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Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells
Peralta‑Vega Adriana‑Berenice,Parra‑Barrera Alberto,Ramos‑Godínez María del Pilar,López‑Marure Rebeca,Arellano‑Galindo José,Gisela Gutiérrez‑Iglesias 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3
Background Titanium dioxide ( TiO2) is employed in the production of cosmetics, sunscreen, food, and drugs; however, TiO2 is toxic at the nanometric scale. Objective To analyze the in vitro toxic effect of TiO2 NPs on mesenchymal stem cells (hMSCs). Methods The hMSCs from Wharton`s jelly were exposed to 4, 62.5 and 500 μg/mL of TiO2 NPs. Viability and cell proliferation tests were carried out at 2, 5 and 7 days of exposure. Additionally, the osteogenic and adipogenic differentiation capacity was evaluated. Results TiO2 NPs had a perinuclear arrangement and internalized in vesicles into cytosol, diminished significantly the viability (40% and 30% and 30%) and the proliferation cellular (35%, 50% and 55%), differentiation to osteoblast (38%) and adipoblasts (20%). Conclusion TiO2 NPs affect viability, proliferation, and differentiation of hMSCs. Our result suggests more regulation of the use of TiO2.
Surgical management with intentional replantation on a tooth with palato-radicular groove
Jorge Forero-López,Luis Gamboa-Martínez,Laura Pico-Porras,Javier Laureano Niño-Barrera 대한치과보존학회 2015 Restorative Dentistry & Endodontics Vol.40 No.2
A palato-radicular groove (PRG) is a developmental anomaly primarily found in the maxillary lateral incisors. It is a potential communication path between the root canal and the periodontium that decreases the survival prognosis of the affected tooth, therefore compromising the stability of the dental structure in the oral cavity. The aim of this case report is to present an original technique where a PRG was treated by means of intracanal disinfection, PRG sealing with glass ionomer, replantation with intentional horizontal 180 degree rotation of the tooth, and an aesthetic veneer placed to provide adequate tooth morphology. The clinical and biological benefits of this novel technique are presented and discussed.
Laura Iztacihuatl Serrano Ángel,Daniel Segura,Jeiry Toribio Jiménez,Miguel Ángel Rodríguez Barrera,Carlos Ortuño Pineda,Yanet Romero Ramírez 한국미생물·생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.2
The global carbon storage regulator (Csr) system is conserved in bacteria and functions as a regulator in the exponential and stationary phases of growth in batch culture. The Csr system plays a role in the central carbon metabolism, virulence, motility, resistance to oxidative stress, and biofilm formation. Although the Csr was extensively studied in Gram negative bacteria, it has been reported only in the control of motility in Bacillus subtilis among Gram positive bacteria. The goal of this study was to explore the role of the csrA gene of Bacillus licheniformis M2-7 on motility and the bacterial ability to use hydrocarbons as carbon source. We deleted the csrA gene of B. licheniformis M2-7 using the plasmid pCsr-L, harboring the spectinomycin cassette obtained from the plasmid pHP45-omega2. Mutants were grown on culture medium supplemented with 2% glucose or 0.1% gasoline and motility was assessed by electron microscopy. We observed that CsrA negatively regulates motility by controlling the expression of the hag gene and the synthesis of flagellin. Notably, we showed the ability of B. licheniformis to use gasoline as a unique carbon source. Our results demonstrated that CsrA is an indispensable regulator for the growth of B. licheniformis M2-7 on gasoline.