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李炳基,殷鍾旋,高正愛,韓光洙 전북대학교 유전공학연구소 1988 遺傳工學硏究所報 Vol.1 No.1
This study was carried out to investigate the optimal conditions for producing somatic cell hybrids of vegetables. The conditions investigated are enzyme concentrations and duration of treatment for isolation of protoplast, concentration of PEG 4000 for fusion of isolated protoplasts, and viability of isolated protoplasts and fused protoplasts. The results obtained are as follows: 1. The size of onion protoplasts which are isolated from white mesophyll tissue is 52.6μm. The size of welsh onion and nanking shallot protoplasts is almost uniform (about 45μm) but the size of tomato and potato protoplasts isolated from callus tissue is various(21 to 86μm). 2. The optimal conc. of enzyme for isolation of protoplasts in the mesophyll tissue of radish, chinese cabbage, welsh onionm, and nanking ahallot are 0.5% macerozyme and 1.0% cellulase. While the onion is 0.5% macerozyme and 1.5% cellulase. 3. The optimal duration of enzyme treatment for produced intact protoplasts were 4hr in chinese cabbage, 5hr in radish, 3hr in welsh onion, and 2hr in nanking shallot and onion respectively. 4. Isolated protoplasts from mesophyll tissus were fused easily and produced the hetero-karyon with 25-35% PEG 4000 in inter-or intraspecies, while protoplasts of tomato and potato derived from cell were not fused easily. 5. The isolated protoplasts were viable until 1-2 days duration of culture, after 3-4 days they were died. The fused protoplasts regenerate cell wall in 1-2 days, while cell division and morphological changes were not observed.
오리엔탈나리의 球根 收穫時期와 低溫處理 期間이 生育에 미치는 影響
金熙峻,金正萬,金致善,柳汀,崔泳根,文炳永 한국화훼연구회 2001 화훼연구 Vol.9 No.1
This study was carried out to determine the effect of the duration of chilling treatment and the lifting time of bulbs on growth of Lilium Oriental hybrids for product of cut flower in the type of forcing or semiforcing culture in Korea. "Casablanca", "Acapulco" and "Le Reve" in lilium Oriental hybrids were used in these experiment and carried out from 1999 to 2000 in Iksan, Korea. The bulbs for experiment were lifted on August 27, September 27 and October 27 and the all of them were chilled at 5±1℃ for 0,8,10,12 weeks. Development of the bulbs lifted on October 27 was superior than that of lifted bulbs on August 27 in the three cultivars. The bulbs lilted lately and chilled for a long time were sprouted more earlier and grown faster. In order to increase bulb sprouting until 100%, the duration of chilling treatment of bulbs was need for 12 weeks on August 27, 10 weeks on September 27 and above 8 weeks on October 27. The flowering according to the duration of chilling treatment and the lifting time of bulbs was same above. Consequently, the bulbs should be chilled at 5±1℃ for chilled for 10 weeks at late September and 8 weeks in producted bulbs at late October in earlyㆍMidseason variety fur product of cut flower hut bulbs of late variety should be chilled for 12 weeks at late September and 10 weeks at late October.
Jeong, Byeong-Seon,Choi, Ho-Young,Kwak, Young-Shin,Lee, Eung-Seok Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.10
A series of 2,4,6-tripyridyl pyridines were synthesized, and evaluated for their antitumor cytotoxicity, topoisomerase I and II inhibitory activity. From the eighteen prepared compounds, compounds 10-12 have shown better or similar cytotoxicity against several human cancer cell lines as compared to 2,2':6',2"-terpyridine and doxorubicin. Especially, compound 10 exhibited the most potent cytotoxicity better than positive controls. Structure-activity relationship study indicated that 2,2':6',2"-terpyridine skeleton has an important role in displaying significant cytotoxicity against several human cancer cell lines.
( Byeong Seon Jeong ),( Hoyoung Choi ),( Young Shin Kwak ),( Eung Seok Lee ) 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0
A series of 2,4,6-tripyridyl pyridines were synthesized, and evaluated for their antitumor cytotoxicity,topoisomerase I and II inhibitory activity. From the eighteen prepared compounds, compounds 10-12 have shown better or similar cytotoxicity against several human cancer cell lines as compared to 2,2`:6`,2``-terpyridine and doxorubicin. Especially, compound 10 exhibited the most potent cytotoxicity better than positive controls. Structure-activity relationship study indicated that 2,2`:6`,2``-terpyridine skeleton has an important role in displaying significant cytotoxicity against several human cancer cell lines.
Modification of C2 Functional Group on. Asiatic Acid and the Evaluation of Hepatoprotective Effects
Jeong, Byeong-Seon,Lee, Mi-Kyeong,Kim, Young-Choong,Lee, Eung-Seok 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3
For the development of novel hepatoprotective agents, C2 functional group on asiatic acid was modified, and evaluated for their hepatoprotective effects. Among prepared compounds, com-pounds 10 and 14 showed better hepatoprotective effects compared to silymarin.
Jeong, Byeong-Seon,Wang, Qian,Son, Jong-Keun,Jahng, Yurngdong WILEY-VCH Verlag 2007 EUROPEAN JOURNAL OF ORGANIC CHEMISTRY Vol.2007 No.8
<P>A versatile method for the total synthesis of cyclic diphenyl ether-type diarylheptanoids, acerogenin C, acerogenin L, (±)-galeon, and (±)-pterocarine was described. The Ullmann reaction of suitably substituted linear diphenylheptanoids was employed for the intramolecular formation of the key ether intermediates as the final step. The prerequisite diarylheptanoids were prepared by a series of cross-aldol condensation reactions from readily available starting benzaldehydes.(© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)</P> <B>Graphic Abstract</B> <P> <img src='wiley_img/1434193X-2007-2007-8-EJOC200600938-fig000.gif' alt='wiley_img/1434193X-2007-2007-8-EJOC200600938-fig000'> </P>
Jeong, Byeong-Seon,Kim, Young-Choong,Lee, Eung-Seok Korean Chemical Society 2007 Bulletin of the Korean Chemical Society Vol.28 No.6
For the development of novel hepatoprotective agents, C2, C3, C23 and C28 functional groups on asiatic acid were modified, and the prepared compounds were evaluated for their hepatoprotective effects. Among the prepared compounds, 9, 13 and 16 showed significant hepatoprotective activities against CCl4- and galactosamine (GaIN)-induced hepatotoxicity. Especially, compound 9 showed the most significant hepatoprotective effects against GaIN-induced hepatotoxicity (66.4% protection at 50 μM) and moderate hepatoprotective activities against CCl4-induced hepatotoxicity (20.7% protection at 50 μM).