No.2

The purpose of this study is to explore the experiences of EFL college students by providing a writing environment with a ‘grammatically- correctness-free-setting’ in the beginning stage of the writing process. The participants in this study comprise college students who enrolled in an English composition class in a university setting during the fall of 2009. The 31 students participated in this study. The participants in this study indicated that they perceived a non-threatening or little obsession with grammar correctness during the first two stages of the writing process which gave them freedom to write in English. Moreover, providing a non-threatening EFL writing class in the beginning stage of the writing process has potential to provide opportunities for college students to experience positive involvement in their writing, encouraging them to continue writing in English. The data analysis suggests that the increased freedom to use English writing skills may spring from providing two conditions in the classroom environment: (a) clear writing process instruction; and (b) a ‘grammatically-correctness-free-setting’ in the beginning writing stages. Further research are recommended.

The NNEST's Classroom English : Familiarity with Colloquial Language and Vocabulary Use

Eunhee Han(한은희) 새한영어영문학회 2008 새한영어영문학회 학술발표회 논문집 Vol.2008 No.10

The Role of Progymnasmata in English Writing Class

( Eunhee Han ) 한국중등영어교육학회 2014 중등영어교육 Vol.7 No.4

This paper aims to suggest an English writing class lesson in therhetorical approach method through using progymnasmata activity. Thebackground of study is from ancient rhetorical writing exercises especiallyfor beginner writers. Specifically it focuses on imitation and paraphrasingas a writing activity variation. The approach of study is to provide awriting activity through imitation and paraphrasing which can be viewed asa learning style and structure. The current teaching of the writingcommunity has hardly addressed the rhetorical approach in teachingwriting. From the rhetorical approach, the study questions the role ofprogymnasmata (which means elementary exercises) and its effectivenessin writing class. As a sequenced writing exercise, that begins withimitation and/or a simple paraphrase and ends with complicated and/orsophisticated exercises, the author hypothesizes that progymnasmata canbe applied to a writing activity. In an effort to suggest a writing activitythe author introduces one progymnasmata, fables, to be integrated into thewriting exercises and used to cultivate mental activity. The author showswriting lesson activities of how to imitate and paraphrase the originalfables. Finally, the pedagogical implication and its challenges aresuggested and brought into teaching the writing class.

사회보장정보시스템 행정데이터 활용 현황과 과제: 가명정보 결합 및 이용을 중심으로

한은희 ( Han Eunhee ) 한국보건사회연구원 2023 보건복지포럼 Vol.325 No.-

2020년 1월 개정된 개인정보보호법을 근거로 사회보장정보시스템에 축적된 행정데이터를 가명 처리하여 통계 작성, 과학적 연구, 공익적 기록 보존을 위해 이용하는 것이 가능해졌다. 법적 기반이 마련되었음에도 가명정보 결합 및 이용 절차의 모호성 및 복잡성으로 인해 사회보장정보시스템 행정데이터 활용은 다소 미흡한 실정이다. 이 글에서는 사회보장정보시스템 가명정보 이용 절차 및 가명정보 결합사례를 검토하고, 가명정보 활용을 활성화하기 위한 과제에 대해 논하였다. 사회보장정보시스템에서 제공 가능한 데이터 목록 및 메타데이터 제공, 사회보장정보시스템 행정데이터에 대한 이해 및 활용 능력 함양, 보존(retention) 가능한 통합데이터 셋 구축 등이 추진되어야 할 것이다. 무엇보다 사회보장 정보시스템 행정데이터를 활용한 정책연구의 결과를 반영할 수 있는 증거 기반 정책 평가 및 정책 설계쳬계가 확립되어야 한다. The Personal Information Protection Act, as amended in January 2020, allows through pseudonymization for the use of administrative data stored on the social security information system for statistical records, scientific research, and public interest purposes. Despite the legal framework in place, however, the use of administrative data remains limited to an extent due to the unclear and complicated procedures for combining and using pseudonymized data. This article examines specific examples of how pseudonymized social security administrative data has been used and combined and discusses what should be done to further the use of pseudonymized data. This study points out the need for creating a complete list of all the data that can be obtained from the social security information system and providing metadata for these data sets, enhancing people’s understanding of and capacity for using social security administrative data, and establishing an integrated dataset that allows for information retention. Most important of all is to establish an evidence-based system of policy evaluation and planning that takes account of the findings of policy research using social security administrative data.