Hypoxia-Responsive MicroRNA-101 Promotes Angiogenesis <i>via</i> Heme Oxygenase-1/Vascular Endothelial Growth Factor Axis by Targeting Cullin 3

Kim, Ji-Hee,Lee, Kwang-Soon,Lee, Dong-Keon,Kim, Joohwan,Kwak, Su-Nam,Ha, Kwon-Soo,Choe, Jongseon,Won, Moo-Ho,Cho, Byung-Ryul,Jeoung, Dooil,Lee, Hansoo,Kwon, Young-Guen,Kim, Young-Myeong Mary Ann Liebert 2014 Antioxidants & redox signaling Vol.21 No.18

<P>Aims: Hypoxia induces expression of various genes and microRNAs (miRs) that regulate angiogenesis and vascular function. In this study, we investigated a new functional role of new hypoxia-responsive miR-101 in angiogenesis and its underlying mechanism for regulating heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) expression. Results: We found that hypoxia induced miR-101, which binds to the 3 ' untranslated region of cullin 3 (Cul3) and stabilizes nuclear factor erythroid-derived 2-related factor 2 (Nrf2) via inhibition of the proteasomal degradation pathway. miR-101 overexpression promoted Nrf2 nuclear accumulation, which was accompanied with increases in HO-1 induction, VEGF expression, and endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) production. The elevated NO-induced S-nitrosylation of Kelch-like ECH-associated protein 1 and subsequent induction of Nrf2-dependent HO-1 lead to further elevation of VEGF production via a positive feedback loop between the Nrf2/HO-1 and VEGF/eNOS axes. Moreover, miR-101 promoted angiogenic signals and angiogenesis both in vitro and in vivo, and these events were attenuated by inhibiting the biological activity of HO-1, VEGF, or eNOS. Moreover, these effects were also observed in aortic rings from HO-1(+/-) and eNOS(-/-) mice. Local overexpression of miR-101 improved therapeutic angiogenesis and perfusion recovery in the ischemic mouse hindlimb, whereas antagomiR-101 diminished regional blood flow. Innovation: Hypoxia-responsive miR-101 stimulates angiogenesis by activating the HO-1/VEGF/eNOS axis via Cul3 targeting. Thus, miR-101 is a novel angiomir. Conclusion: Our results provide new mechanistic insights into a functional role of miR-101 as a potential therapeutic target in angiogenesis and vascular remodeling. Antioxid. Redox Signal. 21, 2469-2482.</P>

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

형성평가에 대한 과학교사들의 인식 및 실태

南貞姬,成乙善,飮在鎬,金慶姬,崔秉舜 한국교원대학교 과학교육연구소 1999 청람과학교육연구논총 Vol.9 No.1

전국의 초, 중, 고등학교 과학교사 197명을 대상으로 과학교육에서 형성평가에 대한 인식 및 이에 대한 실태를 질문지를 이용하여 조사하였다. 교사들은 학교교육에서 평가의 가장 중요한 목적이 학습목표의 성취를 확인하기 위한 것이라고 생각하고 있었으며, 가장 적절한 평가방법은 형성평가라고 생각하고 있었다. 따라서 형성평가는 수업 후반부에 학습내용의 성취정도를 확인하는 평가라고 인식하고 있었다. 대부분의 교사들이 형성평가가 매우 필요하다고 인식하고 있었고, 그 이유로 학습목표 달성 정도의 확인을 들었다. 그러나 과학 수업 중 수시로 이루어지는 형성평가는 적었다. 형성평가의 실시를 어렵게 만드는 원인으로 학생 수 과다, 시간 부족, 준비의 어려움을 들었으며, 형성평가로부터 얻은 정보는 학생들의 성취수준을 판단하는데 이용한다고 응답하였다. The science teachers' perceptions and the status of formative assessment in science teaching were identified through a questionnaire. The majority of teachers thought that the most important goal of assessment in education was to identify to what extent pupils achieved learning objectives. They suggested that formative assessment was the most appropriate method regardless of the purposes of assessment. They thought that formative assessment was an assessment carried out at the end of lesson to identify to what extent pupils achieved learning objectives. The teachers stressed the need for formative assessment to identify what pupils achieved. But they carried out formative assessment about once a week and there were very few teachers carrying out formative assessment during the lesson. They responded that it was difficult to carry out formative assessment because of large class size, lack of time and difficulty in preparation. And they used the information from formative assessment to judge the level of attainment.

새마을運動의 繼承發展方向

高秉浩,南基旻,趙喆柱 청주대학교 새마을연구소 1990 새마을硏究論文集 Vol.7 No.-

Looking back upon the Saemaul Undong, even since it began in the year 1970, it has been developed more powerfully and accomplished community development with the slogan of diligence, self-help and cooperation. As the result, most villages have gotten the improvement of physical environment, income increasing and positive changes in the attitudes and values of inhabitants. However, in spite of the Saemaul Undong's achievements, the movement has been faced to many kind of problems about the middle of the time of 1980's. And so nowadays, Saemaul Undong is not get out of a swamp of stagnation. Of course, it cannot he denied that it has made much contribution to the community development, but it is confronted with many serious problems. The reasons for the decline of Saemaul Undong are mainly the corruption of the headquarters of Saemaul Undong, disordered society, disparity of strata, depersonalization, the lack of new methodologies in the pursuit of Saemaul Undong, and etc. Recognizing these problems and to cope with the newly emerzing situation, a need has arisen for a change in Saemaul Undong. Therefore, these paper aims at researching for a new methodologies and strategies to carry out Saemaul Undong's progams continuously, and intends to suggest a new direction of sustaining Saemaul Undong. For the Purposes of suggesting altternatives continuously sustaining Saemaul Undong, this paper intends to formulate the principles and new directions of Saemaul Undong as follows : (1) The objectives and methodologies of Saemaul Undong should be reorganized in the context of new emerging situations. (2) Revitalizing local towns and rural areas must be pursued with special reference with hometown development. For the purpose, it is proper to organize a society for the support of hometown, and to invest money and resources in trying to help it. (3) In the spiritual aspect, it is desirable that the spirits of Saemaul Undong : diligence, self-help and cooperation should be expanded into a public interest and self-governing. (4) To induce residents to take part in and support Saemaul Undong, Noluntary Systems are formulated for securing public interest and a cultivation of personality. (5) A public movement creating a new nation, to meet a new twenty-frist centry, should be pushed ahead with a nation-wide development. (6) In a dimension of province level Saemaul Undong, the local branch of the headquarters of Saemaul Undong must take the initiative in doing Saemaul projects, and residents show their ability to carry out Saemaul Undong.

초음파 어레이를 이용한 이동 로봇의 장애물 회피에 관한 연구

김병남,권오상,김기호,이응혁 建陽大學校 1999 建陽論叢 Vol.- No.7

For mobile robot, the navigation effectiveness can be improved by providing autonomy, but this autonomy requires the mobile robot to detect unknown obstacles and avoid collisions while moving it toward the target. This paper presents an effective method for autonomous navigation of the mobile robot in structured environments. This method uses ultrasonic sensor array to detect obstacles and utilizes force relationship between the obstacles and target for avoiding collisions. Accuracy of sensory data produced by ultrasonic sensors is improved by employing error eliminating rapid ultrasonic firing (EERUF) technique. Navigation algorithm controlling both the velocity and steering simultaneously is developed, implemented to the mobile robot and tested on the floor filled with the cluttered obstacles. It is verified that from the results of the field tests the mobile robot can move at a maximum speed of 0.66 m/sec without any collisions.

초등학교 과학수업에서 형성평가의 실제

엄재호,남정희,최병순 한국초등과학교육학회 2000 초등과학교육 Vol.19 No.2

The purpose of this study was to investigate the characteristics of formative assessment in elementary school science teaching. In order to examine the practices of formative assessment in science teaching, 8 science classes were observed and video-taped for each of two instructions. We also interviewed the teachers and students with semi-structured questions. The result indicated that the teachers used planned formative assessment and interactive formative assessment. Teachers assessed three aspects of student learning in science classroom: the student's personal, social and science development. However, the majority assessed in science teaching was science development. Teachers used observation, question and answer, dialogue, reports, and presentation as the formative assessment methods. The process of formative assessment was categorized as to get information, to judge and to give feedback. These three aspects were interrelated and interdependent. The type of question and feedback was influenced on the extent of the interaction between teachers and students.

수평배수재 배열의 진공압밀효과에 대한 수치해석

박병수,정길수,이종호,유남재 강원대학교 산업기술연구소 2003 産業技術硏究 Vol.23 No.A

This paper is results of numerical works of investigating effects of horizontal drain arrangements on vacuum consolidation. Extensive numerical analyses were carried out to find the appropriate arrangements of horizontal dram of vacuum consolidation. Commercially available program of CRISP. well know-n to be good to modelling the behavior of clay- material, was used Cam-clay model, based on the Critical State of Soil Mechanics(CSSM), was used to simulate the geotechnical engineering behavior of clay. Model test results carried out previously in the laboratory w-ere compared with numerically estimated results and it was found that results about consolidational settlement with times were in good agreements. Based on this confirmation. parametric numerical study was performed to investigate effects of horizontal drain arrangements on vacuum consolidation with changing the vertical and horizontal spacings between drains for the given soil properties and vacuum. The effect of distance of drain located in top layer from the surface of the ground on the settlement due to vacuum was also investigated. As a results of numerical analyses. appropriate arrangements of horizontal drain to maximize the consolidation settlement due to vacuum were found. The mechanism of vacuum consolidation about the vacuum pressures being transferred to the effective stresses around drains was also evaluated.

성견에서 교정적 고정원으로서의 티타늄 미니스크류에 대한 연구 : An experimental investigation in dogs

윤병수,최병호,이원유,김경남,심형보,박진형 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.5

교정치료에서 원하는 치아이동을 위해서는 안정된 고정원이 필요한데 티타늄 미니스크류가 매식과 제거가 쉽고, 구강 내 여러 부위에서 적용이 가능하고, 환자가 느끼는 불편감이나 비용적인 부담이 적고, 제거 후에 치유가 빠르게 진행될 수 있는 등의 장점이 있어 최근에 교정적 고정원으로 사용되기 시작하였다. 티타늄 미니스크류를 교정적 고정원으로 사용한 임상 예들이 여러 편 발표되었는데 미니스크류의 이완이 가장 큰 실패의 원인으로 보고되고 있다. 그러나 지금까지 보고된 논문들에서 교정적 고정원으로 안정성을 줄 수 있는 스크류의 식립 길이에 관한 연구가 없는 상태이다. 교정적 고정원으로 미니스크류를 효과적으로 사용하기 위해서는 식립 부위에 따른 골구조와 골밀도 차이를 고려한 식립 길이에 관한 기준이 필요하다. 이에 본 연구에서는 성견의 상악골과 하악골에서 직경 2mm 티타늄 미니스크류를 다양한 길이로 식립하고 교정력을 적용한 후 그 안정성을 평가하여 교정적 고정원으로 사용될 수 있는 미니스크류의 식립 길이를 결정하고자 하였다. 미니스크류가 상악에서는 6mm이상, 하악에서는 4mm 이상이 골 내에 식립될 때 8주 동안 200g의 교정력에 동요도나 위치변화를 보이지 않았다. 식립 부위로는 부착치은 부위 치근 사이에 식립될 때 구강청결이 유지되고 미니스크류 주변 치은조직에 자극을 주지 않아 정상적인 조직으로 유지 될 수 있었다. 또한 교정력 적용 8주 후 치근단 방사선 사진검사에서 스크류 주변 치근 흡수나 치조골 흡수, 치주 인대 손상이 관찰되지 않았다. 따라서 상 ㆍ 하악 골밀도와 골구조의 차이를 고려하여 미니스크류의 골내 식립 길이를 적절히 조절함으로써 교정적 고정원으로 티타늄 미니스크류가 효과적으로 사용될 수 있다고 생각된다. Titanium miniscrews are being used increasingly as an anchorage for tooth movement, because they are easy to place and to remove, increase the number of sites available, give minimum strain to patients regarding surgical procedures, and offer uneventful healing after removal. The use of titanium miniscrews as an orthodontic anchorage has been reported in clinical case reports, but clinicians hove experienced screw loosening when using such screws. To our knowledge, there are no published reports evaluating the stability of miniscrews. Information about the length of miniscrews used in relation to the location is of some importance, as stability will vary depending on bone quality. The purpose of this study was to evaluate a variety of lengths of miniscrews (diameter: 2mm) which were inserted in maxilla or mandible and to demonstrate in a dog moedl which miniscrew provides fundamental stability in the jaws. 10 mm long miniscrews in the maxilla and 8mm long miniscrews in the mandible showed no clinical mobility and retained their position throughout an 8 weeks force (200g) application. The mucosal condition around the screws was healthy in cases in which miniscrews were inserted in the alveolar bone between the roots and the head of the screws emerged into the attached gingiva. When the force application was terminated, radiographic analysis revealed neither root resorption nor periodontal pathology around the miniscrews that remained stable during the entire treatment period. This study suggests that if titanium miniscrews with adequate length are properly used depending on the location, they provide sufficient stability for orthodontic anchorage.

花粉形態 및 유리 아미노산의 성분에 의한 소나무속의 分類

辛昌男,柳柄泰,柳成浩 충남대학교 자연과학연구소 1983 忠南科學硏究誌 Vol.10 No.2

3 species of Pinus in Korea, P. densiflora, P. rigida, P. koraiensis, were classified by polynological studies and TLC analysis of the free amino acids in pollen. The shapes of pollen grain are similar in 3 species. P. densiflora showed some differences in the size of pollen grain and in the number, the pattern, the angle of attachment and the size of air bladder with other 2 species (p<0.05). The cell wall thickness and the type of germ furrow surface of P. koraiensis are different from those of P. densiflora and P. rigida (p<0.05). Serine, glutamic acid, alanine, valine and histidine were detected in the pollen of P.densiflora and P. rigida, but not in that of P. koraiensis.

체내 납부담의 지표로서 누적 혈중 납량과 누적 혈중 ZPP가 납 관련 신기능에 미치는 영향

윤강호,김남수,김진호,김화성,이병국 대한산업의학회 2006 대한직업환경의학회지 Vol.18 No.4

목적: 체내 납부담의 지표로서 누적 혈중 납량과 누적 혈중 ZPP가 납관련 신기능에 미치는 영향을 알아보기 위하여 678명의 납 작업자들을 대상으로 본 연구를 시도하였다. 방법: 누적 혈중 납량과 누적 혈중 ZPP는 입사시부터 매 1년간의 혈중 납량과 혈중 ZPP를 누적하여 구하였다. 입사시기가 1983년 이전인 납 작업자의 경우는 최초 혈중 납량과 최초 혈중 ZPP를 그 이전의 값으로 추정하여 구하였다. 체내 납부담의 지표로서 골중 납량과 DMSA착화납량을 측정하였으며, 최근의 납 노출 지표로서는 혈중 납량과 혈중 ZPP를 측정하였다. 신기능을 평가하는 지표로서는 임상 신기능 지표로서 BUN과 혈청 creatinine을 측정하였고, 신기능 조기지표로는 NAG (N-acetyl-D- glucosamide)와 RBP (Retinol binding protein)를 측정하였다. 결과: 혈중 납량과 혈중 ZPP의 누적지수와 골중 납량과는 결정계수(r²)는 각각 0.72와 0.567로서 유의한 상관이 있었으며 이들 누적지수와 골중 납량은 곡선형적 모델이 더 적합하였다. 신기능 지표들을 종속변수로 하고 성, 연령, 직력 및 음주와 흡연을 통제한 후 혈중 납량과 혈중 ZPP를 독립변수로 한 중회귀분석에서 혈중 납량은 4개의 신기능 지표들 중 지수 변환한 NAG와만 유의한 관련이 있는 반면, 혈중 ZPP는 지수변환한 NAG를 제외한 신기능 지표들과 유의한 관련이 있었다. 한편 신기능 지표들을 종속변수로 하고 연령 및 직력과 음주와 흡연을 통제한 후 체내 납 부담노출지표들을 독립변수로 한 중회귀분석에서 골중 납량과 혈중 ZPP 누적지수는 4개의 신기능 지표들과 유의한 관련이 있는 반면, DMSA 착화납량과 혈중 납량 누적지수는 BUN을 제외한 신기능 지표들과 유의한 관련이 있었다. 결론: 상기한 결과로 보아 혈중 납량과 혈중 ZPP의 누적지수는 체내 납부담의 지표로서 효용성이 입증되었으며, 특히 혈중 ZPP의 누적지수가 혈중 납량의 누적지수보다 체내 납부담의 대리지표로서 더 좋은 것으로 나타났다. Objective: To evaluate the possibility of cumulative blood lead and blood ZPP as surrogates of lead body burden and to investigate their association with renal function as an index of lead body burden. Methods: The study subjects comprised 678 lead workers with past blood lead and blood ZPP data from their employment. Cumulative blood and ZPP were calculated by accumulating the every year mean value of both indices from the new employment since 1983. To assess the cumulative data of lead workers who started their lead work before 1983, the years before 1983 were simulated with the first available data from 1983. Study variables for lead body burden were tibia bone lead and DMSA chelatable lead, whereas those for current lead biomarkers were blood lead and blood ZPP. BUN and serum creatinine were selected as clinical renal biomarkers, while NAG (N-acetyl-D-glucosamine) and RBP (Retinol binding protein) were selected as early renal biomarkers. Results: The association between cumulative blood lead and blood ZPP with tibia bone lead was statistically significant with determinant coefficients (r²) of 0.72 and 0.567, respectively, and their relationships were better explained by the curvilinear regression model. In multiple regression analysis of current lead biomarkers on the renal biomarkers after controlling for possible confounders (age, sex, job duration, smoking and drinking status), blood lead was associated only with log-transformed NAG, whereas blood ZPP was associated with 3 other renal biomarkers. On the other hand, in multiple regression analysis of biomarkers of lead body burden on renal biomarkers after controlling for possible confounders (age, sex, job duration, smoking and drinking status), cumulative blood ZPP and tibia bone lead were associated with all 4 renal function biomarkers, whereas cumulative blood lead and DMSA chelatable lead were associated with 3 renal biomarkers except BUN. Conclusion: Cumulative blood and ZPP were demonstrated to be good surrogates of lead burden. Furthermore, the cumulative blood ZPP was confirmed to have a better association than the cumulative blood lead.