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Antioxidant Activity of Nudibranch (Melibe viridis) Glycosaminoglycans
Zuliyati Rohmah,Bernadeth F. Ticar,Si-Hyang Park,Yeung Joon Choi,Seok-Jeong Kang,Byeong-dae Choi 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
The glycosaminoglycans (GAGs) from Melibe viridis were extracted and optimized using Multifactorial design. Four factors (Temperature, pH, incubation period, and enzyme ratio) were varied in the optimization experiment. The design used three enzymes namely; Alcalase, Flavourzyme, and Protamex. The optimum results were obtained using Flavourzyme at pH 8.0, incubation temperature of 450C, for 15 hours, and an enzyme ratio of 1.5% relative to sample weight. The extracted Melibe GAGs (prepared at different concentrations) were tested for DPPH scavenging effect, it showed that, it has a comparable activity with standard, the Ascorbic acid. It also showed a reductive capability in a concentration-dependent manner. For the hydroxyl radical scavenging effect, the results showed that the activity is very near with that of the standard too, butylated hydroxyanisole (BHA). From the data obtained in this study, it can be inferred that, Melibe GAGs has a potential in the field of medicine as well as well as they can be used as dietary supplements with specific functional properties
Atopic Skin Inflammation Improved by Using Extracts of Seaweed and Ascidians Tunic
B. Munkhjargal,Zuliyati Rohmah,Bernadeth Ticar,Lee Su Sun,Park Si Hyang,Choi Soon Ok,Byeong Dae Choi 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
In a recent day, the environmental pollution caused by industrialization, usage of food additives increased and resulting the several types of atopic symptoms. We report the successful application of seaweed and ascidians tunic used as mediator for preventing skin inflammation of human. The current study was investigated to treat the skin inflammation; raw seaweed and ascidians tunic have been used and extracted. A natural ascidians tunic was extracted to get the glycosaminoglycans (GAGs). GAGs were mixed with the extraction of natural seaweed and it tested on the subject. As a result, it is found that the extraction of seaweed and ascidians tunic together refined material was not involved the cytokine inhibitory, although an extraction of seaweed was showed the anti-inflammation effect by the experiment. Ascidians tunic is represents an effect of cell generation and the mechanism of cytokines inhibition are rather than the inflammatory inhibition, at the same time the skin regeneration is considered greater effect. Finally, we found that the atopic skin inflammation can be improved and/or treated by using natural mixture extracts of the seaweed and ascidians tunic.