비소세포 폐암종에서 Cyclin D1, p21 및 p53단백의 발현과 예후와의 관계

양석우,조상호,양우익,정우희,안철민,이두연 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.12

유연한 수리계획 작성을 위한 모형관리자의 설계 및 구현

양영철,장양자,김기동,박진우 한국경영과학회 2001 한국경영과학회 학술대회논문집 Vol.- No.1(2)

Recently, in the competitive environments, every company recognizes the importance of planning models. However, there are so many problems in correctly applying mathematical model to the real world. Because mathematical modeling packages, especially, charge planning managers with understanding the models and responsibility for generating plans, fast and accurate model cannot be generated with ease. In this paper, we design the system that helps planning managers flexibly create and modify mathematical models and manage model versions. Also, we implement this model management system with model base concept.

급성충소돌기염의 임상 : 병리학적 고찰

양기화,조준필,하영천,한진우,정학재,손수창,이성희,황재훈,신철호 최신의학사 1989 最新醫學 Vol.32 No.4

Mycobacterium fortuitum-chelonei complex 균체항원의 민감성과 특이성

이현우,최철순,정상인,양용태 중앙대학교 의과대학 의과학연구소 1985 中央醫大誌 Vol.10 No.1

Antigenic preparations were made from seven strains of Mycobacterium fortuitum-chelonei complex(MFCC) extracted with phosphate buffered saline(PBS extracts), with 10%, trichloroacetic acid solution (TCA extracts) and with veronal buffered saline solution containing 0.1% Triton X-100(Triton extracts). Serologic reactivities of the antigenic preparations were assayed for immune precipitation by Ouchterlony double diffusion in-gel technique against antisera of rabbits immunized with M. fortuitum TMC 1529 and M. chelonei TMC 1544. Antigenic relationships between Triton extracts of M. fortuitum and M. chelonei were analyzed by two-dimensional immunoelectrophoresis(2D-IE) and fused rocket immunoelectrophoresis(FRIE). The results obtained are summarized as follows: 1. Of antigenic preparations of PBS extracts, TCA extracts and Triton extracts, the Triton extracts and the PBS extracts proved to be active for immune precipitation, but the TCA extracts was inactive. The precipitation reaction of Triton extracts was more sensitive than PBS extracts, and the precipitation reaction of Triton extracts was enhanced by disintegration of cells with sonification, but the cross reaction was also increased. 2.' The Triton extracts of 5 strains of M. fortuitum exhibited 9 common precipitinogens with reference serum against TMC 1529 and one common precipitinogen with reference serum against TMC 1544. but the antigens of TMC 1544 with or without disintegration of cells gave a specific precipitinogen with reference serum against TMC 1544 in addition to a common precipitinogen. 3. Although the electrophoretic patterns of Triton extracts obtained from different strains and species were not demonstrated, 7 strains of the MFCC could be divided into four groups by the relative electrophoretic mobilities(REM) of the antigens with respect to bovine serum albumin(REM value); group Ⅰ(REM =0.18) 2 strains, group Ⅱ(REM = 0.27) 2 strains, group Ⅲ (REM = 0.38) 2 strains and group Ⅳ(REM = 0.49) 1 strain, but the existence of any significant relationship between the REM value and species was not recognized. 4. By means of FRIE, the presence of 3 to 5 common precipitinogens and 1 to 2 species-specific precipitinogens were observed in the Triton extracts of M. fortuitum and M. chelonei. 5. By the use of the reference antisera with a titre of at least 1 : 80 against M. fortuitum TMC 1529 and M. chelonei TMC 1544, the strains of M. fortuitum and M. chelonei could be differentiated by FRIE. In conclusion, this study established that the Triton extracts of the MFCC proved to be active for immune precipitation and contained species-specific procipitinogens, and reproducible amounts of such an antigen for immunodiffusion test could be easily prepared with one plate of heavy cultures.

Pseudomonas sp.β-1,4-Glucosidase유전자의 발현

김양우,정영철,강인수,노종수 진주여자전문대학 1995 論文集 Vol.18 No.-

The Pseudomonas β-glucosidase gene was subcloned in a IPTG-induced E. coli expression vector PKK 223-3 containing the tac promoter. Recombinant plasmid inserted β-glucosidase gene in EcoRI site of pKK 223-3 was constructed and named pKKB 10. The E. coli JM109 carrying pKKB10 was grown under antibiotic selection to mid-log phase. Each culture was divided and IPTG was added to one portion. The concertration of inducer necessary for maxium induction of β-glucosidase activity was 6mM isopropyl-β-D-thiogalactoside(IPTG) and the addition of IPTG had no inhibitiory effect on cell growth. A 8-fold increase in the level of β-glucosidase activity expressed by pKKB10 was detected 5 hours after 6mM IPTG induction. The Pseudomonas β-glucosidase gene was also subcloned in a temperature-regulated E. coli expression vector PAS1 containing the leftward promoter pL of bacteriophage lambda. Recombinant plasmid inserted β-glucosidase gene in BamHI linker of PAS1 was constructed and named PASG20. The level of gene expression in E. coli N 4830-1 habouring PASG20 was controlled by thermal inactivation of the heat-sensitive lambda c1857 repressor. Under optimal condition, the recombinant β-glucosidase gene was expressed to a level of 6.2-fold.

세가지 형상의 부유식 방파제 특성에 대한 비교연구

조원철,한덕만,양우석,이진원 중앙대학교 기술과학연구소 2000 기술과학연구소 논문집 Vol.30 No.-

본 연구는 2차원 유한요소모델을 이용한 부유식 방파제의 파랑감쇠특성에 대한 이론적 연구이다. 유체의 운동은 선형적이고, 2차원적인 포텐셜 흐름으로 가정하고 수치해석을 수행하였으며, 2차원 유한요소해석과 무한요소해석을 채택하여 부유식 방파제 영역에 적용하였다. 따라서 2개의 영역에 있어 방파제 근처 영역에서는 유한요소해석을 적용하였으며, 그 외의 영역에서는 무한요소해석을 사용하였다. 방파제의 형식은 직사각형, 원형, 사다리꼴의 세가지 형상으로 하였으며, 그 결과를 기존의 실험결과와 비교하였다. A theoretical investigation on the wave-attenuation characteristics of floating breakwaters are presented. The fluid motion is idealized as linearized, two dimensional potential flow. A two-dimensional finite element model is adopted to study the performance of floating breakwaters. The near field region surrounding the solid body is modeld using conventional finite elements, however, the far field region is represented appling the infinite elements. Numerical analysis is carried out for floating breakwaters with three different cross-sectional shapes, rectangular, circular and trapezoidal shapes, respectively. Comparison with hydraulic experimental results measured in the past is carried out to prove the validity of the numerical results.

분절호선법으로 상하악 절치부 압하 시 순측경사도가 미치는 영향에 관한 3차원 유한요소법적 연구

김동우,양훈철,김기태,김성식,손우성 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.4

본 연구는 상하악 절치부의 압하를 도모하기 위한 장치의 하나인 분절호선장치를 사용하여 절치부의 순측경사에 따른 저항중심의 위치와 변화양상, 그리고 치축경사 개선과 압하를 동시에 이루기 위해 필요한 최소 후방 견인력의 크기 및 변화양상을 알아보기 위하여 3차원 유한요소법을 이용하여 시행되었으며 다음과 같은 결론을 얻었다. 1. 치축 경사도의 변화에 따른 상악 절치부 저항중심은 1) 정상 치축경사를 가진 경우에서 측절치 브라켓 원심면 후방 6mm에 위치하였다. 2) 순측경사가 10˚ 증가된 경우에서 측절치 브라켓의 원심면 후방 9mm에 위치하였다. 3) 순측경사가 20˚ 증가된 경우에서 측절치 브라켓 원심면 후방 12mm에 위치하였다. 4) 순측경사가 30˚ 증가된 경우에서 측절치 브라켓 원심면 후방 16mm에 위치하였다. 2. 치축 경사도의 변화에 따른 하악 절치부 저항중심은 1) 정상 치축경사를 가진 경우에서 측절치 브라켓 원심면 후방 10mm에 위치하였다. 2) 순측경사가 10˚ 증가된 경우에서 측절치 브라켓 원심면 후방 13mm에 위치하였다. 3) 순측경사가 20˚ 증가된 경우에서 측절치 브라켓 원심면 후방 15mm에 위치하였다. 4) 순측경사가 30˚ 증가된 경우에서 측절치 브라켓 원심면 후방 18mm에 위치하였다. 3. 응력 분포 양상은 1) 각 저항중심에서 압하력을 가한 경우에 치주인대에 균일한 압축응력을 나타내었다. 2) 후방 견인력을 동시에 적용한 경우에 순측경사가 증가할수록 응력분포 양상이 복잡해지는 양상을 보였다. 4. 상하악 절치부가 20˚까지 순측경사된 경우에서 pure intrusion을 위하여 필요한 후방 견인력의 크기도 증가하였다. This study was designed to investigate the position of anteroposterior center of resistance for genuine intrusion and the mode of change of the minimum distal force for simultanous intrusion and retraction of the upper and lower incisors according to the increase of labial inclination. For this purpose, we used the three-piece intrusion arch appliance and three-dimensional finite element models of upper and lower incisors. 1. Positions of the center of resistance in upper incisors according to the increase of the labial inclination were as follows; 1) In normal inclination situation, the center of resistance was located in 6mm behind the distal surface of the lateral incisor bracket. 2) In 10˚ increase of the labial inclination situation, the center of resistance was located in 9mm behind the distal surface of the lateral incisor bracket. 3) In 20˚ increase of the labial inclination situation, the center of resistance was located in 12m behind the distal surface of the lateral incisor bracket. 4) In 30˚ increase of the labial inclination situation, the center of resistance was located in 16m behind the distal surface of the lateral incisor bracket. 2. Positions of the center of resistance in lower incisors according to the increase of the labial inclination were as follows; 1) In normal inclination situation, the center of resistance was located in 10mm behind the distal surface of the lateral incisor bracket. 2) In 10˚ increase of the labial inclination situation, the center of resistance was located in 13mm behind the distal surface of the lateral incisor bracket. 3) In 20˚ increase of the labial inclination situation, the center of resistance was located in 15m behind the distal surface of the lateral incisor bracket. 4) In 30˚ increase of the labial inclination situation, the center of resistance was located in 18m behind the distal surface of the lateral incisor bracket. 3. The patterns of stress distribution were as follows; 1) There were even compressive stresses in and periodontal ligament when intrusion force was applied through determined center of resistance. 2) There were gradual increase of complexity in compressive stress distribution pattern with increase of the labial inclination when intrusion and retraction force were applied simultaneously. 4. With increase of the labial inclination of the upper and lower incisors, the position of the center of resistance moved posteriorly. And the distal force for pure intrusion was increased until 20˚ increase of the labial inclination.

남자대학생들의 피부관리실태와 관련요인 조사연구

배향선,남철현,김성우,최연희,강영숙,김선혜,구재희 慶山大學校 保健福祉硏究所 2003 保健福祉硏究 Vol.8 No.-

This study was conducted to examine aspects of skin care of male students at colleges and universities of male students at colleges and universities and its related factors in order to provide informations on skin care for men. The subjects of this study was 600 men who were students at colleges and universities in Daegu City and Kyungbuk Province. Data were collected from May 1, 2002 to June 30, 2002. The results of this study can be summarized as follows. 1. According to the respondents' socio-demographic characteristics, 55.9% of them was 20 to 24 years old and 29.6% was more than 25 years old. 84.9% of them was university students, while 15.1% was college students. 25.3% belonged to the lower class, while 22.4% belonged to the upper class. 52.7% did not have a girl friend, while 47.3% has a girl friend. 41.8% lived in big cities and 48.1% lived in small and medium cities. 2. 63.0% of the respondents replied that they were in good health, while 8.8% of them were in bad health. 41.9% of them was concerned about face and body skin care, while 14.1% was not concerned about it. Among most serious skin problems, the proportion of acne was highest (44.5%). 3. Among the persons who advised the respondents when they selected cosmetics, the proportion of selecting cosmetics as their own will was highest (44.7%). 25.3% of them selected it by recommendation of female family members and 18.3% selected it by recommendation of the girl friend. In the case of selecting it as their own will, the older they were, the higher the proportion was. In the case of having the girl friend, the proportion of selecting cosmetics by recommendation of the girl friend was higher than that of selecting it by recommendation of female family members. 4. 90.4% of the respondents replied that they never visited skin care rooms, while only 9.6% of them replied that they ever visited them. In the case of visiting skin care rooms, the proportion of the upper class was higher than that of the lower class. 5. According to the frequency of visiting skin care rooms, the proportion of once a week was highest (51.0%). 29.4% of them visited them once per 6 months and 19.6% visited them once a month. 6. In the case of the reasons they did not visit the skin care rooms, the proportion of 'no interest' was highest (36.6%). 23.2% did not visit them because they thought the fee for skin care was expensive and 19.5% did not visit them because they thought the skin care room were only for women. 7. In the variables of giving advice concerning skin care, the proportion of 'their own' was highest (29.8%). The proportion of female family members was 28.2% and the proportion of the girl friend was 27.7%. In the case of the age related to having the girl friend's advice, the proportion of 'over 25 years old' was 31.9% and the proportion of '20 years old to 24 years old' was 26.4%. The proportion of 'less than 19 years old' was 23.5%. The older they were, the more they had the girl friend's advice. 8. According to the sources of getting information on skin care, the proportion of mass media (TV, Radio etc.) was highest (36.7%). 26.0% of them obtained them through the girl friend and 13.9% obtained them through female family members. Only 8.4% got them through magazines. 9. The factors influencing the experience of visiting skin care rooms were experience of side effect of foundation cosmetics, concern about skin care, expiration date of cosmetics or checking of directions of using cosmetics, donsideration of skin character when using soap, and regular exercise for health. From above results, it can be concluded that skin care and beauty specialists working in beauty academies or the department of cosmotology at colleges or universities must make efforts to change the negative recognition of male skin care through deep and comprehensive research. It is also necessary to develop systematic education course and institutionalize the qualification management system.

단백질 분해효소 생성 호염성세균의 분리 및 효소 생성

정영철,김양우,노종수,강인수 진주여자전문대학 1996 論文集 Vol.19 No.-

For the accelerating fermentation of high salt anchovy using halophilic proteolytic microorganisms, various halotolerant or halophilic protease-producing strains were isolated from 13 types of high salt fermented fish pastes. Among 13 halotolerant and 4 halophilic isolates which produced proteases on PS agar medium containing a different NaCl concentration from 0% to 20%, one halotolerant and protease-producing strain, CK-14 was identified as a member of Bacillus sp., and another halophilic and protease-producing strain , LF-7, a member of Vibrio sp. through fatty acid composition analysis. The NaCl range for growth and protease production of Bacillus sp. CK-14 and Vibrio sp. LF-7 in PS broth was extended from 0% to 12.5%, and from 5% to 20%, with an optimal concentration of 5% and 7.5%, respectively. The optimal temperature and pH for cell growth and protease production of Bacillus sp. CK-14 and Vibrio sp. LF-7 were 30℃ and 35℃, pH range of 8.0 to 8.5 and 6.5 to 9.5, respectively. The maximal protease activity of Bacillus sp. CK-14 was 3200 unit after 6% incubation, and that of Vibrio sp. LF-7 was 3980 unit after 120hr incubation under optimal culture conditions.

구치의 경사도와 회전정도가 발치공간 폐쇄시 치근막의 응력분포와 치아의 초기이동에 미치는 영향에 대한 3차원 유한요소법적 연구 : Three dimensional finite elements study

권대우,손우성,양훈철 대한치과교정학회 2004 대한치과교정학회지 Vol.34 No.5

본 연구는 상악 제1대구치에 발치공간 폐쇄를 위한 근심력 적용시, 대구치의 근원심 경사도와 회전정도의 변화가 치근막의 응력분포와 치근막 내에서의 초기 치아이동에 어떤 변화를 야기하는지를 살펴보기 위하여 시행되었다. 상악 제1대구치와 치근막의 3차원 유한요소모델을 제작하여 20˚의 근심경사와 원심경사를 설정하고. 20˚의 근심회전과 원심회전을 설정하였다. 각각의 상황에서 치체이동을 유도하는 근심력을 가하기 위해 협면에 rigid element를 부착하고 200g의 근심력과 9:1의 countertipping 모멘트. 5:1의 counterrotation 모멘트를 부여하였다. 각각의 응력분포 및 초기이동 양상을 비교하여 다음과 같은 결론을 얻었다. 상악 제1대구치의 경사도 변화나 회전 변화를 통해 근심력 적용시 구치의 근심이동에 대한 저항의 증가를 얻을 수 없었다. 20˚의 근심경사와 20˚의 원심회전이 매우 유사한 응력분포 및 변위를 나타내었다. 20˚의 원심경사와 20˚의 근심회전도 마찬가지로 유사성을 보였다. 20˚ 근심경사 및 20˚ 원심회전된 경우 치근이 협측 아동하고 치관이 설측 이동하는 관상면 상에서의 회전경향이 발생하였다. 20˚ 원심경사 및 20˚ 근심회전된 경우 치근이 설측 이동하고, 치관이 협측 이동하는 관상면 상에서의 회전경향이 발생하였다. 경사되거나 회전된 치아에 근심력을 가해야 할 경우, 관상면상에서의 회전을 방지하기 위한 부가적인 토크 조절이 필요하다. The purpose of this study was to evaluate the stress distributions at the periodontal ligament (PDL) and displacements of the maxillary first molar when mesially directed force was applied under various molar angulations and rotations. A three dimensional finite element model of the maxillary first molar and its periodontal ligament was made. Upright position, mesially angulated position by 20˚ and distally angulated position of the same degree were simulated to investigate the effect of molar angulation. An anteriorly directed force of 200g, countertipping moment of 1,800gm-mm (9:1 moment/force ratio) and counterrotation moment of 1,000gm-mm (5:1 moment/force ratio) were applied In each situation. To evaluate the effect of molar rotation on the stress distribution, mesial-in rotation by 20˚ and the same amount of distal-in rotation were simulated. The same force and moments were applied in each situation. The results were as follows: In all situations, there was no significant difference in mesially directed tooth displacement. Also, any differences in stress distributions could not be found, in other words, there were no different mesial movements. Stress distributions and tooth displacement of the 20˚ mesially angulated situation were very similar with those of the 20° distal-in rotated situation. The same phenomenon was obserned between the 20° distally angulated situation and 20˚ mesial-In rotated situation. When the tooth was mesially angulated, or distal-in rotated, mesially directed force made the tooth rotate In the coronal plane, with its roots moving buccally, and Its crown moving lingually. When the tooth was distally angulated, or mesial-in rotated, mesially directed force made the tooth rotate in the coronal plane, with its roots moving lingually, and its crown moving buccally. When force Is applied to an angulated or rotated molar, the orthodontist should understand that additional torque control is needed to prevent unwanted tooth rotation in the coronal plane.