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Characterization and genetic mapping of white spotted leaf (wspl) mutant in rice
김백기,이혜림,Zhuo Jin,이동령,고희종 한국육종학회 2019 Plant Breeding and Biotechnology Vol.7 No.4
Spotted leaf mutants which produce necrotic lesions spontaneously are important sources to study programmed celldeath in plant defense responses. A novel white-spotted leaf (wspl) mutant was induced from Ilpum, Korean japonica rice cultivar bythe treatment of ethyl methane sulfonate (EMS). The phenotype of wspl mutant differed from that of other spotted leaf mutants in thatnot only brown spots but also white lesion mimic spots were observed on the tip of the leaves from the vegetative stage. Strong NBT andDAB staining were observed on the older leaf of wspl mutant in microscopic ROS assay, and the chlorophyll content of wspl mutantmaintained longer than wild-type in the old leaves. Genetic analysis revealed that the wspl mutant trait was controlled by a singlerecessive gene and the locus of wspl gene was mapped on the long arm of chromosome 5 between the flanking markers S05100 andS05112 (4.1 Mb). Through the combination of the genetic mapping and SNP analysis, two candidate genes for white-spotted leaf wereidentified in the genic region. A novel phenotype of white-spotted leaf mutant has not yet been reported, thus further study of the wsplmutant will contribute to understanding of the molecular mechanisms involved in lesion mimic phenotype in rice.