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- 저자 : Zhen Guohua (검색결과 4 건)
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( Jing Liu ),( Zhen Li ),( Guohua Yu ),( Ting Wang ),( Guimei Qu ),( Yunhui Wang ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.10
To clarify the role of long intergenic nonprotein-coding RNA 1232 (LINC01232) in the progression of gastric cancer and the potential mechanism, we analyzed the expression of LINC01232 in TCGA database using the GEPIA online tool, and the LINC01232 level in gastric cancer cell lines was detected by quantitative real time-polymerase chain reaction (qRT-PCR) as well. Cell proliferation assay, colony formation assay, transwell assay and tumor formation experiment in nude mice were conducted to observe the biological behavior changes of gastric cancer cells through the influence of LINC01232 knockdown. LncATLAS database and subcellular isolation assay were used for subcellular distribution of LINC01232 in gastric cancer cells. The interaction among LINC01232, zeste homolog 2 (EZH2) and kruppel-like factor 2 (KLF2) was clarified by RNA-protein interaction prediction (RPISeq), RNA immunoprecipitation (RIP), qRT-PCR and chromatin immunoprecipitation (ChIP) assay. Rescue experiments were further conducted to elucidate the biological function of LINC01232/KLF2 axis in the progression of gastric cancer. LINC01232 was upregulated in stomach adenocarcinoma (STAD) tissues and gastric cancer lines. LINC01232 knockdown inhibited the proliferative capacities of gastric cancer cells in vitro, and impaired in vivo tumorigenicity. LINC01232 was mainly distributed in the cell nucleus where it epigenetically repressed KLF2 expression via binding to the enhancer of EZH2, which was capable of binding to promoter regions of KLF2 to induce histone H3 lysine 27 trimethylation (H3K27me3). LINC01232 exerts oncogenic activities in gastric cancer via inhibition of KLF2, and therefore, the knockdown of KLF2 could reverse the regulatory effect of LINC01232 in the proliferative ability of gastric cancer cells.
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CRASHWORTHINESS OPTIMIZATION OF TAPERED UD-CFRP TUBE ACCOUNTING FOR MULTIPLE LOADING PANGLES
Chen Yisong,Zhu Guohua,Wang Zhen 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.4
Tapered composite energy-absorbing components have superior advantages in weight reduction and crashworthiness improvement subjected to oblique compressions compared to straight structures. This study investigated the crashworthiness characteristics of uni-directional carbon fiber reinforced plastic (UD-CFRP) tubes under various loading angles, and further provided the guidance on multi-objectives optimization for UD-CFRP tubes accounting for multiple loading cases. The crashworthiness characteristics of straight UD-CFRP tubes subjected to three compressive angles (0°, 10° and 20°) were firstly explored experimentally, and results indicated that energy-absorbing capacity of samples decreased with the loading angles increasing due to changes in deformation behaviors. The multi-layer finite element modes (FEMs) were developed and validated, and simulations found that internal energy (IE) of intra-CFRP layer and inter-cohesive layer, friction energy decreased with the increase in loading angles. Parametric studies indicated crashworthy performances of UD-CFRP samples under multiple loading angles can be further improved by adjusting the tapered angle or wall thickness. Consequently, the synthetic special energy absorption (SEAβ), synthetic peak crushing force (PCFβ) and mass of tapered tube were optimized accounting for three different loading groups. Compared to baseline sample, the SEAβ was improved by 14 %, while the PCFβ and mass were reduced by 30.2 % and 19 %, respectively.
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Rediscovering Impacts of Collaboration through Workflow Analysis to Value Service Development
Chengxiang Ren,Yucong Duan,Zhangbing Zhou,Guohua Fu,Zhen Guo,Honghao Gao 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.11
Information and service economies are developing rapidly in recent years with the development of information technology and new applications. Software and service development are becoming more and more complex relating to different participants, organizations and alliances. This phenomenon has caused urgent requirements on economic and technical fusion through coordination and collaboration among stakeholders. This paper addresses the gap between business layer and technical development in roles and activities. We then propose a combination of economic value and profits and technical service development processes. We also consider value exchange under profit sharing contracts in participants' workflows. A simulation and an experiment are performed to show related impacts.
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Liu Na,Feng Yuchen,Liu Huicheng,Wu Wenliang,Liang Yuxia,Li Pingfei,Wei Zhengping,Wu Min,Tang Zhao-Hui,Han Junyan,Cheng Xiang,Liu Zheng,Laurence Arian,Li Huabin,Zhen Guohua,Yang Xiang-Ping 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.3
Purpose Macrophages are important regulators of environmental allergen-induced airway inflammation and asthma. ATP6V0d2 is a subunit of vacuolar ATPase highly expressed in macrophages. However, the functions of ATP6V0d2 in the regulation of pathogenesis of allergic asthma remain unclear. The aim of this study is to determine the function and related molecular mechanisms of macrophage protein ATP6V0d2 in allergic asthma. Methods We compared the disease severity between female C57BL/6 wild-type and ATP6V0d2−/− mice in an ovalbumin (OVA)-induced asthma model. We also investigated the association of expression of ATP6V0d2, PU.1 and CCL17 with disease severity among asthmatic patients. Results The expression of ATP6V0d2 in sputum cells of asthmatic patients and in the lungs of OVA-challenged mice was enhanced compared to healthy subjects and their counterparts, respectively. However, ATP6V0d2-deficient mice exaggerated inflammatory cell infiltration as well as enhanced alternative activated macrophage (AAM) polarization and mucus production in an OVA-induced asthma model. Furthermore, we found that Atp6v0d2 promoted lysosomal degradation of Pu.1, which induced AAM polarization and Ccl17 production. Among asthma patients, ATP6V0d2 expression was inversely associated with disease severity, whereas PU.1 and CCL17 expression was positively associated with disease severity. Conclusions Our results identify macrophage Atp6v0d2, as an induced feedback inhibitor of asthma disease severity by promoting Pu.1 lysosomal degradation, which may in turn leads to reduced AAM polarization and Ccl17 production.
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