도토리 추출물의 3T3-L1 세포 분화억제 효과

김지연,이진,이창원,김애정,Kim, Ji-Yeon,Lee, Jin,Lee, Chang-Won,Kim, Ae-Jung 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

본 연구에서는 만성질환의 주요 위험인자인 비만을 예방하기 위한 식품소재로서 3T3-L1세포를 이용하여 탈피한 도토리의 항비만 효과를 알아보고자 하였다. 3T3-L1 세포에서 생존율(MTT assay)을 측정한 결과, AE와 AW 시료 모두 $500{\mu}g/mL$ 농도에서는 다소 생존율의 감소를 보여, $300{\mu}g/mL$를 최종 농도로 정하였다. 3T3-L1 세포의 지질축적 억제 효과를 측정한 결과, 농도 $100{\mu}g/mL$로 처리하였을 때 두 시료 모두 지질축적량의 증가를 보였으나, $200{\mu}g/mL$ 처리농도에서 AE 시료는 82%로, AW 시료는 74%로 감소되다가 $300 {\mu}g/mL$ 농도에서는 두 시료 모두 약 53% 수준까지 지질축적이 억제되었다. 3T3-L1 세포에서 중성지방 억제 효과를 확인한 결과, AE 시료의 경우 $200{\mu}g/mL$ 농도에서 11%의 감소율, $300{\mu}g/mL$ 농도에서 42% 수준의 감소율을 보였다. AW 시료도 $200{\mu}g/mL$ 농도에서 5%의 감소율과 $300{\mu}g/mL$에서 41%의 감소율을 보였다. 3T3-L1 세포의 ROS 생성량을 측정한 결과, 시료 농도 $200{\mu}g/mL$에서 AE는 42%, AW는 33%로 $300{\mu}g/mL$에서는 AE는 58%, AW는 52%로 ROS 생성량의 억제를 보였다. 3T3-L1 세포에서 mRNA 발현에 미치는 영향을 대조군과 비교하였을 때 두 시료(AE와 AW) 모두 $300{\mu}g/mL$ 농도에서 $PPAR-{\gamma}$은 54%와 38%, aP2는 40%와 18% 수준의 발현을 억제시키는 것으로 나타났다. 이상의 결과로 볼 때 탈피한 도토리는 3T3-L1 세포의 분화를 억제함으로써 새로운 항비만 소재로의 가능성이 있는 것으로 판단된다. This study aimed to investigate the suppressive effect of acorn extracts, by evaluating 70% ethanol extract (AE) and hot water extract (AW) using 3T3-L1 preadipocytes. We applied various levels (0, 100, 200, 300 and $500 {\mu}g/mL$) of AE and AW to 3T3-L1 preadipocytes. The cell viability of the 3T3-L1 preadipocytes was not affected by up $300 {\mu}g/mL$ of extracts, but was suppressed by level $500{\mu}g/mL$ of both AE and AW by 20% and 9% respectively. The accumulation of lipid droplets in differentiated 3T3-L1 preadipocytes was dose-dependently suppressed by AE and AW. Especially, at high concentrations ($300{\mu}g/mL$), AE (42%) was more effective than AW (41%). Reactive oxygen species (ROS) was also dose-dependently suppressed by treatment with AE (58%) and AW (52%). With regard to the mRNA related to differentiated 3T3-L1 preadipocytes, $PPAR-{\gamma}$ and aP2 were suppressed by treatment with AE (54 and 40%) and AW (38 and 18%). From our results, acorn extract (AE) has more suppressive effects than AW in differentiated 3T3-L1 preadipocytes. We therefore concluded that acorn has suppressive effects against obesity in differentiated 3T3-L1 cells due to antioxidation.

Acute exacerbation of interstitial lung disease; subgroup analysis for causes, risk factors for mortality rate, and steroid responsiveness

( Seung Hyun Yong ),( Ah Young Leem ),( Song Yee Kim ),( Kyung Soo Chung ),( Ji Ye Jung ),( Young Ae Kang ),( Young Sam Kim ),( Joon Chang ),( Moo Suk Park ),( Hye Jin Jang ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-

Background: Interstitial Lung Disease (ILD) has known as a poor prognosis disease, particularly idiopathic pulmonary fibrosis (IPF). Corticosteroid is widely used for treatment of acute exacerbation (AE)-ILD. This study aims to clarify efficacy of corticosteroid use, and compare the mortality rate depending on the subgroups of ILD. Materials and Methods: We collected the data from the patients who have diagnosed ILD and visited emergency room (ER) with the respiratory symptoms from January 1, 2016 to December 31, 2018. We classified AE-ILD patients into 3 groups depending on methylprednisolone dose per kilogram. Also, we compared the mortality of IPF patients to that of non-IPF patients. Results: A total case of AE-ILD visited ER from 2016 to 2018 was 209 cases, including IPF cases (n=122) and non-IPF cases (n=87). We divided total patients (n=209) into three groups depending on steroid use, and these include group 1 of 0-0.49 mg/kg (n=78), group 2 of 0.5-0.99 mg/kg (n=58), and above 1.0 mg/kg (n=41), each. We found poor prognosis from the cases of IPF compared to non-IPF cases (p=0.029, OR 2.463). Furthermore, there was significant differences of mortality rate according to steroid dose (p=0.036, OR 1.712), CRP elevation (p=0.016, OR 1.004), and intubated case (p=0.000, OR 8.152). Also, we categorized IPF (n=122) to non-IPF cases (n=87) and analyzed the mortality rate. Most of the patients were death from ILD itself (n=23, 63.9%), Pneumonia(n=8, 22.2%), and Lung cancer (n=5, 13.9%). Bacterial infection (n=26) was dominant cause of AE, followed by fungal infection (n=15), and viral infection (n=10). Conclusions: This retrospective analysis of corticosteroid treatment shows more fatal outcome when we use high dose steroid in AE-ILD cases, and there was significant difference in mortality rate depending on the subgroup of ILD and medical background, such as intubation status, higher CRP level, and IPF cases.

과학적 근거 중심 평가를 도입한 마늘의 콜레스테롤 조절 기능성 평가

김인혜;조강진;김진영;황경아;황유진;채유진;엄애선 한양대학교 2010 韓國 生活 科學 硏究 Vol.30 No.1

Garlic (Allium sativum) is used a flavouring agent and a traditional medicine in human history. Numerous studies support the fact that garlic intake may have effect on cholesetrol-lowering. No claims currently have been made about garlic intake and cholesterol-lowering effect with respect to health claim in Korea. The aim of this study was to evaluate the scientific evidence on garlic intake and cholesterol-lowering effect by the scientific evidence-based rating system of Korean Food and Drug Administration for health claim. We carried out a comprehensive search of the Pubmed, EMBASE, Cochrane Library and manual searches of relevant journals for studies between 1955-2009. The search terms were garlic and Allium sativum in combination with cholesterol. Eighteen clinical stidies were included and reviewed to evaluate the strength of evidence that supports a relation between garlic intake and cholesterol-lowering effect. Our results suggests that level of scientific evidence on garlic intake and cholesterol-lowering effect is "other function (II) probable".

간호 학생의 임상실습 효율을 위한 기초 조사 연구

이애경,김정애,주미경,정안순,배경진,김정수,이정애,장은정 경복대학 2000 京福論叢 Vol.4 No.-

임상실습 교육은 간호이론을 실무에 적용하는 기회를 제공하는 과정으로서 임상실습이 간호교육의 중요한비중을 차지하고 있다는 것은 주지의 사실이다. 이러한 중요성에도 불구하고, 임상실습 교육에 즉각적인 해결이 어려운 많은 문제점이 있음도 이미 알고 있는 사실이다 이에 임상실습 교육의 기초 자료로 활용되고 있는 실습목록표에 대한 간호 학생들의 실습목표의 명확한 이해와 더불어 이론과 실습의 연계성을 강화시키고자 하였다. 성인간호, 모성간호, 이동간호 3개 영역 모두에서 공통적으로 기본임상간호 항목이나 기본간호술과 관련된 항목에서 직접수행의 빈도가 높았고 숙련된 기술적인 처치나 전문성을 필요로 하는 항목에서는 관찰과 수행경험이 부족한 것으로 나타났다. Clinical performance is not only the process of providing the opportunity for applying nursing theory into practice but also it is a well-known fact that clinical performance is an important part in nursing education. lnspite of this importance, it is also true that many problem with no immediate solution exist in clinical performance of nursing students. thus, through measuring and analyzing the degree of clinical experience of the nursing students, on the nursing checklist that is being used as the basic guide in clinical education, we tried to clearly understand the objectives of clinical performance and to emphasize the connection between theory and clinical performance. The results of the study showed that the frequency of directly performing tasks was high in all areas of adult health nursing, maternity nursing, and child nursing for the items of basics clinical nursing and items related with basic nursing techniques, and that the experiences of observation and execution were lacking in the items needing skilled treatment or speciality.

해조류를 이용한 항생재 대체 사료 첨가제 개발

박진용,김진욱,이희현,이송애,김진욱,이재화 신라대학교 자연과학연구소 2005 自然科學論文集 Vol.14 No.-

우리나라의 항생제 남용 문제는 세계적으로 심각 하다고 한다. 이러한 문제를 해결하기 위해서 정부는 다양한 정책을 도입하고 있으며 특히 축산물의 항생제 남용 문제를 해결하기위해 사료 관리법을 강화하여 항생제 사용을 억제하고 있어 항생제를 대체 할 물질이 필요하다. 이런 문제를 해결하기 위해서 우리나라의 다양한 해조류를 이용한 항생제 개발을 통해서 항생제 남용 문제를 해결하고 소비자들에게 믿을 수 있는 축산물을 제공 할 것으로 보인다. Feed official law was strengthened recently. The antibiotic use is suppressed with feed official law reinforcement. The antibiotic transfer material is necessary. To use the seaweeds and transfer the antibiotic. It is a possibility of a high income. This is new bio industry. This the goods production which is competitive power is possible. It is a possibility of eating the livestock where also the consumers are good.

The level of urinary aflatoxin M1 in Korean adults

Yong-Dae Kim1, Hyojin Kwon, Sun-In Moon, Sang-Yong Eom, Jung-Duk Park, Byung-Sun Choi, Seok-Joon Sohn, Young-Seoub Hong, Ho Kim, Ho-Jang Kwon, Ji-Ae Lim, Hae-Jung Yoon, Gwang-Jin Kim, Heon Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Competitive ELISA methods were used to measure the level of aflatoxin M1 (AFM1) from urine in 1008 Korean adults. Subjects were selected by random sampling in all areas of Korea, except Cheju-do. The recovery rate of AFM1 using this method was 105% (73-124%). The geometric mean of urinary AFM1 in all subjects was 3.43 pg/mL (3.67 ng/g creatinine). The level of AFM1 in males was statistically higher, compared with female subjects. However, the levels of AFM1 did not differ according to age. Subjects in Chungbuk-do showed the highest urinary AFM1 concentration, whereas subjects in Kyeongnam-do showed the lowest concentration. Assuming an excretion rate of 5%, this AFM1 excretion corresponds to approximately 0.1 microgram/day in Korean adults.

국내육성 오차드그라스 품종들의 캘러스 형성율 및 식물체 재분화 효율

김기용,강경민,배은경,이인애,임용우,최기준,박근제,손대영,조진기 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Comparisons of callus formation ratios from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency for 4 orchardgrass (Dactylis glomerata L.) varieties (Three were developed in Korea and one was imported from foreign nation) are as follows; Jangbeol 102 (67.0%) has the highest callus formation ratio in 4 weeks incubated callus after bedding the seed explants, but Potomac (68.4%) has the highest ratio in 6-seek callus. Potomac (3.93cm) has the highest callus size in 4-week callus, but Jangbeol 101 (4.32cm) has the highest size in 6-week callus. Jangbeol 101 (17.7%) has the highest plant regeneration ratio in 4-week callus, but Potomac (37.4%) has the highest raion in 6-week callus. Jangbeol 102 (11.5%) has the highest plant regeneration efficiency in 4-week callus, but Potomac (25.6%) has the highest efficiency in 6-week callus.

매일신보광고를 통해 본 일제시대 한국복식의 변천

金鎭玖,金愛蓮 服飾文化學會 1999 服飾文化硏究 Vol.7 No.2

The purpose of this study is to classify frequency of advertisement and types of advertisement by items and to analyze a primary factors were reflected in the costumes by a policy of rule under the Japanese rule. Data was MaIl Shin Bo's advertisement connected with costumes from 1910 to 1945 years. The results are as follows: 1. The order of the advertisement's frequency was footwear, cosmetics, soap, headgear, western style clothes, precious metals and so on. 2. The type of the advertisement was a format that transmits informs in all items. Cosmetics, hairdye and shampoo applied positive appeals. 3. In the military government, the advertisement's frequency connected with costumes was 37.5% and a shoe store was the first order. A shoes and headgear were high level, because of these were essential imports and were allowded as a proper articles for a western style clothes by a civilized policy. In the political periods of civilization, the advertisement's frequency connected with costumes was 54%. This result indicates industrial development of this period. Soap was the first order during 1924~1933 and cosmetics was the first order during 1934~1940. High level of the advertisement's frequency in these imports were reflected by a cultural policy as a link of an appeasement measure. In the political periods of a racial liquidation, the advertisement's frequency connected with costumes was 8.5% and the advertisement's order by items was cosmetics, a shoe store. The reason was that reflected the phases of the times that was serious by a shortage of goods and an reinforcement of wartime's attitudes throughout war.

일부 지방대학 흡연 남자대학생과 비흡연 남자대학생의 혈청 및 식이 Ca, P섭취 수준 및 혈압의 비교 연구

김애정,박수진,이혜인 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.2

This study was performed to observe the levels of serum and dietary Ca and P, and blood pressure in rural university male students(smoker: 35 persons, non smoker: 32 persons). Three-day dietary record and blood samples were collected for measurements of the Ca and P levels of dietary intake and serum. The results were as follows : 1) There were no significant differences between smokers and non-smokers in terms of height, weight, and BMI. 2) Mean blood pressures on smokers and non-smokers were 131.33/93.75㎜Hg, 119.37/80. 62㎜Hg, respectively. Blood pressure of smokers was higher than that of non-smokers(p<0.05). 3) There were no significant difference between smokers and non-smokers in calcium, phosphorus, and Ca/P ratio of dietary intake and serum. The results of this study suggest that non-smoking education programs for smokers including the information on the desirable food habits for prevention of hypertension should be developed.

박물관 브랜드 마케팅 및 공간 프로모션 전략 -숙명여자대학교 내 정영양자수박물관 C·E·M 공간디자인을 중심으로-

김은애,김예은,정채림,이진민,장미정,이성애 숙명여자대학교 산업디자인연구소 2017 숙명디자인학 연구 Vol.24 No.-

본 연구는 정영양자수박물관의 콘텐츠를 활용한 통합 브랜드를 개발함에 있어 브랜드·디자인 ·마케팅을 융합한 시점에서 바라보고 그래픽아이덴티티, 제품아이덴티티, 공간아이덴티티 통합브랜드를 구축하는 일련의 프로세스를 밝히는데 목적이 있다. 이에 본 연구는 기존의 박물관 방문자들과 20-30대 여성을 대상으로, 디자인과 마케팅의 융합된 측면에서 도출된 ‘C·E·M’을 物, 事, 人, 場가 융합된 문화로 한정한다. 그 결과, ‘C·E·M’은 디자인과 마케팅 전략을 융합하여 로고 및 사인물의 그래픽디자인부터 제품디자인, 공간디자인에 이르기까지 일관성과 객관성, 스토리성을 도출한다. 끝으로 본 연구는 브랜드가 디자인과 마케팅을 융합하여 더 체계화된 G·I, P·I, S·I의 통합 브랜드 매뉴얼을 만든 것에 의의가 있다. 아울러 향후 이와 관련된 통합브랜드 디자인 개발에 있어서 기초 자료로 활용될 것으로 기대된다