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        Medium Optimization using Mathematical Statistics for Production of β-Carotene by Blakeslea trispora and Fermenting Process Regulation

        Zuowen Yan,Changgao Wang,Jianguo Lin,Jun Cai 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.6

        Optimization of medium components forenhancement of β-carotene production by Blakesleatrispora was achieved using mathematical statistics. Optimum concentrations of components using Plackett-Burman design and response surface methodology (RSM)were D-glucose 7.16%, wheat bran extract 4.08%, MgSO40.04%, soybean oil 3%, thiamine 0.01%, soybean meal1%, and KH2PO4 0.2%. Maximum production of β-carotene using optimized medium was 643 mg/L in ashake flask. A predicted value of 669 mg/L was based onresults of an RSM regression. The optimum aeration rate of1.5 vvm produced 866 mg/L and the optimum agitationspeed of 100 rpm produced 975 mg/L of β-carotene. The tof a quadratic model using regression derived coefficientswas significant. Maximum production of β-carotene usingthe optimized medium in a stirred tank reactor (10 L) at anoptimal aeration rate and an optimum agitation speed withaddition of 0.1%(v/v) β-ionone was 1,357 mg/L.

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