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Promoted Growth of Maize by the Phosphate Solubilizing Bacteria Isolated from North-east China
Wu, Hai-Yan,Wang, Li-Chun,Gao, Xing-Ai,Jin, Rong-De,Fan, Zuo-Wei,Kim, Kil-Yong,Zhao, Lan-Po Korean Society of Soil Science and Fertilizer 2011 한국토양비료학회지 Vol.44 No.1
A strain of phosphate solubilizing bacterium was isolated from rhizosphere and identified as Burkholderia sp. by 16S-rRNA gene sequence analyses. The bacterium was found to release gluconic acid and the solubilization of hydroxyapatite in the liquid medium by a significant drop in pH to 3.7 from an initial pH 7.0. The soluble-P concentration continuously increased during the incubation periods and the total amount of soluble P released in culture filtrate was detected at 990 mg $L^{-1}$ after 10 days of inoculation. Most promoted maize growth was found in the standard NPK (240-120-120 kg $ha^{-1}$) soil inoculation with Burkholderia sp. (Twenty milliliters/plant, 106 CFU) and also in the absence of Burkholderia sp. inoculation, the soil amended with only 2/3 levels of P gave significant higher plant yield compared to 1/3 levels of P or without P supplementation.
Wu, Dong-Ming,Zhang, Peng,Xu, Guang-Chao,Tong, Ai-Ping,Zhou, Cong,Lang, Jin-Yi,Wang, Chun-Ting Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4
Pemetrexed is an antifolate agent which has been used for treating malignant pleural mesothelioma and non small lung cancer in the clinic as a chemotherapeutic agent. In this study, pemetrexed inhibited cell growth and induced G1 phase arrest in the A549 cell line. To explore the molecular mechanisms of pemetrexed involved in cell growth, we used a two-dimensional polyacrylamide gel electrophoresis (2-DE) proteomics approach to analyze proteins changed in A549 cells treated with pemetrexed. As a result, twenty differentially expressed proteins were identified by ESI-Q-TOF MS/MS analysis in A549 cells incubated with pemetrexed compared with non-treated A549 cells. Three key proteins (GAPDH, HSPB1 and EIF4E) changed in pemetrexed treated A549 cells were validated by Western blotting. Accumulation of GAPDH and decrease of HSPB1 and EIF4E which induce apoptosis through inhibiting phosphorylation of Akt were noted. Expression of p-Akt in A549 cells treated with pemetrexed was reduced. Thus, pemetrexed induced apoptosis in A549 cells through inhibiting the Akt pathway.
Promoted Growth of Maize by the Phosphate Solubilizing Bacteria Isolated from North-east China
Hai-Yan Wu,Li-Chun Wang,Xing-Ai Gao,Rong-De Jin,Zuo-Wei Fan,Kil-Yong Kim,Lan-Po Zhao 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.1
A strain of phosphate solubilizing bacterium was isolated from rhizosphere and identified as Burkholderia sp. by 16S-rRNA gene sequence analyses. The bacterium was found to release gluconic acid and the solubilization of hydroxyapatite in the liquid medium by a significant drop in pH to 3.7 from an initial pH 7.0. The soluble-P concentration continuously increased during the incubation periods and the total amount of soluble P released in culture filtrate was detected at 990 mg L<SUP>-1</SUP> after 10 days of inoculation. Most promoted maize growth was found in the standard NPK (240-120-120 kg ha<SUP>-1</SUP>) soil inoculation with Burkholderia sp. (Twenty milliliters/ plant, 106 CFU) and also in the absence of Burkholderia sp. inoculation, the soil amended with only 2/3 levels of P gave significant higher plant yield compared to 1/3 levels of P or without P supplementation.
Human fetal anatomy of the coccygeal attachments of the levator ani muscle
Niikura, Hitoshi,Jin, Zhe Wu,Hwan Cho, Baik,Murakami, Gen,Yaegashi, Nobuo,Lee, Jong Kyun,Lee, Nae Ho,Li, Chun Ai Wiley Subscription Services, Inc., A Wiley Company 2010 Clinical Anatomy Vol.23 No.5
<P>In contrast to the attachments to the pubis and rectum, there is little information on fetal development of the coccygeal attachment of the levator ani muscles. We find that at 9 weeks, the coccygeus muscle is a large muscle facing the piriformis or gluteus maximus and inserting onto the ischial spine, whereas the levator ani is restricted to the area near the pubis. By 12 weeks, the levator ani also obtains attachment to the ischial spine immediately ventral to the coccygeus muscle. The most superior part of the coccygeus muscle occupies a space at an angle between the pelvic splanchnic and pudendal nerves. Notably, medial to the coccygeus muscle, a third parasagittal muscle (previously termed the sacrococcygeus anterior) appears by 12 weeks, increases in mass by 18 weeks, and connects and mixes with the dorsal end of the levator ani by 18–20 weeks. Thus, the coccygeal attachment of the levator ani appears not to depend on the dorsal extension of the muscle itself but on fusion with the sacrococcygeus anterior. Therefore, the final levator sheet is formed medial (internal) to the coccygeus muscle and originates from two distinct anlage. Clin. Anat. 23:566–574, 2010. © 2010 Wiley-Liss, Inc.</P>