Lipopolysaccharide로 유발된 생쥐 무릎 관절염 완화에 미치는 진통산의 효과 : 윤활관절막과 섬유관절막의 형태 변화를 중심으로 Based on the Morphological Change of Synovial Membrane and Fibrous Membrane

김진택,최홍식,안상현,박인식,강윤호,김호현,이해풍 동국대학교 경주대학 1999 東國論集 Vol.18 No.-

Knee joint BALB/C mice were administered with JINTONGSAN(JTS) extract after Lipopolysaccaride(LPS) injection were observed to investigate the anti-inflammatiory effect of JTS for arthritis. The JTS extract(3.3 ㎖/㎏/day) were daily administered to mice suffered from arthritis of knee joint induced by LPS injection, as dose of 300㎖/㎏. The specimen were fixed in 4% paraformaldehyde and were decalcificate in EDTA solution for 4 Weeks. In synovial membrane, the hyperplasia of synovial lining cell(fibroblast like synoviocytes) on JTS group were diminished then LPS group. The number of synovial lining cell(synovial phagocytic cells) with filopodia and the infiltrated of lymphocyte on JTS group were decreased. In fibrous membrane, The region of fibrosis on JTS group were mitigated than LPS group and the appearance of fibroblast and migrated cell, as neutrophil leukocytes and lymphocyte, were decreased. As results indicated that JTS was effective in anti-inflammatory reaction for LPS-induced arthritis.

들깨(Perilla frutescens) 잎과 줄기에서의 정유특성과 안정성

金海洙,金鐘熙 慶南大學校 附設 基礎科學硏究所 2000 硏究論文集 Vol.14 No.-

5개의 다른 장소에서 경작된 들깨를 대상으로 생잎과 줄기를 채취하여, Gas Chromatography Mass spectrometer(GC-MS)를 이용하여, 잎과 줄기에 함유된 정유의 조성과 함량을 분석하였다. 잎과 줄기의 정유의 조성은 매우 단순하였고(주요 성분은 8종류), 총함량은 건중의 1%정도였고, 그중 2-butanoyl furan 한 성분이 90%이상을 차지하였다. 줄기의 정유조성은 잎과 유사하나 함량은 잎의 1%정도에 불과하였다. 정유 총 함량은 계절에 따라 뚜렷한 차이가 있었고 7월이 가장 높았다. 각각 다른 온도에서 한시간 건조한 들깨잎은 여전히 30℃, 50℃에서는 약 90%, 80℃는 60%, 100℃ 40%이상의 정유를 함유하였고, 염수(salt solution)처리 6개월후의 들깨잎은 염농도와 관계없이 90%이상의 정유를 함유하고 있었다. The composition of essential oil in Perilla frutescens leaves and stems were identified and measured quantitatively by using Gas Chromatography and Mass Spetrometer (GC-MS). The total amount of essential oil in the leaf was much high, the highest total amount exhibited in July and varied with season, but the essential oil composition was much simple(the major composition was only eight kinds) and only 2-butanoyl furan composed over 90% of the total amount. The total amount in stem was very low and the composition also was very simple (only four kinds). After treatment with different temperature.(30℃, 50℃ 80℃ and 100℃), Perilla frutescens leaf still remained much of oil(at 30℃, 50℃ remained 90℃, 80℃ remained 60%, at 100℃ remained 40%) and after treatment with different concentration of salt solution (10%, 30%, 50% 80%)six months, it still remained over 90% of essential oil in fresh leaf.

人蔘正果가 Rat의 成長과 體成分에 미치는 影響

金乙祥,鄭鎭壹,金海中,金美正 단국대학교 1990 論文集 Vol.24 No.-

This study was carried out to investigate the effect of Honeyed ginseng(H.G.) on growth and biochemical components of rats. Grouping of rats in experiment was four different dietary groups as 0% H.G. group(control group), 1% H.G. group. 5% H.G. group and 10% H.G. group in continous ad libitum feeding for 3 weeks, repectively. Body weight gain of 1% H.G. group was more increased in comparison to control group. 5% and 10% H.G. groups. Liver weight of 1% H.G. group was more increased in comparison to others. But, spleen weight of 10% H.G. group was significantly decreased in comparison to control and 1% H.G. group(P<0.005). In biochemical component of plasma, total protein of 1% H.G. group was more increased in comparison to others. β-lipoprotein in plasma was significantly decreased in 1% and 5% H.G. group(P<0.05) and HDL-cholesterol was more increased in all H.G. groups. however, 5% H.G. group was significantly increased (P<0.05). Liver lipid of 10% H.G. group was more decreased in comparison to other groups. From the above result it was concluded that group treated with H.G. showed good nutritional efficiency in the body, especially, decreased a β-lipoprotein but increased HDL-cholesterol in plasma. 1% and 5% H.G. content of diet was superior to that of 10% in nutritional efficiency.

혈부축어탕의 고지혈증 유발 생쥐 신장내 지방 축적 감소효과

김호현,강윤호,방혜정,김진택,박인식,안상현,이해풍 世明대학교 한의학연구소 1998 韓醫學硏究所 論文集 Vol.1 No.-

Renal tissues of ICR mouse were intraperitoneally injected with Hyulboochucketang(HCE;1.28g/kg/day) after Triton WR-1339(TX;600mg/kg) injection were observed to investigate the lipid accumulation suppressive effect of HCE. The renal tissues were obtained at hour 24,48 and 72 after TX injection that were fixed in fromol-calcium solution and were cryocut. The tissue stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone method for cholesterol. The parietal layer of Bowman's capsule is swelled as cuboidal type at hour 48 after TX injection and the lipid blot and cholesterol particle were noticeably increased in glomerulus than control group. But the parietal layer of Bowman's capsule in HCE treated group was shaped as squamous type that was looked like as normal morphology and the lipid blot and cholesterol particle w3ere considerably decreased in glomerulus than TX group. As results indicated that the accumulation of lipid including cholesterol caused by TX injection were mitigated in renal tissues by the antihyperlipidermic effect of HCE.

들깨의 수용성화합물질과 그들의 타감작용

金海洙,金鐘熙 慶南大學校 附設 基礎科學硏究所 2000 硏究論文集 Vol.14 No.-

생잎과 뿌리 수용추출물이 함유하고 있는 화합물질은 phenolic compounds가 주였고, 잎추출물은 17종 acids와 9종의 비acids화합물질, 뿌리추출물에서는 15종의 acids와 6종의 비acids가 검정 및 동정되었다. 잎과 뿌리의 수용 추출물은 선정된 수용체 식물의 발아를 억제하였으며, 추출액 농도가 높을수록 억제 효과도 컸다. 잎의 경우 발아억제 효과는 고추, 쇠무름, 달맞이꽃 순 이었고, 뿌리는 쇄무름, 달맞이꽃, 고추, 상추 순이었다. 잎 추출물 억제효과는 뿌리추출물 보다 상대적으로 높았다. 그러나 오이, 배추, 무는 잎과 뿌리추출물 모두에서 억제효과가 현저히 낮지는 않았다. 잎과 뿌리의 수용 추출물은 선정된 수용체 식물의 성장에 상당한 억제효과를 일으켰다. 수용추출의 농도증가에 따라 억제효과가 현저히 증가되었으며, 잎의 경우 억제효과가 상대적으로 낮았다. 억제 효과는 잎과 뿌리모두에서 고추, 달맞이, 쇠무름이 낮았으며, 오이에 대한 억제효과가 가장 낮았다. 또한 잎과 뿌리수용추출물의 발아 및 성장 억제효과에서 야생종 쇠무름, 달맞이꽃에서 총체적으로 억제효과가 경작종 보다 높음을 보여주었다. The main compounds in aqueous extract of P. frutescens leaves and roots were the phenolic compounds. 17 acids and 9 non-acids compounds in leaves, 15 acids, and 6 non acids compounds in roots were identified. Aqueous extract of P. frutescens leaf and root had an inhibition effect on the germination of selected receptor plant species. The inhibition effects were higher along with the concentration raising, and inhibition effect from leaf extracts was much higher than in the root extracts. The leaf-extract inhibition effects on Capsicum annuum linne. Achyrant japonica. Oenothera odorta leaf extracts, and root-extract inhibition effects on Achyrant japonica. Oenothera odorta, Capsicum annuum. Lactuca sativa were much higher than others. Aqueous extract of P. frutescens leaves and roots also had inhibition effects on the elongation of selected receptor-plant species. The inhibition effects were represented higher along with the concentration raising, and effects on Capsicum annuum. Achyrant japonica. Oenothera odorta were much higher than others.

Lipopolysaccharide 로 유발된 생쥐 무릎관절낭 염증에 관한 형태학적 연구 : 윤활관절막과 섬유관절막의 변화를 중심으로 Based on the Morphological Changes of Synovial Membrane and Fibrous Membrane

김진택,안상현,최난희,정재만,박인식,강윤호,김호현,이해풍 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

본 연구는 관절염 유발시 일어나는 관절낭의 형태학적 변화를 조사하기위해 ljpopolysaccharide(LPS)주사로 인위적 관절낭 염증을 유발시킨 후 시간경과에 따른 윤활관절막과 섬유관절막의 형태 변화를 관찰하였다. BALB/C 암컷 생쥐 오른쪽 무릎관절낭에 LPS 300㎍/㎏를 주사한 후 3, 7 그리고 14일에 무릎관절을 얻었다. 무릎관절은 4주동안 EDTA용액에 탈회한 후 통상적 방법으로 paraffin에 포매하였다. 또한 윤활관절막의 미세구조변화는 embed812로 포매한 후 관찰하였다. LPS 주사후 관절연골 인접부위의 윤활관절막에서 시작된 세포과형성(hyperplasia)은 시간 경과후 전체 윤활관절막으로 확대되었다. 윤활관절막내의 미세구조의 변화로는 윤활포식세포(type 1)가 관절강내로의 많은 돌기(filopodia)를 내었고, 잘 발달된 과립형질내세망을 가지는 type 2 윤활분비세포의 숫적 증가가 보였다. 한편 LPS 주사후 섬유관절막에서 나타나는 형태학적 변화는 collagen fiber 생성에 의한 섬유화가 증가되며, 이러한 섬유화를 주도하는 섬유모세포의 이주증가파 관찰되었다. 또한 혈관 주위에서는 백혈구의 이주 증가가 나타났으며, 탈과립형(degranulated type) 비만세포가 많이 관찰되었다. 이상의 결과로 LPS 주사로 관절낭에서 염증이 유발되어 윤활관전막과 섬유관절막에서 형태학적 변화가 나타났다. 이러한 일련의 형태학적 변화는 발병초기 류마티스성 관절염에서 나타나는 병리학적 소건과 동일한 결과로서, 앞으로 진행될 치료제 개발과 유발기전에 관한 해석을 위한 in vivo 실험의 적절한 모델로 기여한 것으로 기대된다. Synovial joint of BALB/C mice were injeced with Lipopolysaccharide(LPS) were observed to investigate the morphological changes of synovial capsule caused by rheumatoid arthritis(RA). The RA on female Balb/c mice were induced by LPS injection, as dose of 300㎍/㎏, into synovial cavity of knee joint. And then these specimen were fixed in 10% neutral buffered formalin and were decalcificated in EDTA solution for 4 weeks. The hyperplasia of synovium were appeared in synovial membrane. The filopodia of phagocytic like synoviocyte(type Ⅰ synoviocyte) projected into synovial cavity and the number of fibroblast like synoviocyte(type Ⅱ synoviocyte) with well-developed endoplasmic reticulum were increased in synovium. In fibrous membrane, the fibrosis induced by synthesis of collagen fiber were enlarged to all fibrous membrane, and the number of fibroblast were increased. A great number of inflammation component cell as Iymphocyte and neutrophil leukocyte were infiltrated around capillary and the degranulate typed mast cell were increased. As results indicated that the hyperplasia of synovium induced by LPS, subsequently to cause the fibrosis, infiltration of imflammation component cell, and increase of degranulated type mast call as same as symptoms of RA.

중합효소연쇄반응을 이용한 도말양성 폐결핵 환자에서의 결핵균 DNA 검출방법에 관한 연구

이종진,김애경,조해정,한표성,홍석철,김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

Polymerase chain reaction (PCR) is the most sensitive and rapid method for the diagnosis of M.tuberculosis infection. Many reports have shown different methods for mycobacterial DNA extraction, but revealed many differences in simplicity and time-consumption. We studied which method was the best among 7 different ones for DNA extraction from 32 smear-positive sputa, using PCR of targeting 123bp DNA fragment of IS6110. The methods were the following : SDS-Microwave oven method, NaOH Lysis method, Triton X-100, Proteinase K method, Lysis buffer method. SDS-Proteinase K method, Bead Beater method, and Bead Beater/CTAB method. The amplification products were identified by electrophoresis on ethidium bromide-stained agarose gels, and confirmed by restriction enzyme, Southern blot hybridization, and DNA sequencing. All 32 cases were positive (100%) by the 7 different methods. It took 1 hour or more to detect DNA in SDS-Microwave oven method, NaOH Lysis method, and Bead beater/CTAB method. It took 2 hours or less in the others. SDS-Proteinase K method and Bead Beater/CTAB method were simpler and more convinient than the others. These results suggest that SDS-Proteinase K method and Bead Beater/CTAB method might be better than the others for the extraction of mycobacterial DNA in terms of simplicity and time-consumption, although all the methods were sensitive.

디자인경영의 유형별 전략모델에 관한 연구

이해묵,황세민,김규진 경기대학교 부설 산업디자인연구소 2010 조형논총 Vol.- No.7

메기 Parasilurus asotus L. 췌장의 췌도세포에 관한 세포화학적 연구

李海浜,金鎭澤 동국대학교 1986 論文集 Vol.25 No.-

Pancreatic islet cells of Catfish Parasilurus asotus L. were observed under light microscope. The results obtained were as follows; 1. the pancreas of Catfish consists of thronging Parenchymal tissue and pancreatic islets were located in the exocrine portion of the pancreas. 2. The pancreatic islets were surrounded by distinctive connective tissue capsul with the same morphological characteristics as principal islets of other teleosts. 3. The pancreatic islet was composed of B, A_1, A_2 cells. 4. AF-positive B cells were located in the central portion of the pancreatic islet and A_1, A_2 cells in the periphery of the pancreatic islet. 5. A_1 cells have the long cytoplasmic processes.

밤나무 혹벌의 寄生蜂인 남색꼬리좀벌 Torymus beneficus Y. 에 관한 硏究

李海浜,金鎭澤 동국대학교 농림과학연구소 1979 農林科學 論文集 Vol.5 No.-

From 1977 to 1978. the distribution and their parasitism of parasitoid Hymenoptera, Torymus beneficus Y. were studied from ten areas in Korea. The results are summarized as fallows; 1. Torymus beneficus Y. were distibuted in all studied areas. 2. Parasitism (%) of Torymus on Detocosmus had a big difference depend on collection sites from 65.96 % in Seoul to 0.4% in Kwangju. 3. On number of Dry ocosmus per gall, there were no difference between dense plantation area and disperse plantation of chestnut tree. but parasitism of Torymus were difference between two sites from 13.91 % in disperse plantation area to 40.53% in dense plantation area.