http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Whole-genome resequencing analysis of 20 Micro-pigs
Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3
Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.
정다현,김예희,김지은,이예영,강현주 순천향대학교 기초과학연구소 2021 순천향자연과학연구 논문집 Vol.27 No.1.2
The purpose of this study was to analyze risk of falls between elderly people and college students. The subjects of this study were 25 people aged 70 years or older and 17 college students located in Asan. We measured sensory, integrated balance, nervous, musculoskeletal system and HQR by FRA system (Inbody, Korea). The results of measurement showed significant differences in sensory system (p<.01), integrated balance (p<.001), nervous system (p<.001), and musculoskeletal system (p<.001) between groups. These results suggest that aging makes difference of function of these systems. In our view, counterplans need to be implemented, including exercise intervention to prevent decreases of fall risk,
이예영,김지은,서예은,정다연,강현주 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2
Muscle fatigue is a symptom that decreases your muscles’ ability to perform over time. It can be associated with a state of exhaustion, often following excessive sports activity or exercise. While exercise is a common cause of muscle fatigue, this symptom can be the result of other health conditions. Muscle fatigue caused by excessive activity limits activities of daily living and difficult to maintain a stable posture. Therefore, the purpose of this study was to investigate the effect of stretching, icing, and sports massage on muscle fatigue recovery.
Recurrence of pulmonary thromboembolism during anticoagulation in lung cancer patient
( Da Hyun Kang ),( Se Yeon Park ),( Sung Soo Jung ),( Ju Ock Kim ),( Chae Uk Chung ) 대한내과학회 2015 대한내과학회 추계학술대회 Vol.2015 No.1
Introduction: Patients with cancer have higher risk of thromboembolism as compared with normal subjects. Most guidelines recommend low-molecular-weight heparins or vitamin K antagonists, but they have several limitations. New oral anticoagulants (NOACs) are another treatment option in prevent thromboembolism, but effectiveness for patients with cancer is not clear. We describe a patient with lung cancer and pulmonary thromboembolism treated with NOACs that was exacerbated by switch to vitamin K antagonists. Case: A 63-year-old female was diagnosed with lung adenocarcinoma as T4N3M1a stage. She was initially presented with multifocal endoluminal filling defects in interlobar and segmental arteries in BLL, diagnosed as pulmonary thromboembolism (Fig. 1A). She was treated with rivaroxaban, direct factor Xa inhibitor for thromboembolism, and gefitinib for lung cancer. Chest CT after 3 months showed that the thromboembolism was markedly improved (Fig. 1B). Because of cost, we substituted warfarin for rivaroxaban after 6 months. Unfortunately, switch towarfarin resulted in recurrence of pulmonary thromboembolism in 3 months despite of high level of INR (Fig. 1C). She received the combination therapy of unfractionated heparin with warfarin in acute phase, and prescribed rivaroxaban again, 5 days later. After 2 months, thromboembolism was resolved (Fig. 1D). Conclusions: This case report suggests that rivaroxaban, one of NOACs, may be feasible anti-coagulation therapy for pulmonary thromboembolism with lung cancer.
GDF-15 Levels Predict Efficacy in Lung Cancer Patients Treated with PD-1/ PD-L1 Inhibitors
( Da Hyun Kang ),( Pureum Sun ),( Chaeuk Chung ),( Song-i Lee ),( Dongil Park ),( Jeong Suk Koh ),( Yoonjoo Kim ),( Jeong Eun Lee ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.0
Background Growth and differentiation factor 15 (GDF-15) is a divergent member of the TGF-β superfamily. In disease states, such as acute injury, inflammation, and cancer, GDF-15 expression is dramatically increased. In cancer patients, elevated GDF-15 levels correlate with poor prognosis. In this study, we investigated whether GDF-15 levels can predict the treatment response of patients with advanced non-small cell lung cancer (NSCLC) treated with PD-1/PD-L1 inhibitors. Methods This study included patients who were diagnosed with NSCLC and treated with PD-1/PD-L1 inhibitors at Chungnam National University Hospital from March 2018 to May 2020. We evaluated plasma GDF-15 levels and correlated the findings with clinical outcomes. Results In our cohort of 89 NSCLC patients, the mean plasma GDF-15 levels were 2475.86 pg/mL (794.07-4901.25). The objective response rate (ORR) was significantly higher in those with low GDF-15 (<1520 pg/mL) than those with high GDF-15 (ORR 35.5% vs. 15.5%, p=0.032). In 47 patients with blood sample post 2 months after treatment, the ORR and disease control rate (DCR) were significantly higher in patients with decreased GDF-15 levels compared to those with increased follow up GDF-15 levels (ORR 26.7% vs. 5.9%, p=0.028; DCR 73.3% vs. 35.3%, p=0.011). Conclusion Plasma GDF-15 levels could be a potential biomarker for predicting the efficacy in NSCLC patients treated with PD-1/PD-L1 inhibitors.
The effect of the DLTIDDSYWYRI motif of the human laminin α2 chain on implant osseointegration
Kang, Hyun Ki,Kim, O. Bok,Min, Seung-Ki,Jung, Sung Youn,Jang, Da Hyun,Kwon, Taek-Ka,Min, Byung-Moo,Yeo, In-Sung Elsevier 2013 Biomaterials Vol.34 No.16
<P><B>Abstract</B></P> <P>Considerable effort has been directed towards replacing lost teeth using tissue-engineering methods such as titanium implants. A number of studies have tried to modify bioinert titanium surfaces by coating them with functionally bioactive molecules for faster and stronger osseointegration than pure titanium surfaces. Recently, peptides have been recognized as valuable scientific tools in the field of tissue-engineering. The DLTIDDSYWYRI motif of the human laminin-2 α2 chain has been previously reported to promote the attachment of various cell types; however, the <I>in vivo</I> effects of the DLTIDDSYWYRI motif on new bone formation have not yet been studied. To examine whether a laminin-2-derived peptide can promote osseointegration by accelerating new bone formation <I>in vivo</I>, we applied titanium implants coated with the DLTIDDSYWYRI motif in a rabbit tibia model. The application of the DLTIDDSYWYRI motif-treated implant to tibia wounds enhanced collagen deposition and alkaline phosphatase expression. It significantly promoted implant osseointegration compared with treatment with scrambled peptide-treated implants by increasing the bone-to-implant contact ratio and bone area. These findings support the hypothesis that the DLTIDDSYWYRI motif acts as an effective osseointegration accelerator by enhancing new bone formation.</P>
( Da Hyun Jung ),( Jie-hyun Kim ),( Su Jin Jeong ),( Soon Young Park ),( Il-mo Kang ),( Kyoung Hwa Lee ),( Young Goo Song ) 대한소화기학회 2018 Gut and Liver Vol.12 No.6
Background/Aims: Helicobacter pylori eradication rates are decreasing because of increases in clarithromycin resistance. Thus, finding an easy and accurate method of detecting clarithromycin resistance is important. Methods: We evaluated 70 H. pylori isolates from Korean patients. Duallabeled peptide nucleic acid (PNA) probes were designed to detect resistance associated with point mutations in 23S ribosomal ribonucleic acid gene domain V (A2142G, A2143G, and T2182C). Data were analyzed by probe-based fluorescence melting curve analysis based on probe-target dissociation temperatures and compared with Sanger sequencing. Results: Among 70 H. pylori isolates, 0, 16, and 58 isolates contained A2142G, A2143G, and T2182C mutations, respectively. PNA probe-based analysis exhibited 100.0% positive predictive values for A2142G and A2143G and a 98.3% positive predictive value for T2182C. PNA probe-based analysis results correlated with 98.6% of Sanger sequencing results (κ-value=0.990; standard error, 0.010). Conclusions: H. pylori clarithromycin resistance can be easily and accurately assessed by dual-labeled PNA probe-based melting curve analysis if probes are used based on the appropriate resistance-related mutations. This method is fast, simple, accurate, and adaptable for clinical samples. It may help clinicians choose a precise eradication regimen. (Gut Liver 2018;12:641-647)
Kang, Hyun Ki,Min, Seung-Ki,Jung, Sung Youn,Jung, Kyoungsuk,Jang, Da Hyun,Kim, O Bok,Chun, Gae-Sig,Lee, Zang Hee,Min, Byung-Moo D.A. Spandidos 2011 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.28 No.6
<P>Although previous studies indicate that skin-derived precursors (SKPs) are multipotent dermal precursors that share similarities with neural crest stem cells (NCSCs), a shared ability for multilineage differentiation toward neural crest lineages between SKPs and NCSCs has not been fully demonstrated. Here, we report the derivation of SKPs from adult mouse skin and their directed multilineage differentiation toward neural crest lineages. Under controlled in vitro conditions, mouse SKPs were propagated and directed toward peripheral nervous system lineages such as peripheral neurons and Schwann cells, and mesenchymal lineages, such as osteogenic, chondrogenic, adipogenic, and smooth muscle cells. To ask if SKPs could generate these same lineages in vivo, a mixture of SKP-derived mesenchymal stem cells and hydroxyapatite/tricalcium phosphate was transplanted into the rat calvarial defects. Over the ensuing 4 weeks, we observed formation of osteogenic structure in the calvarial defect without any evidence of teratomas. These findings demonstrate the multipotency of adult mouse SKPs to differentiate into neural crest lineages. In addition, SKP-derived mesenchymal stem cells represent an accessible, potentially autologous source of precursor cells for tissue-engineered bone repair.</P>