위암수술 환자에서의 cp 개발과 cp적용에 따른 질 향상 활동에 관한 연구

임은주,황순휘,박도중,하광일,배현주,김형호 한국의료QA학회 2006 한국의료질향상학회지 Vol.13 No.2

Background : In an era of increasing medical costs, safe reduction in postoperative stay has become a major focus to optimize utilization of healthcare resources. The authors aimed to evaluate the clinical significance of the critical pathway(CP) for gastrectomy patients by implementing standardized postoperative management and electronic medical records. Method : From August 2006 to April 2007, critical pathways were introduced to inpatients and outpatients with gastric cancer. 60 consecutive patients undergoing distal gastrectomy were randomly divided into two groups; 30 CP group(A) and 30 non-CP group(B). Simultaneously, we also retrospectively reviewed the records of 438 patients(C:control group) who were able to be enrolled in CP program. We compared group(A) with (B), (C) in terms of hospital stay, complication rate, use of antibiotics and hospital costs. Patient satisfaction was surveyed by questionnaires. Result : There was no significant demographic difference between group(A) and (B). Of 30 patients in group(A), 5 drop-outs and 14 variances occurred. The mean postoperative hospital stays(Laparoscopy-assisted distal gastrectomy: LADG/Open distal gastrectomy: ODG) were 7.6/10.5 days for the group(A), 8.9/12.7 days for the group(B) and 12.6/14.5 days for the group(C) (p=0.02 in LADG). The total hospital cost of group(A) had a tendency to be less than that of group(B) and the amount of hospital cost per day in group(A) was significantly higher than that in group(B). The usage rates of the first generation antibiotics were 73.33%, 63.33%, and 59.2% in group(A), (B), and (C), respectively. Patients’ satisfaction had a tendency to be higher in the CP group than non-CP group. Conclusion : The mean hospital stay in LADG patients was shortened meaningfully and hospital cost per day was enlarged after introduction of clinical pathway. It is necessary to apply a number of patients to the critical pathway and to make an effort to reduce the variation and drop-out rates.

Helicobacter pylori 항원을 이용한 면역우유 생산 및 항체특성에 관한 연구

배만종,김수정,예은주,김병기,박창호,김미경 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

본 연구는 위염, 위궤양, 위림프종 및 위암과 같은 소화기 질환의 원인균으로 알려진 Helicobacter pylori균을 항원으로 하여 젖소에 면역시킨 후 생산된 우유의 anti-H. pylorigkdcp의 생성능을 검토하고, 백신투여량과 항체 생성과의 관계, 항원 항체의 특이성, H. Pylori균 응집력, 항체의 산과 열에 대한 안정성, 그리고 백신투여가 젖소에 미치는 영향을 알아보았다. 1. 백신 투여량에 따른 혈청과 유청내의 anti-H. pylori항체의 함량은 10㎖, 20㎖, 30㎖ 백신투여 모든 군에서 대조구에 비해서 높은 양의 항체 생성을 확인하였다. 유의적인 차이는 없었으나 20㎖투여가 항체 생성이 가장 많은 것으로 조사되었다. 백신 투여량에 따른 유청내의 anti-H. pylori항체 생성량은 혈청에서 나타난 결과와 유사한 양상으로 형성되었다. 2. Anti-H. pylori항체의 SDS-PAGE에 의한 분자량측정 결과 heavy chain은 50kDa정도, light chain은 24kDa정도로 확인 되었다. 3. H. pylori항원 단백질의 분자량측정 결과 12개의 band가 형성되었다. Anti-H. pylori의 항원 특이성을 알아보기 위해 western bloting을 한 결과 혈청, 혈청정제, 유청, 유청 정제 모두 7개의 항원성 물질을 확인할 수 있었고, 주 항 원성 물질은 분자량이 97, 66, 34kDa 이었다. 4. 응집반응결과 유청속의 anti-H. pylori항체가 H. pylori균에 대해 1/10의 응집가를 나타내었다. 5. Anti-H. pylori항체의 산·알칼리에 대한 안정성 실험에서는 pH 5 ∼ pH 10 범위에서 안정한 상태로 100%의 활성을 나타내었다. 6. Anti-H. pylori항체의 열에 대한 안정성 실험에서는 60℃에서 60분간 안정한 상태를 보였고, 70℃에서도 비교적 안정한 상태였으나 60분 경과후 40%정도 활성이 감소하였다. 80℃에서는 4분간 처리했을 때 77%의 활성을 유지하였고, 100℃에서도 1분간은 비교적 안정한 상태였다. 7. 백신투요로 인하여 유량이 12% 감소하는 경향을 나타냈고, 최장 1주일 정도 지나면서 회복되었다. 8. 백신투여 후 젖소의 체온을 측정한 결과 대조구에 비해 정상적인 범위 내에서 체온이 상승하는 경향을 나타내었다. This study has been to examine bio-function of anti-H. pylori antibodies of milk produced from cows immune with antigen germ of Helicobacter pylori and search the relation between vaccine dosage volume and antibody formation, peculiarity of antigen antibody, cohesion of H. pylori germ, stability about add and heat of antibody, and impact of vaccine dosage on cows. The content of serum and Anti-H. pylori antibody within whey in accordance with vaccine dosage volume has confirmed the formation of high-quantified antibody compared to the controlled conditions in all groups vaccine dosages of 10㎖, 20㎖, and 30㎖. It has been turned out that the antibody was farmed most in 20㎖ dosage on while there was no attention difference. The molecular weight of Anti-H. pylori antibody measured by SDS-PAGE were turned out as about 50kDa in the heavy chain and about 24kDa in the light chain. 12 bands were formed as the result of measured molecular weight of antibody protein. The western blotting was performed in order to examine the antigen peculiarity of Anti-H. pylori that all 7 antigen substances including serum, serum refining, whey and whey refining could be confirmed and the main antigen substances were 97, 66, 34kDa of molecular weight. As a result of cohesive response Anti-H. pylori antibody in whey showed 1/10 cohesive rate about H. pylori germ. In stability test about acid and alkali of antibody there was 100% activated in the range of pH 5-pH 10. In stability test about heat it showed stable condition in 60℃: for 60 minutes and comparatively stable condition in 70℃, but reduced activation to 40% after 60 minutes. It maintained 77% activation in 80℃ for 4 minutes and comparatively stable in 100℃ for I minute. It was inclined to reduce to 12% of flow caused by vaccine injection, but recovered after about maximum 1 week. In measurement of body temperature of cows after vaccine injected, it was inclined to rise with the normal scope in comparison with the controlled conditions.

급성 골수성 백혈병 환자에서 발생한 침습성 아스페르길루스중에 대한 수술적치료 1 예

배영환;어원식;어상민;김승만 김태영;박수영;서영경;정은주;안효정 인제대학교 2011 仁濟醫學 Vol.32 No.-

Invasive pulmonary aspergillosis is known to have a poor prognosis and major cause of morbidity and motality in patients with hematologic disease. Without adequate therapy, invasive pulmonary aspergillosis will almost always progress to fatal pneumonia. Early administration of antifungal agents in patient with invasive pulmonary aspergillosis is most important. And surgical resection of aspergillous-infected tissue may be useful in patients with lesions that are contiguous with great vessel and lesions causing hemoptysis. We reported a rare case of surgical resection as curative treatment for invasive pulmonary aspergillosis patient in 58-year-old man who had the history of acute myeloid leukemia.

대학급식의 미생물적 품질보증을 위한 HACCP의 적용 : 참치샐러드 Tuna Salad

김운주,최은희,최현미,배주희,채현숙 충북대학교 교육 ·생활연구소 생활과학연구센터 2002 생활과학연구논총 Vol.6 No.1

The purpose of this study was to evaluate the microbiological quality, and to assure the safety of the food production process in the university foodservice facilities in accordance with the Hazard Analysis Critical Control Point(HACCP) concepts. The kitchen layout and time-temperature relationship, miciobiological quality(total plate count, coliform) were assessed to identify the critical point during each of the production phases. The results were as follows: 1) The kitchen layout had to be improved because contamination area was not separated from non-contamination area, and work table was in contact with washing sink. 2) Some employee did not follow personal hygiene standards(hand washing), and did not wear proper working uniforms(hair restraints). 3) The production time of tuna salad was 120 min, and environment temperature was 20.1~26.0℃. Improper receiving temperature, inproper holding practices(without cover at room temperature) were observed. 4) In the purchasing phase of the raw materials, the microbiological quality of green pepper was not at acceptable level based on the TPC(2.3×10^6CFU/g)and coliform(>14,000 MPN/g). During washing phase TPC was decreased about 3 log cycle. After cutting and holding phase the level of TPC and coliform were increased rapidly. At serving phase the microbiological quality of tuna salad were not at acceptable level(TPC 3.3×10^6 CFU/g, coliform 11,000 MPN/g) according to the standard set(TPC <10^5 CFU/g, coliform <100 MPN/g) by Sorberg et al. 5) For tuna salad, critical control points were purchasing and receiving of fresh vegetables, washing, cutting, holding, mixing and serving phase.

김치 유산균인 Lactobacillus sp. JJ-2001이 생산하는 Bacteriocin에 관한 연구

조영배,조우제,조영임,이은주,김성구,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

The microorganisms producing bacteriocin from kimch were screened, and among them,a strain showing the highest bacteriocin activity and stability was isolated. Based on the taxonomic characteristics of its morphological, cultural and physiological properties, the isolatef strain was characterized as Lactobacilius sp..To identify optimal conditions for the growth and the production of bacteriocin from the isolated strain,nutrient source, initial pH, and culture temperature were evaluated. Physiochemical properties of bacteriocin was also investigated. The potimal condition for the production of bacteriocin and the cell growth was obtained at 2% glucose, 1% beef extract as organic nitrogen source,1%(NH_4)_2SO_4 as inorganic nitrogen source,and 0.1% Tween 80. The isolated strain showed the maximum growth at 25℃. The inhibitory range of its antimicrobial substance aginst gram positive bacteria was marrow and its inhibitory activity was maintained between pH3.0 and pH8.0. The antimicrobial activity of bacteriocin from the isolated strain was stable at 50℃.

도재수복물의 표면 첨색시 온도에 따른 색조안정성과 표면조도의 평가

백은주,배태성,송광엽,박찬운 全北大學校 齒醫學硏究所 1991 전북치대논문집 Vol.9 No.1

The effects of oven firing on the color stability and surface texture of extrinsic stains used characterization and color modification of metal ceramic restoration were studied by comparing different temperatures and techniques. They were autoglaze technique, applied glaze technique, two step low-fusing glaze technique and one step low-fusing glaze technique. Autoglaze technique and applied galze technique were air-fired to manufacturer's recommended temperature. Two step low-fusing glaze technique is seperated staining and overglazing. Thin slurry of stain powder were air-fired to 1500℉. Overglaze powder was than mixed with stain medium and air-fired to 170℉. One step low-fusing glaze technique in combined staining and overglazing. Thin slurry of stain and glaze powder were air-fired to 170℉. The obtained results were as follows : 1. Slightly significant color differences by colorimeter were found between different stain application and fusion techniques(P<0.05). 2. Two step low-fusing glaze method showed the most rough surface, especially ceramco orange stain(P<0.05). 3. Surface roughness increased gradually in order of autoglaze, applied glaze, one step low-fusing glaze but they were not significantly different(P<0.05). 4. When two step low-fusing glaze was applied, both color measurement and surface texture were significantly different from other groups(P<0.05).

대전광역 시민의 골다공증 검진에 관한 고찰

유은주,이관우,박현모,이수배,김광현,강희구,박성오 한국건강관리협회 1999 건협 Report Vol.2 No.-

바터 팽대부에 생긴 유암종 1예

강명주,지삼룡,박석주,이재익,조영완,김준영,박성재,박은택,이연재,이상혁,설상영,배상균 인제대학교 2006 仁濟醫學 Vol.27 No.-

Carcinoid tumors have been reported in a wide range of organs but most commonly involve the lungs, bronchi and gastrointestinal tract. Within the gastrointestinal tract, appendix is the most common location for carcinoid, followed by the distal small intestine, the rectum, and the stomach. Among these, primary involvement of the ampulla of Vater is extremely rare. We report a case of carcinoid tumor of ampulla of Vater. A 62-year-old man presented with epigastric soreness. Gastroduodenal endoscopy showed hyperemic bulging mass on ampulla of Vater and diagnosed as carcinoid tumor by histologic finding, with immunohistochemical study. In ^(111)In-octreoscan, tumor had regional lymph node metastasis, but no evidence of distant metastasis. Pancreatoduodenectomy with lymph node dissection was performed.

한약재료(단삼, 도인, 당귀미, 솔잎) 추출물이 지방산화에 미치는 영향

김수민,김은주,조영석,배만종 경산대학교 생명자원개발연구소 1998 생명자원과 산업 Vol.2 No.-

지방산화 촉진인자인 활성산소종 (Hydrogen peroxide, Superoxide, Hydroxyl radical)과 Iron sources 들이 고혈압 치료제로 사용되고 있는 한약재료추출물들과의 반응효과를 in vitro 상에서 검토한 결과 oil emulsion 상태에서 활성산소종은 OH, H₂O₂, KO₂ 순으로 지방산화 촉진작용이 나타났으며, 한약재료추출물 자체도 산화반응을 어느정도 촉진시키는 경향이었다. 한약재료추출물의 활성산소 포집능은 KO₂에 비해 H₂O₂와 OH은 나타내지 않는 경향이었다. 그러나, 전반적으로 지방산화를 촉진시키는 ?? ion binding 능력은 추출물 모두 탁월하였다. ?? ion의 함량은 도인, 솔잎에 비해 단삼, 당귀미가 높은 수치를 나타내었으며, total iron 함량 역시 같은 경향이었다. 항산화작용을 하는 ascorbic acid 함량은 솔잎이 26.97ppm으로 가장 높은 함량을 나타내었으며, 단삼과 당귀미는 19.74, 22.14ppm으로 유사하며, 도인이 5.50ppm으로 낮은 값을 나타내었다.(P<0.05). 전자공여능은 솔잎, 단삼이 각각 79.54%,77.11%로 도인, 당귀미보다 높게 나타났고, SOD 유사활성측정에서는 도인이 0.16으로 가장 낮은 흡광도 수치를 나타내어, pyrogallol의 자동산화를 억제하는 SOD 유사활성이 다른 추출물에 비해 높은 것으로 나타났다(P<0.05). 아질산염소거작용은 pH에 따라 소거능이 다르게 나타났으며, 솔잎과 당귀미가 pH 1.2에서 각각 99.8%, 98.6%의 강한 소거능을 나타내었고, pH의 증가에 따라 전반적으로 감소하는 경향이었으며, 특히, pH6.0에서는 단삼과 도인의 경우 아질산염 소거작용이 나타나지 않았다. 이상의 결과로 한약재료 추출물 4종의 기능성은 솔잎 추출물이 가장 우수한 것으로 사료된다. This study was carried out to investigate the effects of herb extracts on lipid oxidation and free radical reaction in iron sources reacted with active oxygen species. The results were summarized as follow; The catalytic effects of active oxygen on lipid oxidation in oil emulsion tended to be showed OH, H₂O₂ and KO₂ in order. At the same time, herb extracts itself were tended to be showed a little catalytic effects. Active oxygen scavenging ability of herb extracts didn't show, but herb extracts played role as a strong chelating agents to bind iron ion if ?? ion exist in oil emulsion. The content of ?? ion Salvia miltiorrhiza Bge, and also, the content of total iron was the same persica Stokes and Pinus strobus. And also, the content of total iron was the same tendancy. The content of ascorbic acid of Pinus strobus showed the highest amounts as 26.97ppm among all herb extracts, but Salvia miltiorrhiza Bge., Angelica gigas Nakai and Prunus persica Stokes were 19.74ppm, 22.14ppm and 5.50ppm, respectively(P<0.05). Electron donating ability of Pinus strobus and Salvia miltiorrhiza Bge. were 79.54% and 77.11%, respectively, which was showed higher content than those of Prunus persica Stokes and Angelica gigas Nakai. The SOD-like activity of Prunus persica Stokes was showed optical density(O.D.) 0.16, compared to other berb extracts which means the most strong antioxidant reaction. The nitrite scavenging effects were tended to be different, depending on pH value as pH value was increased. They were decreased overall. Especially, they didn't show the nitrite scavenging effect in pH 6.0. In conculsion, the Pinus strobus extract among herb extracts were the most effective by evaluation as functional sources.

Development of a Monitoring System for Water-borne Bacteria by a Molecular Technique, PCR-RELP-sequence Analysis

이혜영,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.3

Since water borne infection causes acute diseases and results in spread of diseases by secondary infection, the prevention is very important. Therefore, it is necessary to have a method that is rapid and effective to monitor pathogenic bacteria in drinking water. In this study, we employed a systematic method, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis, to develop an effective monitoring system for possible bacterial contaminants in drinking water. For this purpose, PCR primers were derived from 992 bp region of the 16s rRNA gene that is highly conserved through the different species of prokaryotes. To test whether the PCR primers designed are indeed useful for detecting all the possible microbial contaminants in the water, the primers were used to amplify 16s rRNA regions of different microbial water-borne pathogens such as E. coli, Salmonella, Yersinia, Listeria, and Staphylococcus. As expected, all of tested microorganisms amplified expected size of PCR products indicating designed PCR primers for 16s rRNA indeed can be useful to amplify all different microbial water-borne pathogens in the water. Furthermore, to test whether these 16s rRNA based PCR primers can detect bacterial populations present in the water, water samples taken from diverse sources, such as river, tap, and sewage, were used for amplification. PCR products were for then subjected for cloning into a T-vector to generate a library containing 16s rRNA sequences from various bacteria. With cloned PCR products, RFLP analysis was done using PCR products digested with restriction enzyme such as Hae Ⅲ to obtain species-specific RFLP profiles. After PCR-RFLP, the bacterial clones which showed the same RFLP profiles were regarded as the same ones, and the clones which showed distinctive RFLP profiles were subsequently subjected for sequence analysis for species identification. By this PCR-RFLP analysis, we were able to reveal diverse populations of bacteria living in water. In brief, in unsterilized natural river water, over 60 different species of bacteria were found. On the other hand, no PCR products were detected in drinking tap-water. The results from this study clearly indicate that the PCR-RFLP-sequence analysis can be a useful method for monitoring diverse, perhaps pathogenic bacteria contaminated in water in a rapid fashion.