인공사육장에서 패혈증으로 집단폐사한 북방산개구리의 증례 보고

정여진 ( Yeo Jin Jeong ),김종택 ( Jong Taek Kim ),서국현 ( Guk Hyun Suh ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.3

Frog culture industry is not yet familiar but has much potential. Generally, in farm, the population density is higher than that of in nature and frog farm is not the exception. But when population density is high, it can easily leads to stressful condition, poor sanitation. When a disease occur, it is a primary factor that makes the population more susceptible and the results more grave. Because of severe Rhabditoidea- helminth infection and subsequent bacterial septicemia, 50¡­70% of the total population had been died in a farm in Jeong-sun in Gangwon-do and Chungju in Chungcheongbuk-do from late June, 2012 to September, 2012. Diseased frogs showed ruptured lung, bloody ascites, liver discoloration, myocardium weakness, congested kidney, microcytic anemia and so on. Enterobacteriacea, Citrobacter.sp, Cupriavidus metallidurans, Acinetobacter.sp were isolated as major bacterium that had caused septicemia in frogs. Among isolated bacterium, Cupriavidus metallidurans, Ewingella americana, Shewanella aquimarina and Pseudoalteromonas sp. have not reported as potential pathogens in frogs before. It is a good example that severe helminth infection in frogs can lead to secondary infection of bacteria.

면역방사계수법을 이용한 Thyroglobulin 측정시 항 Thyroglobulin 항체의 존재가 미치는 영향

안병철,배진호,정신영,박호용,김정국,하승우,이재태,김보완,이규보 대한내분비학회 2004 Endocrinology and metabolism Vol.19 No.1

연구배경: 혈청 thyroglobulin (Tg)은 옥소전신스캔과 함께 분화갑상선암환자에서 갑상선암 재발에 대한 추적관찰에 매우 예민하고, 중요한 표지자로 임상에서 널리 이용된다. 그러나 많은 수의 분화갑상선암환자는 Tg 항체인 항 Tg 항체를 가지고 있고, 이는 방사면역검사법 (radioimmunoassay) 및 면역방사계수 검사법(immunoradiometric assay)으로 Tg 측정시 영향을 미칠 수 있다. 이에 연자들은 면역방사계수법으로 Tg를 측정할 경우, 항 Tg항체에 의하여 어떤 영향이 생길수 있는지를 알아보고자 하였다. 방법: 이중위치 고상법 (solid phase two-site)법을 이용하는 ELSA-hTg in vivo test (CIS international, Schering, France) 시약을 이용하여 Tg를 측정하였다. Tg과 항 Tg 항체는 검사시약에 포함된 표준용액을 사용하였으며, Tg는 두 가지 농도 (23.5 ng/mL, 62.5 ng/mL)로, 항 thyroglobulin 항체는 세 가지 농도(25U/mL, 50U/mL, 100U/mL)로 이용하였다. Tg가 높게 나타나는 환자의 혈청에 항 Tg 항체가 높게 나타나는 환자의 혈청을 혼합하여 동일한 방법으로 Tg 검사를 시행하였다. 통계학적 분석은 ANOVA와 Scheffe test 및 Quadratic regression modeling을 시행하였다. 결과: 면역방사계수법을 이용한 Tg 측정시 항 thyroglobulin 항체가 존재하는 경우 그 값이 낮게 측정되었다. Tg 농도가 23.5 ng/mL인 표준용액이 항 Tg 항체의 농도가 0, 25, 50 및 100U/mL 경우, 각각 24.5±1.1, 11.8±0.4, 7.7±0.1 및 4.5±0.4ng/mL로 측정되었으며, Tg 농도가 62.5 ng/mL인 표준용액은 각각 65.9±5.7, 36.3±2.2, 23.7±0.7, 및 14.0±1.0 ng/mL로 측정되었다(ANOVA test, p=0.000). 항 Tg 항체에 의한 Tg 측정값의 저하 정도는 항 Tg 항체의 농도에 비례하는 것으로 나타났다 (Quadratic model regression, SigT=0.000). 결론: 분화갑상선암환자에서 항 Tg 항체의 존재는 면역방사계수법을 이용한 혈청 Tg 측정으로 갑상선암 재발을 파악하는데 방해하는 인자로 작용될 수 있으며, Tg 측정시 항 Tg 항체의 측정이 꼭 필요하며, 항 Tg 항체를 가진 분화갑상선암환자에서 Tg 수치를 해석할 경우 세심한 주의가 요구된다. Background: Serum thyroglobulin (Tg) is a valuable and sensitive tool needed in the follow-up of patients with differentiated thyroid cancer (DTC), but antithyroglobulin antibody (Anti-Tg), common in patients with DTC, can interfere with the assay for Tg. In this study, we evaluated the influence of Anti-Tg on the measurement of Tg using the immunoradiometric assay (IRMA). Methods: In using ELSA-hTg in vivo test (CIS international, Schering, France), a solid phase two-site IRMA was used to measure Tg (23.5 ng/mL, 62.5 ng/mL) under the absence or presence of three concentrations of Anti-Tg (25U/mL, 50U/mL, 100U/mL). We also performed Tg measurement using patients serum that was mixed with patients serum containing high Anti-Tg. ANOVA and Scheffe tests were performed to evaluate the effect of Anti-Tg on Tg IRMA, and an inverse regression was made to calculate the level of Tg from measured Tg and used Anti-Tg levels and also to assess the degree of effect of anti-Tg on Tg IRMA. Results: In measuring Tg using the standard solution, the presence of Anti-Tg resulted in a falsely suppressed Tg value. The IRMAs for 23.5ng/mL of the standard Tg solution resulted in 24.5±1.1 ng/mL under no Anti-Tg, 11.8±0.4 ng/mL under 25U/mL of Anti-Tg, 7.7±0.1 ng/mL under 50U/mL of Anti-Tg, and 4.5±0.4 ng/mL under 100U/mL of Anti-Tg. IRMAs 62.5 ng/mL of the standard Tg solution resulted in 65.9±5.7 ng/mL under no Anti-Tg, 36.3±2.2 ng/mL under 25U/mL of Anti-Tg, 23.7±0.7 ng/mL under 50U/mL of Anti-Tg, and 14.0±1.0 ng/mL under 100U/mL of Anti-Tg. (ANOVA test, p=0.000). The degree of suppression of the measured Tg value was positively correlated with the Anti-Tg level (Quadratic model regression, Sig T=0.000). The presence of Anti-Tg also resulted in a falsely suppressed Tg value for the Tg measurement using patient's serum. 2004). Conclusion: The presence of Anti-Tg could consist of the use of Tg as a tumor, therefore Anti-Tg should be measured in all patients diagnosed with DTC. The interpretation of the Tg level must be performed with extreme caution in patients with Anti-Tg (J Kor Soc Endocrinol 19:42∼47, 2004)

기니이픽 장관의 c-Kit 및 NK 1R 면역반응 세포구조에 대한 공초점 주사현미경적 연구

장인엽,김종중,문정석,김현곤,박찬국,전제열,전규배,조철희,유호진 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Background and Objectives: Immunolabelling of interstitial Cajal(IC) cells in the intestinal wall has recently been developed by using a specific marker, the anti-c-Kit antibody. Substance-P is a well-known neurotransmitter in the gastro-intestinal tract. Since the gastro-intestinal wall structures have already been well documented in the guinea pig, immunohistochemistry was done for the c-Kit-positive IC network and substance-P receptor(NK1R) in an attempt to provide a morphological basis for the mechanism regulating gastro-intestinal movement. Materials and Methods: Cryosection and whole-mount preparations of guinea pig small intestine and colon were single and double immunolabelled using the anti-c-Kit and NK1R antibodies. Immunolabelled specimens were observed under a confocal laser scanning microscope. Results : According to a three dimensional reconstruction study, it was found that (1) the c-Kit-positive celluar networks were widely distributed in the intestinal wall, (2) c-Kit-positive celluar networks encircled the ganlion, with strands in reticular configurations, and (3) the c-Kit-positive cells showed colocalization with NK1R in circular muscle(CM), not myenteric plexus(MY). Conclusion: The charateristic profiles of IC containing c-Kit-positive celluar networks and the relationship between c-Kit-positive and NK1R-positive structures provide a morphological basis upon the mechanism regulating gastro-intestinal motility.

Lipedematous alopecia in an asian woman: is it an advanced stage of lipedematous scalp?

( Guk Jin Jeong ),( Ji Yeon Hong ),( Kapsok Li ),( Seong Jun Seo ),( Chang Kwun Hong ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Lipedematous alopecia (LA) is a rare disease entity that manifests as increased thickness of subcutaneous fatty tissue in the scalp with a variable degree of acquired, non-scarring alopecia. Although the pathogenesis of alopecia in LA is not clear, it is assumed that the increased thickness of subcutaneous fat retards hair growth by disturbing the peripheral microcirculation of the hair bulb. LA is clinically distinguishable from lipedematous scalp (LS) in that LS is not associated with any hair abnormalities, while LA is characterized by variable degree of hair loss. LS and LA show similar distribution in ages of onset around the fourth decade and in affected areas of the vertex and occiput. A 52-year-old woman presented to our clinic with a pink, shiny, dome-shaped nodule on her frontal scalp. Physical examination revealed sparse hair density and diffuse hair thinning, while histopathologic findings revealed a marked hyperplasia of the subcutaneous fat. Here, we describe a rare case of LA on the frontal scalp of an Asian woman. We suggest that LS and LA may coexist in a spectrum of lipomatous lesion of scalp with morphological variations.

A Refractory Filler Granuloma Successfully Treated with a Combination of an Insulated Monopolar Radiofrequency (RF) Microneedle Device and a Quantum Molecular (QM) Resonance Technology Device

Guk Jin Jeong,Jae Wan Park,Sun Hye Shin,Young Gue Koh,Seog Kyun Mun,Kwang Ho Yoo,Beom Joon Kim 대한의학레이저학회 2022 MEDICAL LASERS Vol.11 No.1

With an increase in the indications and number of filler injection cases, there has been an increase in the complications related to the treatment. A foreign body granuloma is a late/delayed-onset dermal filler complication. Despite its rare incidence, a filler granuloma can cause both cosmetic and psychological problems in patients, especially when it is chronic and refractory to treatment. We report a case of a patient with a chronic refractory filler granuloma that was successfully treated with a combination of an insulated monopolar radiofrequency microneedle device and a quantum molecular resonance technology-based device. We expect further developments in technology and medical devices for effective treatment of filler granulomas.

Brimonidine tartrate gel for the prevention of laser therapy-related postinflammatory hyperpigmentation

( Guk Jin Jeong ),( Hyun Jung Kwon ),( Kui Young Park ),( Hae Woong Lee ),( Beom Joon Kim ),( Myeung Nam Kim ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Various laser treatments are used to treat hyperpigmented lesions such as solar lentigo and chloasma. However, they often leave unwanted postinflammatory hyperpigmentation (PIH) especially in dark-skinned individuals. A topical gel of brimonidine is a highly selective α-2 adrenergic agonist approved for the treatment of rosacea due to its potent vasoconstrictive activity in cutaneous blood vessels. Two middle-aged man with Fitzpatrick skin type IV underwent Q-switched laser treatment in our clinic to remove solar lentigines from their face. Prior to and after laser treatment, topical brimonidine gel was applied to one of the lentigines. Brimonidine-applied lesions showed more significant improvement leaving less PIH than non-applied lesions. Herein, we suggest topical application of brimonidine could reduce PIH after Q-switched laser treatment of lentigine in a dark-skinned patient.

Pyoderma gangrenosum mistaken as chronic venous ulcer

( Guk Jin Jeong ),( Hyun Jung Kwon ),( Kui Young Park ),( Beom Jun Kim ),( Myeung Nam Kim ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Pyoderma gangrenosum (PG) is a destructive inflammatory disease characterized by painful ulcerative lesions with neutrophilic infiltration in the dermis. The etiology and pathogenesis of PG are poorly understood. Although approximately 50% of the cases on PG have been described in association with inflammatory bowel disease and other hematologic diseases, a few rare cases of PG have indicated ulcer development initiated only after minor trauma and ischemic condition. A 66-year-old man referred to our clinic presenting multiple painful ulcers on the both lower legs which had been relapsed for past 4 years. Pitting edema and surrounding brownish reticular pigmented patch were also found. He received total knee replacement surgery a year before those ulcers occurred. Before being referred to our clinic, he was diagnosed as venous leg ulcer by these clinical features and prior history of surgery. In spite of prolonged treatments for venous leg ulcers, the lesions had not been improved. Through the skin biopsy, PG was diagnosed and systemic corticosteroid treatment was added to the ongoing conventional treatment for venous leg ulcers. The recalcitrant ulcers responded well and showed a dramatic improvement. Here, we report an atypical case of PG on the site of venous insufficiency leading to an inevitable misdiagnosis as venous ulcers.

Chondroid syringoma of the lower leg: an overlooked diagnosis

( Guk Jin Jeong ),( Ji Yeon Hong ),( Kapsok Li ),( Seong Jun Seo ),( Chang Kwun Hong ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Chondroid syringoma (CS) is a rare, benign skin appendageal tumor with its typical histologic appearance showing nests of cuboidal or polygonal cells and ducts surrounded by chondromyxoid stroma. It is usually not recognized by physicians due to the relative infrequency and silent clinical course. CS usually presents in the head and neck area, except for rare cases involving the trunk, back, or extremities. A 54-year-old female patient presented with a three-year history of a protruding nodule on her right lower leg. She did not complain of any subjective symptoms, except aesthetically unpleasant brownish pigmentation appeared after squeezing it. Through skin biopsy, CS was confirmed and the patient underwent complete excision. Herein, we report a case of CS on an unusual location, which should be considered as a differential diagnosis of a solid tumor of extremities.

Panfolliculoma: an overlooked diagnosis

( Guk Jin Jeong ),( Jae Min Kim ),( Kui Young Park ),( Kapsok Li ),( Seong Jun Seo ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

Panfolliculoma is a rare, benign skin appendageal tumor with its distinctive histologic appearance showing differentiation towards all components of the hair follicle, including the upper and the lower segments. It usually presents as a solitary cystic tumor resembling other lesions, such as basal cell carcinoma and trichoepithelioma. A 69-year-old female presented with a five-year history of a protruding nodule on her left thigh. It has shown slow increase in growth without subjective symptoms. Through skin biopsy, panfolliculoma was finally diagnosed. Herein, we report the first case of panfolliculoma in Korean literature which should be considered as a differential diagnosis of benign appendageal tumor entitiy.

Efficacy and safety of botulinum toxin a injection in recalcitrant erythematotelangiectatic rosacea: a pilot study

( Guk Jin Jeong ),( Hyun Jung Kwon ),( Kui Young Park ),( Seong Jun Seo ),( Myeung Nam Kim ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: There are few pharmacologic options to reduce erythema and flushing in patients with recalcitrant erythematotelangiectatic rosacea (ETR). Development of a novel therapeutic approach to ETR is required. Objectives: This study aimed to investigate the efficacy and safety of intradermal botulinum toxin injection as an aid in persistent erythema of rosacea patients. Methods: A total of 20 Korean patients with recalcitrant ETR were enrolled to receive treatment by injection of botulinum toxin. Patients received one treatment of intradermal botulinum toxin injection and were assessed 1, 2, 4 and 8 weeks after treatment. The severity of erythema and telangiectasia was investigated by a non-treating physician, and the erythema index (EI) was assessed by mexameter at each visit. Patient's satisfaction and any adverse events were also assessed at each visit. Results: Intradermal injection of botulinum toxin significantly reduced erythema severity and EI in ETR patients. Patients reported a satisfaction score of 2.94 in mean 5-point scale at 8 weeks after treatment. Patients participating in the final analysis did not report side effects except injection pain and temporary facial muscle paralysis. Conclusion: Intradermal injection of botulinum toxin can be used as an effective and relatively safe adjuvant agent for recalcitrant and persistent erythema of ETR patients.