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Influence of Water Electrolysis on Bioelectrochemical Methane Conversion of Coal
( Dong-mei Piao ),( Young-chae Song ),( Gyung-geun Oh ),( Dong-hoon Kim ),( Yong-tae Ahn ),( Yeo-myeong Yun ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 ISSE 초록집 Vol.2019 No.-
The influence of water electrolysis on the biogenic methane conversion of coal was investigated in a bioelectrochemical anaerobic batch reactor. For the experiment, the pairs of ordinary electrodes (OE) consisting of anode and cathode were prepared with titanium foil and the surface of the OE surface was coated with a dielectric polymer to obtain a pair of insulated electrodes (IE). The pairs of OE or IE were placed in the bioelectrochemical anaerobic batch reactors, and polarized by applying voltage (0.5V, 1.0V, and 2.0V for the OEs, 2.0V for the IE) with an external voltage source. The bioelectrochemical anaerobic reactors were operated at 35oC after adding a medium containing yeast extract, coal (lignite, 2.5 g/L), and seeding with anaerobic sludge. The cumulative methane production in the OE reactor applied with the voltage of 2.0 V was gradually saturated to 162.9 mL without lag phase, which was higher 136 mL in the IE reactor. However, when the electrodes in the OE reactor were applied with a voltage (0.5V, 1.0V) lower than the voltage for water electrolysis, the cumulative methane production from coal was less than the blank. This indicates that the oxygen from the water electrolysis on the anode polarized with 2.0V was served as an electron acceptor to stimulate the ring-opening reaction of the high molecular coal hydrolysis products. However, the methane production from the OE and IE reactors was gradually decreased by the accumulation of the coal conversion intermediates. By supplementing the anaerobic medium containing yeast extract and anaerobic sludge, the cumulative methane production was abruptly increased again in the IE reactor, which was significantly higher than those of the OE reactors. The total methane yield of coal was 153.9 mL/g lignite in the IE reactor, significantly higher than 54.1 mL/g lignite in the OE reactor (2.0 V). This suggests that the coal could be effectively hydrolyzed and fermented under the electrostatic field in the IE reactor, and the methane conversion of coal via biological direct interspecies electron transfer could be stimulated by supplementing yeast extract and anaerobic sludge.
Piao, Mei Jing,Yoon, Weon Jong,Kang, Hee Kyoung,Yoo, Eun Sook,Koh, Young Sang,Kim, Dong Sam,Lee, Nam Ho,Hyun, Jin Won Molecular Diversity Preservation International (MD 2011 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.12 No.11
<P>The aim of this study was to investigate the cytoprotective properties of the ethyl acetate fraction of <I>Sargassum muticum</I> (SME) against ultraviolet B (UVB)-induced cell damage in human keratinocytes (HaCaT cells). SME exhibited scavenging activity toward the 1,1-diphenyl-2-picrylhydrazyl radicals and hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) and UVB-induced intracellular reactive oxygen species (ROS). SME also scavenged the hydroxyl radicals generated by the Fenton reaction (FeSO<SUB>4</SUB> + H<SUB>2</SUB>O<SUB>2</SUB>), which was detected using electron spin resonance spectrometry. In addition, SME decreased the level of lipid peroxidation that was increased by UVB radiation, and restored the level of protein expression and the activities of antioxidant enzymes that were decreased by UVB radiation. Furthermore, SME reduced UVB-induced apoptosis as shown by decreased DNA fragmentation and numbers of apoptotic bodies. These results suggest that SME protects human keratinocytes against UVB-induced oxidative stress by enhancing antioxidant activity in cells, thereby inhibiting apoptosis.</P>
Antioxidative Constituents from Buddleia officinalis
Piao, Mei-Shan,Kim, Mi-Ran,Lee, Dong-Gun,Park, Yoonkyung,Hahm, Kyung-Soo,Moon, Young-Hee,Woo, Eun-Rhan The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.6
Four flavonoids (1-4), a phenylethyl glycoside (5), and a phenylpropanoid glycoside (6) were isolated from the flowers of Buddleia officinalis (Loganiaceae). Their structures were determined by chemical and spectral analysis. Among the isolated compounds, luteolin (1) and acteoside (6) exhibited the most potent antioxidative activity on the NBT superoxide scavenging assay. In addition, compounds 1-6 revealed weak antifungal activity, and no hemolytic activity.
Piao, Mei Jing,Kang, Hee-Kyoung,Yoo, Eun-Sook,Koh, Young-Sang,Kim, Dong-Sam,Lee, Nam-Ho,Hyun, Jin-Won The Korean Society for Applied Biological Chemisty 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.2
Photo-protective properties of Polysiphonia morrowii ethanol extract (PME) against ultraviolet B (UVB) radiation-induced cellular damage were investigated in human HaCaT keratinocytes. PME exhibited scavenging activity against 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species induced by either hydrogen peroxide ($H_2O_2$) or UVB radiation. In addition, PME scavenged superoxide anion generated by xanthine/xanthine oxidase system and hydroxyl radical generated by the Fenton reaction ($FeSO_4+H_2O_2$), both of which were detected using electron spin resonance spectrometry. Moreover, PME, which scavenges $H_2O_2$, was shown to contain the antioxidant compound 3-bromo-4,5-dihydroxylbenzaldehyde, possibly accounting for scavenging actions. PME also restored the levels of catalase and superoxide dismutase protein expression and activity that were decreased by UVB radiation. PME reduced UVB-induced apoptosis, as shown by decreased apoptotic bodies and DNA fragmentation. These results indicate that PME protects human keratinocytes against oxidative stress induced by UVB radiation through the enhancement of antioxidant systems.
김용길,Piao, Dong-Mei 원광대학교 법학연구소 2008 圓光法學 Vol.24 No.3
Recently the Chinese government has carried out appropriate coordination between the planned economy and the market economy, in order to narrow the distinction in wealth between the wealthy and the poor. The Chinese property law has been enacted by arranging the existing security law, the real property law, general provisions of the civil code and so on. In Chinese property law, the system of good faith acquirement of real property established based on public presumption credibility and demonstrative effect of registry in order to consummate China's transaction safety protection system. The system for real estate registration in china was in charge of an each local executive agency, but not the court. This system was involved with many problems for a nationwide unified enforcement. The Law of Reality of China enacted anew is only to provide for basic principle for the real estate registration. The detailed process and proceedings in the concrete for the unified enforcement of real estate registration is to improve over the short haul. After solving the matters in dispute these days, China has been more and more grown rapidly by establishing the Chinese property law and the good faith acquirement of the real property now.
Stimulation of keratinocyte differentiation by extract of combined medicinal plant
Xue Mei Li,Dae-Kyoung Choi,Seul Ki Lim,In Suk Ahn,Dong-Il Kim,Yong Jun Piao,Chang Deok Kim,Jeung-Hoon Lee 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.4
Skin is the outermost organ and acts as a barrier between the organism and environment. Skin protects the organism from environmental insults, such as chemicals, pathogens, and UV light. Much of the protective function of skin is dependent on the epidermis, a multi-layered epithelium that is composed of various cell types such as keratinocytes and melanocytes. Keratinocytes produce protective components through a sophisticated differentiation process. Disturbance of keratinocyte differentiation is related to several skin diseases such as psoriasis and atopic dermatitis. In this study, we prepared extract of combined medicinal plants (ECMP) consisting of Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia, Glycyrrhiza uralensis Fischer, and root bark of Ulmus davidiana. We demonstrated that ECMP enhanced keratinocyte differentiation and barrier functionality using an in vitro cell culture system and in vivo animal test. Treatment of cultured keratinocytes with ECMP resulted in induction of keratinocyte differentiation, as evidenced by increased differentiation markers such as involucrin, loricrin, and filaggrin. In line with these results, ECMP decreased proliferation of keratinocytes cultured in vitro. ECMP applied topically to tape-stripped mouse skins accelerated reduction of transepidermal water loss (TEWL), indicating fast recovery of barrier function. Immunohistochemistry showed that ECMP increased the filaggrin level in tape-stripped mouse skins. These results suggest that ECMP may be applicable for keratinocyte differentiation-related skin diseases.