보육과 현장중심 교육과정 개발 : DACUM법을 중심으로 Based on the DACUM Method

김정신,노은호,이행숙,정해은,조희진 한국영유아보육학회 2004 한국영유아보육학 Vol.0 No.36

The quality of childhood educare teacher is the most important element in evaluation of the quality of childhood educare. However, the current curricula for the childhood educare in colleges are operated individually without a standard curriculum and there are many problems in the certification system of childhood educare teacher. So, it is hard to discuss about the specialty of the childhood educare teachers. Hence, an immediate development of a standard curriculum which fully reflect the needs of the field is necessary to meet the demand of the times and to enhance the quality of childhood educare service by producing qualified teachers. To fulfill the aforementioned needs, the procedure of the DACUM Method which is useful in developing vocational training course is used in this study. And based on the results, a field centered curriculum of the Dept. Child Care and Education is developed by the job analysis of the childhood educare teachers and by collecting the needs of the field. Educare planning and activities, development and evaluation of the educare program, consultation and education of the family, utilization of the regional resources, and after-class guidance of the children were found to be the principal jobs of the childhood educare teachers.

중증 화상 환자에서 영양지원

박지영,목혜숙,신현선,노순예,김애심,백금선,김도헌,허준,전욱 대한화상학회 2003 대한화상학회지 Vol.6 No.2

흰쥐 중추신경계내 난소로 투사하는 미주신경로에 관한 연구

김명주,장명세,고미희,노해숙,조해영,오문유,이봉희 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.1

본 실험은 부교감신경의 하나인 미주신경이 난소를 지배하는 신경으로 관여하고 있는지를 pseudorabies 바이러스를 이용하여 난소신경로와 난소주사후 미주신경절단을 통하여 조사한 연구 보고이다. 이를 위하여 Sprague Dawley계 암흰쥐를 대상으로 pseudorabies 바이러스를 난소에 주사한 무리와 난소 주사후 미주신경을 절단한 무리에서 뇌를 적출하여 pseudorabies 바이러스에 대한 면역조직화학 염색을 시행하여 비교하였다. 본 실험결과 미주신경 중추신경로내의 상위신경핵들이 pseudorabied 바이러스에 대하여 양성반응이 줄어들었거나 관찰되지 않는 차이를 보였다. 즉 적색핵, 종말판혈관기관, 줄무늬체, 침상핵과 이마엽겉질은 부분적으로 난소의 미주신경로에 관여하고 있으며 미주신경등쪽핵, 고립로핵, 최후영역, 청색반점, 팔옆핵, 코리케퓨즈핵, 흑색질 및 시각교차위핵은 양성반응이 관찰되지 않아 미주신경으로 투사하는 부교감신경핵으로 조사되었다. The mammalian ovary has been known as receiving its innervation by sympathetic and sensory neurons of the peripheral nervous system from the brain. Recently, there were several functional reports that the vagus nerves were also regulating the ovarian function, but the vagus nerve had not been identified by clear morphological evidence. A viral transneuronal tracing technique has been used to demonstrate the morphological evidence for the central vagal involvement in ovarian innervation in brain areas. Bartha strain of pseudorabies virus injection was made into the ovary of Sprague Dawley rats. In experimental group, the vagus nerve of the same injection side was removed right after ovarian injection. At five days after initial injection, all the rats were sacrificed and brains were processed for immunohistochemistry. Several central nuclei including hypothalamic paraventricular nucleus showed strong bilateral positive labelings after unilateral injection in control rats, but the positive labelings were disappeared or decreased in several hypothalamic nuclei and nuclei of the vagus nerve. I n conclusion, these results provide the morphological evidence that vagus nerve has neural connection to ovary and by which the central nervous system may maintains the state of ovulation and reproduction as a possible parasympathetic routes in mammals.

Ketogenic diet protects the hippocampus from kainic acid toxicity by inhibiting the dissociation of bad from 14-3-3

Noh, Hae Sook,Kim, Yoon Sook,Kim, Young Hee,Han, Jae Yoon,Park, Chang Hwan,Kang, Ahn Ki,Shin, Hee Suk,Kang, Sang Soo,Cho, Gyeong Jae,Choi, Wan Sung Wiley Subscription Services, Inc., A Wiley Company 2006 JOURNAL OF NEUROSCIENCE RESEARCH - Vol.84 No.8

<P>The ketogenic diet (KD) is often effective for intractable epilepsy, but its antiepileptic mechanisms remain largely unknown. Within the cell death/survival pathway, Akt and its downstream protein Bad play an important role in kainic acid (KA)-induced cell death. Therefore, we investigated the effects of a KD on KA-induced changes in the Akt/Bad/14-3-3 signaling pathway by evaluating Akt, Bad, 14-3-3, and cleaved caspase-3 expression levels as well as their relative interactions. Our results showed that a KD did not affect the expression levels of Akt, Bad, Bcl-xL, Bax, and 14-3-3 but increased phospho-Akt [serine 473; p-Akt (Ser473)] and phospho-Bad [serine 136; p-Bad (Ser136)] expression levels as well as decreased cleaved caspase-3 levels following a KA-induced seizure in the hippocampus. Furthermore, we found that a KD increased the protein–protein interaction between 14-3-3 and p-Bad (Ser136), which might be phosphorylated by p-Akt (Ser473), and decreased interaction of Bad and Bcl-xL. These results suggest that a KD might protect, at least partially, the hippocampus from KA-induced cell death via inhibiting the dissociation of Bad from 14-3-3. © 2006 Wiley-Liss, Inc.</P>

Acetoacetate protects neuronal cells from oxidative glutamate toxicity

Noh, Hae Sook,Hah, Young-Sool,Nilufar, Rashidova,Han, Jaehee,Bong, Jae-Hwan,Kang, Sang Soo,Cho, Gyeong Jae,Choi, Wan Sung Wiley Subscription Services, Inc., A Wiley Company 2006 JOURNAL OF NEUROSCIENCE RESEARCH - Vol.83 No.4

<P>Glutamate cytotoxicity contributes to neuronal degeneration in many central nervous system (CNS) diseases, such as epilepsy and ischemia. We previously reported that a high-fat and low-carbohydrate diet, the ketogenic diet (KD), protects against kainic acid-induced hippocampal cell death in mice. We hypothesized based on these findings that ketosis resulting from KD might inhibit glutamate cytotoxicity, resulting in inhibition of hippocampal neuronal cell death. Therefore, we investigated the role of ketone bodies [acetoacetate (AA) and β-hydroxybutyrate (β-OHB)] both in a mouse hippocampal cell line (HT22) and in rat primary hippocampal neurons. As a result, we found that pretreatment with 5 mM lithium AA and 4 mM Na β-OHB protected the HT22 hippocampal cell line and primary hippocampal neuronal culture against 5 mM glutamate toxicity and that up to 2 hr of pretreatment with 5 mM AA had a protective effect against 5 mM glutamate toxicity in the HT22 cell line. Pretreatment with 5 mM AA decreased ROS production of HT22 cell line at 2 and 8 hr exposure of glutamate, and it decreased the appearance of annexin V-positive HT22 cells, which are indicative of an early stage of apoptosis, and propidium iodide-positive HT22 cells, which are indicative of necrosis. © 2006 Wiley-Liss, Inc.</P>

Neuroprotective effects of the ketogenic diet

Noh, Hae Sook,Kim, Yoon Sook,Choi, Wan Sung Blackwell Publishing Inc 2008 Epilepsia Vol.49 No.suppl8

<P><SMALL>SUMMARY</SMALL></P><P><B>The ketogenic diet (KD) is an alternative treatment for medically refractory epilepsy. Despite numerous mechanistic hypotheses advanced to explain the anticonvulsant action of the KD, few studies to date have addressed the molecular changes in brain following KD treatment. Here, we present recent experimental results based on systemic administration of kainic acid (KA) in rodents. KA typically induces acute limbic seizures and results in cellular and molecular alterations, accompanied by neuronal death mainly in limbic structures, similar to what has been observed in surgically resected temporal lobe tissue in epileptic patients. We have reported that neuronal degeneration induced by KA is ameliorated by KD treatment via diverse protective mechanisms, including inhibition of caspase-3-mediated apoptosis in hippocampal neurons. Neuroprotective strategies such as the KD, if implemented early, might exert an antiepileptogenic effect, and could prevent associated learning and memory deficits.</B></P>

PEBP1, a RAF kinase inhibitory protein, negatively regulates starvation-induced autophagy by direct interaction with LC3

Noh, Hae Sook,Hah, Young-Sool,Zada, Sahib,Ha, Ji Hye,Sim, Gyujin,Hwang, Jin Seok,Lai, Trang Huyen,Nguyen, Huynh Quoc,Park, Jae-Yong,Kim, Hyun Joon,Byun, June-Ho,Hahm, Jong Ryeal,Kang, Kee Ryeon,Kim, D Informa UK (TaylorFrancis) 2016 AUTOPHAGY Vol.12 No.11

<P>Autophagy plays a critical role in maintaining cell homeostasis in response to various stressors through protein conjugation and activation of lysosome-dependent degradation. MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 ) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). In this study, we identified a new putative LIR motif in PEBP1/RKIP (phosphatidylethanolamine binding protein 1) that was originally isolated as a PE-binding protein and also a cellular inhibitor of MAPK/ERK signaling. PEBP1 was specifically bound to PE-unconjugated LC3 in cells, and mutation (WXXL mutated to AXXA) of this LIR motif disrupted its interaction with LC3 proteins. Interestingly, overexpression of PEBP1 significantly inhibited starvation-induced autophagy by activating the AKT and MTORC1 (mechanistic target of rapamycin [serine/threonine kinase] complex 1) signaling pathway and consequently suppressing the ULK1 (unc-51 like autophagy activating kinase 1) activity. In contrast, ablation of PEBP1 expression dramatically promoted the autophagic process under starvation conditions. Furthermore, PEBP1 lacking the LIR motif highly stimulated starvation-induced autophagy through the AKT-MTORC1-dependent pathway. PEBP1 phosphorylation at Ser153 caused dissociation of LC3 from the PEBP1-LC3 complex for autophagy induction. PEBP1-dependent suppression of autophagy was not associated with the MAPK pathway. These findings suggest that PEBP1 can act as a negative mediator in autophagy through stimulation of the AKT-MTORC1 pathway and direct interaction with LC3.</P>

Mitotic Cell Cycle Checkpoints(Cell Cycle and Cell Death) : Calpain-mediated cleavage of Beclin 1 is specifically associated with the oxidative stress-induced autophagic cell death

( Hae Sook Noh ),( Young Sool Hah ),( Deok Ryong Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2008 생화학분자생물학회 춘계학술발표논문집 Vol.2008 No.-

Propofol Protects the Autophagic Cell Death Induced by the Ischemia/Reperfusion Injury in Rats

Noh, Hae-Sook,Shin, Il-Woo,Ha, Ji-Hye,Hah, Young-Sool,Baek, Seon-Mi,Kim, Deok-Ryong Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.5

Autophagy has been implicated in cardiac cell death during ischemia/reperfusion (I/R). In this study we investigated how propofol, an antioxidant widely used for anesthesia, affects the autophagic cell death induced by the myocardial I/R injury. The infarction size in the myocardium was dramatically reduced in rats treated with propofol during I/R compared with untreated rats. A large number of autophagic vacuoles were observed in the cardiomyocytes of I/R-injured rats but rarely in I/R-injured rats treated with propofol. While LC3-II formation, an autophagy marker, was up-regulated in the I/R-injured myocardium, it was significantly down-regulated in the myocardial tissues of I/R-injured and propofol-treated rats. Moreover, propofol inhibited the I/R-induced expression of Beclin-1, and it accelerated phosphorylation of mTOR during I/R and Beclin-1/Bcl-2 interaction in cells, which indicates that it facilitates the inhibitory pathway of autophagy. These data suggest that propofol protects the autophagic cell death induced by the myocardial I/R injury.

세대에 따른 유아의 실개천 놀이 양상에 관한 문화기술적 연구

노혜진(Noh Hae Jin),최경숙(Choi Kyung-sook),김윤나(Kim Yunna),박미숙(Park Mi Sook),임지혜(Lim Jee Hye),박희숙(Park Hee-suk) 학습자중심교과교육학회 2020 학습자중심교과교육연구 Vol.20 No.4

본 연구는 세대에 따른 유아의 실개천 놀이문화 양상을 알아보는 데 그 목적이 있다. 이를 위하여 본 연구에서는 실개천이 있는 A마을에서 유년시절을 보낸 노인 5명과 실개천에 놀이하러 정기적으로 오는 유아 8명을 연구참여자로 선정하여 문화기술적 연구를 시행하였다. 2017년 1월부터 2018년 2월까지 참여관찰, 심층면담, 현장 메모 등으로 자료를 수집하였으며, 수집된 자료는 전사, 코딩, 영역분석과 주제분석의 과정을 거쳤다. 분석 결과, 노인들이 A마을 실개천에서 행한 어린 시절 놀이는 ‘고통을 뒤로 한 풍성함’, ‘아찔함과 함께한 즐거움’, ‘몸이 기억하는 감정’으로 나타났다. 그리고 현대 유아들의 실개천 놀이는 ‘두려움을 넘어선 생성’, ‘자연과의 교감’, ‘옛 세대와의 교감’으로 나타났다. 이를 바탕으로, 실개천을 중심으로 한 노인들의 어린 시절 놀이와 현대 유아들의 놀이 사이에는 공동체와 함께함, 몸으로 체화함, 자연물로 놀이함이라는 공통점을 발견하였다. 또한 실개천을 바라보는 관점과 실개천 내 생물을 바라보는 관점, 실개천에서 이루어지는 놀이에 영향을 주는 요인들에서 두 세대 간 차이가 있음을 발견하였다. 동일한 공간에서 이루어지는 놀이를 통시적으로 분석했다는 점에서 의의가 있는 본 연구는, 시대에 따른 유아 놀이 변화 연구의 기초자료가 될 것으로 사료된다. The purpose of this study is to explore children’s play around a stream from a diachronic view. For this reason, this ethnographic study selected five elderly who spent their childhood in A village with the stream, and eight children who come to play in the reconstructed stream regularly. This study was conducted from January, 2017 to February, 2018 through participant observation, in-depth interview, and field notes. The data was transcribed, coded, and categorized through domain analysis and theme analysis. The themes from this study are as follows: in the old people’s childhood, children’s play were characterized as ‘richness with pain’, ‘pleasure with dizziness’, and ‘emotions that the body remembers’. On the other hand, modern children’s play are characterized as ‘creation beyond fear’, ‘communication with nature’, and ‘communication with the old generation’. Based on this analysis, this study found common ground between children’s play around the stream of the elderly and that of the modern children: play with communal harmony, subjected with the body, and play with the nature. This study also found that there were differences between the two generations in terms of the view of the stream, the view of lives in the stream, and the factors that affected the play around the stream. It is believed that this study, which is meaningful in that it has analyzed the play in the same space with diachronic viewpoint, will be the basis for the study of children’s play changing over time.